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Introducing NGB-R, a Disruptive 4D Bioprinting System
Join us on February 25, 2021, to learn more about a state-of-the-art, one-of-a-kind, disruptive 4D bioprinting technology from Poietis. Focusing on a cytocentric approach, tissues grow in a more natural environment, leading to proper morphogenesis and predictable biological processes, including cell differentiation, migration, multiplication, and metabolic processes. The NGB-R platform integrates automation and robotics, numerous online sensors, and Artificial Intelligence processing. In addition, it integrates all bioprinting techniques (laser-assisted bioprinting, bioextrusion, and micro-valve bioprinting), a world’s first in the bioprinting market. This is a first of a 3-part series that will provide an introduction and overview to the technology, a survey of various 4D bioprinting applications, and a live hands-on demonstration of the hardware and software.” In this first of three sessions in this series, we will introduce the NGB-R system and delve more into the idea of disruptive 4D bioprinting technology – focusing on the cytocentric approach which allows tissues to grow in a more natural environment, leading to proper morphogenesis and predictable biological processes including cell differentiation, migration, multiplication and metabolic processes. We will review the features and benefits of the system, along with the system components. The NGB-R system has been designed for advanced bioprinting applications, including novel laser-assisted bioprinting technology, along with more conventional extrusion and inkjet techniques. Fabrication plans can include any combination of all three techniques to ensure optimal design and patterning of cells, bioinks, and extracellular matrix compounds REGISTER FOR SESSION #1 REGISTER FOR SESSION #2 REGISTER FOR SESSION #3 This webinar will include a workflow video showing the NGB-R system in action, including the robotic arm, the laser-assisted printing head, as well as the integrated microscope to help visualize the progression of the fabrication process. A few key examples will be shown to highlight full capabilities of the NGB-R system in 4D bioprinting of tissues such as skin. Please make sure to join us for the second session in this series for a more detailed review of this and other successful applications of the NGB-R system, including pancreatic and liver tissues, brain organoids, as well as cartilaginous tissue. In the third and final session in this series a live virtual demo will be provided showing a detailed overview of both the software and hardware components of the NGB-R system.
Introducing NGB-R, a Disruptive 4D Bioprinting System
- 1. Introducing NGB-R, a Disruptive 4D BioprintingSystem A New 4D Bioprinting Approach to make Complex, Functional Living Tissues
- 2. Tonya Coulthard Manager, ImagingDivision Scintica Instrumentation Introducing NGB-R, a Disruptive 4D Bioprinting System A New 4D Bioprinting Approach to make Complex, Functional, Living Tissues
- 3. WWW.SCINTICA.COM Topics of Discussion •New Partnership – Scintica Instrumentation and Poietis • Audience Poll • NGB-R System Overview • Disruptive & Cytocentric Approach to Bioprinting • Advanced Cell Patterning and 4D Bioprinting • Audience Poll • Q & A Session
- 4. WWW.SCINTICA.COM New Partnership February16, 2021 Scintica Instrumentation • Founded in 2017 in London, ON Canada; with offices in USA and Europe • Mission: • Works to identify new and innovative systems, providing scientific support to link researchers with well designed equipment • Exclusive distributor of the NGB-R system in North America Poietis • Founded in 2014 in Bordeaux, France • Mission: • Translational Research – design and make actual, functional tissues not mere 3D scaffolds • Regenerative Medicine – bring new, effective tissue-engineering therapies to patients
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- 6. WWW.SCINTICA.COM NGB-R System Overview (NextGeneration Bioprinting for Research) • All-in-one, standalone, fully integrated, bioprinting platform; including a biosafety cabinet (BSL-2) • High-end robotics and automation tools for unrivaled precision and reproducibility • Powerful onboard computer with touch screen State-of-the-art Hardware
