CHITINASE AS THE MOST IMPORTANT SECONDARY METABOLITES OF STREPTOMYCES BACTERISIJSIT Editor
Fungal phytopathogens pose serious problems worldwide in the cultivation of economi cally
important plants.
Chemical fungicides are extensively used in current agriculture.However, excessive use of chemical
fungicides in agriculture has led to deteriorating human health , environmental pollution, damaged to
ecosystem and development of pathogen resistance to fungicide.
Because of the worsening problems in fungal disease control , a serious search is needed to identify
alternative methods for plant protection, which are less dependent on chemicals and are more
environmentally friendly. Microbial antagonists are widely used for the biocontrol of fungal plant diseases.
Many species of actinomycates, particulary those belonging to the genus sterptomyces, are well known as
antifungal biocontrol agents that inhibit several plant pathogenic fungi.
Another way biological control has been developed as an alternative of chemicals to tock with plant
pathogenic fungi. Considering high presence of chitin in fungal cell wall, chitinase enzyme is camped as an
effective biocontrol agent against phytopathogenic fungi. Streptomyces bacteria are able to produce various chitinase enzymes, chitinases produced by streptomyces belong to the families 18 and 19 glycosyl hydrolases.
The antifungal activity is mostly shown by fomily 19 Chitinases. In comparison with bacterial family 18
chitinases, the specific hydrolyzing activity of chitinase 19 against soluble and in soluble chitinous substrates
has been markedly higher. Considering the importance of family to investigate antifungal potential of
streptomyces bacteria isolated from east Azarbijan region soils based on molecular identification of family 19
chitinase. encoding gene in these bacteria.
To aim the purpose 110 soil samples were collected from East Azarbaijan and 310 strepomyces
isolates were selected using macroscopic and microscopic observations. DNA genomic of all of the isolates
were extracted and PCR reactions was done using chitinase 19 designed primers as marker.
Totally isolates were selected with molecular selection and antagonistic test were done. One of the isolates
exhibit the most strong antifungal activity.
The strain was identified using 16srDNA gene, and the chitinase encoding gene were amplified partially to
prove the PCR selection. Finally the bacterium were introduced as potentially biological fertilizer.
CHITINASE AS THE MOST IMPORTANT SECONDARY METABOLITES OF STREPTOMYCES BACTERISIJSIT Editor
Fungal phytopathogens pose serious problems worldwide in the cultivation of economi cally
important plants.
Chemical fungicides are extensively used in current agriculture.However, excessive use of chemical
fungicides in agriculture has led to deteriorating human health , environmental pollution, damaged to
ecosystem and development of pathogen resistance to fungicide.
Because of the worsening problems in fungal disease control , a serious search is needed to identify
alternative methods for plant protection, which are less dependent on chemicals and are more
environmentally friendly. Microbial antagonists are widely used for the biocontrol of fungal plant diseases.
Many species of actinomycates, particulary those belonging to the genus sterptomyces, are well known as
antifungal biocontrol agents that inhibit several plant pathogenic fungi.
Another way biological control has been developed as an alternative of chemicals to tock with plant
pathogenic fungi. Considering high presence of chitin in fungal cell wall, chitinase enzyme is camped as an
effective biocontrol agent against phytopathogenic fungi. Streptomyces bacteria are able to produce various chitinase enzymes, chitinases produced by streptomyces belong to the families 18 and 19 glycosyl hydrolases.
The antifungal activity is mostly shown by fomily 19 Chitinases. In comparison with bacterial family 18
chitinases, the specific hydrolyzing activity of chitinase 19 against soluble and in soluble chitinous substrates
has been markedly higher. Considering the importance of family to investigate antifungal potential of
streptomyces bacteria isolated from east Azarbijan region soils based on molecular identification of family 19
chitinase. encoding gene in these bacteria.
To aim the purpose 110 soil samples were collected from East Azarbaijan and 310 strepomyces
isolates were selected using macroscopic and microscopic observations. DNA genomic of all of the isolates
were extracted and PCR reactions was done using chitinase 19 designed primers as marker.
Totally isolates were selected with molecular selection and antagonistic test were done. One of the isolates
exhibit the most strong antifungal activity.
