This document lists 50 peer-reviewed publications authored by A.I. Gusev and colleagues between 1991-2006. The publications cover research using time-of-flight mass spectrometry (TOF-MS) and matrix-assisted laser desorption/ionization (MALDI) for applications including quantitative analysis of peptides, proteins, polymers, and other molecules. Many of the publications describe improvements to MALDI and TOF-MS techniques to enhance signal reproducibility, reduce matrix effects, and enable quantification.

1. LIST OF PEER-REVIEWED PUBLICATIONS
1. A.I. Gusev; V.A. Kochnev and G.G. Managadze "Investigation of Noise Background Parameters of
Time-of-Flight Mass Spectrometer MARSION", Published by Institute of Space Research, Moscow,
Pr.-1774, 1991, 28.
2. A.I. Gusev; V.A Kochnev and G.G. Managadze "Time-of-Flight Mass Spectrometer with Increased
Area of Ion Gathering and Large Geometrical Factor", Published by Institute of Space Research, Pr.-
1780, Moscow, 1991, 26.
3. A.I. Gusev; V.A. Kochnev; G.G. Managadze" Formation of Neural Particle Peaks in Time-of-Flight
Reflectron", Pis'ma v JTF.(Soviet Let. JTF), 1991, 17, 44.
4. N.Y. Vedenov and A.I. Gusev "Space-Time Focusing in the Ion Sources of Time-of-Flight Mass
Spectrometers", Published by Institute of Space Research, Pr.-1782, Moscow, 1991, 14.
5. A.I. Gusev; G.G. Managadze and I.Y. Shutyaev "Improving of Relative Sensitivity of Mass
Reflectron by Averaging of Mass-Spectra", Pis'ma v JTF. (Soviet Let. JTF), 1992, 18, 63.
6. A.I. Gusev; W.R.Wilkinson; A.Proctor and D.M. Hercules "Quantitative Analysis of Peptides by
Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry", Applied Spectrosc.,
1993, 47, 1091.
7. D.C.Muddiman; A.I. Gusev; A.Proctor and D.M. Hercules, R.Venkataramanan and W.Diven,
"Quantitative Measurement of Cyclosporin A in Blood by Time-of-Flight Mass Spectrometry",
Anal.Chem., 1994, 66, 2362.
8. A.I. Gusev; W.R.Wilkinson; A.Proctor and D.M. Hercules "Improvement of Signal Reproducibility
and Matrix/Comatrix Effects in MALDI Analysis", Anal.Chem., 1995, 67, 1034.
9. A.I. Gusev; A.Proctor; Y.I Rabinovich and D.M. Hercules, "Thin Layer Chromatography Combined
with Matrix Assisted Laser Desorption/Ionization Mass Spectrometry", Anal.Chem. 1995, 67, 1805.
10. D.C.Muddiman; A.I. Gusev; K.Stoppek-Langner; A.Proctor; D.M. Hercules; P.Tata;
R.Venkataramanan and W.Diven, "Simultaneous Quantification of Cyclosporin A and its Major
Metabolites by TOF-SIMS and MALDI/TOF-MS Utilizing Data Analysis Techniques: Comparison
with HPLC", Journal of Mass Spectrom., 1995, 30, 1469.
11. A.I. Gusev; O.L.Vasseur; A.Proctor; A.G.Sharkey and D.M. Hercules, "Imaging of Thin Layer
Chromatograms using Matrix Assisted Laser Desorption/Ionization Mass Spectrometry", Anal.Chem.
1995, 67, 4565.
12. A.J.Nicola, A.I. Gusev; A.Proctor; E.K.Jackson and D.M. Hercules, "Application of the Fast
Evaporation Sample Preparation Method for Improving Quantification of Angiotensin II by MALDI",
Rapid Comm. Mass Spectrom., 1995, 9, 1164.
13. D.M. Hercules; A.I. Gusev; A.Proctor and D.C.Muddiman, "Quantitative Analysis of Organics using
TOF-SIMS", SIMS X, 1995, ed. by Benninghoven, A., Wiley, Chichester, UK.
14. D.C.Muddiman; A.I. Gusev; L.M. Martin and D.M. Hercules, "Direct Quantification of Cocaine in
Urine by Time-of-Flight Mass Spectrometry", Fresenius' J. of Anal. Chem., 1996, 354, 103.
