SlideShare a Scribd company logo
1 of 51
Download to read offline
9/24/2014 
1 
The Perio Tray is an FDA cleared, prescription 
medical device to place solutions of the dentist’s 
choice into the gingival sulcus or periodontal pocket. 
Flexible comfortable material for non-invasive 
delivery. 
The Perio Tray differs from other trays or mouth guards 
in that the flexible material is custom formed with 
specialized seals and extensions for the shape and 
depth of each pocket so that a gasket-like seal directs 
and maintains medication in the pocket long enough for 
medication to have therapeutic effect.
9/24/2014 
2 
Crevicular flow cleans out the pocket 
area 40 times per hour under healthy 
conditions and even more so when the 
pocket becomes infected 
With the biofilms attached to the tooth 
and tissue, these areas become even 
more resistant to being flushed out
9/24/2014 
3 
In Vitro PEROXIDE GEL EXPERIMENT 
Confocal micrograph, untreated control, 
3 days in vitro Streptococcus mutans 
(S. mutans, strain UA 159) biofilm. 
Confocal micrograph, 3 day in vitro S. 
mutans biofilm treated for 5 minutes with 
1.7% hydrogen peroxide gel. 
REPORT ON PEROXIDE GEL EXPERIMENT 
Confocal microscope, S. mutans biofilm 
treated for 10 minutes with 1.7% hydrogen 
peroxide. 
Confocal microscope, S. mutans treated 
with placebo gel without hydrogen peroxide. 
10 minutes BID as maintenance….TID during treatment 
So When? 
We recommend place them in prior to your am 
shower 
Then brush and do your regimen after 
Evening time is easy but it can be when you 
watch TV or really when you want 
Clean with your toothbrush and water
9/24/2014 
4
9/24/2014 
5
9/24/2014 
6
9/24/2014 
7 
Fluoride gels with high caries 
MI Paste with high caries patients or 
sensitivity 
Vibramycin for antibiotic usage in the trays 
when you want alternatives or patient wants 
alternatives to systemic 
Truly what you want in customized trays 
An explorer….a probe….traditional x-rays
9/24/2014 
8 
Tell me, and 
I will forget. 
Show me, and 
I will remember. 
Involve me, and 
I will understand.
9/24/2014 
9 
It has been suggested that the majority of all plans that 
go untreated are a direct result of the patient’s lack of 
understanding.
9/24/2014 
10 
15 Distal Occlusal Pit 31 Occlusal 18 Occlusal 
45 year old… 1 implant crown, 1 crown, 2 restorations on 3 and 14
9/24/2014 
11 
Visual diagnosis can be highly subjective, Kefley and Holt 
1993 
Dentin involvement can occur under an intact enamel layer 
Pitts, 1997 
On X-ray, detecting occlusal decay is only when there is 
dentin penetration by 2-3mm due to the thickness of the x-ray 
having to penetrate buccal/lingually the thickest 
portion of the tooth. 
Bite Wing x-rays can identify inter-proximal lesions 
earlier because x-rays pass through a narrower part of 
the tooth yet their sensitivity is still not excellent 
40-60% demineralization required to produce image 
Underestimates size or depth 
Insufficient to determine activity level 
Low sensitivity 
39% occlusal 
50% interproximal 
Bader et al 2001 
70% of overall adolescents experience tooth decay, and 
far more in lower income families 
90% of caries in pit and fissures, and most are in molars 
Pit and fissures account for 12.5% of the surface area of 
a tooth and yet this is where 88% of caries occur
9/24/2014 
12 
The overall annual attack rate for caries for all occlusal 
first permanent molar surfaces is 5.9% per year, while 
the approximal surfaces is 1.3% in a representative 
group of children, seven to fifteen years of age.
9/24/2014 
13 
Transference of infective S mutans to other sites? 
62% sensitivity / low reliability 
Loesche et al, J Dent Res 1979 
Hujoel et al, Caries Res 1995 
False positives & false negatives 
Lussi, Caries Res 1991 
Di Disrupts intact i surface f layer, l eliminating li i i potential i l f 
for 
reversal 
Al-Sehaibany showed tug back by an explorer 
was only 24% diagnostic, meaning that 76% of 
the time that tug back was present, there was no 
caries! 
Ekstrand showed that a sharp explorer tip can 
damage an early de-mineralized white spot 
lesion of the enamel by cavitating the surface 
. 
15 Distal Occlusal Pit 31 Occlusal 18 Occlusal 
15 and 31 slight sticks…18 no stick…Do you Drill? 
What is the patient’s caries risk? 
What does the patient want?
9/24/2014 
14 
64 
31 38 
15 Distal Occlusal Pit 31 Occlusal 18 Occlusal 
Yes……dark stained pits, DRILL or NOT 
to Drill? 
1.0 1.5 2.0 2.5 > 3.0 
Sound Enamel Deep Dentin Caries 
Beginning Enamel 
C i 
Deep Enamel Caries 
Dentin Caries 
Caries 
“Doppler Radar” for Caries Detection 
A Picture is Worth a Thousand Words 
Analysis of Spectra images Color Scale and Diagnostic Value
9/24/2014 
15 
D0 – sound fissure 
system 
D1‐D2 – Enamel 
Caries 
D3‐D4 – Dentin 
Caries 
Diss. Madani, 2004 Uni 
Jena 
Histological Clinical Analysis 
Nomenclature of Dental Lesions 
15 Distal Occlusal Pit 31 Occlusal 18 Occlusal 
Color Mapping with Air Techniques and 
Spectra 
15 Distal Occlusal Pit 31 Occlusal 18 Occlusal 
Infrared Technology…..Continuing to Advance
9/24/2014 
16 
15 Distal Occlusal Pit 31 Occlusal 18 Occlusal 
Minimal preparations…well into enamel patient preferred to 
treat early and avoid shots and future work 
Treatment with Equia or Low stress bulk fills or Activa 
•Non stress bearing Class I & II restorations 
•Deciduous teeth restorations 
•Geriatric restorations 
•Base / dentine replacement 
•Cervical (Class V) restorations 
•Core build up 
•Temporary fillings 
•ART (Atraumatic Restorative Technique)
9/24/2014 
17 
41 
37 
No stick 
Class 1’s 
Into Dentin 
Without major occlusal 
function
9/24/2014 
18
9/24/2014 
19 
Application of EQUIA Coat… 
...30-50 μm of penetration in to EQUIA Fil to fill up porosities 
…increases fracture toughness by 212% 
…increases flexural strength by 72% 
…increases flexural fatigue resistance 
…protects from acid erosion 
…improves aesthetics 
…takes long to wear off (6 months or more) 
EQUIA Coat 
EQUIA Fil 
No delamination layer at the 
interphase 
EQUIA Coat filling 
up porosities 
Depth of penetraition 30- 
50μm 
EQUIA Fil
9/24/2014 
20 
0,20 
0,15 
0,10 
0,05 
0,00 
Fuji IX GP EXTRA Fuji IX GP EXTRA + G-COAT 
PLUS 
Acid erosion / mm 
Extra Protection 
EQUIA Fil EQUIA Fil + 
EQUIA Coat 
EQUIA Coat protects from acid erosion, important in High Caries Risk 
119999 
patients… 
What happens 
when Coating 
wears off? 
What happens when EQUIA Coat Wears off? 
The EQUIA restoration undergoes a unique maturation effect attributed to 
saliva…
9/24/2014 
21 
1500 
1000 
500 
0 
0 20 40 60 80 100 
Fluoride release / μg cm-2 
Fuji Triage 
Fuji II LC improve 
Fuji IX GP FAST 
Fuji Filling LC 
EQUIA Fil 
202 
days 
Once coating wears off, fluoride recharge cycle is initiated... 
120 
100 
80 
60 
40 
20 
0 
1day 4 days 7 days 30 days 90 days 
μm 
Fuji IX Fuji IX GP Fast 
GIC have high early wear ……… however due to maturation a long term wear 
similar to composite 
I Placed in 1984
9/24/2014 
22 
207
9/24/2014 
23
9/24/2014 
24
9/24/2014 
25 
Chew the enclosed wax, then 
spit to the A line 
Add 1 drop of Reagent 1: 
Tap the container 15 times 
Add 4 drops of Reagent 2: 
Shake until saliva sample turns 
green 
Now Dispense 3 scales of saliva 
into the sample window and wait 
15 minutes 
The red arrow shows the pipet 
The yellow arrow shows the 
plastic vehicle we use to mix 
Drops from Solutions 1 and 2 to 
mix prior to testing 
The white plastic testing piece is 
how we evaluate Strep Mutans 
results 
1. Identification of Antigenic Epitopes in an Alanine-Rich Repeating Region of a 
Surface Protein Antigen of Streptococcus mutans. N. Okahashi, I. Takahashi, M. 
Nakai, H. Senpuku, T. Nisizawa and T. Koga. Infection and Immunity, Apr. 1993, 
vol. 61, N°4:p.1301-1306 
2. Rapid and quantitative detection of Streptococcus mutans with species specific 
monoclonal antibodies. W. Shi, A. Jewett, W.R. Hume. Hybridoma 1998;17:365- 
371. 
3. Simple and Rapid Immunoassay for the Estimation of Streptococcus mutans in 
Human Saliva. Y Matsumoto, N. Sugihara, M. Koseki, Y. Maki. Abstract –50th 
ORCA Congress, July 2-6, 2003, Konstanz, Germany 
4. Evaluation of Rapid Screening Test for S. mutans Using Species-specific 
Monoclonal Antibodies. Y. Maki, N. Sugihara and M. Koseki. Abstract 2077–81st 
General Session of IADR, June 25-28, 2003, Goteborg, Sweden
9/24/2014 
26 
After placing Reagent 1 and taping 
the container 15 times 
4 Drops of Reagent 2 are then 
added to the solution and again 
mixed until the saliva turns green 
The solution is THEN placed into the 
orifice via the pipet and you wait 15 
minutes 
Positive result - Either a faint or 
clear red line appearing means 