The strain was identified using 16srDNA gene, and the chitinase encoding gene were amplified partially to
prove the PCR selection. Finally the bacterium were introduced as potentially biological fertilizer.
This book is one of the master pieces of Huzur Faiz e MIllat Alaeh Rehma. He has proved with strong evidence that Huzur Alaeh Salam visited Mairaj with his physical body. It is a great book to defuse those wrong beliefs that Huzur Alaeh salam visited Mairaj in dream. اس کتاب میں حضور فیض ملت علیہ الرحمہ نے دیوبندیوں وہابیوں کے غلط عقیدے کی نفی کرتے ہوئے اہلسنت وجماعت کے عقیدے کا دفاع فرمایا ہے کہ ہمارا عقیدہ ہے کہ سرکار علیہ الصلوۃ والسلام اپنے جسم کے ساتھ معراج پر تشریف لے گئے نہ کہ صرف روحانی طور پر یا خواب میں۔ مستند حوالوں کے ساتھ کتاب علم کا خزانہ ہے
This book is one of the master pieces of Huzur Faiz e Millat Alaeh Rehman. In this book he has proved one of the beliefs of Ahl e Sunnat Wa Jamma'at inface Islam that Kissing the nails of your thumbs on the name of The Holy Prophet Peace and blessing be upon him is not Bida'ah. اس رسالے میں حضور فیض ملت علیہ الرحمہ نے اہلسنت و جماعت کے ایک عقیدے سرکار علیہ الصلوۃ والسلام کے مبارک نام پر انگوٹھے چوم کر آنکھوں سے لگانے کا دفاع کیا ہے اورمستند حوالوں سے اس کو جائز ثابت کیا ہے۔ اور گمراہ فرقوں کے اہلسنت پر اعتراض کو غلط اور بے بنیاد ثابت کیا ہے
A REVIEW ON GREEN ENERGY -A SUSTAINABLE APPROACHIJSIT Editor
The current systems of energy supply and use are clearly not sustainable in terms of economic
environment and society .So there is an urgent need for us to increase energy efficiency, reduce energy
consumption, reduce harmful effects by using fossil fuels, mitigating greenhouse emissions. So it is better to
adopt green energy technology/sustainable energy/clean energy to attain sustainable development. .
Present paper focuses on the green energy/renewable energy technology that can be adopted in
order to achieveSustainable development. Some innovative are also mentioned in this paper
COMPARSION OF ANTIOXIDANT POTENTIAL OF DIMOCARPUS LONGAN LOUR. EXTRACTS AND ...IJSIT Editor
The present study was carried out to evaluate antioxidant activity of Dimocarpus longan stems
extracts and also to investigate the main phytoconstituents in the bio-active extract. N-hexane,
dichloromethane, ethyl acetate and methanol 80% extract were tested for free radical scavenging activity on
model reaction with stable 2,2-diphenyl-1-picrylhydrazyl radical (DPPH). The results showed that ethyl
acetate was the most active one as antioxidant agent and phytochemical analysis of that extract revealed the
presence of triterpenes, flavonoids, tannins and carbohydrates. The results may help to discover new
chemical classes of natural antioxidant substances that could serve as selective agents for infectious diseases.
