The document discusses copper peptide (GHK-Cu), a tripeptide that forms a complex with copper ions. It has various cosmetic functions such as promoting collagen production, repairing skin, reducing wrinkles and stimulating hair growth. It works by supplying copper to enzymes involved in tissue repair and antioxidant activities. The document provides details on its mechanism of action, technical specifications, applications and market products containing copper peptide as an active ingredient.

1. www.specchemind.com
Skin Remodeling Factor
Copper Peptide

2. Introduction
Effect
Mechanism of actions
Cosmetic Functions
Technical data sheet
Application
Index

3. Introduction
GHK is a tripeptide possesses a high affinity for copper(II) ions,
with which it spontaneously forms a complex (GHK-Cu).
This peptide was first described as a growth factor for a variety
of differentiated cells.
GHK naturally occurs in human plasma, saliva, and urine. The
plasma GHK level is about 200 ng/mL(10−7M) at age 20, but
declines to 80 ng/mL at age 60.

4. CAS No.： 130120-57-9
INCI Name: Copper tripeptide-1
Mocular Fomula: C14H24N6O4 (GHK)
Mocular weight：742
The molecular structure of the tri-peptide GHK-Copper

5. Mechanism of actions
Copper is essential to vital cellular and enzyme processes required
for human health. It plays an important role in the enzyme
systems which relate to tissue repair, hair growth and other
biological responses.
Copper is essential to Copper-dependent enzymes, including
cytochrome C oxidase (energy production), superoxide
dismutase(antioxidation) and lysyl oxidase (cross-linking of
elastin and collagen).
Research has shown that copper delivered by copper peptides.

7. Cosmetic Functions
GHK promotes extra large collagen aggregates degradation in
scars, regular collagen synthesis in normal skin,
GHK regulates ECM (elastin, proteoglycans and
glycosaminoglycans) production, cell migration, anti-
inflammatory, and antioxidant responses.
Repair and remodel skin, anti-wrinkle, anti-aging, firm skin.
Enlarging hair follicles and stimulating hair growth.

8. Skin Remodeling is the process that removes proteins and older
cells from the skin to smooth skin, remove scars, lesions, and
wrinkles
Skin remodeling is very active in young people but declines
rapidly as human pass age 20.
Increasing skin remodeling is the key to producing a
biologically younger skin.
Cosmetic Functions -- Skin Remodeling

9. • Double blind test.
• 67 volunteers, GHK-Cu vs. placebo were applied twice daily for
12 weeks on facial skin.
Result:
• GHK-Cu Improved skin laxity, clarity and appearance, reduced
fine lines, coarse wrinkles and mottled hyperpigmentation and
increased skin density and thickness.
Reference: Leyden, J.J., Stevens, T., Finkey, M.B. and Barkovic,S. Skin care benefits of copper
peptide containing eye creams. In: American Academy of Dermatology 60th Annual Meeting
(Gaspari, A.A., Goldberg, L.H., Gupta,A.K. et al., ed.), pp. 29, American Academy of
Dermatology,New Orleans, LA, USA (2002).
Cosmetic Functions – Skin Remodeling

10. GHK-Cu was tested in a 12-week placebo controlled study , on
facial skin of 71 women with mild to advanced photodamage.
Result:
• At week 1, compared with placebo, the skin laxity, clarity, fine
lines and overall appearance were improvement.
• At week 2, skin elasticity significantly improved and consistent
with ultrasound increase in overall skin density and thickness.
Reference: Leyden, J.J., Stevens, T., Finkey, M.B. and Barkovic,S. Skin care benefits of copper
peptide containing facial cream. In: American Academy o Dermatology 60th Annual Meeting
(Gaspari, A.A., Goldberg, L.H.,Gupta, A.K. et al., ed.), pp. 29, American Academy of Dermatology,
New Orleans, LA, USA (2002).
Cosmetic Functions – Skin Remodeling

11. GHK-Cu increase hair growth by many ways:
1.Enlarging hair follicles and stimulating hair growth
2.Inhibit DHT expression
3.Improvement scalp damage and inflammation
For Hair growth, GHK-Cu is better than (GHK)2-Cu
Cosmetic Functions – Hair growth

12. Changes Associated with Thinning and
Loss
SOLUTIONS
DHT accumulation
Copper ions are potent inhibitors of 5-
Alpha Reductase
Follicle miniaturization
Copper peptides increase follicle size and
this produces thicker hair.
The follicle's cycle shorten
Copper peptides increase follicle cell
proliferation and decreases programmed
cell death of follicles (apoptosis).
Inflammation
Copper peptides increase superoxide
dismutase expression and reduce damage
caused from interleukin-1, TGF-beta 1,
and reactive carbonyl species.
Scalp damage
Copper peptides help repair scalp
damage.
Hair grays with age
Copper peptides supply copper that
tyrosinase needs to produce melanin.
How Copper Peptides Help Reduce Hair Thinning and Loss
http://www.folligen.com/
Cosmetic Functions – Hair growth

13. Technical data sheet
Source: Synthetic
Apearance: Blue powder
Molecular Ion Mass: 742.29 ± 1.0
Purity (HPLC) (GHK): Not less than 95.0 %
Solubility: High soluble in water
Package:1g,10g,50g,100g/plastic bottle or as customer request.
Storage:2-8℃,dry place,protect from light.
Shelf life:2years.