there are over 500,000 cfu/ml of 
S. MUTANS. 
How do you customize their therapy? 
Cariogenic biofilms thrive in acidic environments. 
These environments are partially created by 
eating and drinking certain foods. 
In order to fight mouth bacteria we need to work 
on the saliva as well as the surface of the teeth. 
By working on both aspects we can win the war of 
caries management.
9/24/2014 
27 
Real time (15 second ) 
inexpensive screening test 
for separating at risk from 
low risk individuals 
Cariscreen- Meter that when a swab with pellicle is 
placed inside , it can read the degree of 
cariogenicity of the patients biofilm. 
Depending on the reading, patient can be treated 
with their proprietary series of rinses. 
PH of the rinses are in the 8,9, and 10 range. Very 
basic to change the environment in the oral cavity. 
(“Measurement of ATP Bioluminescence from Oral Bacteria Contained in Dental Plaque: 
Basic Sciences and Clinical Assessments for Testing of Caries Risk” by Drs. R. Sauerwein, 
J. Kimmell, T. Finlayson, S. Fazilat, P. Pellegrini, I. Kasimi, D. Covell, P. Gagneja, J. Engle, 
K. Kutsch, T. Maier, and C.A. Machida , representing [not in corresponding order], 
Department of Integrative Biosciences, Academic DMD Program and OCTRI Research 
Fellowship Program, Department of Pediatric Dentistry, and Department of Orthodontics, 
School of Dentistry, Oregon Health & Science University, Portland, and Oral Biotech)
9/24/2014 
28 
What will affect the 
population of a biofilm 
Intense heat = 175 degrees F 
Complete mechanical debrasion 
Chemotherapeutics 
What will not affect the 
population of a biofilm 
Systemic antibiotics 
Brushing 
Flossing 
Introduction and Science 
What is Dental Caries: The New Paradigm 
pH Neutralization: Reduces growth and acid 
production of cariogenic bacteria, supports 
healthy oral bacteria growth 
Antibacterial: Significantly reduces total 
bacteria levels when high levels are identified 
Fluoride: Aids in remineralization and inhibits 
acid production of cariogenic bacteria 
Xylitol: Reduces growth and acid production of 
cariogenic bacteria 
Nano HA (Ca PO4): Aids in remineralization 
with nano particles of hydroxyapatite 
Targeted Therapy 
What is CTx? 
How the CTx Guide Applies 
to Products 
Number of agents equals CTx Score
9/24/2014 
29 
CTx4 Treatment Rinse 
pH Neutralization 
Antibacterial (.2% sodium 
hypochlorite) 
Fluoride (0.05%) 
Xylitol (Greater than 10%) 
3 Main Products 
CTx4 Gel 5000 
pH Neutralization 
Fluoride (1.1% Neutral 
Sodium Fluoride) 
HA Nano (Ca PO4) 
Xylitol (Greater than 
10%) 
CTx3 Rinse 
pH Neutralization 
Fluoride (0.05%) 
Xylitol (Greater than 10%)
9/24/2014 
30 
† Distributed exclusively by dental 
professionals 
† Extra-strength blend of ProBiora3® 
crowds out harmful bacteria around 
teeth and gums 
† Use once daily for 90 days after a 
professional cleaning 
† Extends the benefits of a dental prophy 
ProBiora3 – Ingredient in branded 
products
9/24/2014 
31 
† For companion pets 
† Freshens breath, cleans and 
whitens teeth 
†Tasteless, odorless powder 
† Strains weighted for gum 
health 
†ProBiora3 is the most comprehensive oral care probiotic technology 
available 
†Developed from research into dental caries and periodontal disease 
†Blend of 3 naturally occurring Streptococcal strains – S. oralis, S. uberis, 
S. rattus 
†ProBiora3 promotes: 
†dental and periodontal health 
†whiter teeth 
†fresher breath 
Probiora3: Product Overview 
ProBiora3 Marketed as Food Ingredient 
†Self-affirmed GRAS status 
†ProBiora3 is safe and effective 
15 peer-reviewed publicationsProBiora3
9/24/2014 
32 
Competitive Advantage 
†Strong scientific basis for safety and efficacy 
†ProBiora3 is the first and only 
comprehensive oral care probiotic on the 
market 
ProBiora3 – Competitive Advantage 
The ProBiora3 contains a formulation of beneficial 
bacteria, found in naturally healthy human mouths. 
S. oralis KJ3 
S. uberis KJ2 
S. rattus JH145 
S.rattus JH145 is a unique strain of streptococcus that does not produce lactic acid, and 
has been shown to successfully compete for nutrients and space on tooth surfaces with 
the native strain of streptococcus that produces lactic acid. 
ProBiora3 – How it works 
A spontaneous lactacte dehydrogenase deficient mutant of Streptococcus rattus for use 
as a probiotic in the prevention of dental caries. Hillman JD, McDonell E, Cramm T, 
Hillman CH, Zahradnik RT. Journal of Applied Microbiology 2009 Nov;107(5):1551-8. 
Epub 2009 Apr 24) 
ProBiora3 – How it works
9/24/2014 
33 
ProBiora3 – How it works
9/24/2014 
34 
Remin Pro supports remineralization. 
Remin Pro strengthens teeth. 
Remin Pro smooths tooth surfaces. 
Remin Pro neutralizes acids. 
Remin Pro desensitizes. 
Remin Pro tastes good. 
Contains all ingredients of 
natural tooth substance... 
Calcium ions 
Ph h i 
hydroxyapatite 
- Phosphate ions 
- Water/fluorides 
y yp 
[Ca5(PO4)3OH] 
• Remin Pro is ideal for 
protective tooth care 
Three Key Ingredients: 
- Fluoride (1450 ppm) - Hydroxyapatite (Calcium 
and Phosphate) = natural tooth substance 
- Xylitol (non-sugar sweetener with cariostatic effect) 
Natural tooth substance consists largely of hydroxyapatite (calcium and 
phosphate). 
It fills superficial enamel lesions and the tiniest irregularities that arise from 
erosion. 
Remin Pro adheres to the tooth substance and protects the tooth against 
demineralization and erosion. 
Smooths the surface by sealing dentin tubules. 
A smooth surface impairs 
the adhesion of bacterial plaque.
9/24/2014 
35 
Reduction of caries formation 
Sealing of tooth surfaces, desensitization 
Repair of incipient caries 
(remineralization) 
Delay of plaque formation and smoother 
surfaces 
Teeth whitening and gloss increase 
Kani T, Kani M, Isozaki A, Kato H, Fukuoka Y, Ohashi T, Tokumoto T: The 
effect of apatitecontaining dentifrices on artificial caries lesions. I Dent Health 
38, 364–366 (1988). 
Okashi T, Kani T, Isozaki A, Nishida A, Shintani H, Tokumoto T, Ishizu E, 
Kuwahara Y, Kani, M: Remineralization of artificial caries lesions by 
Hydroxyapatite. I Dent Health 41, 214 – 223 (1991). 
Nishio M, Kawamata H, Fujita K, Ishizaki T, Hayman R, Ikemi T: A new enamel 
restoring agent for use after PMTC. Posterpresentation 82nd General Session 
& Exhibition of the IADR / March 2004. 
Kawamata H, Nishio M, Fujita K, Ishizaki T, Hayman R, Ikemi T: 
Posterpresentation 82nd General Session & Exhibition of the IADR / March 
2004. 
Yamagishi K, Onuma K, Suzuki T, Okada F, Tagami J, Otsuki M, Senawangse 
P: Materials chemistry: A synthetic enamel for rapid tooth repair. Nature. 2005 
Feb 24; 433 (7028): 819. 
Sugar substitute comes from the Xylitol portion of 
a plant. 
Cannot be metabolized by acid producing 
cariogenic bacteria. 
Excreted as whole molecule. Natural population 
shift to helpful bacteria. 
6- 10 grams per day. 
Potentially toxic to dogs. 
Xlear, Dr. John’s candies, Xylitol USA
9/24/2014 
36 
The Glucose is absorbed by the Strep Mutan the 
Glucose then goes through a metabolic 
breakdown and ultimately gets excreted as 
acids and thus the pathway to caries 
Xylitol gets absorbed by the Strep Mutans, 
occupies or keeps the Strep Mutans busy but 
DOES NOT get broken down, and is excreted in 
whole… 
After short and long-term use, xylitol significantly reduces 
the amount of Strep Mutans in plaque and saliva. 
The growth of lactobacilli is reduced during long-term use of 
xylitol. 
Xylitol shows effects which promote tooth remineralization. 
Xylitol actively prevents acid production in dental 
plaque (enamel dissolves when the pH decreases below 
5.7). 
Regular xylitol consumption reduces the adhesive property 
of plaque, making it easier to brush off. 
http://www.ncbi.nlm.nih.gov/pubmed/12693818 An overview of studies about xylitol and dental caries 
Mäkinen K.K. The rocky road of xylitol to its clinical application. J. Dent. Res. 2000; 79: 1352. 
Lynch H, Milgrom P. Department of Dental Public Health Sciences, Northwest/Alaska Center to Reduce Oral Health Disparities, University of 
Washington, Seattle 98195-7475, USA. Xylitol and dental caries: an overview for clinicians. J Calif Dent Assoc. 2003 Mar;31(3):205-9. 
K.K. Mäkinen, K.P.Isotupa, T. Kivilompolo, P.L. Mäkinen, J.Toivanen, E. Söderling. Comparison of Erythritol and Xylitol Saliva Stimulants in the Control 
of Dental Plaque and Mutans Streptococci. Caries Research 2001;35:129-135. 
Kandelman D., Gagnon G. A 24-month clinical study of the incidence and progression of dental caries in relation to consumption of chewing gum 
containing xylitol in school preventive programs. J. Dent. Res. 1990; 69: 1771. 
