Demonstration of alternate scanning LCMS for simultanous acquisition of precursor and product ions without precursor mass selection (ie Multiplex LCMS).
Creative Biolabs is skilled in NGS-based cancer research. We support one-stop cancer research services based on a variety of sequencing technologies. Our whole gene sequencing (WGS) and whole exome sequencing (WES) platforms enable us to comprehensively analyze and identify known and unknown cancer gene mutations. Besides, we also provide high-quality target sequencing services, allowing us to analyze several or even hundreds of target gene mutations economically and effectively. Our services will facilitate our customers’ cancer research including cancer diagnosis and treatment.
https://www.creative-biolabs.com/suprecision/genetic-testing-for-cancer.htm
Connector Corner: Automate dynamic content and events by pushing a buttonDianaGray10
Here is something new! In our next Connector Corner webinar, we will demonstrate how you can use a single workflow to:
Create a campaign using Mailchimp with merge tags/fields
Send an interactive Slack channel message (using buttons)
Have the message received by managers and peers along with a test email for review
But there’s more:
In a second workflow supporting the same use case, you’ll see:
Your campaign sent to target colleagues for approval
If the “Approve” button is clicked, a Jira/Zendesk ticket is created for the marketing design team
But—if the “Reject” button is pushed, colleagues will be alerted via Slack message
Join us to learn more about this new, human-in-the-loop capability, brought to you by Integration Service connectors.
And...
Speakers:
Akshay Agnihotri, Product Manager
Charlie Greenberg, Host
Creative Biolabs is skilled in NGS-based cancer research. We support one-stop cancer research services based on a variety of sequencing technologies. Our whole gene sequencing (WGS) and whole exome sequencing (WES) platforms enable us to comprehensively analyze and identify known and unknown cancer gene mutations. Besides, we also provide high-quality target sequencing services, allowing us to analyze several or even hundreds of target gene mutations economically and effectively. Our services will facilitate our customers’ cancer research including cancer diagnosis and treatment.
https://www.creative-biolabs.com/suprecision/genetic-testing-for-cancer.htm
Connector Corner: Automate dynamic content and events by pushing a buttonDianaGray10
Here is something new! In our next Connector Corner webinar, we will demonstrate how you can use a single workflow to:
Create a campaign using Mailchimp with merge tags/fields
Send an interactive Slack channel message (using buttons)
Have the message received by managers and peers along with a test email for review
But there’s more:
In a second workflow supporting the same use case, you’ll see:
Your campaign sent to target colleagues for approval
If the “Approve” button is clicked, a Jira/Zendesk ticket is created for the marketing design team
But—if the “Reject” button is pushed, colleagues will be alerted via Slack message
Join us to learn more about this new, human-in-the-loop capability, brought to you by Integration Service connectors.
And...
Speakers:
Akshay Agnihotri, Product Manager
Charlie Greenberg, Host
Securing your Kubernetes cluster_ a step-by-step guide to success !KatiaHIMEUR1
Today, after several years of existence, an extremely active community and an ultra-dynamic ecosystem, Kubernetes has established itself as the de facto standard in container orchestration. Thanks to a wide range of managed services, it has never been so easy to set up a ready-to-use Kubernetes cluster.
However, this ease of use means that the subject of security in Kubernetes is often left for later, or even neglected. This exposes companies to significant risks.
In this talk, I'll show you step-by-step how to secure your Kubernetes cluster for greater peace of mind and reliability.
Transcript: Selling digital books in 2024: Insights from industry leaders - T...BookNet Canada
The publishing industry has been selling digital audiobooks and ebooks for over a decade and has found its groove. What’s changed? What has stayed the same? Where do we go from here? Join a group of leading sales peers from across the industry for a conversation about the lessons learned since the popularization of digital books, best practices, digital book supply chain management, and more.
Link to video recording: https://bnctechforum.ca/sessions/selling-digital-books-in-2024-insights-from-industry-leaders/
Presented by BookNet Canada on May 28, 2024, with support from the Department of Canadian Heritage.
Encryption in Microsoft 365 - ExpertsLive Netherlands 2024Albert Hoitingh
In this session I delve into the encryption technology used in Microsoft 365 and Microsoft Purview. Including the concepts of Customer Key and Double Key Encryption.
