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B Reacciones alostricas Hill Plot 1 Una protena tiene.pdf

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B. Reacciones alostricas (Hill Plot) 1. Una protena tiene una afinidad de unin por su ligando de Ka = 2 x 105 M-1 a pH 5,0 y 25o C. (i) A qu concentracin del ligando se une la mitad de la protena? (ii) Qu fraccin de la protena se une a una concentracin de ligando de 1,25 M? (iii) A qu concentracin de ligando se unira el 80% de la protena? (iv) Cuando el pH se eleva a 6,5, la Kd aumenta a 20 M. La unin es ms fuerte o ms dbil a este pH en comparacin con 5,0? Explica tu respuesta. (v) Qu residuo(s) de aminocido podran ser responsables del cambio observado en la afinidad de unin con el cambio en el pH?.

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B. Reacciones alostricas (Hill Plot) 1. Una protena tiene una afinidad de unin por su ligando de Ka = 2 x 105 M-1 a pH 5,0 y 25o C. (i) A qu concentracin del ligando se une la mitad de la protena? (ii) Qu fraccin de la protena se une a una concentracin de ligando de 1,25 M? (iii) A qu concentracin de ligando se unira el 80% de la protena? (iv) Cuando el pH se eleva a 6,5, la Kd aumenta a 20 M. La unin es ms fuerte o ms dbil a este pH en comparacin con 5,0? Explica tu respuesta. (v) Qu residuo(s) de aminocido podran ser responsables del cambio observado en la afinidad de unin con el cambio en el pH?

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