Bayyinatul Muchtaromah's research developed a new herbal jamu product called "Subur Kandungan Madura" using a nanotechnology approach. The jamu contains extracts of garlic, temu mangga, and jeringau encapsulated in chitosan nanoparticles. Nanoparticle characterization showed particles around 438 nm in size with good uniformity. In vitro tests found the jamu demonstrated antioxidant effects and immunomodulation potential. The research concludes the jamu shows potential for developing a fertility-enhancing product using a nanotechnology delivery system for herbal extracts.

1. DEVELOPMENT OF
JAMU “SUBUR KANDUNGAN MADURA”
BASED ON NANOTECHNOLOGY
(New Biology Approach For New Normal)
BAYYINATUL MUCHTAROMAH
MAGISTER BIOLOGI
FAKULTAS SAINS DAN TEKNOLOGI
UIN MAULANA MALIK IBRAHIM MALANG
2020

2. ْ‫ن‬ِ‫م‬ ‫ا‬َ‫ه‬‫ي‬ِ‫ف‬ ‫َا‬‫ن‬ْ‫ت‬َ‫ب‬ْ‫ن‬َ‫أ‬ ْ‫م‬َ‫ك‬ ِ
‫ض‬ ْ‫ر‬َ ْ
‫اْل‬ ‫ى‬َ‫ل‬ِ‫إ‬ ‫ا‬ ْ‫و‬ َ‫ر‬َ‫ي‬ ْ‫م‬َ‫ل‬ َ‫و‬َ‫أ‬
ٍ‫يم‬ ِ
‫ر‬َ‫ك‬ ٍ‫ج‬ ْ‫و‬ َ‫ز‬ ِ‫ل‬ُ‫ك‬
‫ي‬ِ‫ن‬ِ‫م‬ْ‫ؤ‬ُ‫م‬ ْ‫م‬ُ‫ه‬ُ‫ر‬َ‫ث‬ْ‫ك‬َ‫أ‬ َ‫ان‬َ‫ك‬ ‫ا‬َ‫م‬ َ‫و‬ ۖ ً‫ة‬َ‫ي‬ َ
‫َل‬ َ‫ك‬ِ‫ل‬ََٰ‫ذ‬ ‫ي‬ِ‫ف‬ َّ‫ن‬ِ‫إ‬
َ‫ن‬
ُ‫م‬‫ي‬ ِ‫ح‬َّ‫الر‬ ُ‫يز‬ ِ
‫ز‬َ‫ع‬ْ‫ال‬ َ‫و‬ُ‫ه‬َ‫ل‬ َ‫َّك‬‫ب‬ َ‫ر‬ َّ‫ن‬ِ‫إ‬ َ‫و‬
And do they not look at the earth, how ma
ny We grow on earth a variety of good her
bs? Verily in that is a sign of Allah. And m
ost of them do not believe, and verily the
Lord is He that is the
ASY-SYU’ARA 7-9
BACKGROUND

3. Research Background
Terapi obat sintetik (Usadi & Merriam, 2015), in vitro
fertilization dan teknologi reproduksi
Pengobatan Infertilitas
Memiliki potensi meningkatkan kesuburan adalah
bawang putih (Allium sativum), jeringau (Acorus calamus)dan
temu mangga (Curcuma mangga)
Obat Herbal
Infertilitas dialami oleh sekitar 10-15% pasangan usia
subur di dunia dan lebih banyak dialami wanita
Infertilitas

4. Research Background
Memudahkan ekstrak terserap dalam plasma darah dan lebih
efektif dalam mencapai target obat itu sendiri
Nanopartikel
Partikel herbal lebih mudah menyebar dalam sirkulasi
darah, pencapain pada target pengobatan lebih akurat
Penghantaran Obat
Dibutuhkan dosis yang besar karena Ekstrak tumbuhan
mempunyai kelarutan yang rendah
Kendala Jamu Herbal

5. “Jamu Subur Kandungan”
15% Garlic
From
packaging
labels
15% Temu
mangga
12 % Jeringau
Have function : fertilizing the uterus, helping to strengthen the uterus
muscles, prevent miscarriage, help fertilize the uterus, assist fertilization,
help nourish the body, help hormonal balance and strengthen the fetal
attachment
Prophet Muhammad ` said: “Marry the kind and fertile women who will give birth to many children for I shall take
pride in the great numbers of my ummah” (Nation) [Sahih Abi Dawood Hadith No 2050, Maktibt Al Maaref, Riyadh].

