Presentation

1. 1. D-III (2008) : Diploma in Medical Laboratory Technology
(POLKESMAS)
2. D-IV (2011) : Bachelor of Science in Medical Laboratory
Technology (POLKESMAS)
3. S2 (2015) : Master of Science in Clinical Chemistry,
Biomedicine (UNHAS)
1. Laboratory Coordinator and Medical
Laboratory Technology in AURI
Clinical Laboratory Center (2011)
2. Laboratory Supervisor in Parahita
Laboratory Diagnostic Center (2012)
3. Laboratory Coordinator of Clinical
Patology in Hasanuddin University
Hospital (2012-2018)
4. Lecturer & Researcher in Health
Polytechnic of Makassar (2018-Now)

3. SARS-COV-2

4. First 4 Cases were linked
to Huanan Seafood
Marketplace
Start
China CDC was Reported
about the pneumonia
unknown etiology
30th December 2019
29th December 2019 31st December 2019
WHO alerted by Chinese
authorities of a string of
pneumonia cases in
Wuhan (Hubei,Tiongkok)
Huanan Seafood Market
Identified as source of
pneumonia
1st January 2020
9th January 2020
First death from the
2019-nCoV occurs in
Wuhan
7th SARS-Cov-2 was
Isolated by China, 10th first
genome sequence of
SARS-CoV-2 was released
10th January 2020
13th - 16th January2020
13th First infection
occurs outside China,
in Thailand, 16th
First infection
occurs in Japan
20th - 22nd January2020
20th First infection
occurs in South
Korea, 21st First
infection occurs in
Taiwan, 22nd First
infection occurs in
Macau & Hongkong
23rd January 2020
23rd First infection
occurs in Singapore
& Vietnam, 23rd
Wuhan City was
Locked Down
30th January 2019
WHO Declared a “Public
Health Emergency of
International Concern” 27th February 2019 82,294 people from47
courtiers infected
2nd March 2020
First 2
cases
report in
Indonesia
Reference : International Journal of Biological Sciences (SARS-CoV-2 : an Emerging Coronavirus that causes a Global Threat) , Int.J.Biol.Sci. 2020,
Vol.16 (http://wwwijbs.com)

6. Sumber : Gugus Tugas Percepatan Penanganan COVID-19

7. Sumber : Gugus Tugas Percepatan Penanganan COVID-19

8. Sumber : Gugus Tugas Percepatan Penanganan COVID-19

9. Sumber : Gugus Tugas Percepatan Penanganan COVID-19

10. Sumber : Gugus Tugas Percepatan Penanganan COVID-19

11. Sumber : Gugus Tugas Percepatan Penanganan COVID-19

12. Sumber : Gugus Tugas Percepatan Penanganan COVID-19

13. Sumber : Gugus Tugas Percepatan Penanganan COVID-19

14. Visualization of SARS-CoV-2 with Transmission Electron Microscope (TEM)
Reference : Genotype and Phenotype of COVID-19 (Journal of
Microbiology, Immunology, and Infection www.e-jmii.com) 22 March 2020

15. BAT
PANGOLIN
Reference : Probable Pangolin Origin of SARS-CoV-2 Associated with the COVID-19 Outbreak (Current Biology journal), 2020 (https://doi.org/10.1016/j.cub.2020.03.022)

16. Reference : Probable Pangolin Origin of SARS-CoV-2 Associated with the COVID-19 Outbreak (Current Biology journal), 2020 (https://doi.org/10.1016/j.cub.2020.03.022)

17. Reference : Insights into the Recent 2019 Novel Coronavirus (SARS-CoV-2) in Light of Past Human Coronavirus Outbreaks (Pathogens 2020,9,186;doi:10.3390/pathogens9030186),
(www.mdpi.com/journal/pathogens)

