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VITAE
Dolores J. Feld Takemoto
Citizenship: U.S.
Position: Professor Emeritus of Biochemistry
Dept. of Biochemistry
Kansas State University
http://www.k-state.edu/bmb/department/directory/takemoto/index.html
dtak@ksu.edu or dtakit@gmail.com (email)
(785) 410-1277 (Cell); Skype: dtakit
Home Address: 238 North Ave., Aptos, CA 95003
Degrees: B.S. - Ball State University, Muncie, IN - Biology - 5/71
MS. - Colorado State University, Ft. Collins, CO - Microbiology - 8/73
Ph.D. - University of Southern California, Los Angeles, CA – Molecular
Biology - 1/79
Positions Held:
1/79 - 8/80 Research Associate, Biochemistry Department, Kansas State University
8/81 - 8/84 Visiting Assistant Professor – Biochemistry, Kansas State University
8/84 - 8/88 Assistant Professor - Biochemistry Department, Kansas State University
8/88 - 8/94 Associate Professor - Biochemistry Department, Kansas State University
8/94 – 8/11 Professor - Biochemistry Department, Kansas State University
8/11 − pres. Professor Emeritus – Biochemistry, Kansas State University
8/11 – pres. Science Editor, Bioscience Writers (freelance)
10/11 –pres. Science Editor, Edanz Group (freelance)
5/13 – pres. Science Editor, Compuscript (freelance)
Expertise with editing in clinical ophthalmology and all areas of basic research in the
biological sciences.
Author: Gaining Weight? High Fructose Corn Syrup and Obesity (Jan 2012), Balboa Press

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Training:
2/75 - 1/79 - Graduate Student, Dept. Of Cell and Molecular Biology, University of
Southern California.
8/73 - 2/75 - Senior Research Technician, Dept. of Physiology and Biophysics,
Colorado State University.
9/71 - 8/73 - Graduate Student, Dept. of Microbiology, Colorado State Univ.
Previous Writing Experience - Academic:
► Served on University Compliance Committees for Human Use, Radiation Safety, and
Animal Approval – Compliance form writing
► Successfully funded from numerous agencies for 32 yrs as PI. NIH grants writing.
► Served in past and present on NSF and NIH Study Sections – reviewing/editing grants
► Managed a research lab of 10-12 individuals, including writing papers, grants,
managing the direction and budgets of multiple grants, most from those with English as
a second language, reviewer for numerous scientific journals.
► Served for 10 years on the NEI Small Business Study Section for SBIR grants.
► Author of over 100 peer reviewed publications.
► Co-author of a Biochemistry textbook and lab manual (Kendall Hunt Publishing)
Research Expertise
► Cell signaling and protection from aging, diabetes, and hypoxic damage in the eye.
Biochemistry
► Development of treatments for neural and eye diseases including, nanoparticle delivery,
stem cells, growth factors, and gap junction inhibitors. Protein structure/function, Cell

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Biology
Honors and Memberships:
American Cancer Society Post-doctoral Fellowship 1979-81
Leukemia Society of America Special Fellowship 1981-83
International Union Against Cancer - Technology Transfer Award 1984
New Investigator Research Award - NIH 1981-83
NIH Senior Fellowship (sabbatical leave) 1991
Invited Speaker - FASEB Conference on Vision Chemistry 1991
Member - Association for Research in Vision and Ophthalmology
Member - American Society for Biochemistry and Molecular Biology
Member - International Congress for Eye Research
Member - NIH Study Section - Biomedical Sciences, 1985-1989
Member - NSF Advisory Panel - Biochemistry Program, 1992 & 1993
Member – SBIR/STTR Study Section – NEI – 2000 - 2010
KSU Professorial Performance Award – 2007
Commerce Bank Distinguished Graduate Faculty Award – 2008
Fellow of the Association for Research in Vision and Ophthalmology – 2010
(Currently a member)
External Grant Support: (approximately $500,250/yr total )
1RO1 EY13421 (Dolores J. Takemoto, Principal Investigator)
Dates of Approved Project: 5/01/01 – 05/31/12
Source: National Eye Institute
Total Annual Current Costs: $250,000/ current yr direct cost
Title of Project: Protein Kinase C in the Lens
Past Support ( and writing expertise):
Grants (listed in chronological order, with approx funding levels)

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Regulation of Cyclic GMP in Human Lymphocytes, Leukemia Research Foundation; 7/79-
6/80;
Co-P.I. - D. Takemoto. approx. $15,000/yr
Control of Cyclic GMP in Human Leukemia, American Cancer Society - Kansas Division; #
IN-115; 1/80 - 12/80; Principal Investigator - D. Takemoto. $5000
Rod Outer Segment Disc Membrane: Relationship to Retinal Dystrophies, National Society to
Prevent Blindness; 81-83; Principal Investigator, D. Takemoto. $10,000/yr
The Control of Cyclic GMP in Human Lymphocytes; 80-83; NIH # CA 27500; Principal
Investigator - D. Takemoto - New Investigator Research Award. $100,000/yr, New
Investigator Research Award.
