Hair cloning involves multiplying germinative hair follicle cells outside the body and re-implanting them to grow new hair follicles and permanent hair. Hair multiplication uses donor hair follicles manipulated to increase hair amount. Models for cloning hair include implanting dermal papilla cells alone or with keratinocytes, or alongside miniaturized follicles. Challenges include determining the best follicular components and ensuring cells are successfully cultured, aligned in a matrix, and strongly injected to induce consistent hair growth.

1. Hair Cloning
THE FUTURE OF HAIR RESTORATION
http://www.clinicspots.com/

2. What is Hair Cloning?
• A promising treatment for androgenetic alopecia, or common genetic
hair loss
• Being actively researched by pioneer hair restoration physicians
• In hair cloning, a sample of a person’s germinative hair follicle cells
are multiplied outside the body (in vitro), and then they are re-
implanted into the scalp
• These germinative hair follicle cells grow new hair follicles and
subsequently into new permanent hair.

3. • In contrast to hair cloning, where germinative cells are multiplied outside the
body in essentially unlimited amounts, in hair multiplication, donor hair follicles
are removed from the scalp and then manipulated in a way that the total amount
of hair is increased.
• This can involve using transected, or cut, hair follicles and implanting them
directly into the scalp with the hope that the follicles will regenerate and grow a
complete hair
• The concept behind hair multiplication using plucked hair is that it is an easy, non-
invasive method of obtaining germinative cells
• removing a small proportion of the germinative cells, through plucking, may
provide enough tissue for the formation of a new follicle while not diminishing
the original one.
What is Hair Multiplication?

4. Models for Cloning Hair
Implanting Dermal Papillae cells alone
Placing DP cells alongside miniaturized follicles
Implanting DP cells with keratinocytes
Cell Implantation using a Matrix.

5. • THE DP CELLS COAX THE KERATINOCYTES OF THE REDUCED
FOLLICLES TO DEVELOP INTO TERMINAL HAIRS
• THE EXISTING MINIATURIZED FOLLICLES ALREADY HAVE THE PROPER
STRUCTURE AND ORIENTATION TO PRODUCE A NATURAL LOOK
Implanting Dermal Papillae cells alone

6. Placing DP cells alongside miniaturized follicles
• THE DP CELLS COAX THE KERATINOCYTES OF THE REDUCED
FOLLICLES TO DEVELOP INTO TERMINAL HAIRS
• THE EXISTING MINIATURIZED FOLLICLES ALREADY HAVE THE PROPER
STRUCTURE AND ORIENTATION TO PRODUCE A NATURAL LOOK

7. Implanting DP cells with keratinocytes
• KERATINOCYTES AND DP CELLS ARE GROWN TOGETHER UNTIL THERE
IS PARTIAL HAIR FORMATION
• THE CULTURE-GROWN HAIRS (“PROTO-HAIRS”) ARE THEN IMPLANTED
IN THE SKIN
• WE GET BETTER HAIR DIRECTION BECAUSE OF THE ORIENTATION OF
THE PROTO-HAIR

8. Cell Implantation using a Matrix.
• DP CELLS ALONE OR IN COMBINATION WITH KERATINOCYTES ARE
PLACED IN A MATRIX OF COLLAGEN OR SYNTHETIC MATERIALS
• MATRIX ACTS LIKE A SCAFFOLD TO HELP CELLS ORGANIZE TO FORM A
FOLLICLE AND DIRECT ITS GROWTH

9. Challenges
• There happens to be a need to determine the most appropriate follicular
components to be.
• the extracted cells must be successfully cultured outside the body
• a cell matrix might be needed to keep them properly aligned while they
are growing
• the cells must be strongly injected into the scalp of the recipient so that
they will consistently induce the hair to grow

10. http://www.clinicspots.com/hair-
loss/India/Kolkata/hair-transplant