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Three students, Habib, Vikki and Claire, in Biol 107 ran their samples of Hindlil cut plasmid
DNA side by side on the same gel and got the results shown below. Habib's bands are not as
bright as Claire's and Vikki has no DNA. Their lab instructor has asked them to consider where
in the mini-prep procedure Habib and Vikki may have gone wrong. From the list of mistakes
below, choose one mistake that could account for Habib's results and one mistake that could
account for Vikkis results. Explain why they would lead to less bright bands for Habib and no
DNA for Vikki. - Using 1.5ml of E.coll instead of 3ml - Not adding lysis buffer - Washing with
PE first and then PB - Not adding enough Hindili - Not adding enough SyberSate - Vortexing
their tubes when they werent supposed to - Transferring the white pellet to the filter cartridge

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Three students, Habib, Vikki and Claire, in Biol 107 ran their sample.pdf

  • 1. Three students, Habib, Vikki and Claire, in Biol 107 ran their samples of Hindlil cut plasmid DNA side by side on the same gel and got the results shown below. Habib's bands are not as bright as Claire's and Vikki has no DNA. Their lab instructor has asked them to consider where in the mini-prep procedure Habib and Vikki may have gone wrong. From the list of mistakes below, choose one mistake that could account for Habib's results and one mistake that could account for Vikkis results. Explain why they would lead to less bright bands for Habib and no DNA for Vikki. - Using 1.5ml of E.coll instead of 3ml - Not adding lysis buffer - Washing with PE first and then PB - Not adding enough Hindili - Not adding enough SyberSate - Vortexing their tubes when they werent supposed to - Transferring the white pellet to the filter cartridge