6. “Jamu Subur Kandungan”
Garlic : alkaloids, steroids, saponins, tannins, & glycosidesas: aphrodisiacs antibacterial, antiviral, antifungal antiprotozoal
immunomodulator (Arekemase,et al.,2013).
Temu Mangga
antioxidant compounds, including chalcones, flavonoids, flavone, flavanone :
Suppress free radical, inhibits peroxide formation during lipid oxidation,
antiallergic (Yuandani et al, 2011). antimicrobials such as C. albicans, S. aureus
and E. coli (Jawetz et al., 2005) and immunomodulators
Jeringau
Contains phenolic compounds, amino acids, proteins, carbohydrates, alkaloids,
flavonoids, terpenoids, steroids, saponins and tannins. Function: as a food and
beverage flavors, aromatherapy, cosmetics, fragrance ingredients, anti-
inflammatory, antioxidant, antiseptic, antibacterial, antifungal and medicine for
various types of diseases in humans and animals (Hartati, 2012).

7. Unpleasen taste and have low solubility in the digestive tract
resulting in difficult entry into the blood plasma and need large
dose (Marczylo, et al., 2007)
7
PROBLE
M

8. Herbal based on
Nanotechnology
• 1 Encapsulation Technology
Causes the extracts more accurately in achieving the target
(Poulain&Nakache, 1998)
• 2 Chitosan
Widely used as a drug coating for the purpose of optimizing drug
absorption in target cells. Desai & Park (2005)
• 3 Ionic gelation method
One method that can be used for the manufacture of
nanoparticles
8
In Islam or quranic word, a very
small particle of matter is
called “zerre”. With this verse,
the attention is drawn to the
small particles of matter,
including nanoparticles. Allah
said: Not even an atom’s (zerre)
weight in the heavens and the
earth, or something smaller or
greater than it is hidden from
Him, but all are in a clear
record. (Saba 34:3)

9. Phytochemical screening, antioxidant activity,
antifungal and antibacterial of nanoparticles of
A. sativum, C.mangga and A. calamus coated
chitosan invitro
Third Year
ROADMAP AND PHASE OF RESEARCH
9
First Year Characterization nanoparticles of A. sativum, C. mangga and
A. calamus coated chitosan include morphology, particle size,
functional groups, and degree of crystallinity of nanoparticles
obtained
Second Year
Dosage optimization test in vivo (in
experimental animals)

10. 6/9/2023 10
Chitosan 0.5% in 0.5% glacial acetic acid 100 ml was put in a beaker glass as much as 100 ml.
It is sonicated with a frequency of 20 kHz, an amplitude of 80% with a combination of volume and duration of
sonication 90, 120, 150 minutes.
Inserted 0.1 g of extract and stirred using a homogenizer with a speed of 3000 rpm for 30 minutes
Tween 80 was added as much as 1 ml and stirred using a 1000 rpm homogenizer for 10 minutes
Take 4 ml sample for PSA examination, the rest is centrifuged 5000 rpm 90 minutes, pellets
are put in a 24 h deep freezer, incubated 3 h, after drying is crushed for PSA, FITR, XRD SEM
test
Data Analysis
Added 0.5% TPP solution as much as 20 ml and homogenized 10.000 rpm for 90 minutes
Nanoparticle extract
coated chitosan

11. Result and Discussion
Nanoparticle yield by ionic gelation method
184.45
19
55.8
29
16.5
117.25
15 14.4 25
39.3
203.5
4
37.9 43
22
0
50
100
150
200
250
Chitosan Temu Mangga coated
chitosan
Garlic coated chitosan Jeringau coated chitosan Combination coated
chitosan
nanoparticle
yield
(mg)
90" 120" 150"

12. Particle characterization using PSA
12
713
673
724
1159
700
578
617
560
1001
438
669
507
343
607
508
0
200
400
600
800
1000
1200
1400
Chitosan Temu Mangga coated
chitosan
Garlic coated chitosan Jeringau coated chitosan Combination coated
chitosan
Peak
Summary
diameter
(nm)
Nanoparticle size using PSA
90" 120" 150"

13. Particle characterization garlic, temu manga and jeringau
combination using PSA
The Power of PowerPoint | thepopp.com 13

14. 6/9/2023 14
No 90 minute 120 minute 150 minute
1. Viscosity (cp) 0.845 cp 1.247 cp 0.849 cp
2. Mean size (MI) (nm) 805 517 528
3. Dispersity Index (PDI) 0.238 0.1464 0.074
4. Highes Peak (nm) 478 438 508
PSA of Nanoparticle Combination of Garlic, Temu Mangga, Jeringau
Coated Chitosan