Kauko K. Mäkinen, MS, Phd, Kauko P. Isotupa, DDS, Taina Kivilompolo, RDH, Pirkko-Liisa Mäkinen, MS, PhD, Satu Murtomaa, DDS, Juhani Petäjä, 
DDS, 
Jukka Toivanen, DDS, Eva Söderling, MS, PhD. The effect of polyol-combinant saliva stimulants on S. mutans levels in the plaque and saliva of 
patients with mental retardation. Special Care Dentistry 2002; 22(5): 187-193. 
Isotupa K.P., Gunn S., Chen C.Y., Lopatin D., Mäkinen K.K. Effect of polyol gums on dental plaque in orthodontic patients Am. J. Orthod. Dentofac. 
Orthop. 1995; 107: 497. 
Mäkinen K.K., Bennett C.A., Hujoel P.P., Isokangas P., Isotupa K.P., Pape H.R. Jr., Mäkinen P.-L. Xylitol gums and caries rates: A 40-month cohort study. 
J. Dent. Res. 1995; 74: 1904. 
Autio J.T., Courts F.J. Acceptance of the xylitol chewing gum regiment by preschool children and teachers in a Head Start program: a pilot study. 
Pediatr. Dent. 2001; 23:71. 
Autio J.T. Effect of xylitol chewing gum on salivary streptococcus mutans in preschool children. ASDC J. Dent. Child. 2002; 69: 81-6, 13. 
Alanen, P., Holsti, M.-L., Pienihäkkinen, K. (2000) Sealants and xylitol chewing gum are equal in caries prevention. Acta Odontol. Scand. 58:279- 
284. 
Caufield, P.W., Cutter, G.R., Dasanayake, A.P. (1993) Initial acquisition of mutans streptococci by infants: evidence for a discrete window of 
infectivity. J. Dent. Res. 72:37-45. 
Trahan, L. (1995) Xylitol: a review of its action on mutans streptococci and dental plaque – its significance. Int. Dent. J. 45:77-92. 
Scheinin, A., Mäkinen, K.K. (1971) The effect of various sugars on the formation and chemical composition of dental plaque. Int. Dent. J. 21:302- 
321. 
Scheinin, A., Mäkinen, K.K. (1972) Effect of sugars and sugar mixtures on dental plaque. Acta Odontol. Scand. 30:235-257. 
Scheinin, A., Mäkinen, K.K. (1975) Turku Sugar Studies I-XXI. Acta Odontol. Scand. 33 (Suppl. 70):1-349. 
Isokangas, P. (1987) Xylitol chewing gum in caries prevention. Academic Dissertation, University of Turku. 
Isokangas, P., Mäkinen, K.K., Tiekso, J., Alanen, P. (1993) Long-term effect of xylitol chewing gum in the prevention of dental caries: a follow-up 5 
years after termination of a prevention program. Caries Res. 27:495-498. 
Mäkinen, K.K., Bennett, C.A., Hujoel, P.P., Isokangas, P.J., Isotupa, K.P., Pape, H.R., Jr., Mäkinen, P.-L. (1995) Xylitol chewing gums and caries rates: a 
40-month cohort study. J. Dent. Res. 74:1904-1913. 
Hujoel, P.P., Mäkinen, K.K., Bennett, C.A., Isotupa, K.P., Isokangas, P.J., Allen, P., Mäkinen, P.-L. (1999) The optimum time to initiate habitual xylitol 
gum-chewing for obtaining long-term caries prevention. J. Dent. Res. 78:797-803.
9/24/2014 
37 
• With the finger 
• Using a soft toothbrush 
• Plaed in a tray 
Recaldent- Casein phosphopeptide (CPP)- amorphous calcium 
phosphate (ACP) 
Unique chemical in that it is substantive with a 3 hour half life 
MI Paste +----5 Calcium,3 phosphate,1 Fluoride—Ideal 
biochemical ratio to drive remineralization. 
Made from a milk protein, so contraindicated in patients with 
true milk allergy. 
OK for patients who are lactose intolerant 
Caution with young children. 
• Two Components 
1. ACP:Amorphous Calcium Phosphate 
2. CPP: Casein Phosphopeptide – natural 
occurring molecule 
• Milk Protein 
• Protects the ACP component 
• Delivery vehicle 
• Very sticky 
Functions: 
CPP-ACP attached to strep mutans 
making them non-viable 
252 
– Remineralization 
– Desensitization 
What is RecaldentTM (CPP-ACP)?
9/24/2014 
38 
Molecular model of the CPP-ACP 
complex. Casein phosphopeptide (CPP) 
is a milk derived protein able to bind 
calcium and phosphate ions and 
stabilize them as Amorphous Calcium 
Phosphate (ACP). 
ACP alone does not remain stable 
enough for effective 
long-term remineralization* 
253 
g 
*Cross KJ, Huq NL, Reynolds EC. Casein phosphopeptides in oral health—chemistry and clinical 
applications. Curr Pharm Des. 2007;13:793-800. 
CPP-ACP is added to the oral cavity. 
The ‘sticky’ CPP part binds readily to enamel, pellicle, 
plaque and soft tissue 
254 
H+ 
H+ 
H+ 
H+ 
Under acidic conditions, RecaldentTM is able to release 
Calcium and Phosphate ions 
255
9/24/2014 
39 
The free Ca and P ions move out of the CPP, enter the 
256 
enamel rods and reform into apatite crystals 
• Helps to maintain a state of super-saturation of Calcium and Phosphate 
ions on the tooth surface 
• RecaldentTM Depressing enamel demineralization 
is the ‘fluid enamel’ 
• Buffers plaque pH 
• which The longer CPP-restores ACP is maintained mineral in the mouth, the balance 
more effective the 
result 
257 
Improved Plaque Uptake and Enamel Remineralization by Fluoride with CPP-ACPE C Reynolds, N.J. COCHRANE, P. SHEN, 
F. CAI, G.D. WALKER, M.V. MORGAN, and C. REYNOLDS, Cooperative Research Centre for Oral Health Science, School of 
Dental Science, The University of Melbourne, Victoria, Australia Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) 
has been shown to slow the progression of caries and to remineralize enamel subsurface lesions. Objectives: The aim of 
the studies was to determine the ability of CPP-ACP to increase the incorporation of fluoride into supragingival plaque and 
to promote enamel remineralization in situ with acid resistant mineral. Methods: Randomized, double-blind cross-over studies 
were designed involving three mouthrinses and five toothpastes as follows: Mouthrinses (i) 2% CPP-ACP, (ii) 2% CPP-ACP 
plus 450 ppm F and (iii) 450 ppm F; and Toothpastes: (i) placebo, (ii) 1100 ppm F, (iii) 2800 ppm F, (iv) 2% CPP-ACP and 
(v) 2% CPP-ACP plus 1100 ppm F. The mouthrinses (15 ml) were used for 60 s, three times per day for 5 d and 
supragingival plaque collected and analyzed for F content. The toothpastes (1 g) were added to 4 ml water to form a slurry 
and used for 60 s four times per day for 14 days in an in situ remineralization model. Results: The addition of 2% CPP-ACP 
to the 450 ppm F rinse significantly increased the incorporation of fluoride ions into plaque where the plaque fluoride level 
(33.0±17.6 nmol/mg dry wt) was over double that obtained with the fluoride-only rinse (14.4±6.7 nmol/mg dry wt). 
Fluoride in the toothpaste slurry produced a dose-response related remineralization of subsurface enamel lesions. The 
toothpaste containing 2% CPP-ACP produced a level of remineralization (13.5%±1.5%) similar to the 2800 ppm F paste 
(15.5%±2.4%) and the paste containing 2% CPP-ACP plus 1100 ppm F was superior (21.0%±5.9%) to all other 
formulations in enamel lesion remineralization. Acid challenge of the remineralized lesions showed that the CPP-ACP/F 
mineralized lesions were relatively acid resistant. 
Conclusion: CPP-ACP promotes the incorporation of fluoride 
into plaque and sub-surface enamel producing effects 
superior to fluoride alone.
9/24/2014 
40 
Reduces sensitivity 
Decreases or eliminates white spots 
Assists in Caries management 
Children with cavitations and positive caries tests 
Children undergoing orthodontics. 
Adults with positive caries tests and cavitations 
People with sensitive teeth 
People taking medications that alter quantity and quality 
of saliva 
People undergoing a lot of restorative Dentistry.
9/24/2014 
41 
Documentation and progession control. 
262 
263 
Before treatment (cleaning) After treatment (cleaning)
9/24/2014 
42 
HCL Acid etch of all the teeth. 
Prema- Premier 
Opalustre- Ultradent 
Brown & white enamel 
demineralization 
PREMA® is applied using 10:1 
reduction angle – slow speed 
After a 14-day take home 
whitening treatment 
Immediately after 
PREMA® treatment 
Approximately 15 seconds per tooth- open the enamel pores. 
Following this a 37% phosphoric acid is applied to the teeth for 30 
seconds. 
After rinsing it off, Remin Pro( MI Paste), is applied in the office for 
5 minutes. 
This can be achieved with a gloved finger and leaving the cream 
in place or with a gloved finger and then placing the tray over the 
cream. 
Patient is sent home and told to wear the trays as much as possible 
daily with the cream in place.
9/24/2014 
43 
37% Phosphoric acid for 30 seconds followed by 5 
minutes of therapeutic cream. 
This is done weekly till 50-60% remineralization is 
seen. 
Now Pola Night (10% for kids or Pola Day CP 
35% for adults) is added to the protocol daily for 
ten days. 
Continue etch/cream protocol till desired result is 
achieved
9/24/2014 
44
9/24/2014 
45
9/24/2014 
46 
Futurabond® U 
The Dual-Cure 
Universal Adhesive! 
.
9/24/2014 
47 
self-etch 
selective-etch total-etch
9/24/2014 
48
9/24/2014 
49 
MI Paste in a bleaching tray for as long as 
she can wear it everyday. (Homework) 
Pola night — 15% started at week 8 for 2 
weeks. 
Continued with MI Paste
9/24/2014 
50
9/24/2014 
51

