Seminario Biología Molecular, Tercer semestre Medicina Universidad Pontificia Bolivariana

1. Universidad Pontificia Bolivariana
Facultad de ciencias de la salud
Ana Sofía Córdoba
III semestre

2. INTRODUCTION
Symptoms:
- Cough: Dry or produce thick
- Difficulty breathing
- High temperature
- Loss of appetite
- Sweating and shivering
- Chest pain

3. INTRODUCTION
Multiplex PCR RT PCR
Multiplex RT PCR

4. GENERAL
OBJECTIVE
“Propose an accelerated
method for determining the
pathogen in a sample using
multiplex solid-phase RT-
PCR.”

5. MÉTODOS
Multiplex RT PCR Cepas
Multiplex RT-PCR es
capaz de la detección
simultánea de viroides
(ARN) y eubacterias (ADN)
Busca detectar
simultáneamente un
amplio espectro de
agentes patógenos
CEPAS DE BACTERIAS COLECCIONES DE CULTIVO
Streptococcus pneumoniae ATCC 25923
Staphylococcus aureus ATCC 43300
Haemophilus influenzae ATCC 49247
Legionella pneumophila ATCC 33152
Pseudomonas aeruginosa ATCC 25668 - 10662 NCTC
Klebsiella pneumoniae ATCC 13883 - 9633 NCTC
Influenza A -
SARS-Cov-2 -

6. MÉTODOS
Primers Electroforesis
Bromuro de
etidio
https://www.ibiantech.com/bromuro-de-etidio/
https://www.pngwing.com/es/free-png-dferl

7. RESULTADOS

8. RESULTADOS

9. DISCUSSION
AUTHOR AUTHOR´S POSTULATES AGREE OR DISAGREE WITH
THE ARTICLE
Inglis P.W., Pappas M.C.R.,
Resende L.V., Grattapaglia
Chiquito Almanza E., Acosta
Gallegos J.A., García Álvarez N.C.
They propose that is necessary
to unify the method for isolating
nucleic acids to create a
universal method for detecting
RNA and DNA pathogenic agents
known as CTAB method
Lung O., Fisher M., Beeston A.,
Hughes K.B., Clavijo A
Scuderi G., Catara A.F.,
Licciardello G
In most methods based on RT,
the reaction is carried out in the
liquid phase (without using
biochips), and only the
subsequent hybridization is
performed on the chip
Westin L., Xu X., Miller C., Wang
L., Edman C.F., Nerenberg M
Pemov A., Modi H., Chandler D.P.,
Bavykin S.
Approaches have been proposed
to carry out solid-phase
enzymatic reactions to create
compact, specialized biochips,
developed for isothermal
amplification, PCR bridge, and
next-generation sequencing
(NGS).

10. CONCLUSIONS
The Multiplex RT PCR manages to
identify among 8 of the most
common causes which is
responsible for presenting a clinical
picture of pneumonia in a patient to
start treatment promptly and
correctly.
Molecular biology is of the utmost
importance for medicine, since thanks
to discoveries and advances such as
Multiplex RT PCR, it is possible to make
an optimal and sooner diagnosis for the
patient

11. MAPA CONCEPTUAL

12. ¡THANK
YOU!