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HITUNG JUMLAH LEUKOSIT
1. Cari bilik hitung di mikroskop (obyek 10x)
                                    1/4 mm


                                   1 mm




                                             KOTAK BESAR


                                             KOTAK SEDANG
1/20 mm


          1/5 mm




      1 mm
2. Hisap darah dengan pipet leukosit sampai
   angka 1 (pengenceran 10x) atau sampai
   angka 0,5 (pengenceran 20x)
3. Hapuslah kelebihan darah yang melekat pada
   ujung pipet.

4. Masukkan ujung pipet dalam larutan Turk
   dan lar. Turk diisap perlahan-lahan sampai
   garis-tanda 11. Hati-hatilah jangan sampai
   terjadi gelembung udara.


      TURK
5. Kocoklah secara horizontal pipet yang diisi
   tadi selama 1-3 menit terus-menerus; jagalah
   jangan sampai ada cairan terbuang dari
   dalam pipet itu saat mengocok.

6. Buanglah 1-3 tetes dari pipet lekosit dan
   segeralah sentuhkan ujung pipet itu dengan
   sudut 30 derajat pada permukaan bilik hitung
   dengan menyinggung pinggir kaca penutup.
   Biarkan bilik hitung itu terisi cairan perlahan-
   lahan dengan daya kapilaritasnya sendiri.
7. Pakailah lensa objektif kecil, yaitu dengan
   pembesaran 10 x. Turunkan lensa kondensor
   atau kecilkan diafragma.
CARA MENGHITUNG LEUKOSIT

     N x P
∑=
      V

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HITUNG_LEUKOSIT

  • 2. 1. Cari bilik hitung di mikroskop (obyek 10x) 1/4 mm 1 mm KOTAK BESAR KOTAK SEDANG
  • 3. 1/20 mm 1/5 mm 1 mm
  • 4. 2. Hisap darah dengan pipet leukosit sampai angka 1 (pengenceran 10x) atau sampai angka 0,5 (pengenceran 20x)
  • 5. 3. Hapuslah kelebihan darah yang melekat pada ujung pipet. 4. Masukkan ujung pipet dalam larutan Turk dan lar. Turk diisap perlahan-lahan sampai garis-tanda 11. Hati-hatilah jangan sampai terjadi gelembung udara. TURK
  • 6. 5. Kocoklah secara horizontal pipet yang diisi tadi selama 1-3 menit terus-menerus; jagalah jangan sampai ada cairan terbuang dari dalam pipet itu saat mengocok. 6. Buanglah 1-3 tetes dari pipet lekosit dan segeralah sentuhkan ujung pipet itu dengan sudut 30 derajat pada permukaan bilik hitung dengan menyinggung pinggir kaca penutup. Biarkan bilik hitung itu terisi cairan perlahan- lahan dengan daya kapilaritasnya sendiri.
  • 7. 7. Pakailah lensa objektif kecil, yaitu dengan pembesaran 10 x. Turunkan lensa kondensor atau kecilkan diafragma.