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Excursie KHK Geel 5TW: bananenbier 6TW:  DNA fingerprinting
Vertrek… ,[object Object]
5 TW  excursie bananen  22/01/08
Bereiding… ,[object Object]
Bereiding ,[object Object]
Dit is het resultaat van een dagje bier brouwen in het KHK in Geel.  We maakten een aantal flesjes bananenbier, met een zelfontworpen etiket. Dan rest er ons enkel nog het pintje leeg te drinken, na gisting. Schol!
[object Object]
Tentoonstelling:
De tentoonstelling bestond uit grote posters tegen de muur. Elke poster bevatte een ander interessant weetje over de bananen, hier ziet u onder gids die uitleg geeft over de bananen.
[object Object],[object Object]
schimmel
tentoonstelling ,[object Object]
tentoonstelling ,[object Object]
tentoonstelling ,[object Object],[object Object]
De banaan: een bron van gezondheid ,[object Object],[object Object],[object Object],[object Object]
Einde tentoonstelling ,[object Object]
Einde tentoonstelling ,[object Object]
[object Object]
Chromosomen ,[object Object]
In het microscopielokaal hebben we chromosomen bestudeerd. We hebben de chromosomen kunnen bekijken van mensen (46 chromosomen), grassen, diploïde bananen (22chromosomen) en ook triploïde bananen (33chromosomen). Bij de chromosomen van de grassen was duidelijk de X-vorm te zien. Chromosomen gezien door de microscoop
Voorbereiden PCR-reactie
De PCR-reactie (Polymerase Chain Reaction ofwel polymerase-kettingreactie)   ,[object Object]
PCR-TECHNIEK
PCR-TECHNIEK ,[object Object]
Het maken van de agarosegel-elektroforese ,[object Object],[object Object],[object Object]
We voegen een kleurstof,sucrose en EDTA toe aan het DNA-staal. De kleurstof was broomfenolblauw, de sucrose dient om ons reactie-mengsel te verzwaren en EDTA is een ionenvanger die de enzymewerking stopt.
overzicht
Het mengsel, met het DNA van de banaan, in de inkepingen van de gel onder de bufferoplossing injecteren ,[object Object]
Analyse van de PCR producten ,[object Object],[object Object]
Op deze foto zie je het uiteindelijke resultaat, de DNA fingerprint van de verschillende bananensoorten. Elke bananensoort heeft een specifieke lengte behaald tijdens de proef. Het ene blokje is al wat verder geraakt dan het andere. De lengte van de fragmenten situeert zich tussen 600 en 1800 basenparen.
[object Object]

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Fotoverslag Khk Geel

  • 1. Excursie KHK Geel 5TW: bananenbier 6TW: DNA fingerprinting
  • 2.
  • 3. 5 TW excursie bananen 22/01/08
  • 4.
  • 5.
  • 6. Dit is het resultaat van een dagje bier brouwen in het KHK in Geel. We maakten een aantal flesjes bananenbier, met een zelfontworpen etiket. Dan rest er ons enkel nog het pintje leeg te drinken, na gisting. Schol!
  • 7.
  • 9. De tentoonstelling bestond uit grote posters tegen de muur. Elke poster bevatte een ander interessant weetje over de bananen, hier ziet u onder gids die uitleg geeft over de bananen.
  • 10.
  • 12.
  • 13.
  • 14.
  • 15.
  • 16.
  • 17.
  • 18.
  • 19.
  • 20. In het microscopielokaal hebben we chromosomen bestudeerd. We hebben de chromosomen kunnen bekijken van mensen (46 chromosomen), grassen, diploïde bananen (22chromosomen) en ook triploïde bananen (33chromosomen). Bij de chromosomen van de grassen was duidelijk de X-vorm te zien. Chromosomen gezien door de microscoop
  • 22.
  • 24.
  • 25.
  • 26. We voegen een kleurstof,sucrose en EDTA toe aan het DNA-staal. De kleurstof was broomfenolblauw, de sucrose dient om ons reactie-mengsel te verzwaren en EDTA is een ionenvanger die de enzymewerking stopt.
  • 28.
  • 29.
  • 30. Op deze foto zie je het uiteindelijke resultaat, de DNA fingerprint van de verschillende bananensoorten. Elke bananensoort heeft een specifieke lengte behaald tijdens de proef. Het ene blokje is al wat verder geraakt dan het andere. De lengte van de fragmenten situeert zich tussen 600 en 1800 basenparen.
  • 31.