Sophisticated Gene Synthesis & Cloning is Easy at Creative Biogene
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At Creative Biogene, we can synthesize and clone any DNA sequences, even complex, GC-rich, repeated, or long genes, into prokaryotic, eukaryotic or viral vectors.
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Sophisticated Gene Synthesis & Cloning is Easy at Creative Biogene
- 2. The Traditional Cloning • For a traditional cloning process, it is generally thought to be lengthy and complex for eight reasons, as follows: • Cloning strategy design • Primer Synthesis • PCR and restriction enzyme digestion • Gel extraction and ligation • Transformation and plasmid miniprep • Screen by enzymatic digestion • Sequencing reaction preparation • Sequence assembly analysis
- 3. Challenges • Either to solve those issues or skip to the next step, the process can still be challenge due to factors, such as a physical template, minor or major changes, intensive labor, unpredictable results, etc. All those procedures can drain your reagents and precious time that supposed to be used in more refined points.
- 4. Cloning at Creative Biogene • At Creative Biogene, we can synthesize and clone any DNA sequences, even complex, GC-rich, repeated, or long genes, into prokaryotic, eukaryotic or viral vectors. • Creative Biogene has accomplished hundreds of thousands of gene-synthesis & cloning projects for scientists over the world. • Creative Biogene’s gene synthesis and Cloning service is flexible to accommodate projects from single gene synthesis to whole genome synthesis.
- 5. •Codon Optimization for any given expression system. • Synthesis of genes of interest with or without tags, and cloning the target sequence into a standard vector. •Sub-cloning of your synthetic gene products into a vector of your choice. •Amplification of specific cDNA by PCR/RT- PCR and cloning into a standard vector or a vector of your choice.
- 6. Other services provided by Creative Biogene: •Custom Viral Service • RNAi Service • Gene Service • Aptamer Service • Plasmid DNA Production Service • Stable Cell Line Generation • Oligonucleotide Synthesis Service
- 7. Contact • USA 45-1 Ramsey Road, Shirley, NY 11967, USA Tel: 1-631-626-9181 Email：info@creative-biogene.com • United Kingdom St John's Innovation Centre, Cowley Road, Cambridge CB4 0WS, UK Tel: 44-207-048-3343 Email：info@creative-biogene.com • Web: http://www.creative-biogene.com/Services/Gene- Service/Gene-Synthesis-and-cloning-service.html