Three students, Habib, Vikki and Claire, in Bio 200 ran their samples of HindIII cut plasmid DNA side by side on the same gel and got the results shown below. Habibs bands are not as bright as Claires and Vikki has no DNA. Their lab instructor has asked them to consider where in the mini-prep procedure Habib and Vikki may have gone wrong. From the list of mistakes below, choose one mistake that could account for Habibs results and one mistake that could account for Vikkis results. Explain why they would lead to less bright bands for Habib and no DNA for Vikki. Using 1.5 ml of E.coli instead of 3 ml Not adding lysis buffer Washing with PE first and then PB Not adding enough HIndIII Not adding enough SyberSafe Vortexing their tubes when they werent supposed to Transferring the white pellet to the filter cartridge Habib Carly Vikki.

1. Three students, Habib, Vikki and Claire, in Bio 200 ran their samples of HindIII cut plasmid
DNA side by side on the same gel and got the results shown below. Habibs bands are not as
bright as Claires and Vikki has no DNA. Their lab instructor has asked them to consider where
in the mini-prep procedure Habib and Vikki may have gone wrong. From the list of mistakes
below, choose one mistake that could account for Habibs results and one mistake that could
account for Vikkis results. Explain why they would lead to less bright bands for Habib and no
DNA for Vikki.
Using 1.5 ml of E.coli instead of 3 ml
Not adding lysis buffer
Washing with PE first and then PB
Not adding enough HIndIII
Not adding enough SyberSafe
Vortexing their tubes when they werent supposed to
Transferring the white pellet to the filter cartridge Habib Carly Vikki