Elevating Tactical DDD Patterns Through Object CalisthenicsDorra BARTAGUIZ
After immersing yourself in the blue book and its red counterpart, attending DDD-focused conferences, and applying tactical patterns, you're left with a crucial question: How do I ensure my design is effective? Tactical patterns within Domain-Driven Design (DDD) serve as guiding principles for creating clear and manageable domain models. However, achieving success with these patterns requires additional guidance. Interestingly, we've observed that a set of constraints initially designed for training purposes remarkably aligns with effective pattern implementation, offering a more ‘mechanical’ approach. Let's explore together how Object Calisthenics can elevate the design of your tactical DDD patterns, offering concrete help for those venturing into DDD for the first time!
JMeter webinar - integration with InfluxDB and GrafanaRTTS
Watch this recorded webinar about real-time monitoring of application performance. See how to integrate Apache JMeter, the open-source leader in performance testing, with InfluxDB, the open-source time-series database, and Grafana, the open-source analytics and visualization application.
In this webinar, we will review the benefits of leveraging InfluxDB and Grafana when executing load tests and demonstrate how these tools are used to visualize performance metrics.
Length: 30 minutes
Session Overview
-------------------------------------------
During this webinar, we will cover the following topics while demonstrating the integrations of JMeter, InfluxDB and Grafana:
- What out-of-the-box solutions are available for real-time monitoring JMeter tests?
- What are the benefits of integrating InfluxDB and Grafana into the load testing stack?
- Which features are provided by Grafana?
- Demonstration of InfluxDB and Grafana using a practice web application
To view the webinar recording, go to:
https://www.rttsweb.com/jmeter-integration-webinar
GraphRAG is All You need? LLM & Knowledge GraphGuy Korland
Guy Korland, CEO and Co-founder of FalkorDB, will review two articles on the integration of language models with knowledge graphs.
1. Unifying Large Language Models and Knowledge Graphs: A Roadmap.
https://arxiv.org/abs/2306.08302
2. Microsoft Research's GraphRAG paper and a review paper on various uses of knowledge graphs:
https://www.microsoft.com/en-us/research/blog/graphrag-unlocking-llm-discovery-on-narrative-private-data/
Accelerate your Kubernetes clusters with Varnish CachingThijs Feryn
A presentation about the usage and availability of Varnish on Kubernetes. This talk explores the capabilities of Varnish caching and shows how to use the Varnish Helm chart to deploy it to Kubernetes.
This presentation was delivered at K8SUG Singapore. See https://feryn.eu/presentations/accelerate-your-kubernetes-clusters-with-varnish-caching-k8sug-singapore-28-2024 for more details.
Slack (or Teams) Automation for Bonterra Impact Management (fka Social Soluti...Jeffrey Haguewood
Sidekick Solutions uses Bonterra Impact Management (fka Social Solutions Apricot) and automation solutions to integrate data for business workflows.
We believe integration and automation are essential to user experience and the promise of efficient work through technology. Automation is the critical ingredient to realizing that full vision. We develop integration products and services for Bonterra Case Management software to support the deployment of automations for a variety of use cases.
This video focuses on the notifications, alerts, and approval requests using Slack for Bonterra Impact Management. The solutions covered in this webinar can also be deployed for Microsoft Teams.
Interested in deploying notification automations for Bonterra Impact Management? Contact us at sales@sidekicksolutionsllc.com to discuss next steps.
The Art of the Pitch: WordPress Relationships and SalesLaura Byrne
Clients don’t know what they don’t know. What web solutions are right for them? How does WordPress come into the picture? How do you make sure you understand scope and timeline? What do you do if sometime changes?
All these questions and more will be explored as we talk about matching clients’ needs with what your agency offers without pulling teeth or pulling your hair out. Practical tips, and strategies for successful relationship building that leads to closing the deal.
The Art of the Pitch: WordPress Relationships and Sales
Asms2004 Alternate Scanning Lcms
1. ASMS Towards Global Proteomics, All Ions all the Time: A Proof-of-Principle of the Qualitative Ion Mapping Technology
2004 Jeffrey C. Silva1, Keith Richardson2, Phillip Young2, Richard Denny2, Kieran Neeson2, Therese McKenna2, Craig Dorschel1, Guo-Zhong Li1, Marc Gorenstein1, Timothy Riley1, and Scott Geromanos1
1
Waters Corporation, Milford, MA USA, 2Waters Corporation, Manchester, UK
Figure 5. As noted in the data processing section, Protein Expression System Informatics Table 1.
Introduction Data Processing Figure 3.