Cyclic Nucleotide Phosphodiesterase Regulation in Heart; 84-86; American Heart Association
- Kansas Division; Principal Investigator - D. Takemoto. $15,000/yr
ROS Disc Membrane Structure/Function Relationships; 85-86; Fight for Sight, Inc.; GA-
85032; Principal Investigator, D. Takemoto. $10,000
ROS Disc Membrane Changes During Retinal Degeneration ; 85-88; NIH-EY5623; Principal
Investigator, D. Takemoto. $85,000/yr
ROS Disc Membrane Changes During Retinal Degeneration ; 85-88; NIH-EY-5623-S1; was
awarded 100% salary support as an R.C.D.A. to D. Takemoto - Principal Investigator - NEI
does not award RCDAs. $200,000 total for salary and fringe.
Structure/Function Relationships of Rod Disc Membranes; 9/87-9/92; NIH EY6490; P.I.
Dolores Takemoto. $100,000/yr
Cloning and Site-directed Mutagenesis of the Inhibitory Phosphodiesterase Subunit; 7/89-7/90;
Kansas Heart Assoc.; P.I. Dolores Takemoto. $25,000/yr
Structure of Cyclic Nucleotide Phosphodiesterase, 90-92; Kansas Heart Assoc.; P.I. Dolores
Takemoto. $25,000/yr
Synthesis and Antitumor Activity of Terpenoids, 89-92; NIH GM 36336; (Co-Principal
Investigator - D. Takemoto), Principal Investigator - Dr. Duy Hua, Chemistry Dept,
$150,000/yr.
The Role of Na+
- K+
-ATPase in Diabetic Retinopathy, 92-93, Great Plains Diabetes
Research, D. Takemoto, P.I. $5,000.
Structure of Cyclic Nucleotide Phosphodiesterase, 92-95, American Heart Association -
National, D. Takemoto, P.I. $50,000/yr

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"Protein Kinase C and Signal Transduction", American Heart Association - 94 - 96, PI,
D. Takemoto. $25,000/yr
“Antisense Treatment of Diabetic Retinopathy”, 96-98, P.I., D.J. Takemoto; ISIS
Pharmaceuticals. An agreement with company, $25,000.
“Development of Novel Aldose Reductase Inhibitors and Antisense for Treatment of
Diabetic Retinopathy”, Group Incentive Award, KSU, 98-01, P.I. D. J. Takemoto, KSU.
$75,000/yr
“ Cancer Protective Effects of Wheats with Different Antioxidant Potentials”, 99-02, Kansas
Wheat Commission, P.I., D.J. Takemoto. $25,000/yr
“Expression of Therapeutic Proteins in Whole Lens”. 04-08, NIH/RO3/EY15670,
Dolores J. Takemoto, PI., $434,569.
Fellowships (To Takemoto)
Regulation of Cyclic GMP in Human Leukemic Lymphocytes, American Cancer Society
Postdoctoral Fellowship, 79-81. $12,500/yr
The Control of Cyclic GMP in Human Lymphocytes, Leukemia Society of America Special
Fellowship, 81-83. $15,000/yr
Structural Determination of the Phosphodiesterase Gamma Subunit by High Resolution NMR,
Senior Fellowship (for sabbatical) - NIH - 91 – with Professor C. Hilbers, University of
Nijmegan, The Netherlands. $21,500 as an award for my salary during my sabbatical.
Other
Member, Graduate Training Grant – Virology and Tumor Biology
91-00 – NIH – CA – 09418 –for support of graduate students P.I. – J.P. Perchellet
Sloan Foundation Mentoring Program for Women and Minorities in Science and Engineering,
95-00, Alfred Sloan Foundation - P.I.; Provosts Office (a program for faculty). I wrote the
original program, first ever, from the Sloan Foundation..
NSF 99-25 (Dolores J. Takemoto – Co-PI)
% Effort: 10% academic year
Dates of Approved Project: 9-01-9/04
Source: National Science Foundation
Total Direct Costs Annually: $183,148
Title: Women on the Prairie: Bringing Girls into Science through Environmental

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Stewardship
The mentoring program for women and minorities is still in effect at Kansas State Univ.
