A- Imagine you were recently hired as a general manager working in a.docx
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A. Imagine you were recently hired as a general manager working in a company that has never heard about Corporate Social Responsibility (CSR). Make the case to the owners of the business why incorporating CSR into a business us a good idea. B. State two to three reasons why some businesses may not want to adopt CSR. .
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a) Describe two ways the researcher could minimise experimenter bias in this study.
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Nuclear import of SARS-CoV-2 nucleocapsid (N) protein is not inhibited by overmectin B.A. Loney* and M.A. Larkey* *Bundoora Institute for Applied Medical Research. Introduction The causative agent of the current COVID-19 pandemic, SARS-CoV-2, is a single stranded positive sense RNA virus that is closely related to severe acute respiratory syndrome coronavirus (SARS-CoV). It has been previously shown that SARS-CoV-2 nucleocapsid (N) is present in the cytoplasm but can also actively localize to the nucleolus where it can interact with host proteins and also bind to viral RNA. It has also been previously shown that nuclear import of similar nucleocapsid proteins (including the HIV-1 nucleocapsid protein, NC) from other RNA viruses can be inhibited by the drug overmectin resulting in decreased viral replication efficiency. There are multiple nuclear import pathways mediated by different receptors. The two most common nuclear import pathways are mediated by the importin / heterodimer or by the importin homodimer. Overmectin has previously been demonstrated to inhibit nuclear import by disrupting the importin / pathway. In this study SARS-CoV-2 nucleocapsid (N) was transfected into Hela cells and the effectiveness of overmectin at inhibiting its nuclear import was determined. Methods Expression of N protein in the absence of other viral proteins. To investigate the nuclear import of SARS-CoV-2 N protein three constructs were created. 1. pEGFP-NCov2. The N gene (from SARS-CoV-2, isolate BJ04) was cloned into the eukaryotic expression vector pEGFP-C1 (Promega) such that expression of the N gene was under the control of a cytomegalovirus (CMV) polymerase Il promoter to express an N protein fusion with the C-terminal of EGFP. 2. pEGFP. The pEGFP-C1 expression vector alone. 3. EEGFP-TRF1. A positive control for nuclear import. The human TRF1 gene was cloned into the eukaryotic expression vector pEGFP-C1 to express an N protein fusion with the C-terminal of EGFP. TRF1 nuclear localisation has shown to be mediated by the importin homodimer. Hela cells were cultured in 12 separate culture plates at a density of 1 0 5 cells per 9.6 cm 2 plate with each plate containing 2 coverslips. Cells were cultured using Cell Biologics' Culture Complete Growth Medium with 5\% foetal calf serum at 3 7 C and 5% CO 2 . Cells were transfected with 2 g of either pEGFPNCov2 (plates 1,4,7 and 10), pEGFP (plates 2, 5, 8 and 11) or pEGFP-TRF1 (3, 6, 9 and 12) and 50 g of Lipofectamine (GibcoBRL). 12 hours post-transfection, even numbered plates were treated with 5 M overmectin in DMSO and odd numbered plates were left untreated. After 24 hours coverslips were removed, and the cells fixed. DAPI was added to visualise the nuclei and the localisation of GFP determined by fluorescent mic.
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After reading the article on Big Bottom Market make an analysis of the cash flow issues for this business along with any additional observations and your recommendations for action.
Provide your answer as three or four paragraphs. You do not need to respond to the posts of other students as you will be graded on your observations and recommendations for action. Be sure that your recommendations have an IMMEDIATE impact on cash flow, either by preserving cash or generating cash. The facility has a long term lease.
Michael Volpatt, one of the three founders of the Big Bottom Market in Guerneville, Calif. Credit Jim Wilson/The New York Times Big Bottom Market is a year-old restaurant and specialty food store in Guerneville, Calif., that also sells wine and locally made crafts. It is a moonlighting venture for its three owners, among them a San Francisco public relations executive, Michael Volpatt, who saw an opportunity to fill a local need after buying a weekend home in this Sonoma County town of 6,500 . THE CHALLENGE How to survive the lean months, October through April, when visitors to the Russian River getaway dwindle. After opening in July 2011, the market posted encouraging weekly sales of $20 , 000 to $24 , 000 in August - that plummeted to as low as $4 , 000 by November, prompting staff layoffs and an emergency retreat to assess options. THE BACKGROUND Mr. Volpatt, who describes himself as a "serious foodie and serious oenophile," had long dreamed of opening a specialty food and wine store. He decided to go ahead after settling deeper into the community of Guerneville, a oncebooming logging town formerly known as Big Bottom for its location on the alluvial flats of the Russian River. Nowadays, the gay-friendly town is popular with weekenders and vacationers visiting nearby vineyards, kayaking on the river or hiking in nearby Armstrong Woods in what remains of the old-growth redwoods. Mr. Volpatt, 40, would keep his lucrative day job: he owns half of a 10-year-old bicoastal public relations firm, Larkin/Volpatt Communications, serving clients such as the digital divisions of Publishers Clearing House and Hearst. Mr. Volpatt and his business partner, Kate Larkin, 45, who is based in New York, had already invested some of their earnings in real estate, including part ownership in an apartment building in St. Louis. Both felt a market/breakfast and lunch spot was just what Guerneville needed. Chasing that opportunity was a leap into the unfamiliar world of retailing and restaurants but one they cushioned by entrusting the kitchen and market operations to a third founding partner with food and hospitality experience. Mr. Volpatt had become friends with Crista Luedtke, a San Francisco mortgage broker and hands-on owner of Boon Hotel and Spa and Boon Eat and Drink, two popular Guerneville businesses. With Ms. Luedtke overseeing day-to-day operations, Ms. Larkin acting as chief financial officer and Mr. Volpatt in charge of marketing, Big Botto.
