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Describe how partial diploids can be produced in E. coli.
Solution
Patial diploids can be produced by the transfer of genetic material of Hfr cell to an F- cell
through conjugation.When Hfr mates with F- cell through conjugation the chromosome acts as a
rolling circle transferring part of its genetic material across a conjugation bridge.Very rarely
whole portion of DNA is transferred,resulting very low chance of passing of F factor to the F-
cell.After that F- cell will contain part of Hfr,the exogene and its own genomic material the
endogene,at this stage the cell is called partial diploid.

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