More Related Content

What's hot

ATRAUMATIC RESTORATIVE TREATMENT (ART)
ATRAUMATIC RESTORATIVE TREATMENT (ART) ATRAUMATIC RESTORATIVE TREATMENT (ART)
ATRAUMATIC RESTORATIVE TREATMENT (ART) DR YASMIN MOIDIN
 
Simplified and modified atraumatic restorative treatment
Simplified and modified atraumatic restorative treatmentSimplified and modified atraumatic restorative treatment
Simplified and modified atraumatic restorative treatmentHamed Gholami
 
7.recent advances in dentistry
7.recent advances in dentistry7.recent advances in dentistry
7.recent advances in dentistryChaitanya Pendyala
 
Conservative and pain free techniques in pediatric dentistry
Conservative and pain free techniques in pediatric dentistryConservative and pain free techniques in pediatric dentistry
Conservative and pain free techniques in pediatric dentistryDrNadhem
 
Pit and fissure sealants
Pit and fissure sealantsPit and fissure sealants
Pit and fissure sealantsAswini sekar
 
Diagnosis of dental caries / dental implant courses
Diagnosis of dental caries  / dental implant coursesDiagnosis of dental caries  / dental implant courses
Diagnosis of dental caries / dental implant coursesIndian dental academy
 
Root Canal Treatment for Patients & Dentist
Root Canal Treatment for Patients & DentistRoot Canal Treatment for Patients & Dentist
Root Canal Treatment for Patients & DentistAll Good Things
 
Cariesdiagnosis 111107222350-phpapp01
Cariesdiagnosis 111107222350-phpapp01Cariesdiagnosis 111107222350-phpapp01
Cariesdiagnosis 111107222350-phpapp01Joy Dutta
 
New Technologies in Caries Diagnosis: The Canary System in Pediatric Practice
New Technologies in Caries Diagnosis: The Canary System in Pediatric PracticeNew Technologies in Caries Diagnosis: The Canary System in Pediatric Practice
New Technologies in Caries Diagnosis: The Canary System in Pediatric PracticeDr Marielle Pariseau
 
Vital pulp therapy technique
Vital pulp therapy techniqueVital pulp therapy technique
Vital pulp therapy techniqueAli Khalaf
 
Deep caries / dental implant courses
Deep caries / dental implant coursesDeep caries / dental implant courses
Deep caries / dental implant coursesIndian dental academy
 
Isolation in endodontic
Isolation in endodonticIsolation in endodontic
Isolation in endodonticalaasm
 
ENDODONTIC EMERGENCIES
ENDODONTIC EMERGENCIESENDODONTIC EMERGENCIES
ENDODONTIC EMERGENCIESSelva Arockiam
 
Management of non surgical root-canal treatment failure
Management of non surgical root-canal treatment failureManagement of non surgical root-canal treatment failure
Management of non surgical root-canal treatment failureHamza Tahir
 

What's hot (20)

ATRAUMATIC RESTORATIVE TREATMENT (ART)
ATRAUMATIC RESTORATIVE TREATMENT (ART) ATRAUMATIC RESTORATIVE TREATMENT (ART)
ATRAUMATIC RESTORATIVE TREATMENT (ART)
 
Simplified and modified atraumatic restorative treatment
Simplified and modified atraumatic restorative treatmentSimplified and modified atraumatic restorative treatment
Simplified and modified atraumatic restorative treatment
 
7.recent advances in dentistry
7.recent advances in dentistry7.recent advances in dentistry
7.recent advances in dentistry
 
Conservative and pain free techniques in pediatric dentistry
Conservative and pain free techniques in pediatric dentistryConservative and pain free techniques in pediatric dentistry
Conservative and pain free techniques in pediatric dentistry
 
Treatment plan
Treatment plan  Treatment plan
Treatment plan
 
Pit and fissure sealants
Pit and fissure sealantsPit and fissure sealants
Pit and fissure sealants
 
Diagnosis of dental caries / dental implant courses
Diagnosis of dental caries  / dental implant coursesDiagnosis of dental caries  / dental implant courses
Diagnosis of dental caries / dental implant courses
 
Root Canal Treatment for Patients & Dentist
Root Canal Treatment for Patients & DentistRoot Canal Treatment for Patients & Dentist
Root Canal Treatment for Patients & Dentist
 
Cariesdiagnosis 111107222350-phpapp01
Cariesdiagnosis 111107222350-phpapp01Cariesdiagnosis 111107222350-phpapp01
Cariesdiagnosis 111107222350-phpapp01
 
New Technologies in Caries Diagnosis: The Canary System in Pediatric Practice
New Technologies in Caries Diagnosis: The Canary System in Pediatric PracticeNew Technologies in Caries Diagnosis: The Canary System in Pediatric Practice
New Technologies in Caries Diagnosis: The Canary System in Pediatric Practice
 
Vital pulp therapy technique
Vital pulp therapy techniqueVital pulp therapy technique
Vital pulp therapy technique
 
Orthodontic Assisting Permit Course Day 5 & 6
Orthodontic Assisting Permit Course Day 5 & 6Orthodontic Assisting Permit Course Day 5 & 6
Orthodontic Assisting Permit Course Day 5 & 6
 
Deep caries / dental implant courses
Deep caries / dental implant coursesDeep caries / dental implant courses
Deep caries / dental implant courses
 