Fellowship Grant Support to Post/Pre Doctoral Fellows:
Synthesis of Antitumor Compounds; 7/88-7/96; Wesley Foundation Postdoctoral Program; for
salary of Dr. Kazuko Sakamoto, Dr. Nicolai Khramtsov, Dr. Igor Udovichenko and Dr.
Alexander Yakhnin. $20,000 / yr, each individual.
Domain Mapping of Retinal Phosphodiesterase; 12/01/89-11/30/90; F32EY06248-01 for
salary of Dr. Kristina Hennessy, $35,000/yr.
Member Training Grant - Virology and Tumor Biology Predoctoral, 86-96; CA-09418-
Richard Consigli, P.I. - past salaries of Karen Gonzalez, Jess Cunnick, and Lynn Wagner,
$10,000/yr each individual..
Structure of Recombinant and Mutant Phosphodiesterase; 7/93-6/95; American Heart
Association; for salary of Dr. Igor Udovichenko, $25,000/yr.
Protein Kinase C Gamma in the Lens; 8/01 – 8/04; National Eye Institute; Minority Supplement
for Dr. T. A. Nguyen, $46,000/yr.
Equipment Grants:
Small Instrumentation Program - Biomedical Research Support Funds; 7/89-6/90;
S15EY08449; P.I. Dolores Takemoto, for purchase of HPLC equipment, $30,000.
Large Equipment Grant – From NEI with L .Takemoto and G. Conrad – mini core grant from
NEI for $600,000 for purchase of Biacore, Typhoon and HPLC/MS in current proteomics lab.
Various equipment grants for teaching labs
3/07 – NIH supplement to EYRO1/13421, $30,000 for purchase of a single cell injector.
Publications:
1. Takemoto, D. J., Abel, J. H., Jr., Allen J. C. (1975) The metabolism of corticosterone in the
salt gland of the duck Anas platyrhnchos. Gen. Comp. Endo. 26:226-232.
2. Allen, J. C., Abel, J. H., Jr., and Takemoto, D. J. (1975) Effect of osmotic stress on serum
corticoid and plasma glucose levels in the duck Anas platyrhnchos. Gen. Comp. Endo.
26:209-216.

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3. Allen, J. C., Abel, J. H., Jr., and Takemoto, D. J. (1975) Uptake and binding of labeled
corticosterone by the salt gland of the duck Anas platyrhnchos. Gen. Comp. Endo. 26:217-
224.
4. Abel, J. H., Jr., Takemoto, D. J., Hoffman, D. H., McNeill, T., Kozlowski, G., Masken, J.,
and Sheridan, P. (1975) Corticoid uptake by the paraventricular nucleus in the hypothalamus of
the duck Anas platyrhnchos. Cell and Tissue Res. 161:285-291.
5. Takemoto, D. J. Lee, P., Kaplan S. A. and Appleman, M. M. (1978) Cyclic AMP
phosphodiesterase in human lymphocytes and lymphoblasts. J. Cyclic Nucl. Res. 4:123-132.
6. Takemoto, D. J., Kaplan, S., and Appleman, M. M. (1979) Cyclic guanosine 3', 5'-
monophosphate involvement in the alteration of phosphodiesterase activity in mitogen-stimulated
human lymphocytes. Biochem. Biophys. Res. Comm. 90:491-497.
7. Appleman, M. M., Ariano, M., Takemoto, D. J. and Whitson, R. (1982) Cyclic Nucleotide
Phosphodiesterase. In Handbook of Experimental Pharmacology, (Eds., Kababian, J. W. And
Nathanson, J. A.) Vol. 58, pp. 261-300. (Invited review).
8. Takemoto, D. J., Kresie, R., and Vaughn, D. (1980) Partial purification and characterization of
a guanylate cyclase inhibitor with cytotoxic properties from the bitter melon (Momordica
charantia). Biochem. Biophys. Res. Comm. 94:332-339.
9. Takemoto, D. J., Haley, B., Hansen, J., Pinkett, O., and Takemoto, L. J. (1981) GTPase from
ROD outer segments; characterization by photo affinity labeling and tryptic peptide mapping.
Biochem. Biophys. Res. Comm. 102:341-347.
10. Takemoto, D. J., Dunford, C., Vaughn, D., Kramer, K., Smith, A., and Powell, R. G., (1982)
Guanylate cyclase activity in human leukemic and normal lymphocytes: Enzyme inhibition and
cytotoxicity of plant extracts. Enzyme 27:179-188.
