This document provides information about the enzyme peroxidase and an upcoming lab experiment on the effects of pH on peroxidase activity. It discusses that the experiment will investigate how varying the pH between 4, 7, and 10 impacts the function of peroxidase enzymes. Students are instructed on the experimental procedure, which involves measuring the reaction rate of peroxidase catalyzing guaiacol and hydrogen peroxide to form a brown product. The data collected will be graphed to determine initial reaction rates and assess how pH affects peroxidase activity.

1. Enzymes: Form and Function
Catalytic proteins
Lower activation energy of reaction
Combine or break apart substrates
Not used up in reaction
End in –ase
Lactase metabolizes lactose
DNA Polymerase – polymerize nucleotides
Substrate to product
https://www.khanacademy.org/test-
prep/mcat/biomolecules/enzyme-structure-and-
function/a/enzyme-structure-and-function
1
Energy of activation with enzyme
Energy needed to run reaction without an enzyme
Temp & pH
Changes in environmental conditions will alter enzyme

2. structure, and therefore alter its function
Enzyme activity measured by
Initial reaction velocity (IRV)
Amount of product per time
this is what you’re doing in today’s experiment
Or could use amount of product per degree (for a temperature
graph)
what is shown in the graph above
IRV = (slope of the graph)
Reaction you will run:
the reaction goes from clear to brown
peroxidase is not used up in reaction
guaiacol gets oxidized; hydrogen peroxide gets reduced
This is a redox reaction; Think about how rust turns a different
color when iron is oxidized
Baseline: Filtered water – are the enzyme concentrations too
weak/strong?
Question: how does pH impact the function of peroxidase?
Hypothesis: ______________________________________ _
Experiment: try at pH of 4, 7, and 10
guaiacol (clear) + H2O2 tetraguaiacol (brown)
+ H20
peroxidase
Experiment
USE CORRECT PIPETTE FOR EACH CHEMICAL

3. Make sure to only take out 300 microliters of Guaiacol
100 microliters to .1 milliliter
Get Guaiacol from me
Prepare your blank to tare the colorimeter – fill cuvette 2/3 full
and make sure the clear side of cuvette is perpendicular to the
colorimeter’s light
Don’t touch the clear sides (fingerprints are a problem).
Micropipettes:
Don’t turn upside down. 1st resistance to extract chemical; 2nd
resistance to eject chemical. Pay attention to units on pipette.
Don’t reuse tips.
Before adding enzyme solution to test, have all of your supplies
ready:
your colorimeter tared and ready to go
Guaiacol, pH buffer, and H202 in a test tube
stopwatch
Once enzyme is added, quickly pour into cuvette and place in
colorimeter, recording every 15 sec.
Input data into excel sheet for all of class to record
Calculate IRV using greatest difference between two adjacent
points
You will make a line graph of our data using Excel (there will
be 4 lines on graph)
BRING THIS GRAPH NEXT WEEK TO CHECK
CORRECTNESS!
We will cover components of the lab report following your lab
practical next week
You will have access to sample lab reports, rubrics, instructions

4. Topic: Effects of pH on Peroxidase
upcoming due dates:
Materials and Methods – due March 15th 11:59pm on Canvas
Literature Review – due April 1st 11:59pm on Canvas
Introduction – due April 8th 11:59pm on Canvas
entire lab report – due April 22nd 11:59pm on Canvas
Lab Report – due March 25th
11:59pm on Canvas using Turnitin
Next week: BSC110L LAB PRACTICAL
covers labs: Topics 1-4 (scientific method, biomolecules,
microscopes and cells,
enzymes)
worth 20% of your grade!
DO NOT arrive late
no electronics on your person (phones, ear buds,
smartwatches in your bag)
Written Exam: 40-45 questions on written portion
55 questions, 2 points each
One or two stations for practical applications: I will be
monitoring you at these stations and giving you points upon
satisfactory completion (10-15 points)
when complete: students can either turn their paper in or
revisit stations one
at a time for a short period
Revisit the powerpoints for all of the labs
Review the lab manual pages
If we thought it was important enough to put in a powerpoint or
cover it in a quiz or do it as an activity, it’s important enough to
put on the exam!
Use the study guide found on Canvas