18. Reference : COVID-19 : A promising cure for the global panic (Science of the total environment journal), 2020
(www.elsevier.com)
Ordo
Family
Genus
: Nidovirales
: Coronaviridae
: 1. Alphacoronavirus
2. Betacoronavirus
3. Gammacoronavirus
4. Deltacoronavirus
Subgen : Sarbecovirus
Spesies : Severe acute respiratory
syndrome-related
coronavirus
The genus coronavirus that
infects of humans
(ALPHACORONAVIRUS
& BETACORONAVIRUS)
Taxonomy of Virus
Seven Human CoVs (HCoVs) Have Been
Confirmed :
1. HCoV-229E (Alphacoronavirus) (1960)
2.HCoV-NL63 (Alphacoronavirus) (2004)
3.HCoV-OC43 (Betacoronavirus) (1890)
4.HCoV-HKU1 (Betacoronavirus) (2005)
5.Middle East Respiratory Syndrome Coronavirus
(MERS-Cov) (Betacoronavirus) (2012)
6.Severe Acute Respiratory Syndrome Coronavirus
(SARS-CoV) (Betacoronavirus) (2002)
7.Severe Acute Respiratory Syndrome
Coronavirus-2 (SARS-CoV-2)
(Betacoronavirus) (2019-Now)

19. Reference : Genotype and Phenotype of COVID-19 (Journal of Microbiology, Immunology, and Infection www.e-jmii.com) 22 March 2020

20. Reference : Molecular immune pathogenesis and diagnosis of COVID-19 (Journal of Pharmaceutical Analysis, 2020) (https://doi.org/10.1016/j.jpha.2020.03.001)

21. Reference : Probable Pangolin Origin of SARS-CoV-2 Associated with the COVID-19 Outbreak (Current Biology journal), 2020 (https://doi.org/10.1016/j.cub.2020.03.022)

22. Reference : Molecular immune pathogenesis and diagnosis of COVID-19 (Journal of Pharmaceutical Analysis, 2020) (https://doi.org/10.1016/j.jpha.2020.03.001)
NON-STRUCTURAL PROTEINS
STRUCTURAL PROTEINS
N

23. SARS-CoV-2 POLYPROTEIN
STRUCTURAL PROTEINS
NON-STRUCTURAL PROTEINS
NON-STRUCTURAL PROTEINS (20 Proteins) STRUCTURAL PROTEINS (4 Proteins)
STRUCTURALPROTEINS
Reference : Genotype and Phenotype of COVID-19 (Journal of Microbiology, Immunology, and Infection www.e-jmii.com) 22 March 2020
ANTIGEN / NUCLEIC ACID

26. Reference : Genomic characterization of a novel SARS-CoV-2 (Journal Elsevier Inc, April 2020) (https://doi.org/10.1016/j.genrep.2020.100682)

28. Reference : Buku Genetika Manusia, Universitas Gajah Mada, 2016

29. Reference : Insights into the Recent 2019 Novel Coronavirus (SARS-CoV-2) in Light of Past Human Coronavirus Outbreaks (Pathogens 2020,9,186;doi:10.3390/pathogens9030186),
(www.mdpi.com/journal/pathogens)
March, 2020
Reference : Immune responses in COVID-19 and potential vaccines : Lessons learned from SARS and MERS epidemic (Asian Pac J Allergy Immunol DOI 10.12932/AP-200220-0772), 2020

30. Angiotensin Converting Enzyme-2 (ACE-2) and Enzim
Transmembrane Protease serine-2 (TMPRSS2)
Reference : Immune responses in COVID-19 and potential vaccines : Lessons learned from SARS and MERS epidemic (Asian Pac J Allergy Immunol DOI 10.12932/AP-200220-0772), 2020

31. Angiotensin Converting Enzyme-2 (ACE-2) merupakan enzim yang
menempel pada permukaan luar (membran) sel, ditemukan pada
83 % sel epitel alveolus tipe II (paru-paru), sel epitel usus/enterosit,
jantung, ginjal, otot polos, dan sel endotelium (arteri danvena)
Angiotensin Converting Enzyme-2 (ACE-2) terdapat pula pada hati,
korteks serebral, hipotalamus, striatum, dan batangotak
Reference : COVID-19 : A promising cure for the global panic (Science of the total environment journal), 2020 (www.elsevier.com)