11. Takemoto, D. J. Dunford, C., and McMurray, M. (1982) The cytotoxic and cytostatic effects
of the bitter melon on human lymphocytes. Toxicon 20:593-599.
12. Takemoto, D. J., Hansen, J., Takemoto, L. J., and Houslay, M. (1982) Peptide mapping of
multiple forms of cyclic nucleotide phosphodiesterase. J. Biol. Chem. 257:14597-14599.
13. Takemoto, D. J. Jilka, C., and Kresie, R., (1982) Purification of a cytostatic factor from the
bitter melon (Momordica charantia). Preparative Biochemistry 12:355-375.
14. Takemoto, D. J., Jilka, C., Rockenbach, S., and Hughes, J. (1983) Purification and
characterization of a cytostatic factor with anti-viral activity from the bitter melon. Preparative
Biochemistry 13:371-393.

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15. Jilka, C., Strifler, B., Fortner, G.W., Hays, E., and Takemoto, D. J. (1983) In vivo anti-tumor
activity of the bitter melon. Cancer Research 43:5151-5155.
16. Takemoto, D. J., and Jilka C. (1983) Increased content of calmodulin in human leukemia cells.
Leukemia Research 7:97-100.
17. Takemoto, D. J., Hansen, J., Takemoto, L. J., Houslay, M., and Marchmont, R. J. (1984)
Peptide mapping of cyclic nucleotide phosphodiesterases, in Ad. Cycl. Nuc. And Protein Phos.
Res., Vol. 16:55-63
18. Takemoto, L. J., Hansen, J., Farber, D., Souza, D., and Takemoto, D. J. (1984) Cyclic GMP
phosphodiesterase from bovine retina. Biochem. J. 217:129-133.
19. McMurray, M., Hansen, J., Haley, B., Takemoto, D. J. and Takemoto, L. J. (1985) Cyclic
nucleotide phosphodiesterase. Biochem. J. 225:227-232.
20. Hansen, J., Takemoto, D. J., and Takemoto, L. (1985) Characterization of gamma crystallins
from the lens: production of monoclonal antibodies to human lens proteins. Lens Research.
2(3):207-219.
21. Takemoto, D. J., Takemoto, L. J., Hansen, J., and Morrison, D. (1985) Regulation of retinal
transducin by carboxyl-terminal peptides of rhodopsin. Biochem. J., 232:669-672.
22. Takemoto, D. J., Hansen, J., and Takemoto, L. J. (1985) Altered rhodopsin accessibility in the
retinal dystrophic mouse. BBRC 132:804-810.
23. Takemoto, D. J., Spooner, B., and Takemoto, L. J. (1985) Antisera to synthetic peptides of
bovine rhodopsin. Use as site-specific probes of disc membrane changes in retinal dystrophic
dogs. Biochem. Biophys. Res. Comm. 132:438-444.
24. Takemoto, D. J., Morrison, D., Davis, Lawrence C., and takemoto, L. J. (1986) C-Terminal
peptides of rhodopsin: determination of the optimum sequence for recognition of retinal
transducin. Biochem. J., 235:309-312.
25. Takemoto, D. J., Cunnick, J., and Takemoto, L. J. (1986) Altered phosphorylation of
rhodopsin in retinal dystrophic Irish Setters. Biochem. Biophys. Res. Comm., 135:1022-1028.
26. Takemoto, L. J., Kodama, T., and Takemoto, D. J., (1987) Covalent changes at the N-and C-
Terminal regions of gamma crystallin during aging of the normal human lens. Exp. Eye Res.,
45:207-214
27. Takemoto, L. J., Kodama, T. and Takemoto, D. J., (1987) Antisera to synthetic peptides of
MIP26K as probes of changes in opaque versus transparent regions within the same human
cataractous lens. Exp. Eye Res., 45:179-183.
28. Takemoto, L. J., Takemoto, D. J., Brown, G., Takehana, M., Smith, and Horwitz, J. (1987)

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Cleavage from the N-Terminal region of BBP crystallin during aging of the human lens. Exp.
Eye Res., 45:385-392.
29. Morrison, D., Rider, M. and Takemoto, D. J., (1987) Modulation of retinal transducin and
phosphodiesterase activities by synthetic peptides of the phosphodiesterase gamma subunit.
FEBS. Letters, 222:266-270.
30. Cunnick, J., Rider, M., Takemoto, L. J., and Takemoto, D. J. (1988). Rod/cone dysplasia
in Irish Setters: Presence of an altered rhodopsin. Biochemical Journal, 250:335-341.