- 7. WWW.SCINTICA.COM Hardware Features andBenefits Fully Integrated Biosafety Cabinet • Saves space and money with an all-in-one cabinet for al your cell and bioprinting work Industry-Grade 6-Axis Robotic Arm • Enjoy unrivaled precision and automation with fully motorized robotics Large 21” Touch-Screen Interface • Operate your bioprinter in optimal conditions with stunning visualization Bio-Extrusion and Micro-Valve Heads • Continue your current bioprinting work and transfer your protocols to NGB-R Exclusive Laser Bioprinting Head • Discover single-cell resolution and keep total control over cell distribution patterns Built-In Cellular-Level Microscope • Get stunning images and monitor your tissues without taking them out of the bio-safety cabinet
- 8. WWW.SCINTICA.COM Software Features andBenefits • User friendly operation Seamless workflow from design on your computer to fabrication on the unit itself. • Intuitive planning tools to create detailed patterns Customize layers & fabrication sequences to meet your needs. • Options to monitor the fabrication process and to perform image analysis. Smart Software Suite
- 9. WWW.SCINTICA.COM The Concept ofCytocentric Bioprinting 1Cytocentric Software Advanced cell patterning, layer-by-layer architecture 2Laser Bioprinting Unrivaled cell-distribution precision, resolution & viability 3 Fully Robotics Operated Operator-independent processes for automation & reproducibility 4 Maturation Monitoring On-board imaging tools for quality-control and monitoring
- 10. WWW.SCINTICA.COM Major Advantages Reproducibility and Automation •Operator independent processing with industry-grade robotics for user- friendly and reproducible workflows Multi-modality and versatility • 3-in-1 instruments to meet most application requirements and make more complex tissue models Resolution and Control • From resolution and volume, to spatial distribution of cells and tissue modeling, laser assisted bioprinting on the NGB-R gives the user ultimate control
- 11. Antoine Jacquot Sales Manager Poietis ADisruptive and Cytocentric Approach to Bioprinting Purpose for biomaterials, laser- assisted bioprinting and precise cell distribution
- 12. Biomaterial purpose Minimizethe negative impact of shear stress and prevent cells from differentiating/dying in the process Biomaterial purpose Maximize tissue maturation and help key biological functions emerge for better results Biomaterial/hydrogel Living cells + Conventional Bioprinting Poietis’ Cytocentric Approach Living cells in culture media or with proper hydrogel Biomaterial/hydrogel serving as extra-cellular matrix Microsion Laser Bioink Bioink Biomaterial Extrusion
- 13. Laser-Assisted Bioprinting Explained Receiver Bioinkjet Cell bioink Donor Focal lens Laser beam Mirror 10,000 droplets per second pL to nL droplet volume 20 to 300 mPa·s viscosity range 1 to 100+ cells per droplet 1 to 100 million cells per mL 10 µm precision
- 14. Cell Distribution WithLaser-Assisted Bioprinting 500µm Laser-assisted bioprinting Laser-bioprinted cell droplets
- 15. Multi-Modal 3-in-1 Bioprinting Laserassisted Droplets – from pL to nL Cells & medium Inkjet/micro-valve Drops – up to 100nL Stable liquid-type biomaterials Bio-extrusion Filaments – up to 100µm Viscous, gel-type biomaterials
- 16. Printing Living Cells& Extra-cellular Matrix Separately Cell droplets Cell droplets ejected and incorporated onto a layer of biomaterial/hydrogel Layer by layer - 2D to 3D to 4D Extra-cellular matrix Primary “bed” of biomaterial ensuring smooth landing and proper cell adhesion
- 17. Key Advantages ofLaser-Assisted Bioprinting 2 Superior Resolution 1 Unrivaled Cell Viability 3 Spatial Cell Distribution
- 18. Key Advantages ofLaser-Assisted Bioprinting Live/dead cell viability tests on cells after laser bioprinted calcein and ethidium stainings 1 Unrivaled Cell Viability Print cells with a nozzle-free technology which does not harm, damage nor injure them throughout the bioprinting process. Primary Human Fibroblasts ~95 % Primary Human Keratinocytes ~95 %
