This document lists 24 publications and presentations by Shaoxia Yu and collaborators from 2005 to the present related to bioanalysis and the development of methods for quantifying drugs and metabolites in biological samples using mass spectrometry. Key areas included using direct analysis in real time (DART) to enable bioanalysis without sample cleanup, microLC approaches for routine bioanalysis, and quantification of monoclonal antibodies and antibody-drug conjugates. Methods were applied to samples from multiple species for late stage drug discovery.
1. Page 1 of 4
SELECTED PUBLICATION
1. Shaoxia Yu, Jayaprakasam Bolleddula, Charles Locuson, Robert Griffin, Cindy Xia, Jing-Tao Wu,
Swapan Chowdhury "Prediction of Human Fg using Half-life Determined in Intestinal Microsomes”
In preparation.
2. Ling Xu, Bingli Ma, Shaoxia Yu, Cindy Xia and Jing-tao Wu “The Use of a Rapid Mass
Spectrometry Based Method for the Quantification of CYP 3A4 Protein Directly from Hepatocyte
Cell Lysate for CYP Induction Studies” Bioanalysis 2014, 6 (11).
3. Justin Gordon, Mike Johnson, Jing-Tao Wu, Shaoxia Yu* “A Simplified Approach for the
Simultaneous Qualification of Bioanalytical Methods for Multiple Species at Late Discovery Stage”
Bioanalysis 2012, 4(9).
4. Elizabeth Crawford, Justin Gordon, Jing-tao Wu, Brian Musselman, Ray Liu, Shaoxia Yu “Direct
Analysis in Real Time Coupled with Dried Spot Sampling for Bioanalysis in a Drug Discovery
Setting” Bioanalysis 2011, 3(11).
5. Matthew O. Duffey *, Ruth Adams, Christopher Blackburn, Ryan W. Chau, Susan Chen, Katherine
M. Galvin,Khristofer Garcia, Alexandra E. Gould, Paul D. Greenspan, Sean Harrison, Shih-Chung
Huang, Mi-Sook Kim,Bheemashankar Kulkarni, Steven Langston, Jane X. Liu, Li-Ting Ma, Saurabh
Menon, Masayuki Nagayoshi,R. Scott Rowland, Tricia J. Vos, Tianlin Xu, Johnny J. Yang, Shaoxia
Yu, “Discovery and optimization of pyrazoline compounds as B-Raf inhibitors” Bioorganic &
Medicinal Chemistry Letters 20 (2010) 4800–4804.
6. Shaoxia Yu ,Elizabeth Crawford, Joe Tice, Brian Musselman, Jing-Tao Wu “Bioanalysis without
Sample Cleanup or Chromatography: The Use of Direct Analysis in Real Time (DART) Ionization
Mass Spectrometry for the Direct Quantitation of Drugs in Biological Fluids” Analytical Chemistry,
2009, 81, 193–202.
7. Shaoxia Yu, Shelly Li, Hua Yang, Jing-tao Wu, Frank Lee, and Mark Qian “A Novel LC/MS/MS
Based Depletion Method for Measuring Red Blood Cell Partitioning of Pharmaceutical Compounds
in Drug Discovery” Rapid Communications in Mass Spectrometry, 2005; 19:250-254.
SELECTED PRESENTATIONS
1. Shaoxia Yu, Jayaprakasam Bolleddula, Charles Locuson, Robert Griffin, Cindy Xia, Jing-Tao Wu,
Swapan Chowdhury ‘Prediction of Human Fg using Half-life Determined in Intestinal Microsomes’
MDO 2016, Davis CA.
2. Ling Xu, Shaoxia Yu, Jing-Tao Wu, Mark Qian “Evaluation of Different Immunocapture Platforms
for Antibody-Conjugated Drug Quantification” The 64th
ASMS 2016 San Antonio, TX, USA.