CA Orthodontic Assisting Permit Course Day 1-2
CA Orthodontic Assisting Permit Course Day 1-2CA Orthodontic Assisting Permit Course Day 1-2
CA Orthodontic Assisting Permit Course Day 1-2
 
Isolation in endodontic
Isolation in endodonticIsolation in endodontic
Isolation in endodontic
 
ENDODONTIC EMERGENCIES
ENDODONTIC EMERGENCIESENDODONTIC EMERGENCIES
ENDODONTIC EMERGENCIES
 
endodontics
endodonticsendodontics
endodontics
 
Control of moisture
Control of moistureControl of moisture
Control of moisture
 
Management of non surgical root-canal treatment failure
Management of non surgical root-canal treatment failureManagement of non surgical root-canal treatment failure
Management of non surgical root-canal treatment failure
 
Caries diagnosis
Caries diagnosisCaries diagnosis
Caries diagnosis
 

Viewers also liked

The Perio Protect Program
The Perio Protect ProgramThe Perio Protect Program
The Perio Protect ProgramRobert Dmd
 
Etiology of periodontal disease
Etiology of periodontal diseaseEtiology of periodontal disease
Etiology of periodontal diseaseSaeed Bajafar
 
radiographic diagnosis of periodontal disease
radiographic diagnosis of periodontal diseaseradiographic diagnosis of periodontal disease
radiographic diagnosis of periodontal diseaseshabeel pn
 
Perio - The treatment plan
Perio - The treatment planPerio - The treatment plan
Perio - The treatment planSujayaa Rauniyar
 

Viewers also liked (7)

Rei Teeth
Rei TeethRei Teeth
Rei Teeth
 
The Perio Protect Program
The Perio Protect ProgramThe Perio Protect Program
The Perio Protect Program
 
Etiology of periodontal disease
Etiology of periodontal diseaseEtiology of periodontal disease
Etiology of periodontal disease
 
radiographic diagnosis of periodontal disease
radiographic diagnosis of periodontal diseaseradiographic diagnosis of periodontal disease
radiographic diagnosis of periodontal disease
 
Periodontal disease
Periodontal diseasePeriodontal disease
Periodontal disease
 
Periodontal Disease
Periodontal DiseasePeriodontal Disease
Periodontal Disease
 
Perio - The treatment plan
Perio - The treatment planPerio - The treatment plan
Perio - The treatment plan
 

Similar to Perio handout

Hygiene drive philosophy handout
Hygiene drive philosophy handoutHygiene drive philosophy handout
Hygiene drive philosophy handoutcatapulttemp
 
Contamination of microbes and its disinfection in mouthguards used in sports:...
Contamination of microbes and its disinfection in mouthguards used in sports:...Contamination of microbes and its disinfection in mouthguards used in sports:...
Contamination of microbes and its disinfection in mouthguards used in sports:...DrHeena tiwari
 
Deep carious Lesions
Deep carious LesionsDeep carious Lesions
Deep carious LesionsSunny Purohit
 
Lesion Sterilization & Tissue Repair
Lesion Sterilization & Tissue RepairLesion Sterilization & Tissue Repair
Lesion Sterilization & Tissue RepairAhmed Mohsen
 
Diagnosis of dental caries
Diagnosis of dental cariesDiagnosis of dental caries
Diagnosis of dental cariesEkta Garg
 
Treatment of deep caries and pulp exposure
Treatment of deep caries and pulp exposureTreatment of deep caries and pulp exposure
Treatment of deep caries and pulp exposureMohammed_Yazdi
 
final prof lecture on pit and fissure sealant.pptx
final prof lecture on pit and fissure sealant.pptxfinal prof lecture on pit and fissure sealant.pptx
final prof lecture on pit and fissure sealant.pptxpedodonticsdepartmen1
 
Carvalho class ii art_ijpd_2010
Carvalho class ii art_ijpd_2010Carvalho class ii art_ijpd_2010
Carvalho class ii art_ijpd_2010fabiosampa
 
Carvalho class ii art_ijpd_2010
Carvalho class ii art_ijpd_2010Carvalho class ii art_ijpd_2010
Carvalho class ii art_ijpd_2010fabiosampa
 
Dens evaginatus- a problem based approach
Dens evaginatus- a problem based approachDens evaginatus- a problem based approach
Dens evaginatus- a problem based approachAshok Ayer
 
Periodontal implications of bonded vs. removable retainers
Periodontal implications of bonded vs. removable retainersPeriodontal implications of bonded vs. removable retainers
Periodontal implications of bonded vs. removable retainerstoothjockey
 
Radiograpy in pediatric dental patient
Radiograpy in pediatric dental patientRadiograpy in pediatric dental patient
Radiograpy in pediatric dental patientPurna Patel
 
Dentigerous cyst in maxilla in a young girl
Dentigerous cyst in maxilla in a young girlDentigerous cyst in maxilla in a young girl
Dentigerous cyst in maxilla in a young girlMausumi Iqbal
 
Chairside investigations
Chairside investigationsChairside investigations
Chairside investigationsrumelaghoshbds
 

Similar to Perio handout (20)

Hygiene drive philosophy handout
Hygiene drive philosophy handoutHygiene drive philosophy handout
Hygiene drive philosophy handout
 
Contamination of microbes and its disinfection in mouthguards used in sports:...
Contamination of microbes and its disinfection in mouthguards used in sports:...Contamination of microbes and its disinfection in mouthguards used in sports:...
Contamination of microbes and its disinfection in mouthguards used in sports:...
 
Deep carious Lesions
Deep carious LesionsDeep carious Lesions
Deep carious Lesions
 
Lesion Sterilization & Tissue Repair
Lesion Sterilization & Tissue RepairLesion Sterilization & Tissue Repair
Lesion Sterilization & Tissue Repair
 
Mohac Presentation Oct
Mohac Presentation OctMohac Presentation Oct
Mohac Presentation Oct
 
ART.ppt
ART.pptART.ppt
ART.ppt
 
Pit and Fissure Sealants
Pit and Fissure Sealants Pit and Fissure Sealants
Pit and Fissure Sealants
 
Diagnosis of dental caries
Diagnosis of dental cariesDiagnosis of dental caries
Diagnosis of dental caries
 
Treatment of deep caries and pulp exposure
Treatment of deep caries and pulp exposureTreatment of deep caries and pulp exposure
Treatment of deep caries and pulp exposure
 
Avulsion
AvulsionAvulsion
Avulsion
 
final prof lecture on pit and fissure sealant.pptx
final prof lecture on pit and fissure sealant.pptxfinal prof lecture on pit and fissure sealant.pptx
final prof lecture on pit and fissure sealant.pptx
 
jc 1.pptx
jc 1.pptxjc 1.pptx
jc 1.pptx
 
Carvalho class ii art_ijpd_2010
Carvalho class ii art_ijpd_2010Carvalho class ii art_ijpd_2010
Carvalho class ii art_ijpd_2010
 
Carvalho class ii art_ijpd_2010
Carvalho class ii art_ijpd_2010Carvalho class ii art_ijpd_2010
Carvalho class ii art_ijpd_2010
 
ART.ppt
ART.pptART.ppt
ART.ppt
 
Dens evaginatus- a problem based approach
Dens evaginatus- a problem based approachDens evaginatus- a problem based approach
Dens evaginatus- a problem based approach
 
Periodontal implications of bonded vs. removable retainers
Periodontal implications of bonded vs. removable retainersPeriodontal implications of bonded vs. removable retainers
Periodontal implications of bonded vs. removable retainers
 
Radiograpy in pediatric dental patient
Radiograpy in pediatric dental patientRadiograpy in pediatric dental patient
Radiograpy in pediatric dental patient
 
Dentigerous cyst in maxilla in a young girl
Dentigerous cyst in maxilla in a young girlDentigerous cyst in maxilla in a young girl
Dentigerous cyst in maxilla in a young girl
 
Chairside investigations
Chairside investigationsChairside investigations
Chairside investigations
 