- 19. 140µm 500µm 1 00µm Key Advantages ofLaser-Assisted Bioprinting Examples of different resolution levels using laser-assisted bioprinting to incorporate living cells into a bioprinted tissue 2 Superior Resolution Adjust printing resolution by playing with cell concentration on the donor and with the laser beam energy level. Single cell bioprinting ~70% success Cellular spots pattern ~50 cells/spot Cell aggregate (spheroid) ~300µm Ø
- 20. 500µm 500µm Key Advantagesof Laser-Assisted Bioprinting Comparision between bio-extrusion and laser-assisted bioprinting to incorporate living cells into a bioprinted tissue 3 Spatial Cell Distribution Distribute cells spatially in 3D within the ECM according to specific micro-patterns so as to induce tissue biological functions. Living cells bioprinted through bio-extrusion Random cell distribution Living cells bioprinted through laser-assisted bioprinting Precise cell distribution
- 21. Key Advantages ofLaser-Assisted Bioprinting 3 Spatial Cell Distribution > Tissue Morphogenesis Precise spatial cell distribution across the bioprinted tissue leads to key biological functions emergence and proper tissue morphogenesis
- 22. Mikael Garcia Business ApplicationManager Poietis Advanced Cell Patterning and 4D Bioprinting From 3D to 4D Bioprinting, cell droplets patterning and tissue morphogenesis
- 23. WWW.SCINTICA.COM Designing And TestingCell Patterns Micro-pattern of cell droplets - CAD software - Bioink cell concentration (µL) 10 µl Laser beam power (µJ) 12 µl 8 µl 0-5 microbeads/spot 2 µJ ~20 microbeads/spot 2,5 µJ ~30 microbeads/spot 3 µJ
- 24. Higher quantity ofcells per pattern 4D Bioprinting Through Advanced Cell Patterning Lower quantity of cells per pattern
- 25. WWW.SCINTICA.COM 4D Bioprinting Through AdvancedCell Patterning Multi-layer, cell-droplet pattern tracked over time from day 0 to day 2 and imaged with 3D microscopy
- 26. WWW.SCINTICA.COM 4D Bioprinting Through AdvancedCell Patterning Tracking a cellular-spot pattern over time: Specific pattern of Primary-human Fibroblasts, laser-bioprinted over a layer of Collagen-I Sample - day 0 Sample – hour 18 Sample – day 4
- 27. WWW.SCINTICA.COM Pattern Time Biological functionsemergence Tissue morphogenesis 4D Bioprinting Through Advanced Cell Patterning 500µm + Taking time into account when designing micro-patterns of tissue components to induce key biological functions and optimize the morphogenesis process. TISSUE MODELING CONTROL
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- 29. WWW.SCINTICA.COM Full-skin Tissue Model BiofabricationWorkflow 0 - 5 days Primary fibroblasts C ollagen I Repeat Membrane DERMIS Dermisequivalent Primarykeratinocytes EPIDERMIS Tissue CAD Maturation / air-lift Completion Maturation / immersion Fabrication 1 5 2 3 4 7 - 12days
- 30. WWW.SCINTICA.COM Full-skin Tissue Model BiofabricationWorkflow F1- Fibroblasts 500 µm C2 - Collagen 500 µm F2 - Fibroblasts 500 µm
- 31. WWW.SCINTICA.COM Analyzing post-printing biological functionsemergence with Fibroblast cells No difference between bioprinted cells and controls were observed, proving good bio-compatibility of the process. 4D Bioprinting Through Advanced Cell Patterning
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- 33. WWW.SCINTICA.COM Full-skin Tissue Model BiofabricationWorkflow Poieskin® immunostaining Poieskin® histological section
- 34. WWW.SCINTICA.COM Applications Area European ProjectsIndustrial Partners Brain models & neuronal disorders ASCTN T raining Bioprinted pancreatic tissue units Pan3D Hepatotoxicity testing Servier Hair follicle L’Oréal Active ingredients BASF 3D Bioprinted human liver tissue showing Hepatocyte cells (Green) expressing Nuclear Factor 4, Duct CK19-positive cells, and nuclei (Blue). Customized 3D bioprinted skin tissue
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- 36. WWW.SCINTICA.COM Topics of Discussion •New Partnership – Scintica Instrumentation and Poietis • NGB-R System Overview • Disruptive & Cytocentric Approach to Bioprinting • Advanced Cell Patterning and 4D Bioprinting • Q & A Session
- 37. Questions? Please enter yourquestions in the Q&A Any questions not addressed during the answered following the event. For additional information reach out to visit www.scintica.com Tonya Coulthard Manager, Imaging Division Scintica Instrumentation Antoine Jacquot Sales Manager Poietis Mikael Garcia Business Application Manager Poietis