15. A.I. Gusev; D.C.Muddiman; A.Proctor; A.G.Sharkey; D.M. Hercules; P.Tata and
R.Venkataramanan, "A Quantitative Study of in vitro Hepatic Metabolism of Tacrolimus (FK506)
using SIMS and MALDI", Rapid Comm. Mass Spectrom., 1996, 10, 1215.
16. J.B.Williams; A.I.Gusev and D.M.Hercules , "Use of Liquid Matrices for Matrix Assisted Laser
Desorption Ionization of Synthetic Polymers", Macromolecules, 1996, 29, 8144.
17. A.I. Gusev; W.R.Wilkinson; A.Proctor and D.M. Hercules, "Direct Quantitative Analysis of
Peptides and Proteins using MALDI", Fresenius' J. of Anal. Chem. 1996, 354, 455.
18. W.R.Wilkinson; A.I. Gusev; A.Proctor; M.Houalla and D.M. Hercules "Selection of Internal
Standards for Quantitative analysis by MALDI TOF MS", Fresenius' J. of Anal. Chem., 1996, 357,
241.

2. 19. A.J.Nicola; A.I. Gusev and D.M. Hercules, "Development of Quantitative Analysis by Thin Layer
Chromatography Combined with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry",
Applied Spectrosc., 1996, 50, 1479.
20. J.B.Williams; A.I.Gusev and D.M.Hercules, "Characterization of Polyesters by MALDI TOF MS",
Macromolecules, 1997, 30, 3781.
21. T.N.V.Prasad, N.Subbotina, G.Burckart, A.Swaminathan, A.I.Gusev, D.M.Hercules, and
R.Venkataramanan, "Metabolism of Tacrolimus (FK506) in Rat Liver Microsomes: Effect of
Rifampin and Dexamethasone, Res. Comm. Mol. Pathol. and Pharmacol., 1997, 96, 107.
22. J.T.Mehl, A.I. Gusev, D.M.Hercules, ”Coupling Protocol for Thin Layer Chromatography/Matrix-
Assisted Laser Desorption Ionization”, Chromatographia, 1997, 48, 358.
23. A.I. Gusev, B.K.Choi, D.M.Hercules, ”Improvement of Signal Intensities in TOF-SIMS Mass
Spectrometry using Halide Additives and Substrate”, Journal of Mass Spectrom, 1998, 33, 480-485.
24. J.T.Mehl, A.I. Gusev, D.M.Hercules, “Direct Coupling of TLC with MALDI Mass Spectrometry”,
American Laboratory, 1998, 30, 30.
25. A.J.Nicola; A.I. Gusev, X.Gao, K.L. Brigham, D.M. Hercules ”Analysis of Cationic Lipids Used in
Gene Therapy by Static Secondary Ion MS ", in SIMS XI, p.97-100, 1998., Wiley, Chichester, UK.
26. A.I. Gusev, B.K.Choi, D.M.Hercules, ”Improvement of Signal Intensities by Halide Surface
Modifications TOF-SIMS Mass Spectrometry using Halide Additives and Substrate", SIMS XI,
p.575-577, 1998,., Wiley, Chichester, UK.
27. X.Dong, A.I. Gusev, D.M. Hercules, “Characterization of Polysiloxanes with Different Functional
Groups by TOF-SIMS”, Journal of the American Society for Mass Spectrom. 1998, 9, 292.
28. A.J.Nicola; A.I. Gusev and D.M. Hercules “Automation of Data Collection for MALDI Mass
Spectrometry using a Correlative Analysis Algorithm", Anal. Chem., 1998, 70, 3213.
29. S.Reddy, X.Dong, R.Murgasova, A.I. Gusev, D.M. Hercules, “Synthesis and Secondary-Ion Mass
Spectrometry of Linear Single Oligomers of Nylon-6”, Macromolecule, 1999, 32, 1367.
30. R.Vermillion-Salsbury, A.A.Hoops, A.I. Gusev and D.M. Hercules “Analysis of Cationic Pesticides
by TLC/MALDI”, International J. of Environmental. Anal. Chem., 1999, 73, 179-190.
