Disinfection Classification Evaluation methods

1. DISINFECTION
Presented by :
Harshita Singhai
Department Of Pharmaceutical Sciences
Dr. Harisingh Gour Vishwavidyalaya Sagar (M.P.)
( A Central University )

2. DISINFECTION
• The process of destruction or removal of
pathogenic microorganisms is termed as
disinfection.
• The object on which disinfection is carried out
is said to be disinfected.

6. LEGAL PROVISIONS
a. BLACK FLUIDS – These are homogeneous dark brown solution of
coal tar acids or similar acids derived from petroleum with or
without hydrocarbon , end /or other phenolic compounds , and
their derivatives and a suitable emulsifier .
b. WHITE FLUID – These are finely dispersed homogeneous white to
off white emulsions of coal tar acids or similar acids derived from
petroleum with or without hydrocarbon , end /or other phenolic
compounds , and their derivatives .
GRADATION
These disinfectants are graded on the basis of the minimum
requirement in respect of Radial Walker (RW) coefficient as -
GRADE R W COEFFICIENT
1 18
2 10
3 5

11. Desirable Properties Of Disinfectants
• Should be effective in low concentration
• Efficacy should not be reduced in presence of blood, faeces and other
organic material
• Non toxic
• Soluble
• Phase separation
• Economic
Factors affecting Disinfectant action
• Concentration
• Time of contact
• Temperature
• Presence of organic matter
• pH

12. Evaluation Of Disinfectants
• Tube dilution or agar plate method
• Filter paper or cup plate method
• Ditch plate method
• Redial Walker
• Chick Martin
• Kelsey Sykes test
• Used dilution test