EE 080603_497HL_QuanExpSerial_03 2: TOF MS ES+
uses this principle of chromatographic apex alignment as a first filter to remove
Delta b-ions y"-ions
MSE Protein 100
73.42 BPI
3.53e3
Description Sequence Actual MH+ (ppm) (b1……..bn-1) (y”1…….y”n-1)
Traditional tandem mass spectrometry (MS/MS) is a method of mass spectrometry A basic premise of the Protein Expression System Informatics software is that the PLG 2.0 dB
Sequence
7.13
21.89
22.11 28.11
38.52
42.88
60.94
ions from the elevated energy spectra that may have a retention time similar to, Q96KJ4 Similar to pre-pro-megakarycyte potentiating factor MEVSGDPGIPALHR 1478.7420 2.6 0000000000000 1000000000001
(peptide fragments) 20.82
whereby intact peptides (parent ions) generated from an enzymatic digest of a exact mass MS data acquired at low energy is primarily composed of peptide 46.73
but not coincident with a parent ion of interest (see Poster WPJ 161, Goren- Q9H8J4 CDNA FLJ13564 fis clone PLACE1008201 AGFNSYAELLTHR 1478.7387 0.3 000000000000 100000000000
24.54 33.60
protein sample are mass filtered and collisionally dissociated to yield product or precursor ion information and the exact mass MS data acquired at elevated en- % 6.20 51.80 60.29 stein et al. “Statistical Study of Ions and Peptides Found In LC/MS/MS Analysis of Q9NVE3 CDNA FLJ10787 fis clone NT2RP4000481 ESDDGVVVYVAPTK 1478.7373 -0.6 0000000000000 0000000000000
78.49
fragment ions which are subsequently mass analyzed. The resulting product ion ergy is composed primarily of corresponding peptide product or fragment ion in- Candidate Candidate Peptide Sequence Peptide Protein 7.77 78.99
106.10 Human Serum Digest” and Poster TPR 354, Li et al.). Figure 8 illustrates this Q9BXU7 Ubiquitin carboxyl-terminal hydrolase 26 MTSGNISVSWPATK 1478.7308 -5.0 0000000000000 0000000000000
spectra can be used to determine the sequence of the parent peptide and, Peptide b1 bn Score Assignment
5.13 9.91 67.86 81.71 89.55 95.18 103.24 108.09
formation. It is also understood that fragment ions associated with a particular Mass y”n y”1
19.04
58.51
112.30 117.66 119.79
“cleaning” principle more graphically. The top panel shows the combined ele- P02787 Serotransferrin precursor (Siderophilin) MYLGYEYVTAIR 1478.7348 -2.3 01110000000 10111110111
through a database search, the identity of the originating protein. This method of parent ion are produced simultaneously with the parent ions as they elute from the + Accession #1
0
10.00 20.00 30.00 40.00 50.00 60.00 70.00 80.00 90.00 100.00 110.00 120.00
Time
130.00 vated energy spectra of all ions that elute at 72.425 minutes. The middle panel O60610 Diaphanous protein homolog 1 NSETFPTILEEAK 1478.7373 -0.6 000000000000 000100000000
O60522 Colon cancer antigen NY-CO-45 (Fragment) SALPYENIDSEIK 1478.7373 -0.6 000000000000 000000000000
analysis can be automated and it is generally performed in conjunction with a reverse phase column. 080603_497HL_QuanExpSerial_03 812 (72.425) Cm (812)
267.1570
2: TOF MS ES+
235
shows the elevated energy spectra after being cleaned of ions that do not co-
++++++ Accession #2 100 P50440 Glycine amidinotransferase, mitochondrial precursor WLSMNVLMLDEK 1478.7382 0.0 00000000000 00000000000
real time liquid chromatography separation system. Although this methodology is apex with the low energy doubly charged peptide, m/z 872.54, at 71.96 min-
Q9HB07 MYG1 protein AMDLVQEEFLQR 1478.7308 -5.0 00000000000 10000000000
quite powerful, it has been shown to have limitations when characterizing com- Protein Expression System Informatics filters the data acquired in the alternating ++ Accession #3 utes. Similarly, the bottom panel shows the elevated energy spectra after removal Q9H099 Hypothetical 78 5 kDa protein DLWHAAFFLSGSK 1478.7427 3.0 000000000000 000000000000
plex protein mixtures. Many of these limitations are associated with the serial na- scan experiment in a three-step process. The first step carefully aligns chroma- %
136.0944
295.1492
1071.7020
