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Enzymes Form and FunctionCatalytic proteinsLower activation
- 1. Enzymes: Form and Function Catalytic proteins Lower activation energy of reaction Combine or break apart substrates Not used up in reaction End in –ase Lactase metabolizes lactose DNA Polymerase – polymerize nucleotides Substrate to product https://www.khanacademy.org/test- prep/mcat/biomolecules/enzyme-structure-and- function/a/enzyme-structure-and-function 1 Energy of activation with enzyme Energy needed to run reaction without an enzyme Temp & pH Changes in environmental conditions will alter enzyme
- 2. structure, and therefore alter its function Enzyme activity measured by Initial reaction velocity (IRV) Amount of product per time this is what you’re doing in today’s experiment Or could use amount of product per degree (for a temperature graph) what is shown in the graph above IRV = (slope of the graph) Reaction you will run: the reaction goes from clear to brown peroxidase is not used up in reaction guaiacol gets oxidized; hydrogen peroxide gets reduced This is a redox reaction; Think about how rust turns a different color when iron is oxidized Baseline: Filtered water – are the enzyme concentrations too weak/strong? Question: how does pH impact the function of peroxidase? Hypothesis: ______________________________________ _ Experiment: try at pH of 4, 7, and 10 guaiacol (clear) + H2O2 tetraguaiacol (brown) + H20 peroxidase Experiment USE CORRECT PIPETTE FOR EACH CHEMICAL
- 3. Make sure to only take out 300 microliters of Guaiacol 100 microliters to .1 milliliter Get Guaiacol from me Prepare your blank to tare the colorimeter – fill cuvette 2/3 full and make sure the clear side of cuvette is perpendicular to the colorimeter’s light Don’t touch the clear sides (fingerprints are a problem). Micropipettes: Don’t turn upside down. 1st resistance to extract chemical; 2nd resistance to eject chemical. Pay attention to units on pipette. Don’t reuse tips. Before adding enzyme solution to test, have all of your supplies ready: your colorimeter tared and ready to go Guaiacol, pH buffer, and H202 in a test tube stopwatch Once enzyme is added, quickly pour into cuvette and place in colorimeter, recording every 15 sec. Input data into excel sheet for all of class to record Calculate IRV using greatest difference between two adjacent points You will make a line graph of our data using Excel (there will be 4 lines on graph) BRING THIS GRAPH NEXT WEEK TO CHECK CORRECTNESS! We will cover components of the lab report following your lab practical next week You will have access to sample lab reports, rubrics, instructions
- 4. Topic: Effects of pH on Peroxidase upcoming due dates: Materials and Methods – due March 15th 11:59pm on Canvas Literature Review – due April 1st 11:59pm on Canvas Introduction – due April 8th 11:59pm on Canvas entire lab report – due April 22nd 11:59pm on Canvas Lab Report – due March 25th 11:59pm on Canvas using Turnitin Next week: BSC110L LAB PRACTICAL covers labs: Topics 1-4 (scientific method, biomolecules, microscopes and cells, enzymes) worth 20% of your grade! DO NOT arrive late no electronics on your person (phones, ear buds, smartwatches in your bag) Written Exam: 40-45 questions on written portion 55 questions, 2 points each One or two stations for practical applications: I will be monitoring you at these stations and giving you points upon satisfactory completion (10-15 points) when complete: students can either turn their paper in or revisit stations one at a time for a short period Revisit the powerpoints for all of the labs Review the lab manual pages If we thought it was important enough to put in a powerpoint or cover it in a quiz or do it as an activity, it’s important enough to put on the exam! Use the study guide found on Canvas
- 5. Biology Tutoring (Walker Science room 149) Tutoring for BSC 110/111: Monday 8am - 7pm Tuesday 8am - 11am and 2:30pm - 5pm Wednesday 8am - 5pm Thursday 8am - 11am and 2:30pm - 3:30pm Friday 9am - 10:30am Tutoring for BSC 107/250/251: Monday 10am – 12pm Wednesday 10am – 12pm Thursday 5:30pm – 7pm Friday 8am – 12pm A graduate student will be present who can help with lab reports during the following times: Tuesday 10am - 12pm and 2pm - 5pm Wednesday 10am - 12pm and 2pm - 5pm
- 6. Thursday 10am - 12pm and 2pm - 5pm Friday 10am - 12pm From the syllabus: Students arriving more than 15 minutes late for a lab practical will not be allowed to take the practical during the allotted time. If a suitable excuse is provided to the teaching assistant and approved by the lab coordinator, a make- up lab practical may be scheduled. In the event a student misses a lab practical due to family emergency, illness, or University function, contact the Teaching Assistant and Lab Coordinator immediately. If the student does not contact the Teaching Assistant and Lab Coordinator within 48 hours of the exam, the student will not be allowed to make up the missed assessment. In addition, a verified excuse must be obtained from the Assistant to the Vice President of Student Affairs in Union 219 and submitted to the Teaching Assistant and Lab Coordinator within one week of the date of the exam. After review and approval of the excuse, the Teaching Assistant and Lab Coordinator will work with the student to arrange a date and time for a make-up practical. A student is only allowed to make up one lab practical during the semester. If there was no prior contact and/or verified excuse obtained, as explained above, the student will receive a score of zero on any assessment for that day. Today:
- 7. Activities: Enzymes Lab Prepare for the week of the 7th: Study! Prepare for the week of the 21st: Fill out Excel sheet with your data Study for quiz on topics 4 and 5 Prelab for Topic 5: Visualizing Metabolism: Respiration and Photosynthesis Signature Assignment: Change Management Purpose of Assessment Develop specific strategies with supporting tactics to implement positive change within an organization. You may refer to the information that you prepared in Week 5 to complete this assignment, but your submission should add to your prior research and not just copy your Week 5 assignment. Note: Do not copy information from a previous week. Use it as a reference. Scenario: You’ve been hired as a consultant to develop strategies with supporting tactics to implement positive changes for Hendrick Health in Abilene, TX. Review your analysis of the corporation’s change process from Week 5 to be sure you have a thorough understanding of the change and the need for the change. Prepare a Change Management Essay for the Hendrick Health’s Board of Directors. Include the following: Outline of Essay Paragraph 1 Introduction
- 8. Paragraph 2 Overview of Hendrick Health Paragraph 3 What change needs to be made? Paragraph 4, 5, and 6 Evaluate why this change needs to occur. Give three examples. Each example has its own paragraph. Paragraph 7 Discuss hos this change impacts Hendrick Health on a global scale Paragraph 8 Discuss how this change impacts employees Paragraph 9 · Using Kotter’s 8-Step Change Model, Develop strategies for each of the 8 steps in Kotter’s model chart strategies for positively implementing the organizational change. · Create chart and explain. Paragraph 10 · Develop tactics to support each strategy. Chart tactics for positively implementing the organizational change. Create chart and explain. Paragraph 11 · Justify the effectiveness of each strategy and corresponding tactic with a rationale. Paragraph 12 Conclude essay with an explanation of how this positive organizational change will help the company sustain a competitive advantage in the global market. References Format your paper in accordance with APA standards, include in text citations (4 at a minimum), and use four references.