A vaccine against HIV Group of answer choices may be impossible due.docx
A vaccine against HIV
Group of answer choices
may be impossible due to the variability and diversity of HIV.
is available for those who can afford it.
prevents opportunistic infections.
consists of cocktails of drugs taken daily.
.
Additional information for the Income Statement- 1 mterest expense of.docx
Additional information for the Income Statement: 1 mterest expense of $12 , 000 is expected to be paid in 2022. 2. Tax rate is 30%
.
Addison Ltd owns all of the share capital of Erin Ltd- In relation to.docx
Addison Ltd owns all of the share capital of Erin Ltd. In relation to the following intragroup transactions, all parts of which are independent unless specified, prepare the consolidation worksheet adjusting entries for preparation of the consolidat ed financial statements as at 3 1 December 2014. Tax rate is 30%.
.
actually I need answers of all these questions as soon as possible ple.docx
actually I need answers of all these questions as soon as possible please answers my all questions.
1. What is hegemony in the political-economic sense? How is Antonio Gramsci connected with this concept? 2. How does the concept of "social class" help to explain labour exploitation? Use examples in Canada and the world. 3. How are the working class and people on the margins of society often portrayed in the mainstream media, such as radio, TV and newspapers? What do the owners of the media gain by portraying people in this way? 4. What is 'broad'casting and what is 'narrow'casting. Give two examples for each (4 in total). 5. You're a consumer of products. What is one negative associated with being someone who is narrowcasted to? Rubric:
.
accumulated depreciation A- is allocated as an expense during future p.docx
accumulated depreciation
A. is allocated as an expense during future periods
B. is reported on the income statement with the expense accounts
C. decreases when the monthly adjustment for depreciation is recognized
D. increases when the monthly adjustment for depreciation is recognized
.
Accounting and Economic profiss are computed differently and serve dif.docx
Accounting and Economic profiss are computed differently and serve different pumposes In defining costs, economists recognize: Explicit and implict costs in the same manner as accountants recognize costs, due to Sarbanes-Oxley act and other accounting iaws Explict and implici costs while accountants recognize only explicit costs Only explicit costs white acoountants recognize only implicit costs Only explicit costs while accountants recognize explicit and implicit costs Only implicit costs while accountants recognize only explicit costs
.
According to the Solow model- Suppose that an earthquake destroys most.docx
According to the Solow model. Suppose that an earthquake destroys most of the capital stock and everything else remains the same.
1,What will happen in the short term? (Demonstrate the adjustment process in the diagram of the Solow model.)
2, What will happen to each worker in the short term?
.
According to Culturally-Endorsed Leadership Theory- cultures differ on.docx
According to Culturally-Endorsed Leadership Theory, cultures differ on what characteristics they believe are essential for a leader to exhibit. Which quality do all cultures universally agree INHIBITS a leader from being effective?
Group of answer choices
Trustworthy
Decisive
Positive
Egocentric
.
According to a study done by Nick Wilson of Otago University Wellingto.docx
According to a study done by Nick Wilson of Otago University Wellington, the probability a randomly selected individual will not cover his or her mouth when sneezing sench in a mall and observe people's habits as they sneeze. Complete parts (a) through (c). (a) What is the probability that among 10 randomly observed individuals, exactly 5 do not cover their mouth when sneezing? Using the binomial distribution, the probability is (Round to four decimal places as needed.)
.
A-) What is the difference between encrypting and signing in asymmetri.docx
A.) What is the difference between encrypting and signing in asymmetric cryptography?
B.) Take a look at the figure below:
Explain what is happening in this specific cryptosystem. Do you believe this is a good model for secure communication? Explain why or why not.
.
a- Write a program to convert an infix expression that includes (- )-.docx
a. Write a program to convert an infix expression that includes (, ), +, -, *, and / to postfix.
b. Add the exponentiation operator to your repertoire.
c. Write a program to convert a postfix expression to infix.
Show the program with a (.cc, .h) files, a makefile that compiles the code, and a readme.txt file that explains how to compile and run the program.
.
A- 1hecrease by atin fi 6- Rawmie tr anan C and I- e- decriate ty arti.docx
This document discusses increasing and decreasing various items but is difficult to understand due to unclear language and lack of context. It mentions rawmie, athan, wein, and ne but does not provide enough information to summarize the key points.
