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The document discusses the history and development of chocolate over centuries. It details how cocoa beans were first used by Mesoamerican cultures before being introduced to Europe. Chocolate then evolved from a luxury good to a mass-produced confection as production methods advanced and it became commercially viable on a larger scale.

Biological screening of herbal drugs: Introduction and Need for Phyto-Pharmacological Screening, New Strategies for evaluating Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and Antifertility, Toxicity studies as per OECD guidelines

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