31. K.Xu, A.I.Gusev and D.M.Hercules, "Stereoregular Polypropylens Studied by Time-of-Flight Mass
Spectrometry and Atomic Force Microscopy", Surface and Interface Analysis, 1999, 27, 659-669.
32. D.T.Isbell, A.I. Gusev, N.I.Taranenko, C.H.Chen, D.M.Hercules “Separation and Detection of
Carcinogen-Adducted Oligonucleotides”, J. Mass Spectrom, 1999, 34, 774-776.
33. B.K.Choi, A.I. Gusev, D.M.Hercules “Postcolumn Introduction of an Internal Standard for
Quantitative LC/MS Analysis”, Anal.Chem. 1999, 71, 4107.
34. D.T.Isbell, A.I. Gusev, N.I.Taranenko, C.H.Chen, D.M.Hercules “Analysis of nucleotides directly
from TLC plates using MALDI MS detection”, Fresenius' J. of Anal. Chem., 1999, 365, 625.
35. Q.Zhang, A.I.Gusev, D.M. Hercules “A Novel On-line Coupling of Liquid Chromatography with
MALDI Mass Spectrometry Detection”, Rapid Comm. Mass Spectrom., 1999, 13, 2278.
36. B.K.Choi, A.I. Gusev, D.M.Hercules “Quantitative Analysis for Pesticides in Complex
Environmental Matrices using External and Internal Standards”, International J. of Environ. Anal.
Chem., 2000, 77, 305.
37. B.K.Choi, A.I. Gusev, D.M.Hercules, ”Investigation of Static-SIMS Signal Improvement by Halide
Surface Substrate Treatment”, Surface and Interface Anal.., 2000, 29, 284-291.
38. B. K. Choi, D. M. Hercules, E. Michelotti, B. Martinez, M. Eisenschmied, T. Zhang, A. I. Gusev
“Application of VB for Automation of LC-MS ”, J.of Ass.for Lab. Automation, 2000, 5, 102-105.
39. B. K. Choi, D. M. Hercules and A. I. Gusev “
The Effect of LC Separation of Complex Matrices on
LC-MS/MS Signal Suppression”, Journal Of Chormatography A, 2001, 907, 337-342.

3. 40. B. K. Choi, D. M. Hercules, T. Zhang, A. I. Gusev, “Comparison of Quadrupole, Time-of-Flight,
and Fourier Transform Mass Analyzers for Qualitative and Quantitative LC-MS Applications”, LC-
GC, 2001, 19, 514-524.
41. B. K. Choi, D. M. Hercules, A. I. Gusev “LC-MS Signal Suppression Effects in Complex Matrices”,
Fresenius' J. of Anal. Chem., 2001, 369, 370-377.
42. M.J.Mulvihill, D.V.Nguyen, B.MacDougall, B.Martinez-Teipel, R.Joseph, J.Gallagher, D. Weaver,
A.I. Gusev, K.-H. Chung, W.Mathis “Benzaldehyde-derived chloroformates and their application
towards the synthesis of methoxyfenozide-N-[(acyloxy)benzyloxy]carbonyl derivatives”, Tetrahedron
Lett., 2001, 42, 7751-7754.
43. R.Pascoe, J.P Foley, A. I. Gusev “Development and Application of 2-Dimensional Chromatography
Combined with Electrospray Mass Spectrometry for Accurate Qualitative and Quantitative Analysis”,
Anal. Chem, 2001, 73, 6014-6023.
44. M.J.Mulvihill, S.H Shaber, B.MacDougall, C. Ajello, B.Martinez-Teipel, R.Joseph, D.V.Nguyen, D.
Weaver, A.I. Gusev, K.-H. Chung, , J.Wieremga, W.Mathis “Synthesis of Active Halofenoze-
[(acyloxy)alkoxy]carbonyl and (acyloxy)alkylcarbonyl”, Synthesis., 2002 (2002), (1), 53-58
45. B. K. Choi, D.M.Hercules, N.Sepetov, O. Issakova, A. I. Gusev “Intelligent Automation of LC-MS
for Analytical Screening of Compound Libraries Using VB Macros”, LC-GC, 2002, 20 (2), 152.
46. L.H.Cohen and A.I.Gusev “Small Molecule Analysis by MALDI Mass Spectrometry”, Analytical and
Bioanalytical Chemistry, 2002, 373, 571-586.