32. The Life Cycle of SARS-COV-2
Reference : COVID-19 : A promising cure for the global panic (Science of the total environment journal), 2020 (www.elsevier.com)

33. Reference : Morphology, Genome Organization,Replication, and Pathogenesis of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) (Centre for Advanced Research (CFAR)-Stem
Cell/Cell Culture Unit, Faculty of Medicine, King George’s Medical University (KGMU), Lucknow, India), 2020 (https://doi.org/10.1007/978-981-15-4814-7_3)
TMPRSS2
RIBOSOM
RETIKULUM
ENDOPLASMA
BADAN GOLGI

35. Sumber : Imunologi Dasar Edisi ke-11 (Cetakan Ke-2) Karnen Garna Baratawidjaja & Iris Rengganis, FKUI, 2014

36. Antibodi mempunyai 2 fragmen :
Fragmen antigen binding (Fab) :
mengenal, mengikat Ag Spesifik
dan tempat melekatnya Ag Ab
yang tepat sesuai regio yg
bervariasi
Fragmen cristalizable (Fc) : tidak
dapat mengikat Ag dan
berfungsi sbg efektor yang dapat
berinteraksi dengan sel imun.
STRUKTUR ANTIBODI

37. ANTIGEN-ANTIBODI

38. IgG : terbanyak dalam serum (75%) dapat menembus plasenta
membentuk imunitas bayi, mempunyai sifat opsonin
berhubungan erat dengan fagosit.
IgE : jumlah paling sedikit dalam serum mudah diikat oleh sel
mastosit, basofil dan eosinofil. Kadar tinggi pada kasus alergi,
infeksi cacing.
IgD : sedikit ditemukan dalam sirkulasi, tidak dapat mengikat
komplemen, mempunyai aktifitas antibodi terhadap makanan
dan autoantigen
IgM : tidak dapat menembus plasenta dibentuk pertama kali
oleh tubuh akibat dari rangsangan antigen berfungsi untuk
mencegah gerakan mikroorganisme antigen memudahkan
fagositosis dan aglutinosis kuat terhadap antigen
Ig A : sedikit dalam serum banyak terdapat dalam saluran nafas,
cerna, kemih, air mata, keringat, ludah dan air susu. Fungsinya
menetralkan toksin dan virus.
MACAM-MACAM IMUNOGLOBULIN

39. Reference : Molecular immune pathogenesis and diagnosis of COVID-19 (Journal of Pharmaceutical Analysis, 2020) (https://doi.org/10.1016/j.jpha.2020.03.001)
Reference : Immune responses in COVID-19 and potential vaccines : Lessons learned from SARS and MERS epidemic (Asian Pac J Allergy Immunol DOI 10.12932/AP-200220-0772),2020
Innate Immunity
Adaptive Immunity

40. Reference : Host Immune Response and Immunobiology of Human SARS-CoV-2 Infection (Centre for Advanced Research (CFAR)-Stem Cell/Cell Culture Unit, Faculty of Medicine, King
George’s Medical University (KGMU), Lucknow, India), 2020 (https://doi.org/10.1007/978-981-15-4814-7_5)
NS 15
NS 15
NS 16
NS 1
DMV
NS 3
NS 4
NS 6

41. Reference : GeneTex : A Review of the SARS-CoV-2 (COVID-19) Genome and Proteome, 21 April 2020

42. Reference : GeneTex : A Review of the SARS-CoV-2 (COVID-19) Genome and Proteome, 21 April 2020

43. Reference : GeneTex : A Review of the SARS-CoV-2 (COVID-19) Genome and Proteome, 21 April 2020

46. Do Your best
at every
opportunity
that you
have
(LAKUKAN YANG TERBAIK DISETIAP KESEMPATAN YANG KAMU MILIKI)