31. Chang, C., Davis, L., Rider, M., and Takemoto, D. J. (1988) Characterization of nifH gene
mutations of Klebsiella pneumoniae, J. Bact. 170:4015-4022.
32. Montgomery, D., Cunnick, J., Coulter, M., Sinai-Zingde, G., Takemoto, D. J., and Hua, D.
(1988) Synthesis and In vitro cytotoxic activity of 1-phenyl-3-phenyl-amino-4 (p-
toluenesulfinyl)-trans-1,5-hexadiene. Leukemia Research, 12:591-596.
33. Komori, N., Rider, M., Takemoto, D. J., Shichi, H., and Matsumoto, H. (1988) ADP-
Ribosylation of bovine S-antigen by cholera toxin, BBRC, 156:1160-1165.
34. Morrison, D., Cunnick, J., Oppert, B. and Takemoto, D. J. (1989) Interaction of the -Subunit
of retinal rod outer segment phosphodiesterase with transducin. J. Biol. Chem. 264:11671-
11681.
35. Cunnick, J., and Takemoto, D. J. (1989) Analyses of proteins from rcdl Irish Setters, in
Inherited and Environmentally Induced Retinal Disorders Vol. 314:441-454 (Alan R. Liss,
Inc.).
36. Whalen, M., Bitensky, M., and Takemoto, D. J. (1990) The effect of the gamma subunit on the
kinetic parameters of the cGMP phosphodiesterase of bovine and frog retinal rod outer
segments, The Biochemical Journal, 265:655-658.
37. Cunnick, J., Hurt, D., Oppert, B., Sakamoto, K., and Takemoto, D. J. (1990) Binding of the
gamma-subunit of retinal rod outer segment phosphodiesterase with both transducin and the
catalytic subunits of phosphodiesterase. The Biochemical Journal, 271:721-727.
38. Takemoto, D. J. and Cunnick, J. (1990) Visual transduction in rod outer segments. A mini-
review. Cellular Signalling, 2:99-104.
39. Cunnick, J., Sakamoto, K., Chapes, S., Fortner, W., and Takemoto, D. J. (1990) Induction of
tumor cytotoxic immune cells using a protein from the Bitter Melon (Momordica charantia).
Cellular Immunology, 126:278-289.
40. Maxwell, S., Hurt, D., Brightman, A., and Takemoto, D. J. (1991) Identification and
purification of equine retinal S-antigen, Progress in Veterinary and Comparative Ophthalmology

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1:19-23.
41. Thirkill, C., Roth, A., Takemoto, D. J., Tyler, N., and Keltner, M. (1991) Antibody indications
of secondary and superimposed retinal hypersensitivity in retinitis pigmentosa, Amer. J. Ophth.
112:132-137.
42. Oppert, B., Cunnick, J., Hurt D., and Takemoto, D. J. (1991) Identification of the retinal cyclic
GMP phosphodiesterase inhibitory gamma subunit interaction site on the catalytic alpha-subunit,
J. Biol. Chem., 266:16607-16613.
43. Oppert, B. and Takemoto, D. J. (1991) Identification of the gamma-subunit interaction sites on
the retinal cyclic GMP phosphodiesterase beta-subunit, BBRC, 178:474-479.
44. Oppert, B., Gonzalez, K., Hurt, D., Cunnick, J., and Takemoto, D. J. (1991) Use of mutants to
define the function of the retinal cGMP phosphodiesterase inhibitory -subunit. BBRC,
181:306-309.
45. Maxwell, S., Hurt, D., Brightman, A., and Takemoto, D. J. (1991) Humoral responses to
retinal proteins in horses with recurrent uveitis, Progress in Veterinary and Comparative
Ophthalmology, 1:155-161.
46. Hua, D., Miao, S., Bravo, A., and Takemoto, D. J. (1991) Asymmetric Total Synthesis of (-)
Lupine and (+) Epilupine via -Sulfinyl Ketimine, Synthesis, 11:969-974.
47. Gonzalez, K., Cunnick, J., and Takemoto, D. J. (1991) Retinal Cyclic-GMP
Phosphodiesterase -subunit: Identification of Functional Residues in the Inhibitory Region.
BBRC, 181:1094-1096
48. Takemoto, D.J., Hurt, D., Oppert, B., and Cunnick, J. (1992) Domain Mapping of the retinal
cyclic GMP Phosphodiesterase gamma subunit: Function of the three exons of the gamma
subunit gene, Biochemical Journal, 281:637-643.
49. Cunnick, J. and Takemoto, D. J. (1992) Carriers of the Mouse rd Gene have Reduced Levels
of the Beta Subunit of the Retinal Cyclic GMP Phosphodiesterase, BBRC, 184:461-466.
