Tuberculosis in India In India, each year, approx. 220,000 deaths are reported due to Tuberculosis. Between 2006 and 2014, the disease cost Indian economy USD 340 billion. This public health problem is the world's largest tuberculosis epidemic. India bears a disproportionately large burden of the world's tuberculosis rates, as it continues to be the biggest health problem in India. Wikipedia

1. End TB Health Afflictions India Plus
via Medical/Social Collab Initiative 2018+
Hydrabad, India Virginia Fairfax
Twin Project
01

2. End TB Health Afflictions India Plus
via Medical/Social Collab Initiative 2018+
Hydrabad, India Virginia Fairfax
Twin Project
Your Link: Linkedin.com/cafetwin 02

3. Public Service Announcment (PSA)
Hydrabad, India Virginia
Fairfax Twin Project
Your Link: Linkedin.com/cafetwin
03

4. End TB Health Afflictions India Plus
via TB Vaccine Initiative 2018+
Med Intervention TB Vaccine Topic:
“Gene silencing by
CRISPR interference”
By Eira Choudhary & Nisheeth Agarwal, Et Al
Vaccine and Infectious Disease Research Center,,
Translational Health Science and Technology Institute
Haryana, India
Abstract Summary: Here we introduce a novel clustered
regularly interspaced short palindromic repeat (CRISPR)
interference (CRISPRi) approach that efficiently represses
expression of target genes in mycobacteria.
CRISPRi thus offers a simple, rapid & cost-effective
tool for selective control of gene expression
in mycobacteria.
Nature Communications volume 6,
Article number: 6267 (2015)
doi:10.1038/ncomms7267
Hydrabad, India Virginia
Fairfax Twin Project
Your Link: Linkedin.com/cafetwin
04

5. uestion: What Does It Take to
uild “Community Wealth” for
nowledge Economy ?
One Possible Answer:
“Lack of Knowledge
is the Problem” W.E. Deming
Fairfax VA Tomorrow
_Quality Living Today Music & Science
Club Twin05

6. End TB Health Afflictions India Plus
via TB Vaccine Initiative 2018+
Med Intervention TB Vaccine Topic:
Abstract Summary: “In particular, the ability
to create conditional knockouts has not been developed.
This is particularly important given the likelihood that
most genes within the small genome of Chlamydia
may be essential. Here, I describe the feasibility of
using CRISPR interference (CRISPRi) based on
the catalytically inactive Cas9 variant (dCas9) of
Staphylococcus aureus to inducibly, and reversibly,
repress gene expression in C. trachomatis.”
Author: Scot P. Ouellette
Department of Pathology & Microbiology,
Univ of Nebraska Medical Center, Omaha, NE, USA
Technology Report ARTICLE Front. Cell. Infect. Microbiol.,
27 February 2018 | https://doi.org/10.3389/fcimb.2018.00059
Hydrabad, India Virginia
Fairfax Twin Project
Your Link: Linkedin.com/cafetwin
Feasibility of a Conditional Knockout
System for Chlamydia Based
on CRISPR Interference
Chlamydia trachomatis
06

7. Mycobacterium tuberculosis is a species
of pathogenic bacteria in the family Mycobacteriaceae
& the causative agent of tuberculosis
Wikipedia It can only be spread through air droplets originating
from a person who has the disease either
coughing, sneezing, speaking, or singing
Wikipedia
Hydrabad, India Virginia
Fairfax Twin Project
Your Link: Linkedin.com/cafetwin
07

8. End TB Health Afflictions India Plus
via Medical/Social Collab Initiative 2018+
Operation Asha
Opasha.org
08

9. End TB Health Afflictions India Plus
via Medical/Social Collab Initiative 2018+
Cultural Harmony
Conservation &
Community Vitality
Club Twin
Urban Crucible
Public Service Announcment (PSA)
Hydrabad, India Virginia
Fairfax Twin Project
Your Link & Mine : Linkedin.com/cafetwin
09

10. End TB Health Afflictions India Plus
via Medical/Social Collab Initiative 2018+
Hydrabad, India Virginia
Fairfax Twin Project
Your Link & Mine
Linkedin.com/cafetwin
10