A total of 104 students from Highpool elementary school went on a summ.docx
A total of 104 students from Highpool elementary school went on a summer camp. A chicken salad contaminated with Salmonella was served to all of these students yet only half of them consumed it. 2 days later, 15 of those who ate the salad developed severe symptoms such as diarrhea, fever, and vomiting. After returning to their homes, these ill children infected their family members bringing the total number of cases to 45. The households were comprised of a total of 209 individuals. The city where they resided had a total population of 200,159 individuals (6 pts). a. Compute the primary attack rate b. Calculate the secondary attack rate. c. What is the length of the incubation period?
.
A- Parts of Solutions 1- The substance that exists in the greatest qua.docx
A. Parts of Solutions 1. The substance that exists in the greatest quantity in a solution is called the 2. The substances that exist in lesser quantities in a solution are B. Types of Solutions 1. can exist in three different of matter-as solids, Hquids, or gases. 2. The state of the determines the state of matter of the solution. C. Water as a Solvent 1. Most of the water on Farth is not water; instead, it contains dissolved 2. Water is a(n) molecule, which means that one end of the molecule has a slightly negative charge and the other end has a slightly charge. D. Like Dissolves L.lke 1. solvents dissolve polar solutes easily. 2. solvents dissolved nonpolar solutes easily. 3. Water molecules are polar, so the ends of the molecules are attracted to the oppositely charged ends of other molecules, which makes it easy for polar solutes to in water. 4. Ionic compounds are formed of alternating positive and negative a. The charged ends of a(n) molecule, such as water, are attracted to the that have the opposite
.
A- The distribution is symmetrical B- None of these apply to this dist.docx
A. The distribution is symmetrical
B. None of these apply to this distribution
C. The distribution is skewed to the right
D. The mean > median for this distribution
E. The median most accurately reflects the center of the distribution
F. The mean and the median both accurately reflect the distribution
G. The mean < median for this distribution
H. The distribution is skewed to the left
I. the mean equals median for this distribution
J. The mean most accurately reflect the center of the distribution
.
A- Most human traits are much more complex than pea plant traits B- Th.docx
A. Most human traits are much more complex than pea plant traits
B. The human genome cannot be sequenced, so we can't tell which alleles are recessive and which are dominant
C. All human genes have a dominant and recessive allele, so it is actually very useful
D. There are no dominant and recessive alleles in human genes
2. A dominant allele has an _ chance of being passed down to offspring compared to a recessive allele?
A. Greater
B: smaller
C. Equal
3. If both alleles for a given gene are the same, it is called
crossed-over
A. Mendelian
B. heterozygous
C. homozygous
.
A- 2 bur moup roten to 2 beteroxysore 13- 2 himons gotes to theimorepo.docx
A. 2 bur moup roten to 2 beteroxysore 13. 2 himons gotes to theimorepole C. I heienongsotes to l hopporypote (1) None of the above. 23. Mendal's zuccess in formulating his funideminial grinciples of mheritance ean be attribuied. to which of the follewing? A. Mendel focused on the overall appearance of the plan rather thim co indruidual traits. 1.. Mendel focused on individual traits of fie plant ratser than on the overall appearance. C Mendel chase to study complex traits that cemili froin interacboas betwecn molupie genes D. Mendel used an orgatism that grow alowh. Whefiore baviag long gencration times 24. Which of the following traits will show Mendeciaa behavior? A. dominance B. scmidominance C. codominance D. epistasis 25. Which of the following has the information flowi of the central dogma in the correct order? A DNA, translation, RNA, replication, protein B. DNA, transeription. RNA, reptication, protein C. RNA, uranslation, DNA, transeription, protein D. DNA, transcription. RNA, tanslation, protein 26. Hemophilia in humans results from an X-finked rocessive gene: Which of these statements regarding the inheritance of this trait is true? A. Heterozygous females will exhibit a milder form of hemophilia. B. All daughters of an affected mother will have hemophilin . C. Carrier females will always pass the hemophilia allele to their sons: D. All sons of an affected mother will have hemophilia. 27 In the diagram below, which A. ted arrow indicales the process of 8. recow transistion? c. green D. blue f. burpit 28. Which of the below is incorrect. A. DNA is composed of a 6 carbon sugar B. DNA has a phosphoric acid C. DNA is composed of 4 nitrogen containing bases. D. None of the above
.
A- Identify 7 key brain regions involved in descending modulation of t.docx
A. Identify 7 key brain regions involved in descending modulation of the sympathetic drive during emotional stress, beginning in the hypothalamus (front of the brain) and identify whether you think these regions are excitatory (ie., when activated increase sympathetic drive) or inhibitory (decrease sympathetic drive when activated). B. If there is also an associated change in respiratory activity that parallels a stress related change in sympathetic drive, describe potential pathways activated to trigger a simultaneous change in respiration?
.
A+BC-DEA+BC-D.docx
This mathematical expression contains variables and operators. It involves adding variables A and B, then dividing that result by variables C over D. The same expression is then repeated on the second line.
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