50. Udovichenko, I., Cunnick, J., Gonzalez, K., and Takemoto, D. J. (1993) Phosphorylation of
Bovine Rod Photoreceptor cGMP Phosphodiesterase. Biochem. J., 295, 49-55.
51. Cunnick, J. and Takemoto, D. J. (1993) Bitter Melon (Momordica charantia) A review. J.
Naturopath Med., 4:16-21.
52. Takemoto, D. J., Gonzalez, K., Udovichenko, I., and Cunnick, J. (1993) Cyclic GMP-
regulated cyclic nucleotide phosphodiesterase. Cellular Signalling, 5:549-553.
53. Gonzalez, K., Udovichenko, I., Cunnick, J. and Takemoto, D. J. (1993) Protein Kinase C in

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Galactosemic and Tolrestat-Treated Lens Epithelial Cells, Current Eye Research, 12:373-377.
54. Cunnick, J., Twamley, C., Udovichenko, I., Gonzalez, K., and Takemoto, D. J. (1994)
Identification of a binding site on retinal transducin  for the phosphodiesterase inhibitory 
subunit. Biochem. J. 297:87-91.
55. Udovichenko, I. P., Cunnick, J., Gonzalez, K., and Takemoto, D. J. (1994) Functional effect of
phosphorylation of the photoreceptor phosphodiesterase inhibitory subunit by protein kinase C.
J. Biol. Chem. 269:9850-9856.
56. Udovichenko, I., Cunnick, J., Gonzalez, K., and Takemoto, D. J. (1994) The visual
transduction and the phosphoinositide system: a link, Invited Review, Cellular Signalling.
6:601-605.
57. Gonzalez, K., McVey, S., Cunnick, J., Udovichenko, I., and Takemoto, D. J. (1995) Acridine
orange differential staining of total DNA and RNA in normal and galactosemic lens epithelial
cells in culture using flow cytometry. Current Eye Res., 14:264-273.
58. Udovichenko, I., Cunnick, J., Gonzalez, K., Yakhnin, A. and Takemoto, D. J. (1996) Protein
Kinase C in Rod Outer Segments: effects of phosphorylation of the phosphodiesterase
inhibitory subunit. Biochem. J. 317:291-295.
59. Hua, D., Asaha, S., and Takemoto, D. J. (1997) Anticancer activities of 2,5,8,9-substituted 6-
oxo-1,2,3,4,5,6-hexahydrophenanthridines on multi-drug resistant phenotype cells. Anticancer
Research. 17:2435-2442.
60. Gonzalez, K., and Takemoto, D. J. (1998) Localization of protein kinase C in normal and
galactosemic bovine lens epithelial cells in culture. Histochem & Cell Biol. 110:89-94.
61. Takemoto, D. J. (1998) Effect of trichosanthin an anti-leukemia protein on normal mouse spleen
cells. Anticancer Research. 18:357-362.
62. Takemoto, D. J. (1998) Decreases in raf 1 levels in lens epithelial cells are partially
reversed by myo-inositol. Acta Diabetologica, 35:145-149.
63 Takemoto, D. J. (1999) Cyclic nucleotide phosphodiesterase: the other side of signal
transduction. Birkhauser Press: Ari Sittarramaya, ed., in Signal Transduction, invited book
chapter, Birkhauser Press.
64. Saleh, S. and Takemoto, D.J. (2000) Overexpression of protein kinase C gamma inhibits
gap junctional intercellular communication in the lens epithelial cells. Exp. Eye Res., 71:99-
102.
65. Wagner, L. and Takemoto, D.J. (2001) Protein kinase C alpha and gamma in N/N 1003a
rabbit lens epithelial cell differentiation. Molecular Vision, 7:57-62.

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66. Wagner, L. and Takemoto, D.J. (2001) PKC alpha and PKC gamma overexpression causes
lentoid body formation in the N/N 1003a rabbit lens epithelial cell line. Molecular Vision,
7:138-144.
67. Lewis, S., Karrer, J., Saleh, S., Chan, X., Tan, Z., Hua, D., McGill, J., Pang, Y.P., Fenwick,
B., Brightman, A., and Takemoto, D.J. (2001) Synthesis and evaluation of novel aldose
reductase inhibitors: effects on lens protein kinase C gamma. Molecular Vision, 7:164-171.
68. Saleh, S., Takemoto, L., Zoukhri, D., and Takemoto, D.J. (2001) PKC gamma
phosphorylation on connexin 46 in the lens cortex. Molecular Vision, 7:240-246.