of ions that do not co-apex with the low energy doubly charged peptide, m/z
++ Accession #4 Q9UNI1 Elastase 1 precursor NSWPSQISLQYR 1478.7387 0.3 00010000000 10000000000
ture of the MS/MS data acquisition process which limits both the qualitative and tographic retention time to associate fragment ions to each appropriate precursor 687.4291
809.5603
1184.7798
1185.7744 739.96, at 72.60 minutes. P21266 Glutathione S transferase Mu 3 VDIIENQVMDFR 1478.7308 -5.0 00000000000 10000000000
670.4338 988.6307 1478.9341
the quantitative reproducibility of the information generated. ion (Figure 1). The second step compares the exact mass of each measured ++ Accession #5 366.1824
1186.7974 1386.7921 1613.9867
1745.0836 In Table 1, the qualitative identification data filtering steps illustrated in Figures P55884 Eukaryotic translation initiation factor 3 subunit 9 (eIF-3 eta) YVVTSVSWWSHK 1478.7427 3.0 01100000000 00000000000
0 m/z
peptide precursor ion to a database containing the accurate mass of every pep- ++++++ Accession #6 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900
2 and 3 have been applied to the 739.96 doubly charged peptide ion eluting
A new method of protein characterization of proteolytically-generated peptides tide (including one missed cleavage) associated with every known protein in the at 72.60 minutes along with its chromatographically associated fragment ions. Table 2.
+ Accession #7 Delta b-ions y"-ions
has been developed which is based on coupling the alternate scanning of pep- proteome under study. Typically, peptides which are within an acceptable mass Figure 6. Each of the proteins listed in Table 1 contains a tryptic peptide sequence that Description Sequence Actual MH+ (ppm) (b1……….…..bn-1) (y”1……….y”n-1)
tides at low and elevated energy1,2 with a robust accurate mass LC/MS data ac- measurement tolerance (within +/- 5 ppm) are identified in a subset peptide data 080603_497HL_QuanExpSerial_03 1: TOF MS ES+ has an accurate mass within plus or minus 5ppm of the 739.96 measured mass. O14775 Guanine nucleotide-binding protein beta subunit 5 MATEGLHENETLASLK 1743.8582 -2.0 011000000000000 100000000000100
Cs 1, 1744.12 71.96 1744.115 0.05Da
quisition mode3. This mode of data acquisition is very high in duty cycle and pro-
100
base (Figure 2). The accurate mass of each y” and b fragment ion is calculated 157
In Tables 1 and 2, columns 5 and 6 indicate those corresponding b- and y”- Q9P2G7 KIAA1380 protein DSSEIPGALWHIYAGK 1743.8701 4.8 010000000000000 100000000000000
vides exact mass MS analysis of both the precursor and product ions for every de- for each of the peptides in this subset database and the measured exact mass Results and Discussion %
0
ions for each peptide, that are found in the elevated energy data to within plus or P13796 L-plastin (Lymphocyte cytosolic protein 1) GDEEGVPAVVIDMSGLR 1743.8582 -2.0 0010000000000000 0000000010000000
080603_497HL_QuanExpSerial_03 1: TOF MS ES+
tectable peptide. Chromatographic retention time is used to associate fragments fragment ion information obtained in the first processing step is compared to this Cs 2, 1744.12 71.96 872.541 0.05Da Q96GG7 Interferon regulatory factor 1 LLEQSEWQPTNVDGK 1743.8548 -4.0 00000000000000 10100100000000
Figures 4 and 5 illustrate typical results from an alternating low and elevated
100
6.87e3 minus 10 ppm mass accuracy and plus or minus 0.05 minutes retention time. A
to their corresponding precursors as a unique attribute of the Waters® Protein Ex- theoretical accurate mass information (Figure 3). A probability-based search
%
“0” at a given amino acid position indicates the corresponding fragment ion was Q9H473 Golgi-spec. brefeldin A-resistance guanine nef-1 NEEIVMPEEQTGLVR 1743.8582 -2.0 00000000000000 00000000000000
collision energy LC/MS analysis of non-depleted human serum tryptic digest. The 0
73.32
pression System technology. Proprietary Protein Expression System Informatics model that takes into account the number, type, and mass accuracy of the meas- 080603_497HL_QuanExpSerial_03
