This was presented on idea based competition on DNA Day celebration at University of Dhaka, Bangladesh.

1. ‘DNA DAY
CELEBRATION –2018’

2. Md. Arko Ayon Chowdhury
Saima Akhter Sadia
Rimon Kumar Saha
Israt Jahan
Nazmul Islam Bhuiyan
Corresponding Authors :
PETase – The way to
Clean and Green Bangladesh
Students
BS. Hons. 3rd Year
Department of Biochemistry and Molecular Biology
University of Dhaka

4. Why are we concerned
about plastic ?

5. ‘Polution Through plastic in Bangladesh’

6. Statistical data of Plastic production
In Bangladesh
 1.4 Lacs tons of PET resins is imported every year
 400 crores of bottle produced per year
 Plastic consumption 5 kg/person/year
Ref: Daily Star, 2016

7. PET
(Polyethelene terephthalate)
• Versatile use
• Low cost
• Stable
• Most abundant Plastic
• Easily Processable
It’s non-biodegradable property becomes
a great environmental threat

8. Some bacteria think
Plastic is fantastic !!!

10. MECHANISM OF ‘PETase & MHETase’
Bacteria adhere to
PET
PETase secreted
PETase breaks PET into MHET
MHET uptaken by Bacteria
MHETase breaks MHET into
TPA & EG
TPA used as a carbon source
*PET = Polyethylene Terephthalate
*MHET = Mono-(2-hydroxyethyl) terephthalate
*TPA = Terephthalic acid
*EG = Ethylene Glycol
These Enzymes Activity:
pH range
15-42 (optimally at pH 7-7.5)
Temperature range
15-42 ºC (optimally at 30-37 ºC)

11. Recombination of Bacterial DNA
Isolation of PETase, MHETase &
associated transporter genes from the
Bacterial strain Ideonella sakaiensis 201 F6
Site directed mutations to get more active
enzyme
Transfer of genes to the viable bacteria
from plastic landfill of our country
Transferring of the genes will be done by
Biotechnology of DNA recombination.

12. Finding Out The Viable Bacteria
Adhere to PET
Colonize on the surface of
PET
Express the gene
Take up the gene of PETase
May use TPA as carbon
source for nutrition
Transport PETase outside
The Bacteria
with the
properties

13. Implementation
We will implement the bacteria to the landfill. After confirming-
 Adequate proliferation and growth of the bacterial cell even after the DNA recombination
 The transgenic bacteria can fulfill it’s nutritional requirement from the landfill

14. FUTURE GOAL
 Reducing the implementation cost
 Further research to enhance the activity of enzymes
 Searching for other enzymes that can degrade other plastics
 Involvement of Government & other organization to implement this in large
scale
 Increasing public awareness
 Planning a scheme for over all sustainable waste management program

15. References:
https://www.nature.com/articles/s41467-018-02881-1
https://www.ncbi.nlm.nih.gov/pubmed/27045688
http://www.sci-news.com/biology/ideonella-sakaiensis-bacterium-can-break-down-metabolize-plastic-
03693.html
http://science.sciencemag.org/content/351/6278/1154.full
https://www.livescience.com/62328-plastic-eating-enzyme.html
http://www.pnas.org/content/early/2018/04/16/1718804115

16. ACKNOWLEDGEMENT
Dr. Zeba Islam Seraj
Professor
Department of Biochemistry and Molecular Biology
University of Dhaka
Dr. Md. Rakibul Islam
Associate Professor
Department of Biochemistry and Molecular Biology
University of Dhaka

17. Than
k YOU