This document discusses various uses of probiotics in different areas of health. It summarizes that probiotics are live microorganisms that have proven beneficial effects on the host's body when given in sufficient amounts. Some key points made include that certain probiotics like Lactobacillus rhamnosus GG and Saccharomyces boulardii can shorten the duration of acute gastroenteritis by 1 day. Probiotics may also help lower bacterial load of H. pylori but cannot eliminate it completely. Probiotics are also discussed in relation to skin health conditions like acne, bone health, urinary tract infections, and C. difficile treatment.

1. Silvia Gerčáková, Andrej Sipták

2.  The definition
 Utilisation – gastroenterology
Probiotics and H.pylori
Probiotics and metabolic diseases
 Utilisation – dermatology
 Probiotics and bone metabolism
 Probiotics in UGT infections treatment
 Traspoosion

3.  Probiotics
Live microorganisms, which, given in sufficient
amount, have proveably benefitial effect on the
host`s organism
 Prebiotics (fructooligosacharids, inulin, lactulose)
nutritional substrates of probiotic cultures, the
product of their catalysis are substances supporting
the nutrition of the bowels
 Synbiotics = prebiotic + probiotic
(oligosacharids + Bifidobacteriae )

4.  There were 12 guidelines compared in the pattern of acute gastroenteritis
treatment, 5 of them recommend the probiotics use, the other 7 do not.
Those, which supported the use of antibiotics were additionally proved by the
results of clinical studies.
 The effect is significant in treatment of virus diarrhea, but not in bacterial
diarrhea.
 The effect is related to the bacterial tribe, the dose and time of the initiation
of the treatment
 Prevention: traveler`s diarrhea
Clostridium difficile diarrhea
Nectrotising enterocolitic
The most significant effect had Lactobacillus rhamnosus GG (LGG) and
Saccharomyces boulardii, which shortened the duration of the disease by 1 day.

6.  The results of double blind placebo
controlled studies prove that specific
probiotics like S. boulardii and L.
johnsonni LA1 are able to lower
bacterial load , but are not able to
eliminate H.pylori completely
 Probiotics can lower the presence of
side effects of antibiotics treatment and
in this way speed up the eradication of
HP

7. -Dysmicrobia can possibly initiate the autoimune proceses
-One of possible triggers of DM1 is dysmicrobia
-Providing the probiotic treatment to a genetically vulnerable
newborn is connected to lower the risk of DM1 development
in 60%
-This effect was not proved in children without any genetic
predisposition.

8.  acne and rosacea
 Local application of probiotics has cytocidal effect on
harming – inflammation causing microorganism and so
leads to suppression of the inflammation.
 Oral probiotics containing Lactobacilli and/or
Bifidobacteria can affect the process of acne or rosacea
through the GIT – brain – skin axis.
According to this theory, stress alone or with
combination with unhealthy fibre poor diet slows down
the peristaltics. This change the composition of PMF
(physiological microflora). It influences the permeability
of mucous barriere, causes toxemia and toxemia
supports the exhibition of skin inflamatory signs.
 Recent korean study with 56 patients with acne
confirmed that drinking of the liquids containing
Lactobacilli fr 12 weeks, effectively reduce sebum
production

9. How does it work:
-support the solubility of minerals due to production of fatty
acids with short chain
-enlarge the absorption surface of the bowel – they support
enetrocytes proliferation through the production of lactate and
other compounds
-stimulate the expression of Ca2+ binding proteins
-stabilisate intestinal mucosa and the composition of PMF
There is a study confirming 36%
higher density of bonesin
menopausal women
with 8 weeks long probiotic treatment.

10.  Probiotics therapy was reviewed in relation to
prevention of urinary tract infections.
 Considering the fact, that Lactobacilli dominate in
urogenital tract microflora of healthy
premenopausal women, the idea of its
supplementation in patogenic conditions was
born.
 There is proved success in therapy of reccurent
urinary tract infections.

11.  90 % success in treatment of C.difficile enteritis even after
unsuccessful therapy with vankomycin
 Since the 1st written mention of FMT in 4th century in
Chine, there has never been any serious side effect
observed!
 It is still considered to be an experimental therapy, not only
of GIT and AI diseases, but also in treatment of
neurological diseases like SM
 OpenBiome, the first public stool bank in the United States