This seminar discusses genome editing techniques including site-specific recombination using Cre/loxP and Flp/FRT systems and site-specific nucleases such as meganucleases, zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9. CRISPR/Cas9 uses RNA-guided Cas9 nucleases to introduce double-stranded breaks at targeted DNA locations defined by a protospacer sequence, which is matched to repeated sequences in the CRISPR array.

2. seminar
Genome editing
Supervisor
Dr.Razi
Provisioner
Nazila gharibi
winter 1399

4. ➢ by Site- Specific Recombinases )SSRs(
➢ by Site- Specific Nucleases )SSNs(

8. ✓ Cre/loxP
✓ Flp/FRT
Site- specific recombination

11. MegaNuclease
ZFN
Zinc finger nucleases
TALEN
Transcription activator-like
effector nucleases
CRISPER
Clustered regularly interspaced short
palindromic repeats

21. (Protospacers)

23. (Protospacers)
(repeated sequence)