MS thesis presentation

1. Observational Record of Pre-mating and Mating
Behaviors of
Different Families of Butterflies in
Some Selected Areas of Bangladesh
Roll: 901
Reg. No.: HA-2571
Department of Zoology
University of Dhaka

2. INTRODUCTION
• Butterfly
• Pre-mating and mating behavior
Relation with abiotic factors
Different vegetation layers
Different time schedules

3. MATERIALS AND METHODS
• Study Period: March’15 to February’16
• Experimental procedures:
(Rutowski 1997; Lederhouse 1995)
• Emergent Sampling method
• Statistical analysis:
- Correlation-coefficient
- ANOVA
- Regression
Figure. Map of Bangladesh (red
circles are study areas)

4. RESULTS AND DISCUSSION
• Perching
behavior
• Patrolling
behavior
• Hair-pencil
behavior
• Pupal mating
Figure. Observed pre-mating and mating
occurrences
10
7
5
15
19
11
21
3 2
4
1
6
3
7
0
5
10
15
20
25
No.ofspecies
Butterfly families
Pre-mating
Mating

5. Figure. Fluctuation of observed pre-mating occurrences throughout
the study period (March’15 to February’16)
0
10
20
30
40
50
60
70
Absolutefrequency
(pre-mating)
Months
Lycaenidae
Papilionidae
Hesperiidae
Nymphalidae
Satyridae
Danaidae
Pieridae

6. Figure. Fluctuation of observed mating occurrences throughout the
study period (March’15 to February’16)
0
2
4
6
8
10
12
14
Absolutefrequency
(mating)
Months
Lycaenidae
Papilionidae
Hesperidae
Nymphalidae
Satyridae
Danaidae
Pieridae

7. Figure. Fluctuation of pre-mating and mating occurrences in relation to
different abiotic factors
0
20
40
60
80
45 50 55 60 65 70 75 80
Absolutefrequency
Humidity (%)
Pre-mating Mating
0
10
20
30
40
50
60
70
0 2 4 6 8
Absolutefrequency
Precipitation (mm)
Pre-mating Mating
0
20
40
60
80
10 10.5 11 11.5 12 12.5 13 13.5
Absolutefrequency
Day Length (hour)
Pre-mating Mating
0
20
40
60
80
18 20 22 24 26 28 30
Absolutefrequency
Temperature (°C)
Pre-mating Mating

8. • Statistical analyses (In case of observed pre-mating occurrences)
• Correlation coefficient values are,
Butterfly
Families
Humidity Precipitation Day length Temperature
Pieridae -0.44 -0.25 -0.07 0.05
Danaidae 0.05 0.37 0.40 0.43
Satyridae 0.10 -0.40 -0.07 0.29
Nymphalidae -0.80 -0.54 -0.29 -0.14
Hesperidae -0.37 -0.65 -0.55 -0.23
Papilionidae -0.12 -0.14 -0.02 0.47
Lycaenidae -0.44 -0.40 -0.11 0.10
F= 227.23 Fcrit= 1.928 P= 3.97E-63

9. • Statistical analyses (In case of observed mating occurrences)
• Correlation-coefficient values are
Butterfly
Families
Humidity Precipitation Day length Temperature
Pieridae -0.66 -0.77 -0.80 -0.33
Danaidae 0.76 0.79 0.94 0.98
Satyridae 0.43 0.22 0.20 0.73
Nymphalidae
Hesperidae -0.21 -0.61 -0.42 0
Papilionidae
Lycaenidae 0.14 -0.24 -0.25 0.05
F= 241.31 Fcrit= 1.99 P= 1.49E-44

10. Figure. Observed pre-mating and mating of butterflies in different
vegetation layers
0
20
40
60
80
100
120
Pieridae Danaidae Satyridae Nymphalidae Hesperiidae Papilionidae Lycaenidae
Pre-mating
frequency
Different butterfly families
Herb Shrub Tree
-2
0
2
4
6
8
10
12
14
Pieridae Danaidae Satyridae Nymphalidae Hesperidae Papilionidae Lycaenidae
Matingfrequency
Different butterfly families
Herb Shrub Tree

11. P= 0.0003
Figure. Observed pre-mating and mating occurrences of butterflies in
different time schedules
30
59
95
117
61
46
21
2
11
16 19
7 10
30
20
40
60
80
100
120
140
9.00-10.00 10.00-11.00 11.00-12.00 12.00-13.00 13.00-14.00 14.00-15.00 15.00-16.00
Absolutefrequency
Time schedule
Pre-mating Mating

12. CONCLUSION
- Monthly fluctuation observed
- Abiotic factors had significant impact
- Environmental biassness
- True interest in specific vegetation layer
- Peak interest towards specific time schedules of a day
ACKNOWLEDGEMENT
• Prof. Dr. M. A. Bashar
• Prof. Dr. Md. Anwarul Islam
• All teachers and stuffs
• EBBL researchers
• NST

13. Thank you.