14. Application
Cosmeceutical applications:
Skin care:
Anti-ageing, anti-wrinkle, After-sun products, after skin resurfacing,
skin moisturizer,.
Hair care:Hair growth stimulator.shampoo,Follicle Therapy
Spray,Revitalizing Shampoo,Restructuring Conditioner.
Usage:
Added at the last step,the tempeature is below 40℃.
Dosage：
0.01-5%.

16. Market products
ingredients for Neova Therapy Creme De La Copper
Water, Capiylic CapricTriglyceride, Butylene Glycol
, Prunus Amygdalus Dulcis (Sweet Almond) Oil, Ste
aric Acid, Simmondsia Chinensis (Jojoba) Seed Oil,
Glyceryl Stcarate, PEG-
100 Stearate, Ricinus Communis (Castor) Seed Oil,
Cetyl Alcohol, Beeswax, Dimethicone, Magnesium
Aluminum Silicate, Prezatide Copper Acetate, Hyd
rogenated Lecithin, Tocopheryl Acetate, Beta Sitoste
rol, Niacin, Calcium Pantothenate, Pyridoxine HCL,
Ascorbyl Palmitate, Retinyl Palmitate, Cholecalcifer
ol, Thiamine Nitrate, Phytonadione, Oyster Shell Ext
ract, Sea Silt Extract, Vims Vinifera (Grape) Seed Ex
tract, Allantoin, Benzophenone-
4, Triethanolamine, Sodium Metabisulfite, Xanthan
Gum, Propylene Glycol, Polysorbate 80, Phenoxyeth
anol, Methylparaben, Ethylparaben, Butylparaben, Pr
opylparaben, Isobutylparaben

17. ingredients for Neova Complex CU3 Intensive Tissue
Repair Creme
Petrolatum, Water (Aqua), Microcrystalline Wax, Par
affin, Mineral Oil, Glycerin, PVP Eiconsene Coplyme
r, Hydroxypropyl Methylcellulose, Benzyl Alcohol, P
rezatide Copper Acetate [Bis (Tripeptide-
1) Copper Acetate], Sodium Chloride.

18. ingredients for Osmotics Blue Copper 5 Prime
Eye
Water, Sodium Hyaluronate, Dimethicone,
Medicago Sativa (Alfalfa) Seed Extract,
Hydrolyzed Lupine Protein, Plankton Extract,
Glycerin, Dimethiconol, Albizia Julibrissin
Bark Extract, Darutoside, Butylene Glycol,
Carbomer, Polysorbate 20, Palmitoyl
Tripeptide-1, Palmitoyl Tetrapeptide-7, Acetyl
Tetrapeptide-2, Caprylyl Glycol, Saccharide
Isomerate, Prezatide Copper Acetate, Copper
PCA, Mica, Titanium Dioxide,
Hydroxyethylcellulose, Diazolidinyl Urea,
Iodopropynyl Butylcarbamate, Potassium
Sorbate, Tromethamine.

19. ingredients for DS Laboratories Spectral DNC-N（hair care)
Deionized water, propylene glycol, nanoxidil (5%), PEG-
40 hydrogenate castor oil, glycerin, adenosine, lactic acid, 2,4 diamino pyramidine-
3 oxide (aminexil), phospholipids (nanosomes), lecithin, retinol, dextran, hydroxypropyl
guar, hydroxypropyl trimonium chloride, procyanidin B2, sophora flavescens, butylene g
lycol, malus domestica fruit cell culture stem cells, acetyl tetrapeptide-
3, polysorbate60, prezatide copper acetate (copper peptide), biotinoyl tripeptide-
1, trifolium pratense flower extract, xanthan gum, myristol pentapeptide-17, thymosin
B4, phenoxyethanol, caprylyl glycol

20. Neutrogena Visibly Firm Night Cream with Active Copper
Tricomin Solution Follicle Therapy Spray

21. Reference
85. Appa, Y., Stephens, T., Barkovic, S. and Finkey, M.B.A clinical evaluation of a copper-peptide-
containing liquid foundation and cream concealer designed for improving skin condition. In:
American Academy of Dermatology 60th Annual Meeting (Gaspan, A.A.,Goldberg, L.H., gupta,
A.K. et al., ed.), p. 28. American Academy of Dermatology, New Orleans, LA,USA (2002).
86. Leyden, J.J., Stevens, T., Finkey, M.B. and Barkovic,S. Skin care benefits of copper peptide
containing facial cream. In: American Academy of Dermatology 60th Annual Meeting (Gaspari,
A.A., Goldberg, L.H.,
Gupta, A.K. et al., ed.), pp. 29, American Academy of Dermatology, New Orleans, LA, USA (2002).
87. Finkey, M.B., Appa, Y. and Bhandarkar, S. Copperpeptide and skin. In: Cosmeceuticals and
Active Cosmetics, 2nd edn (Elsner, P. and Maibach, H.I., eds),pp. 549–564. Marcel Dekker, New
York, NY(2005).
88. Leyden, J.J., Stevens, T., Finkey, M.B. and Barkovic,S. Skin care benefits of copper peptide
containing eye creams. In: American Academy of Dermatology 60th Annual Meeting (Gaspari,
A.A., Goldberg, L.H., Gupta,A.K. et al., ed.), pp. 29, American Academy of Dermatology,New
Orleans, LA, USA (2002).

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