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Some Basics of DNA Sequencing
Let us consider a very simplified example: 
Let us say that our “gene” was the three-base 
sequence: ATG 
Since the beginning of any read is a bit noisy, 
we want to start sequencing before the beginning 
of our gene and beyond or after the end of our 
gene. So, we will include the two bases upstream of 
our gene and two bases downstream of the end of 
our gene: 
TTATGCA 
The rest we will do on the board.

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DNA Sequencing Lecture

  • 1. Some Basics of DNA Sequencing
  • 2.
  • 3. Let us consider a very simplified example: Let us say that our “gene” was the three-base sequence: ATG Since the beginning of any read is a bit noisy, we want to start sequencing before the beginning of our gene and beyond or after the end of our gene. So, we will include the two bases upstream of our gene and two bases downstream of the end of our gene: TTATGCA The rest we will do on the board.