69. Wagner, L., Saleh, S., Boyle, D., and Takemoto, D. (2002) Effect of Protein Kinase C
Gamma on Gap Junction Disassembly in Lens Epithelial Cells in Culture. Molecular Vision,
8:59-66.
70. Wagner, L., Fowler, V., and Takemoto, D. J. (2002) The Interaction and Phosphorylation of
Tropomodulin by Protein Kinase C Alpha in N/N 1003A Lens Epithelial Cells. Molecular
Vision, 8:394-406.
71. Drankhen, K., Carter, J., Madl, R., Klopfenstein, C., Padula, F., Lu, Y., Warren, T.,
Schmidt, N., and Takemoto, D. (2004) Anti-cancer Activity of Wheats with High
Polyphenolic Content. Nutrition and Cancer, 47:188-194.
72. Nguyen, A., Boyle, D., Wagner, L., Shinohara, T., and Takemoto, D. (2003) LEDGF
Activation of PKCγ and Gap Junction Disassembly in Lens Epithelial Cells. Exp. Eye Res.
76:565-572.
73. Lin, D., Boyle, D., and Takemoto, D. (2003) IGF-1-induced Phosphorylation of Connexin
43 by PKCγ: Regulation of Gap Junctions in Rabbit Lens Epithelial Cells. IOVS. 44:1160-
1168.
74. Takemoto, D., Harris, R., Brightman, A., McGill, J., Hua, D., Davidson, H., Fenwick, B.,
and Wagner, L. (2004) Normalization of PKCγ in Galactosemic Dogs by a Novel Aldose
Reductase Inhibitor. Veterinary Ophthalmology . 7:163-167.
75. Lin, D., Zhou, J., Zelenka, P., and Takemoto, D. (2003) Protein Kinase C Gamma
Regulation of Gap Junction Activity Through Caveolin-1 containing Lipid rafts. IOVS,
44:5259-5268.
76. Lin, D., Lobell, S., Jewell, A., and Takemoto, D. (2004) Differential Phosphorylation of
connexin 46 and connexin 50 by H2O2 activation of protein kinase C γ. Mol. Vis. 10:688-
695.
77. Nguyen, T.A., Takemoto, L., and Takemoto, D. (2004) Inhibition of gap junction activity

13. 13
through the release of the C1-domain of PKCγ from 14-3-3: Identification of PKCγ binding
sites. J. Biol. Chem. 279:52714-52725.
78. Takemoto, D., Davidson, H., and Takemoto, L. (2005) Expression of Superoxide
Dismutase in Whole Lens: Prevents Cataract Formation. Mol. Vis. 11:853-858.
79. Montelone, B., Dyer, R., and Takemoto, D. (2003) A Mentoring Program for females and
minority faculty members in science and engineering: Effectiveness and Status after 9 years.
J. Women and Minorities in Science and Engineering. 9:259-271.
80. Lin, D., and Takemoto, D. (2005) Oxidative activation of protein kinase Cγ through the C1
domain. J. Biol. Chem. 280:13682-13693.
81. Qu, H., Madl, R., Takemoto, D., Baybutt, R.,and Wang, W. (2005) Lignans are involved in
the antitumor activity of wheat bran in colon cancer SW480 cells. Nutrition and Cancer.
135:598-602.
82. Zampighi, G., Planells, A., Lin, D., and Takemoto, D. (2005) Regulation of Lens Cell-to-
cell Communication by activation of PKCγ and disassembly of Cx50 channels. IOVS 46:
3247-3255. (cover picture)
83. Lin, D., Barnett, M., Lobell, S., Madgwick, D., Shanks, D., Willard, L., Zampighi, G., and
Takemoto, D. (2006) PKCγ knockout mouse lenses are more susceptible to oxidative stress
damage. J. Exp. Biol. 209:4371- 4378.
84. Lin, D., Shanks, D., Prakash, O., and Takemoto, D. (2007) Protein kinase C γ mutations in
the C1B domain cause caspase-3-linked apoptosis in lens epithelial cells through gap
junctions. Exp Eye Res. 85:113-122 .
85. Akoyev, V., and Takemoto, D. (2007) ZO-1 is required for protein kinase C gamma-driven
disassembly of connexin43. Cellular Signalling. 19:958-967.
86. Yun, M., Barnett, M., S., Takemoto, D., Davidson, H. and Kompella, U. (2007) Human
serum albumin nanoparticles for gene delivery of Cu, Zn superoxide dismutase gene. Mol.
Vis. 13:746-757.
87. Lin, D., Harris, R., Zampighi, G., Davidson, H., Takemoto, D. (2007). Protein kinase Cγ
activation in the early streptozotocin diabetic rat lens. Current Eye Res. 32:523-532.