مسلمان کی سب سے گراں قدر دولت ، ایمان ہے جس کی حفاظت کے لئے ہر وقت کوشاں رہنا ضروری ہے ۔ آج ایمان کے لڑیرے، شیطان کے پیروکار ہماری صفوں میں گھس کر بھر پور طریقے سے گمراہ کرنے میں سرگرداں ہیں ۔قرآن وحدیث دکھا دکھا کر، آیات واحادیث کے حقیقی معنی سے کتمان چشم کرکے گمراہیت ، نجدیت ، وھابیت اور دیوبندیت کو فروغ دینے کی اپنی سی کوشش کررہے ہیں ۔ ان بدمذھبوں کے عقائد سے واقفیت انتہائی ضروری ہے ۔ الحمد للہ عزوجل علماء حقہ علمائے اہلسنت نے اس سلسلے میں کتب تحریر فرمائیں اور مسلمانوں کو باخبر رکھنے کی کوشش کی ۔
زیر نظر کتاب المصباح الجدید ( حق وباطل کا فرق) بھی اس سلسلے کی ایک کڑی ہے لیکن اس کتاب کی افادیت اپنی جگہ مسلم ہے جس کا اندازہ قاری کو اس کتاب کے مطالعہ سے ہوجائے گا ۔ مصنف علیہ الرحمۃ نے نہایت آسان انداز میں عوام کی ذہنی سطح کے مطابق اسے سوال وجواب کے انداز میں مرتب کیا ہے ۔
اس کتاب کی سب سے اہم خصوصیت یہ ہے کہ ہر سوال کا جواب دیوبندیوں کے اپنے علماء کی کتب سے باحوالہ دیئے ہیں جس سے ان کے باطل اور کفریہ عقائد کی حقیقت کھل کر سامنے آگئی۔
THE INFLUENCE OF SILICONE ANTIFOAM FROM LEATHER AND DYING WASTE WATER EFFLUE...IJSIT Editor
This study investigates the influence of silicone antifoam agent on waste water from Gashash leather
and Nigerian Spinning and Dying industries (NSD). Waste water from the outlet of the industries were
collected and analyzed for physicochemical parameters. Silicone antifoam was added to the wastewater to
determine the impact of the silicone antifoam on turbidity and chemical oxygen demand (COD)
concentrations. The result shows that both turbidity and COD values significantly increased even when small
concentration of the silicone antifoam was added. Further, independent t-test was used to identify the
variance between the mean value of the wastewater from leather, spinning and dying industries, the results
indicated that there are no significant differences (observed t 0.544, critical t 2.015, and p value 0.589)
between the waste water in leather and dying industries.
This book is one of the master pieces of Huzur Faiz e MIllat Alaeh Rehma. He has proved with strong evidence that Huzur Alaeh Salam visited Mairaj with his physical body. It is a great book to defuse those wrong beliefs that Huzur Alaeh salam visited Mairaj in dream. اس کتاب میں حضور فیض ملت علیہ الرحمہ نے دیوبندیوں وہابیوں کے غلط عقیدے کی نفی کرتے ہوئے اہلسنت وجماعت کے عقیدے کا دفاع فرمایا ہے کہ ہمارا عقیدہ ہے کہ سرکار علیہ الصلوۃ والسلام اپنے جسم کے ساتھ معراج پر تشریف لے گئے نہ کہ صرف روحانی طور پر یا خواب میں۔ مستند حوالوں کے ساتھ کتاب علم کا خزانہ ہے
This book is one of the master pieces of Huzur Faiz e Millat Alaeh Rehman. In this book he has proved one of the beliefs of Ahl e Sunnat Wa Jamma'at inface Islam that Kissing the nails of your thumbs on the name of The Holy Prophet Peace and blessing be upon him is not Bida'ah. اس رسالے میں حضور فیض ملت علیہ الرحمہ نے اہلسنت و جماعت کے ایک عقیدے سرکار علیہ الصلوۃ والسلام کے مبارک نام پر انگوٹھے چوم کر آنکھوں سے لگانے کا دفاع کیا ہے اورمستند حوالوں سے اس کو جائز ثابت کیا ہے۔ اور گمراہ فرقوں کے اہلسنت پر اعتراض کو غلط اور بے بنیاد ثابت کیا ہے
A REVIEW ON GREEN ENERGY -A SUSTAINABLE APPROACHIJSIT Editor
The current systems of energy supply and use are clearly not sustainable in terms of economic
environment and society .So there is an urgent need for us to increase energy efficiency, reduce energy
consumption, reduce harmful effects by using fossil fuels, mitigating greenhouse emissions. So it is better to
adopt green energy technology/sustainable energy/clean energy to attain sustainable development. .