5. Biology Tutoring (Walker Science room 149)
Tutoring for BSC 110/111:
Monday 8am - 7pm
Tuesday 8am - 11am and 2:30pm - 5pm
Wednesday 8am - 5pm
Thursday 8am - 11am and 2:30pm - 3:30pm
Friday 9am - 10:30am
Tutoring for BSC 107/250/251:
Monday 10am – 12pm
Wednesday 10am – 12pm
Thursday 5:30pm – 7pm
Friday 8am – 12pm
A graduate student will be present who can help with lab reports
during the following times:
Tuesday 10am - 12pm and 2pm - 5pm
Wednesday 10am - 12pm and 2pm - 5pm

6. Thursday 10am - 12pm and 2pm - 5pm
Friday 10am - 12pm
From the syllabus: Students arriving more than 15 minutes late
for a lab practical will not be allowed to take the practical
during the allotted time. If a suitable excuse is provided to the
teaching assistant and approved by the lab coordinator, a make-
up lab practical may be scheduled.
In the event a student misses a lab practical due to family
emergency, illness, or University function, contact the Teaching
Assistant and Lab Coordinator immediately. If the student does
not contact the Teaching Assistant and Lab Coordinator within
48 hours of the exam, the student will not be allowed to make
up the missed assessment.
In addition, a verified excuse must be obtained from the
Assistant to the Vice President of Student Affairs in Union 219
and submitted to the Teaching Assistant and Lab Coordinator
within one week of the date of the exam. After review and
approval of the excuse, the Teaching Assistant and Lab
Coordinator will work with the student to arrange a date and
time for a make-up practical. A student is only allowed to make
up one lab practical during the semester. If there was no prior
contact and/or verified excuse obtained, as explained above, the
student will receive a score of zero on any assessment for that
day.
Today:

7. Activities: Enzymes Lab
Prepare for the week of the 7th:
Study!
Prepare for the week of the 21st:
Fill out Excel sheet with your data
Study for quiz on topics 4 and 5
Prelab for Topic 5: Visualizing Metabolism: Respiration and
Photosynthesis
Signature Assignment: Change Management
Purpose of Assessment
Develop specific strategies with supporting tactics to implement
positive change within an organization. You may refer to the
information that you prepared in Week 5 to complete this
assignment, but your submission should add to your prior
research and not just copy your Week 5 assignment.
Note: Do not copy information from a previous week. Use it as
a reference.
Scenario:
You’ve been hired as a consultant to develop strategies with
supporting tactics to implement positive changes for Hendrick
Health in Abilene, TX.
Review your analysis of the corporation’s change process from
Week 5 to be sure you have a thorough understanding of the
change and the need for the change.
Prepare a Change Management Essay for the Hendrick Health’s
Board of Directors. Include the following:
Outline of Essay
Paragraph 1
Introduction

8. Paragraph 2
Overview of Hendrick Health
Paragraph 3
What change needs to be made?
Paragraph 4, 5, and 6
Evaluate why this change needs to occur. Give three examples.
Each example has its own paragraph.
Paragraph 7
Discuss hos this change impacts Hendrick Health on a global
scale
Paragraph 8
Discuss how this change impacts employees
Paragraph 9
· Using Kotter’s 8-Step Change Model, Develop strategies for
each of the 8 steps in Kotter’s model chart strategies for
positively implementing the organizational change.
· Create chart and explain.
Paragraph 10
· Develop tactics to support each strategy. Chart tactics for
positively implementing the organizational change.
Create chart and explain.
Paragraph 11
· Justify the effectiveness of each strategy and corresponding
tactic with a rationale.
Paragraph 12
Conclude essay with an explanation of how this positive
organizational change will help the company sustain a
competitive advantage in the global market.
References
Format your paper in accordance with APA standards, include
in text citations (4 at a minimum), and use four references.