Perio handout

  • 1. 9/24/2014 1 The Perio Tray is an FDA cleared, prescription medical device to place solutions of the dentist’s choice into the gingival sulcus or periodontal pocket. Flexible comfortable material for non-invasive delivery. The Perio Tray differs from other trays or mouth guards in that the flexible material is custom formed with specialized seals and extensions for the shape and depth of each pocket so that a gasket-like seal directs and maintains medication in the pocket long enough for medication to have therapeutic effect.
  • 2. 9/24/2014 2 Crevicular flow cleans out the pocket area 40 times per hour under healthy conditions and even more so when the pocket becomes infected With the biofilms attached to the tooth and tissue, these areas become even more resistant to being flushed out
  • 3. 9/24/2014 3 In Vitro PEROXIDE GEL EXPERIMENT Confocal micrograph, untreated control, 3 days in vitro Streptococcus mutans (S. mutans, strain UA 159) biofilm. Confocal micrograph, 3 day in vitro S. mutans biofilm treated for 5 minutes with 1.7% hydrogen peroxide gel. REPORT ON PEROXIDE GEL EXPERIMENT Confocal microscope, S. mutans biofilm treated for 10 minutes with 1.7% hydrogen peroxide. Confocal microscope, S. mutans treated with placebo gel without hydrogen peroxide. 10 minutes BID as maintenance….TID during treatment So When? We recommend place them in prior to your am shower Then brush and do your regimen after Evening time is easy but it can be when you watch TV or really when you want Clean with your toothbrush and water
  • 7. 9/24/2014 7 Fluoride gels with high caries MI Paste with high caries patients or sensitivity Vibramycin for antibiotic usage in the trays when you want alternatives or patient wants alternatives to systemic Truly what you want in customized trays An explorer….a probe….traditional x-rays
  • 8. 9/24/2014 8 Tell me, and I will forget. Show me, and I will remember. Involve me, and I will understand.
  • 9. 9/24/2014 9 It has been suggested that the majority of all plans that go untreated are a direct result of the patient’s lack of understanding.
  • 10. 9/24/2014 10 15 Distal Occlusal Pit 31 Occlusal 18 Occlusal 45 year old… 1 implant crown, 1 crown, 2 restorations on 3 and 14
  • 11. 9/24/2014 11 Visual diagnosis can be highly subjective, Kefley and Holt 1993 Dentin involvement can occur under an intact enamel layer Pitts, 1997 On X-ray, detecting occlusal decay is only when there is dentin penetration by 2-3mm due to the thickness of the x-ray having to penetrate buccal/lingually the thickest portion of the tooth. Bite Wing x-rays can identify inter-proximal lesions earlier because x-rays pass through a narrower part of the tooth yet their sensitivity is still not excellent 40-60% demineralization required to produce image Underestimates size or depth Insufficient to determine activity level Low sensitivity 39% occlusal 50% interproximal Bader et al 2001 70% of overall adolescents experience tooth decay, and far more in lower income families 90% of caries in pit and fissures, and most are in molars Pit and fissures account for 12.5% of the surface area of a tooth and yet this is where 88% of caries occur
  • 12. 9/24/2014 12 The overall annual attack rate for caries for all occlusal first permanent molar surfaces is 5.9% per year, while the approximal surfaces is 1.3% in a representative group of children, seven to fifteen years of age.
  • 13. 9/24/2014 13 Transference of infective S mutans to other sites? 62% sensitivity / low reliability Loesche et al, J Dent Res 1979 Hujoel et al, Caries Res 1995 False positives & false negatives Lussi, Caries Res 1991 Di Disrupts intact i surface f layer, l eliminating li i i potential i l f for reversal Al-Sehaibany showed tug back by an explorer was only 24% diagnostic, meaning that 76% of the time that tug back was present, there was no caries! Ekstrand showed that a sharp explorer tip can damage an early de-mineralized white spot lesion of the enamel by cavitating the surface . 15 Distal Occlusal Pit 31 Occlusal 18 Occlusal 15 and 31 slight sticks…18 no stick…Do you Drill? What is the patient’s caries risk? What does the patient want?
  • 14. 9/24/2014 14 64 31 38 15 Distal Occlusal Pit 31 Occlusal 18 Occlusal Yes……dark stained pits, DRILL or NOT to Drill? 1.0 1.5 2.0 2.5 > 3.0 Sound Enamel Deep Dentin Caries Beginning Enamel C i Deep Enamel Caries Dentin Caries Caries “Doppler Radar” for Caries Detection A Picture is Worth a Thousand Words Analysis of Spectra images Color Scale and Diagnostic Value
  • 15. 9/24/2014 15 D0 – sound fissure system D1‐D2 – Enamel Caries D3‐D4 – Dentin Caries Diss. Madani, 2004 Uni Jena Histological Clinical Analysis Nomenclature of Dental Lesions 15 Distal Occlusal Pit 31 Occlusal 18 Occlusal Color Mapping with Air Techniques and Spectra 15 Distal Occlusal Pit 31 Occlusal 18 Occlusal Infrared Technology…..Continuing to Advance
  • 16. 9/24/2014 16 15 Distal Occlusal Pit 31 Occlusal 18 Occlusal Minimal preparations…well into enamel patient preferred to treat early and avoid shots and future work Treatment with Equia or Low stress bulk fills or Activa •Non stress bearing Class I & II restorations •Deciduous teeth restorations •Geriatric restorations •Base / dentine replacement •Cervical (Class V) restorations •Core build up •Temporary fillings •ART (Atraumatic Restorative Technique)
  • 17. 9/24/2014 17 41 37 No stick Class 1’s Into Dentin Without major occlusal function
  • 19. 9/24/2014 19 Application of EQUIA Coat… ...30-50 μm of penetration in to EQUIA Fil to fill up porosities …increases fracture toughness by 212% …increases flexural strength by 72% …increases flexural fatigue resistance …protects from acid erosion …improves aesthetics …takes long to wear off (6 months or more) EQUIA Coat EQUIA Fil No delamination layer at the interphase EQUIA Coat filling up porosities Depth of penetraition 30- 50μm EQUIA Fil
  • 20. 9/24/2014 20 0,20 0,15 0,10 0,05 0,00 Fuji IX GP EXTRA Fuji IX GP EXTRA + G-COAT PLUS Acid erosion / mm Extra Protection EQUIA Fil EQUIA Fil + EQUIA Coat EQUIA Coat protects from acid erosion, important in High Caries Risk 119999 patients… What happens when Coating wears off? What happens when EQUIA Coat Wears off? The EQUIA restoration undergoes a unique maturation effect attributed to saliva…
  • 21. 9/24/2014 21 1500 1000 500 0 0 20 40 60 80 100 Fluoride release / μg cm-2 Fuji Triage Fuji II LC improve Fuji IX GP FAST Fuji Filling LC EQUIA Fil 202 days Once coating wears off, fluoride recharge cycle is initiated... 120 100 80 60 40 20 0 1day 4 days 7 days 30 days 90 days μm Fuji IX Fuji IX GP Fast GIC have high early wear ……… however due to maturation a long term wear similar to composite I Placed in 1984
  • 25. 9/24/2014 25 Chew the enclosed wax, then spit to the A line Add 1 drop of Reagent 1: Tap the container 15 times Add 4 drops of Reagent 2: Shake until saliva sample turns green Now Dispense 3 scales of saliva into the sample window and wait 15 minutes The red arrow shows the pipet The yellow arrow shows the plastic vehicle we use to mix Drops from Solutions 1 and 2 to mix prior to testing The white plastic testing piece is how we evaluate Strep Mutans results 1. Identification of Antigenic Epitopes in an Alanine-Rich Repeating Region of a Surface Protein Antigen of Streptococcus mutans. N. Okahashi, I. Takahashi, M. Nakai, H. Senpuku, T. Nisizawa and T. Koga. Infection and Immunity, Apr. 1993, vol. 61, N°4:p.1301-1306 2. Rapid and quantitative detection of Streptococcus mutans with species specific monoclonal antibodies. W. Shi, A. Jewett, W.R. Hume. Hybridoma 1998;17:365- 371. 3. Simple and Rapid Immunoassay for the Estimation of Streptococcus mutans in Human Saliva. Y Matsumoto, N. Sugihara, M. Koseki, Y. Maki. Abstract –50th ORCA Congress, July 2-6, 2003, Konstanz, Germany 4. Evaluation of Rapid Screening Test for S. mutans Using Species-specific Monoclonal Antibodies. Y. Maki, N. Sugihara and M. Koseki. Abstract 2077–81st General Session of IADR, June 25-28, 2003, Goteborg, Sweden
  • 26. 9/24/2014 26 After placing Reagent 1 and taping the container 15 times 4 Drops of Reagent 2 are then added to the solution and again mixed until the saliva turns green The solution is THEN placed into the orifice via the pipet and you wait 15 minutes Positive result - Either a faint or clear red line appearing means there are over 500,000 cfu/ml of S. MUTANS. How do you customize their therapy? Cariogenic biofilms thrive in acidic environments. These environments are partially created by eating and drinking certain foods. In order to fight mouth bacteria we need to work on the saliva as well as the surface of the teeth. By working on both aspects we can win the war of caries management.