Cs 1, 1630.01
1: TOF MS ES+
not found within the specified search tolerances, and a “1” at a given amino acid
Q9H0K9 Hypothetical 78 6 kDa protein SHPLQLTDDGGFSEIK 1743.8548 -4.0 001000000000000 100000000000000
upper panel of Figure 4 illustrates the low energy base peak intensity chroma- 100
72.68 1630.014 0.05Da
94.4
software has been developed which allows this information to be processed in a ured fragment ions is used to determine the best peptide sequence match. % position indicates the corresponding fragment ion was found within the specified
O75018 Leucocyte Immunoglobulin-like Receptor 6A/B TASHPQDYTVENLIR 1743.8660 2.5 00000001000000 00000000000000
togram and the lower panel shows a low energy exact mass spectrum extracted P01842 Ig lambda chain C regions YAASSYLSLTPEQWK 1743.8588 -1.6 01111111110000 11111111111010
quantitative and qualitative manner. This poster will focus on the qualitative identi- 0
search tolerances. The measured exact mass fragment ion data associated with
at the highlighted retention time of 72.390 minutes. Figure 5 illustrates the same 080603_497HL_QuanExpSerial_03
Cs 2, 1630.01 72.68
1: TOF MS ES+
815.51 0.05Da Q9GZU1 CDNA FLJ22449 fis clone HRC09609 GFLLQNEFVGFMWR 1743.8676 3.4 0000000000000 0000000000000
fication capabilities of this software. Poster TPR 357 (Geromanos et al., Figure 1.
100
3.75e3
the 739.96 peptide parent ion has been compared to the theoretical y” and b
information for the elevated energy function with the example spectrum extracted % O43736 Integral membrane protein 2A (E25 protein) GGEPNFLPVTEEADIR 1743.8548 -4.0 000000010000000 000000000000000
“Towards Global Proteomics by Analysis of Exact Mass Retention Time Pairs: A fragment ions associated with each of the candidate parent ions in Table 1. The
one scan later at 72.425 minutes. While many peptide ions co-elute during this 0
080603_497HL_QuanExpSerial_03 1: TOF MS ES+ Q9Y4J7 Fibroblast growth factor 2-interacting factor NYGILADATEQVGQHK 1743.8660 2.5 000000000000000 110000000000000
proof-of-principle of the Quantitative Ion Mapping Technology”) addresses the 100 Cs 1, 1478.95 72.60 1478.95 0.05Da
measured fragment ions associated with the MYLGYEYVTAIR clearly provide con-
moment in time, only a subset of the peptide ions apex at the same moment in 144 O94935 KIAA0851 protein THLGLIMDGWNSMIR 1743.8669 3.0 00000000100000 00100010000000
quantitative capabilities of the software and Poster TPY 458 (C. Dorschel et %
vincing evidence, with twelve matching fragment ions, to indicate that the 739.96
time. Figure 6 shows the low energy exact mass chromatograms for singly and 0
O94808 Glucosamine-fructose-6P. aminotransferase 2 WATHGVPSAVNSHPQR 1743.8674 3.3 000001000000000 010001100000000
al., “Protocols to Assure Reproducible Quantitative and Qualitative Analysis of 080603_497HL_QuanExpSerial_03
Cs 2, 1478.95 1: TOF MS ES+
doubly charged peptide originates from the Serotransferrin precursor pro-
Collect all precursor ions
doubly charge state ions for 4 example peptides that co-elute in the chroma- 100
72.60 739.957 0.05Da
Tryptic Digests of Complex Protein Mixtures for Global Proteomic Experiments”) 4.57e3
tein. Table 2, illustrates a similar analysis of the 872.54 doubly charged parent Table 3.
tographic region around 72 minutes. The singly and doubly charged ions associ- %
% Coverage Description Median Mass Error % Coverage Description Median Mass Error
highlights the reproducibility of the Protein Expression System platform. 0
ion and its chromatographically associated fragment ions. In this example, there 61 Zinc alpha 2 glycoprotein precursor 1.1 58 Hemoglobin alpha chain 0.2
ated with the same peptide clearly apex at the same chromatographic retention 080603_497HL_QuanExpSerial_03
100
70.82 Cs 1, 1311.92
1: TOF MS ES+
1311.915 0.05Da
12
26
Vitamin D-binding protein precursor
Very low density lipoprotein receptor
3.4
3.7
34
52
Heat shock 70 kDa protein 1
Haptoglobulin-2
6.3