Palestra proferida no Satélite sobre potencial terapêutico de canabinóides da ICRS no Chipre 2008

1. FACILITATION OF CONTEXTUAL FEAR MEMORY EXTINCTION AND ANTI-ANXIOGENIC EFFECTS OF AM404 AND CANNABIDIOL IN CONDITIONED RATS Fabrício Pamplona, on behalf of Prof. Reinaldo N. Takahashi Depto. de Farmacologia, CCB, UFSC, Florianópolis – SC, Brazil Therapeutic Potential of the Cannabinoids: Present and Future

6. Conditioned fear responses have been extensively used to understand how aversive memories are formed and as putative animal models of disorders such as PTSD. Conditioned fear PTSD C O N T E X T U A L F E A R vs P T S D

7. C A N N A B I N O I D S AND FEAR M E M O R I E S

8. Pacher et al (2006) C A N N A B I N O I D S USED

9. Pacher et al (2006) C A N N A B I N O I D S USED

10. stereotaxic surgery Day 1 (conditioning) : 3 min  shock (1.5 mA, 1s)  1 min Day 2-4 (extinction) : 9 min context exposure. 3 X at 24 h intervals Freezing evaluation Day 5 (drug-free test) : 3 min context exposure Freezing evaluation C O N T E X T U A L F E A R C O N D I T I O N I N G

11. Experiment 1 : AM404 (0.2, 1, 2 ug/uL, i.c.v.), CBD (0.2, 1, 2 ug/uL, i.c.v.) or control (10% DMSO) 5min before extinction sessions Experiment 2 : SR141716A (0.2 mg/kg, i.p.) 20 min before AM404 or CBD i.c.v. injections Experiment 3 : Capsazepine (5 ug/uL, i.c.v.) 5 min before AM404 or CBD i.c.v. injections stereotaxic surgery Day 1 (conditioning) : 3 min  shock (1.5 mA, 1s)  1 min Day 2-4 (extinction) : 9 min context exposure. 3 X at 24 h intervals Day 5 (drug free test) : 3 min context exposure Freezing evaluation Freezing evaluation C O N T E X T U A L F E A R C O N D I T I O N I N G

12. Experiment 1: Effects of AM404 and CBD on contextual fear extinction R E S U L T S

13. Experiment 2: Role of CB 1 cannabinoid receptors R E S U L T S

14. Experiment 3: Role of TRPV 1 vanilloid receptors R E S U L T S

15. Possible contribution of anxiolytic-like effects? R E S U L T S

16. Experiment 4: Effects of DZP on contextual fear extinction R E S U L T S

17. Experiment 5: Effects of AM404, CBD and DZP in the fear-potentiated EPM test. R E S U L T S

18. Experiment 5: Effects of AM404, CBD and DZP in the fear-potentiated EPM test. R E S U L T S

19. Experiment 5: Effects of AM404, CBD and DZP in the fear-potentiated EPM test. R E S U L T S

20. Experiment 5: Effects of AM404, CBD and DZP in the fear-potentiated EPM test. R E S U L T S

22. Rafael Bitencourt PhD student Fabrício Pamplona PhD student Prof. Dr. Reinaldo N. Takahashi CANNABINOID BOYS A C K N O W L E D G E M E N T S Hi Rik, Thanks a lot for your help Muito Obrigado!