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2. Given the following manipulations, how would you expect them to affect sea urchin
development? Be as specific as possible.
A. Rather than allowing for natural fertilization, inject the sperm into the animal half of the oocyte.
B. Grow the tunicate embryos in sea water containing Brachyury protein.
C. Remove the vegetal cells from a tunicate blastula and replace them with animal pole cells from
another blastula.

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