  • 27. 9/24/2014 27 Real time (15 second ) inexpensive screening test for separating at risk from low risk individuals Cariscreen- Meter that when a swab with pellicle is placed inside , it can read the degree of cariogenicity of the patients biofilm. Depending on the reading, patient can be treated with their proprietary series of rinses. PH of the rinses are in the 8,9, and 10 range. Very basic to change the environment in the oral cavity. (“Measurement of ATP Bioluminescence from Oral Bacteria Contained in Dental Plaque: Basic Sciences and Clinical Assessments for Testing of Caries Risk” by Drs. R. Sauerwein, J. Kimmell, T. Finlayson, S. Fazilat, P. Pellegrini, I. Kasimi, D. Covell, P. Gagneja, J. Engle, K. Kutsch, T. Maier, and C.A. Machida , representing [not in corresponding order], Department of Integrative Biosciences, Academic DMD Program and OCTRI Research Fellowship Program, Department of Pediatric Dentistry, and Department of Orthodontics, School of Dentistry, Oregon Health & Science University, Portland, and Oral Biotech)
  • 28. 9/24/2014 28 What will affect the population of a biofilm Intense heat = 175 degrees F Complete mechanical debrasion Chemotherapeutics What will not affect the population of a biofilm Systemic antibiotics Brushing Flossing Introduction and Science What is Dental Caries: The New Paradigm pH Neutralization: Reduces growth and acid production of cariogenic bacteria, supports healthy oral bacteria growth Antibacterial: Significantly reduces total bacteria levels when high levels are identified Fluoride: Aids in remineralization and inhibits acid production of cariogenic bacteria Xylitol: Reduces growth and acid production of cariogenic bacteria Nano HA (Ca PO4): Aids in remineralization with nano particles of hydroxyapatite Targeted Therapy What is CTx? How the CTx Guide Applies to Products Number of agents equals CTx Score
  • 29. 9/24/2014 29 CTx4 Treatment Rinse pH Neutralization Antibacterial (.2% sodium hypochlorite) Fluoride (0.05%) Xylitol (Greater than 10%) 3 Main Products CTx4 Gel 5000 pH Neutralization Fluoride (1.1% Neutral Sodium Fluoride) HA Nano (Ca PO4) Xylitol (Greater than 10%) CTx3 Rinse pH Neutralization Fluoride (0.05%) Xylitol (Greater than 10%)
  • 30. 9/24/2014 30 † Distributed exclusively by dental professionals † Extra-strength blend of ProBiora3® crowds out harmful bacteria around teeth and gums † Use once daily for 90 days after a professional cleaning † Extends the benefits of a dental prophy ProBiora3 – Ingredient in branded products
  • 31. 9/24/2014 31 † For companion pets † Freshens breath, cleans and whitens teeth †Tasteless, odorless powder † Strains weighted for gum health †ProBiora3 is the most comprehensive oral care probiotic technology available †Developed from research into dental caries and periodontal disease †Blend of 3 naturally occurring Streptococcal strains – S. oralis, S. uberis, S. rattus †ProBiora3 promotes: †dental and periodontal health †whiter teeth †fresher breath Probiora3: Product Overview ProBiora3 Marketed as Food Ingredient †Self-affirmed GRAS status †ProBiora3 is safe and effective 15 peer-reviewed publicationsProBiora3
  • 32. 9/24/2014 32 Competitive Advantage †Strong scientific basis for safety and efficacy †ProBiora3 is the first and only comprehensive oral care probiotic on the market ProBiora3 – Competitive Advantage The ProBiora3 contains a formulation of beneficial bacteria, found in naturally healthy human mouths. S. oralis KJ3 S. uberis KJ2 S. rattus JH145 S.rattus JH145 is a unique strain of streptococcus that does not produce lactic acid, and has been shown to successfully compete for nutrients and space on tooth surfaces with the native strain of streptococcus that produces lactic acid. ProBiora3 – How it works A spontaneous lactacte dehydrogenase deficient mutant of Streptococcus rattus for use as a probiotic in the prevention of dental caries. Hillman JD, McDonell E, Cramm T, Hillman CH, Zahradnik RT. Journal of Applied Microbiology 2009 Nov;107(5):1551-8. Epub 2009 Apr 24) ProBiora3 – How it works
  • 33. 9/24/2014 33 ProBiora3 – How it works
  • 34. 9/24/2014 34 Remin Pro supports remineralization. Remin Pro strengthens teeth. Remin Pro smooths tooth surfaces. Remin Pro neutralizes acids. Remin Pro desensitizes. Remin Pro tastes good. Contains all ingredients of natural tooth substance... Calcium ions Ph h i hydroxyapatite - Phosphate ions - Water/fluorides y yp [Ca5(PO4)3OH] • Remin Pro is ideal for protective tooth care Three Key Ingredients: - Fluoride (1450 ppm) - Hydroxyapatite (Calcium and Phosphate) = natural tooth substance - Xylitol (non-sugar sweetener with cariostatic effect) Natural tooth substance consists largely of hydroxyapatite (calcium and phosphate). It fills superficial enamel lesions and the tiniest irregularities that arise from erosion. Remin Pro adheres to the tooth substance and protects the tooth against demineralization and erosion. Smooths the surface by sealing dentin tubules. A smooth surface impairs the adhesion of bacterial plaque.
  • 35. 9/24/2014 35 Reduction of caries formation Sealing of tooth surfaces, desensitization Repair of incipient caries (remineralization) Delay of plaque formation and smoother surfaces Teeth whitening and gloss increase Kani T, Kani M, Isozaki A, Kato H, Fukuoka Y, Ohashi T, Tokumoto T: The effect of apatitecontaining dentifrices on artificial caries lesions. I Dent Health 38, 364–366 (1988). Okashi T, Kani T, Isozaki A, Nishida A, Shintani H, Tokumoto T, Ishizu E, Kuwahara Y, Kani, M: Remineralization of artificial caries lesions by Hydroxyapatite. I Dent Health 41, 214 – 223 (1991). Nishio M, Kawamata H, Fujita K, Ishizaki T, Hayman R, Ikemi T: A new enamel restoring agent for use after PMTC. Posterpresentation 82nd General Session & Exhibition of the IADR / March 2004. Kawamata H, Nishio M, Fujita K, Ishizaki T, Hayman R, Ikemi T: Posterpresentation 82nd General Session & Exhibition of the IADR / March 2004. Yamagishi K, Onuma K, Suzuki T, Okada F, Tagami J, Otsuki M, Senawangse P: Materials chemistry: A synthetic enamel for rapid tooth repair. Nature. 2005 Feb 24; 433 (7028): 819. Sugar substitute comes from the Xylitol portion of a plant. Cannot be metabolized by acid producing cariogenic bacteria. Excreted as whole molecule. Natural population shift to helpful bacteria. 6- 10 grams per day. Potentially toxic to dogs. Xlear, Dr. John’s candies, Xylitol USA
  • 36. 9/24/2014 36 The Glucose is absorbed by the Strep Mutan the Glucose then goes through a metabolic breakdown and ultimately gets excreted as acids and thus the pathway to caries Xylitol gets absorbed by the Strep Mutans, occupies or keeps the Strep Mutans busy but DOES NOT get broken down, and is excreted in whole… After short and long-term use, xylitol significantly reduces the amount of Strep Mutans in plaque and saliva. The growth of lactobacilli is reduced during long-term use of xylitol. Xylitol shows effects which promote tooth remineralization. Xylitol actively prevents acid production in dental plaque (enamel dissolves when the pH decreases below 5.7). Regular xylitol consumption reduces the adhesive property of plaque, making it easier to brush off. http://www.ncbi.nlm.nih.gov/pubmed/12693818 An overview of studies about xylitol and dental caries Mäkinen K.K. The rocky road of xylitol to its clinical application. J. Dent. Res. 2000; 79: 1352. Lynch H, Milgrom P. Department of Dental Public Health Sciences, Northwest/Alaska Center to Reduce Oral Health Disparities, University of Washington, Seattle 98195-7475, USA. Xylitol and dental caries: an overview for clinicians. J Calif Dent Assoc. 2003 Mar;31(3):205-9. K.K. Mäkinen, K.P.Isotupa, T. Kivilompolo, P.L. Mäkinen, J.Toivanen, E. Söderling. Comparison of Erythritol and Xylitol Saliva Stimulants in the Control of Dental Plaque and Mutans Streptococci. Caries Research 2001;35:129-135. Kandelman D., Gagnon G. A 24-month clinical study of the incidence and progression of dental caries in relation to consumption of chewing gum containing xylitol in school preventive programs. J. Dent. Res. 1990; 69: 1771. Kauko K. Mäkinen, MS, Phd, Kauko P. Isotupa, DDS, Taina Kivilompolo, RDH, Pirkko-Liisa Mäkinen, MS, PhD, Satu Murtomaa, DDS, Juhani Petäjä, DDS, Jukka Toivanen, DDS, Eva Söderling, MS, PhD. The effect of polyol-combinant saliva stimulants on S. mutans levels in the plaque and saliva of patients with mental retardation. Special Care Dentistry 2002; 22(5): 187-193. Isotupa K.P., Gunn S., Chen C.Y., Lopatin D., Mäkinen K.K. Effect of polyol gums on dental plaque in orthodontic patients Am. J. Orthod. Dentofac. Orthop. 