15. Particle characterization using FTIR
The Power of PowerPoint | thepopp.com 15
Functional
Groups
Absorption Band cm-1
Sonicated Chitosan
at 90”
Sonicated Chitosan at
120”
Sonicated Chitosan at
150”
Literature
O-H stretch 3429 3447 3466 4000-3200
C-H stretch 2930 - - 3000-2800
N-H 1648 1651 1652 1650-1580
C-NH3 1540 1541 1541 1550-1500
CH Residual 1384 1384 1384 1400-1000
P=O 1384 1384 1384 1400-1000
-C-OH 1090 1095 1089 1125-1085
C-H 891 887 834 960-730

16. The morphology of the combination of garlic, temu mangga , and jeringau nanoparticle
with SEM sonicated at 90 min (A), 120 min (B), and 150 min (C)
The Power of PowerPoint | thepopp.com 16

17. The nanoparticle characterization using XRD. A) Chitosan nanoparticle B) Nanoparticle of
garlic, temu mangga & jeringau combination
The Power of PowerPoint | thepopp.com

18. UJI FITOKIMIA
Golongan
senyawa
Ekstrak
Temu
Mangga
Ekstrak
Jeringau
Ekstrak
Bawang
Putih
Nano
Temu
mangga
Nano
Jeringau
Nano
Bawang
putih
Nano
Bawang
putih
Nano
kombinasi
Alkaloid - - - + + + + +
Flavonoid - - - - - - - -
Triterpenoid - - - - - - - -
Steroid - - - - - - - -
Saponin - - + + + + + +
Tanin + + - + + + + +

19. Kromatogram Nanopartikel Ekstrak Kombinasi

20. Total senyawa hasil lcms
73
38
123
0
20
40
60
80
100
120
140
Bawang Putih Jeringau Kombinasi
Total senyawa

21. Bioavailibility dan protein target
1. p-coumaric acid
2. caffeic acid
3. ferulic acid
4. sinapic acid

22. Analisis Potensi Obat Oral Berdasarkan Pendekatan Lipinski
No Reseptor Terget Senyawa
1 Family AG reseptor
 2,4-dimethylfuran,
 methyl propyl disulphide,
 diallyl trisulfide,
 allyl trisulfide,
 calamusenone,
 1-(2,4,5-
trimethoxyphenyl)propan-2-
one,
 isosinomenine,
 zerumin B
2 Lyase (Carbon anhydrase 1)
 diallyl sulphide
 2,4,5-trimethoxybenzaldehyde
3 Protease
 methyl allyl disulphide,
 diallyl disulphide,
 epishyobunone,
 isoacolamone
 agigenin
4 Hydrolase
 dimethyl trisulfide,
 2-vinyl-4-H-1,3-dithiin,
 Allicin,
 di-(2-propenyl)trisulfide,
 3,5-diethyl-1,2,4-trithiolane,
 diallyl tetrasulfide
 preisocalamendiol

23. Analisis Potensi Obat Oral Berdasarkan Pendekatan Lipinski
No Reseptor Terget Senyawa
5 Oxydoreductase  (-) S-allyl-L-cysteine,
 methylisoeugenol,
 alliin,
 aceteugenol,
 isoasarone,
 β-asarone,
 acolamone,
 acoragermacrone,
 acorenone,
 calacone,
 isoacoramone,
 calamendiol,
 isocalamendiol,
 ajoene
 acoric acid
6 Nuclear receptor (subfamily 1)  camphor,
 dipropyl disulphide
 isoshyobunone.
7 Cytochrome p450  acorenol,
 acoronene
 Acorone
8 Calcium channel auxiliary subunit
alpha2delta
 methiin,
 cycloalliin,
 Isoalliin
 methylselenocysteine.

24. Quercetin
Tyrosine-protein
kinase receptor FLT3
Insulin-like growth
factor I receptor
Cytochrome P450
19A1
Bioavailibility dan Protein Target

25. Cytochrome P450 19A1 and Estrogen

26. Tyrosine-Protein Kinase Receptor FLT3

27. Aktivitas Antioksidan
15.68
12.93
0
2
4
6
8
10
12
14
16
18
Ramuan Kombinasi
Nilai IC50
Nanopartikel Ekstrak

28. Flyer produk STIMUNO di Kamboja (gambar sebelah kiri) dan
penampilan display produk STIMUNO di apotek chain-
Mercury di Manila.

29. The immunomodulation profile of A.sativum, C. mangga, and A.calamus extract on the relative
number of CD4+ TNFα+ and CD11b+ TNFα+

30. CONCLUSION
6/9/2023 30
Chitosan- nanoparticles of garlic (A. sativum), temu mangga (C.
mangga) and Jeringau (A. calamus) was successfully produced
with ionic gelation method. (Particle size : 438 nm (120’),
Uniformity rate of 0.14 (very good), FTIR results indicate several
important functional group.

31. Thank You
‫جزيال‬ ‫شكرا‬