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1. Trieste, 16-17-18 giugno 2016
L’importanza di un colorante coesivo
in cromovitrectomia
Prof. Cesare Mariotti
Università Politecnica delle Marche
Clinica Oculistica

2. The term ‘‘chromovitrectomy’’ refers to the use of vital dyes during
vitreoretinal surgery to assist in the identification of pre-retinal
tissues and membranes.
Introduction
Trieste, 16-17-18 giugno 2016

3. Staining agents may be classified according to their
capacity to stain different structures:
- Epiretinal membranes
- Inner Limiting membranes
- Vitreous: The crystals of the steroid adhere to the acellular
tissue, thereby enabling a clear contrast between the
empty vitreous cavity compared to areas with the vitreous fibers
Introduction
Trieste, 16-17-18 giugno 2016
Staining agents may also be classified according to their physical and chemical
properties:
Cohesion is a key property for vital dye

4. “Cohesion”: Physics. the molecular force between particles
within a body or substance that acts to unite them.
Trieste, 16-17-18 giugno 2016
Introduction
Le forze intermolecolari contribuiscono alla coesione del prodotto

5. Le forze intermolecolari contribuiscono alla coesione del prodotto
Più forti sono le interazioni intermolecolari più coesivo è il prodotto

6. Dye 2 is more cohesive
compared to Dye 1
DYE 1 DYE 2
Trieste, 16-17-18 giugno 2016

7. Comparison of different behavior of trypan blue allo 0.18% e blulife allo 0,03% and
other dye available in the market
TWIN DYE 1 DYE 2
Trieste, 16-17-18 giugno 2016

8. Clinical use
Trieste, 16-17-18 giugno 2016

9. Clinical use
Trieste, 16-17-18 giugno 2016

10. Clinical use
Trieste, 16-17-18 giugno 2016

11. • Cohesive properties of trypan blue 0.18% and blulife 0,03% help the surgeon to:
o Reach the area to be stained without dispersion
o Direct the dye precisely on the area of coloration
o Remove the dye without excessive dispersion
Conclusion
Trieste, 16-17-18 giugno 2016