1995; 107: 497. Mäkinen K.K., Bennett C.A., Hujoel P.P., Isokangas P., Isotupa K.P., Pape H.R. Jr., Mäkinen P.-L. Xylitol gums and caries rates: A 40-month cohort study. J. Dent. Res. 1995; 74: 1904. Autio J.T., Courts F.J. Acceptance of the xylitol chewing gum regiment by preschool children and teachers in a Head Start program: a pilot study. Pediatr. Dent. 2001; 23:71. Autio J.T. Effect of xylitol chewing gum on salivary streptococcus mutans in preschool children. ASDC J. Dent. Child. 2002; 69: 81-6, 13. Alanen, P., Holsti, M.-L., Pienihäkkinen, K. (2000) Sealants and xylitol chewing gum are equal in caries prevention. Acta Odontol. Scand. 58:279- 284. Caufield, P.W., Cutter, G.R., Dasanayake, A.P. (1993) Initial acquisition of mutans streptococci by infants: evidence for a discrete window of infectivity. J. Dent. Res. 72:37-45. Trahan, L. (1995) Xylitol: a review of its action on mutans streptococci and dental plaque – its significance. Int. Dent. J. 45:77-92. Scheinin, A., Mäkinen, K.K. (1971) The effect of various sugars on the formation and chemical composition of dental plaque. Int. Dent. J. 21:302- 321. Scheinin, A., Mäkinen, K.K. (1972) Effect of sugars and sugar mixtures on dental plaque. Acta Odontol. Scand. 30:235-257. Scheinin, A., Mäkinen, K.K. (1975) Turku Sugar Studies I-XXI. Acta Odontol. Scand. 33 (Suppl. 70):1-349. Isokangas, P. (1987) Xylitol chewing gum in caries prevention. Academic Dissertation, University of Turku. Isokangas, P., Mäkinen, K.K., Tiekso, J., Alanen, P. (1993) Long-term effect of xylitol chewing gum in the prevention of dental caries: a follow-up 5 years after termination of a prevention program. Caries Res. 27:495-498. Mäkinen, K.K., Bennett, C.A., Hujoel, P.P., Isokangas, P.J., Isotupa, K.P., Pape, H.R., Jr., Mäkinen, P.-L. (1995) Xylitol chewing gums and caries rates: a 40-month cohort study. J. Dent. Res. 74:1904-1913. Hujoel, P.P., Mäkinen, K.K., Bennett, C.A., Isotupa, K.P., Isokangas, P.J., Allen, P., Mäkinen, P.-L. (1999) The optimum time to initiate habitual xylitol gum-chewing for obtaining long-term caries prevention. J. Dent. Res. 78:797-803.
  • 37. 9/24/2014 37 • With the finger • Using a soft toothbrush • Plaed in a tray Recaldent- Casein phosphopeptide (CPP)- amorphous calcium phosphate (ACP) Unique chemical in that it is substantive with a 3 hour half life MI Paste +----5 Calcium,3 phosphate,1 Fluoride—Ideal biochemical ratio to drive remineralization. Made from a milk protein, so contraindicated in patients with true milk allergy. OK for patients who are lactose intolerant Caution with young children. • Two Components 1. ACP:Amorphous Calcium Phosphate 2. CPP: Casein Phosphopeptide – natural occurring molecule • Milk Protein • Protects the ACP component • Delivery vehicle • Very sticky Functions: CPP-ACP attached to strep mutans making them non-viable 252 – Remineralization – Desensitization What is RecaldentTM (CPP-ACP)?
  • 38. 9/24/2014 38 Molecular model of the CPP-ACP complex. Casein phosphopeptide (CPP) is a milk derived protein able to bind calcium and phosphate ions and stabilize them as Amorphous Calcium Phosphate (ACP). ACP alone does not remain stable enough for effective long-term remineralization* 253 g *Cross KJ, Huq NL, Reynolds EC. Casein phosphopeptides in oral health—chemistry and clinical applications. Curr Pharm Des. 2007;13:793-800. CPP-ACP is added to the oral cavity. The ‘sticky’ CPP part binds readily to enamel, pellicle, plaque and soft tissue 254 H+ H+ H+ H+ Under acidic conditions, RecaldentTM is able to release Calcium and Phosphate ions 255
  • 39. 9/24/2014 39 The free Ca and P ions move out of the CPP, enter the 256 enamel rods and reform into apatite crystals • Helps to maintain a state of super-saturation of Calcium and Phosphate ions on the tooth surface • RecaldentTM Depressing enamel demineralization is the ‘fluid enamel’ • Buffers plaque pH • which The longer CPP-restores ACP is maintained mineral in the mouth, the balance more effective the result 257 Improved Plaque Uptake and Enamel Remineralization by Fluoride with CPP-ACPE C Reynolds, N.J. COCHRANE, P. SHEN, F. CAI, G.D. WALKER, M.V. MORGAN, and C. REYNOLDS, Cooperative Research Centre for Oral Health Science, School of Dental Science, The University of Melbourne, Victoria, Australia Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) has been shown to slow the progression of caries and to remineralize enamel subsurface lesions. Objectives: The aim of the studies was to determine the ability of CPP-ACP to increase the incorporation of fluoride into supragingival plaque and to promote enamel remineralization in situ with acid resistant mineral. Methods: Randomized, double-blind cross-over studies were designed involving three mouthrinses and five toothpastes as follows: Mouthrinses (i) 2% CPP-ACP, (ii) 2% CPP-ACP plus 450 ppm F and (iii) 450 ppm F; and Toothpastes: (i) placebo, (ii) 1100 ppm F, (iii) 2800 ppm F, (iv) 2% CPP-ACP and (v) 2% CPP-ACP plus 1100 ppm F. The mouthrinses (15 ml) were used for 60 s, three times per day for 5 d and supragingival plaque collected and analyzed for F content. The toothpastes (1 g) were added to 4 ml water to form a slurry and used for 60 s four times per day for 14 days in an in situ remineralization model. Results: The addition of 2% CPP-ACP to the 450 ppm F rinse significantly increased the incorporation of fluoride ions into plaque where the plaque fluoride level (33.0±17.6 nmol/mg dry wt) was over double that obtained with the fluoride-only rinse (14.4±6.7 nmol/mg dry wt). Fluoride in the toothpaste slurry produced a dose-response related remineralization of subsurface enamel lesions. The toothpaste containing 2% CPP-ACP produced a level of remineralization (13.5%±1.5%) similar to the 2800 ppm F paste (15.5%±2.4%) and the paste containing 2% CPP-ACP plus 1100 ppm F was superior (21.0%±5.9%) to all other formulations in enamel lesion remineralization. Acid challenge of the remineralized lesions showed that the CPP-ACP/F mineralized lesions were relatively acid resistant. Conclusion: CPP-ACP promotes the incorporation of fluoride into plaque and sub-surface enamel producing effects superior to fluoride alone.
  • 40. 9/24/2014 40 Reduces sensitivity Decreases or eliminates white spots Assists in Caries management Children with cavitations and positive caries tests Children undergoing orthodontics. Adults with positive caries tests and cavitations People with sensitive teeth People taking medications that alter quantity and quality of saliva People undergoing a lot of restorative Dentistry.
  • 41. 9/24/2014 41 Documentation and progession control. 262 263 Before treatment (cleaning) After treatment (cleaning)
  • 42. 9/24/2014 42 HCL Acid etch of all the teeth. Prema- Premier Opalustre- Ultradent Brown & white enamel demineralization PREMA® is applied using 10:1 reduction angle – slow speed After a 14-day take home whitening treatment Immediately after PREMA® treatment Approximately 15 seconds per tooth- open the enamel pores. Following this a 37% phosphoric acid is applied to the teeth for 30 seconds. After rinsing it off, Remin Pro( MI Paste), is applied in the office for 5 minutes. This can be achieved with a gloved finger and leaving the cream in place or with a gloved finger and then placing the tray over the cream. Patient is sent home and told to wear the trays as much as possible daily with the cream in place.
  • 43. 9/24/2014 43 37% Phosphoric acid for 30 seconds followed by 5 minutes of therapeutic cream. This is done weekly till 50-60% remineralization is seen. Now Pola Night (10% for kids or Pola Day CP 35% for adults) is added to the protocol daily for ten days. Continue etch/cream protocol till desired result is achieved
  • 46. 9/24/2014 46 Futurabond® U The Dual-Cure Universal Adhesive! .
  • 47. 9/24/2014 47 self-etch selective-etch total-etch
  • 49. 9/24/2014 49 MI Paste in a bleaching tray for as long as she can wear it everyday. (Homework) Pola night — 15% started at week 8 for 2 weeks. Continued with MI Paste