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Maximize CHO Cell Culture Performance with InVitria\'s Zap-CHO


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InVitria\'s Zap-CHO is a robust CHO cell culture media supplement designed to maximize CHO cell growth and productivity.

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Maximize CHO Cell Culture Performance with InVitria\'s Zap-CHO

  1. 1. Zap-CHO Media Supplement Maximizes the Growth and Productivity of CHO Cells<br />Steve Pettit, Ph.D<br />Director of Cell Culture Development<br />InVitria<br /><br />(800) 916-8311<br />
  2. 2. What is Zap-CHO?<br />Zap-CHO is:<br />A combination of defined recombinant proteins that maximize CHO growth and productivity<br />Well defined and consistent from lot-to-lot<br />Cost effective<br />A FBS replacement for performance - without the animal source<br />ZAP-CHO is NOT:<br />A hydrolysate<br />
  3. 3. Zap-CHO is Effective Across Media Types<br />Relative Increase in Productivity<br />820 810<br /> Protein Free Serum Free Chemically Defined Serum Free Protein Free Chemically Defined<br />
  4. 4. Improved Growth Kinetics of CHO with Zap-CHO <br />10% FBS<br />No Additive<br />
  5. 5. Average Increase in Growth and Productivity<br />*InVitria tests comparing the performance of Zap-CHO vs. hydrolysates and IGF1 show that Zap-CHO outperforms both of these supplements.<br />
  6. 6. Zap-CHO Proteins are Removed by Protein A<br />Reducing SDS-PAGE<br />75 KDa -<br />Heavy Chain<br />50 Kda -<br />Light Chain<br />25 Kda -<br />
  7. 7. Advantages & Applications of Zap-CHO<br />Advantages<br /><ul><li>Faster cell growth
  8. 8. Higher peak cell density
  9. 9. Reduced apoptosis
  10. 10. Increased viability
  11. 11. Maximized productivity
  12. 12. Removed by Protein A column during downstream purification</li></ul>Applications<br /><ul><li>Clone selection – Improve growth and shorten timelines
  13. 13. Seed train expansion – Shorten time from cell bank to bioreactor
  14. 14. Cell adaptation– Shorten timelines for media adaptation
  15. 15. Bioproduction– Maximize antibody titre</li></li></ul><li>To Learn More About Zap-CHO<br />To Request a Zap-CHO Scientific Poster or to Order a Sample of Zap-CHO <br /><ul><li>Online:
  16. 16. By Email:
  17. 17. By Phone: 1-800-916-8311</li>