47. B. K. Choi, D. M. Hercules, T. Zhang, A. I. Gusev, “Comparison of Quadrupole, Time-of-Flight,
and Fourier Transform Mass Analyzers for Qualitative and Quantitative LC-MS Applications”,
Spectroscopy, 2003, 18 (5s), S24-S31.
48. Y. Alnouti, K. Srinivasan, D.Waddell, H.Bi, O. Kavetskaia, and A. I. Gusev “Development and
Application of a New on-line SPE System Combined with LC-MS/MS Detection for High Throughput
Direct Analysis of Pharmaceutical Compounds in Plasma”, Journal of Chromatography A, 2005,
1080, 99-106.
49. M. Li, Y. Alnouti, R. Leverence, H.Bi, A.I. Gusev “Increase of the LC-MS/MS Sensitivity and
Detection Limits Using On-line Sample Preparation with Large Volume Plasma Injection”, Journal of
Chromatography B, 2005, 825,152–160
50. Y. Alnouti, M.Li, O. Kavetskaia, , H.Bi, C. Hop and A. I. Gusev, “A New Method for Internal
Standard Introduction for Quantitative Analysis using On-line SPE LC-MS/MS , Anal. Chem., 2006,
78(4), 1331-1336.
51. R. Leverence, M.Avery, O. Kavetskaia, , H.Bi, C.E.C.A Hop and A. I. Gusev, “Signal
Suppression/Enhancement in HPLC-ESI-MS/MS from Concomitant Medications" Biomedical
Chromatography , 2007, 21: 1143–1150.
52. A. I. Gusev and C.E.C.A Hop “LC-MS(MS) maintenance, service and support", Managing the
Modern Laboratory, 2008, 9: 19-22,
53. M.Li, H Xu, T.Freeman, B. Kuang, H. Wang and A.I. Gusev “An Automated Bioanalytical Standards
and QC Sample Preparation Method” J.of Association for Lab.Automation,2008 (submitted)
INVITED PAPERS (1995 - present)
54. D.C.Muddiman; A.I. Gusev and D.M. Hercules, "Application of Secondary Ion and Matrix Assisted
Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for the Quantitative Analysis of
Biological Molecules", Mass Spectrometry Reviews, 1995, 14, 383.
55. A.I.Gusev ”Interfacing of MALDI Mass Spectrometry with Column and Planar Separations”,
Fresenius' J. of Anal. Chem., 2000, 366, 691
PATENTS (1995 - present)

4. 56. A.I. Gusev; D.M.Hercules, "On-line Coupling of Liquid Capillary Separation with Solid-State Mass
Spectrometry Detection ", US 6,140,639 May 29, 1998 (2000).
SELECTED RESENTATIONS
o J. F. Banks; E.Soderstrom; D.Tess; M.Bourgeois; A.I Gusev “Enhancing Effectiveness For LC/MS
Pharmaceutical Laboratory Analysis Through Rational Process Mapping”, Proceedings of the 51-
th ASMS Conference on Mass Spectrometry and Allied Topics, Montreal, Canada, 2003
o Y. Zhang, J. Hollembaek, A.I Gusev, D. Tang, L. Cohen, S. Michael “Normal Phase HPLC-ESI-
MS-MS Quantitative Analysis for Chiral Compounds in Biological Matrices” Proceedings of the 52-
th ASMS Conference on Mass Spectrometry and Allied Topics, Nashville TN, 2004.
o A.I. Gusev, et al., “Towards High Throughput Bioanalytical Support”, NJ MSDG, 2004,
o A.I. Gusev, M. Li, “Increasing Speed, Sensitivity, and Selectivity for Clinical Bioanalysis”,
Bioanalysis in Clinical Research, London UK, 2008
o M Li, T. Freeman, B.Kuang, H. Wang, A. Gusev, Semi-Automatic ELISA Assay On Hamilton
MicroLab STAR”, Lab Automation 2008, Palm Springs CA, 2008
o D. He, D. Tess, N.Boone, R. Scialis, T. Archer, and A.I.Gusev “e-Lab Notebook Application in
ADME Studies: From Data to Knowledge”, CPSA, 2008.