This document discusses gene construct development and outlines several software tools used for gene isolation including Vector NTI, SnapGene, and A Plasmid Editor. It also provides a brief overview of restriction enzyme based cloning, noting that this is a common technique where the same restriction enzymes are used to cut both the vector and insert for ligation, resulting in a cloned insert.

1. Gene Construct Development
Dr Muhammad Asif, PS
Gene Isolation Lab
ABD, NIBGE

3. Vector NTI
SnapGene
A Plasmid Editor (ApE)

4. A Plasmid Editor (ApE)

7. SnapGene

8. How to create, open and import a file in SnapGene

13. Restriction Enzyme based Cloning
• Restriction cloning is a common cloning
technique where restriction enzymes are used
to prepare an insert and a vector for ligation.
Vector and insert should be cut with the same restriction enzymes

15. SELECT VECTOR

17. SELECT INSERT

21. Cloned insert (product)