Present paper focuses on the green energy/renewable energy technology that can be adopted in
order to achieveSustainable development. Some innovative are also mentioned in this paper
COMPARSION OF ANTIOXIDANT POTENTIAL OF DIMOCARPUS LONGAN LOUR. EXTRACTS AND ...IJSIT Editor
The present study was carried out to evaluate antioxidant activity of Dimocarpus longan stems
extracts and also to investigate the main phytoconstituents in the bio-active extract. N-hexane,
dichloromethane, ethyl acetate and methanol 80% extract were tested for free radical scavenging activity on
model reaction with stable 2,2-diphenyl-1-picrylhydrazyl radical (DPPH). The results showed that ethyl
acetate was the most active one as antioxidant agent and phytochemical analysis of that extract revealed the
presence of triterpenes, flavonoids, tannins and carbohydrates. The results may help to discover new
chemical classes of natural antioxidant substances that could serve as selective agents for infectious diseases.
مسلمان کی سب سے گراں قدر دولت ، ایمان ہے جس کی حفاظت کے لئے ہر وقت کوشاں رہنا ضروری ہے ۔ آج ایمان کے لڑیرے، شیطان کے پیروکار ہماری صفوں میں گھس کر بھر پور طریقے سے گمراہ کرنے میں سرگرداں ہیں ۔قرآن وحدیث دکھا دکھا کر، آیات واحادیث کے حقیقی معنی سے کتمان چشم کرکے گمراہیت ، نجدیت ، وھابیت اور دیوبندیت کو فروغ دینے کی اپنی سی کوشش کررہے ہیں ۔ ان بدمذھبوں کے عقائد سے واقفیت انتہائی ضروری ہے ۔ الحمد للہ عزوجل علماء حقہ علمائے اہلسنت نے اس سلسلے میں کتب تحریر فرمائیں اور مسلمانوں کو باخبر رکھنے کی کوشش کی ۔
زیر نظر کتاب المصباح الجدید ( حق وباطل کا فرق) بھی اس سلسلے کی ایک کڑی ہے لیکن اس کتاب کی افادیت اپنی جگہ مسلم ہے جس کا اندازہ قاری کو اس کتاب کے مطالعہ سے ہوجائے گا ۔ مصنف علیہ الرحمۃ نے نہایت آسان انداز میں عوام کی ذہنی سطح کے مطابق اسے سوال وجواب کے انداز میں مرتب کیا ہے ۔
اس کتاب کی سب سے اہم خصوصیت یہ ہے کہ ہر سوال کا جواب دیوبندیوں کے اپنے علماء کی کتب سے باحوالہ دیئے ہیں جس سے ان کے باطل اور کفریہ عقائد کی حقیقت کھل کر سامنے آگئی۔
THE INFLUENCE OF SILICONE ANTIFOAM FROM LEATHER AND DYING WASTE WATER EFFLUE...IJSIT Editor
This study investigates the influence of silicone antifoam agent on waste water from Gashash leather
and Nigerian Spinning and Dying industries (NSD). Waste water from the outlet of the industries were
collected and analyzed for physicochemical parameters. Silicone antifoam was added to the wastewater to
determine the impact of the silicone antifoam on turbidity and chemical oxygen demand (COD)
concentrations. The result shows that both turbidity and COD values significantly increased even when small
concentration of the silicone antifoam was added. Further, independent t-test was used to identify the
variance between the mean value of the wastewater from leather, spinning and dying industries, the results
indicated that there are no significant differences (observed t 0.544, critical t 2.015, and p value 0.589)
between the waste water in leather and dying industries.
کیا فرماتے ہیں علمائے دین اس مسئلے میں کہ سرورکائنات فخر موجودات رسول خدا محمدمصطفی صلی اللہ تعالٰی علیہ وآلہٖ وسلم کے ماں باپ آدم علٰی نبینا وعلیہ السلام تک مومن تھے یا نہیں؟بینوا توجروا۔(عہ۱) (بیان کرو اجر پاؤگے ۔ت)
أفضل 11 موقع لعمل اختبارات إلكترونية (Slide Decks).pdfqorrectdm
مع تطور التكنولوجيا، أصبحت أنظمة وأدوات الامتحانات الإلكترونية جزءاً أساسياً من التعليم الحديث. في هذا العرض، سنستعرض أفضل الأنظمة والأدوات التي تساعد المؤسسات التعليمية على تحسين عمليات الامتحان وتقديم تجربة تعليمية متميزة.
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