88. Barnett, M., and Takemoto, D. (2007) Protein kinase C as a stress sensor. Invited review.
Cellular Signalling. 19:1820-1829.
89. Lin, D., and Takemoto, D. (2007) Protection from ataxia-linked apoptosis by gap junction
inhibitors. Biochem. Biophys. Res. Comm. 362:982-987.

14. 14
90. Barnett, M., Lin, D., Akoyev, V., Willard, L., and Takemoto, D. (2008) Protein Kinase C
Epsilon activates lens mitochondrial cytochrome C oxidase subunit IV during hypoxia. Exp.
Eye Res., 86: 226-234.
91. Shi, A., Nguyen, T., Battina, S., Rana, S., Takemoto, D., Chiang, P., and Hua, D. (2008)
Synthesis and anti-breast cancer activities of substituted quinolines. Biorg. Med. Chem.
Lett. 18:3364-3368.
92. Das, S., Lin, D., Jena, A., Shi, A., Battina, S., Hua, D., Allbaugh, R., and Takemoto, D.
(2008) Protection of retinal cells from ischemia by a novel gap junction inhibitor. Biochem.
Biophy. Res. Comm. 373: 504-508.
93. Akoyev, V., Das, S., Jena, S., Grauer, L., and Takemoto, D. (2009) Hypoxia regulates the
activity of PKCε in the lens. Invest. Ophthalmol. Vis. Sci., 50:1271-1282..
94. Zhang, Y., Snider, A., Willard, L., Takemoto, D., and Lin, D. (2009) Loss of Purkinje cells
in the PKCγ transgenic mouse. Biochem. Biophys. Res. Commun. 378: 524-528.
95. Yevseyenkov, V., Das, S., Lin, D., Willard, L., Davidson, H., Sitaramayya, A., Giblin, F.,
Dang, L., and Takemoto, D. (2009) Loss of PKCγ in the knockout mouse causes increased
retinal sensitivity to hyperbaric oxygen, Arch. Ophthalmol. 127:500-506
96. Lauer, J., Banerjee, D., Shanks, D., Dai, H., Gong, Y., Prakash, O., and Takemoto, D.
(2010) NMR structure/function relationships of peptides corresponding to the C1B1 region
of PKCγ. Protein & Peptide Letters. 17:1-10.
97. Banerjee,D., Gakhar, G., Madgwick, D., Hurt, A., Takemoto, D., and Nguyen, T. (2010) A
novel role of gap junction connexin 46 protein to protect breast tumors from hypoxia.
International Journal of Cancer. 127:839-848.
98. Burr, D., Molina, S., Banerjee, D., Low, D., and Takemoto, D. (2011) Treatment with
connexin 46 siRNA suppresses the growth of human Y79 retinoblastoma cell xenografts in
vivo. Exp. Eye Res. 92:251-259.
99. Das, S., Wang, H., Molina, S., Martinez-Wittinghan, F., Jena, S., Bossman, L., Miller, K.,
Mathias, R., and Takemoto, D. (2011) PKCγ, role in lens differentiation and gap junction
coupling. Current Eye Research. 36:620-631.
100. Banerjee, D., Das, S., Molina, S., Madgwick, D., Jena, S., Bossman, L., Pal, D., and
Takemoto, D. (2011) Reciprocal relationship between connexin 46 and connexin 43. J. Biol.
Chem. 286:24519-24533.
101. Molina, S. and Takemoto, D (2012) The role of connexin 46 promoter in lens and other
hypoxic tissues. Communicative & Integrative Biology, 5:2, 1-4.

15. 15
Text Book
Biology and Society: Essays on the Impact of Chemical-Biology, with an
accompanying lab manual – Great River Technologies ePublishing Co. subsidiary of
Kendall Hunt, 2010. (Kendall-Hunt Publishing). J. Tomich and D. Takemoto.
Patents:
Methods of Treating Cataracts and Diabetic Retinopathy with Tricyclic Pyrones. Patent
No. US 6,916,824 B1, Date of patent, July 12, 2005, Duy Hua, Dolores Takemoto, Alan
Brightman, and Bradley Fenwick
Compounds Affecting Gap Junction Activity. Patent No. 8,809,368. Date 8/19/2014,
Dolores Takemoto, Duy Hua, and Thu Annelise Nguyen
Trade Book:
Gaining Weight? High Fructose Corn Syrup and Obesity; by Dee Takemoto and Joanne
McIntyre, Jan. 2012.(Balboa Press, Division of Hay House)