1. PETUNJUK PENGGUNAAN
POLARIMETER POLAX 2L (ATAGO)
Specific Rotation Measurements
• Press and hold both the “TEMP” button and either the “R + ” rotate right
handed.
• button or the “L – ” rotate left handed button to set the front panel display to
“0.00”
• Open sample chamber cover and remove observation tube.
• Rinse observation tube three times with sample solution to be tested,
overfill tube with sample solution until it forms a meniscus. Note: pour
slowly to avoid formation of bubbles.
• Turn observation tube upside down and tap tube gently to observe any
bubbles. For large bubbles, observation tube should be re-filled with
sample solution to be tested. Place filled observation tube in polarimeter
and close sample chamber cover
• If the right semicircular field is brighter, then press and hold both the
“TEMP” button and the “R + ” rotate right handed button to approximately
equalize the brightness of the right and left translucent semicircular fields
while observing the fields through the eyepiece
• If the left semicircular field is brighter, then press and hold both the
“TEMP” button and the “L – ” rotate left handed button to approximately
equalize the brightness of the right and left translucent semicircular fields
while observing the fields through the eyepiece
• Press the the “R + ” rotate right handed button or the “L – ” rotate left handed
button to fine adjust the brightness of the right and left translucent
semicircular fields while observing the fields through the eyepiece
• Record angle of rotation displayed on front panel and calculate specific
rotation using following formula:
“a” is angle of rotation
“l” is length of observation tube in decimeters ( 2 dm)
“c” is grams of sample per 100 ml
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