3. Linlin Dong; Michael Johnson; Mark Qian; Shaoxia Yu “An Ion-pairing Strategy to Overcome PEG-
400 Caused Matrix Effect in Routine Drug Discovery Blood Sample Analysis” The 63th
ASMS 2015
St. Louis, MO.
4. Ling Xu, Shaoxia Yu, Jing-Tao Wu, Mark Qian “An LC/MS/MS Based Strategy for Quantification
of Monoclonal Antibodies and Antibody-conjugated Drugs for Developing Antibody Drug
Conjugates” CPSA 2015, Shang Hai, China.
5. Shaoxia Yu; Linlin Dong; Mike Johnson; Mark Qian; Chuang Lu “Development and Qualification of
a Method for Quantification of NAD in Biological Matrices” The 9th
WRIB, 2015, Miami, FL, USA
6. Susan Chen, Mark Qian, Shaoxia Yu “Chasing a “Ghost Peak”: Lessons Learned from a
Bioanalytical Method Qualification” APA, 2014 Boston.
7. Michael Johnson, Swapan Chowdhury, Patrick Kirby, Craig Fisher, Andy Lynn Jing-Tao Wu,
Shaoxia Yu “Development and Qualification of a Method for Quantitative Determination of Multiple
Bile Acids with microLC/MS/MS” the 62th
ASMS 2014 Baltimore, MN, USA.
8. Michael Johnson, Justin Gordon, Jing-Tao Wu, Shaoxia Yu “Evaluation of a micro liquid
chromatography approach for routine bioanalysis of small molecules” APA 2013 Boston.
2. Page 2 of 4
9. Justin Gordon, Ling Xu, Mike Johnson, Dmitry Blinder, Wendy Smith, Jing-Tao Wu, Shaoxia Yu
“An Investigation into the Lack of Protein Binding Equilibrium for Acidic Drug in Mouse Plasma”
APA 2013, Boston.
10. Benjamin Ngo, Shaoxia Yu, Ling Xu, Swapan Chowdhury, Jing-Tao Wu, Mike S. Lee, Gary
Valaskovic “The Design and Characterization of a Novel Ultra-low Flow Nanoelectrospray Device
for Metabolite Quantitation” The 61th ASMS 2013 Minneapolis, MN, USA.
11. Ling Xu, Shaoxia Yu, Jing-Tao Wu, “Novel High-throughput Bioanalysis for Basic Drug Candidates
Formulated in PEG”. Poster presentation at the 61th
ASMS Minneapolis, Minnesota,2013
12. Michael Johnson, Justin Gordon, Martin Paton, Jing-Tao Wu, Shaoxia Yu* “Elimination of carry-
over utilizing CTC HTS-xt in LC-MS analysis of biological samples” poster presentation at the 60th
ASMS 2012 Vancouver, Canda.
13. Justin Gordon, Mike Johnson, Jing-Tao Wu, Phil Geovanos, Shaoxia Yu* “Multi-Species
Simultaneous Qualification of Bioanalytical Methods for Compounds at Late Drug Discovery Stage”
poster presentation at the 60th
ASMS 2012 Vancouver, Canda.
14. Benjamin Ngo; Shaoxia Yu; Mike S. Lee; Jing-Tao Wu; Gary Valaskovic “Fundamental
Characteristics of Small Molecule Analysis using Ultra Low Dynamic Flow Nanospray1; poster
presentation at the 59th
ASMS 2011 Denver, CO .
15. Justin Gordon, Wu JT, Yu S. “One Stone Five Birds: A Simplified Approach for Non-GLP
Bioanalytical Method Qualification with Multiple Species. APA 2011, Boston.
16. Justin Gordon; Elizabeth Crawford; Jing-Tao Wu; Brian Musselman; Ming-xiang Liao; Bei-Ching
Chuang; Cindy Xia; David Ho; Lily Li; Shaoxia Yu* “Quantitative Analysis of Dried Blood Spots
by DART (Direct Analysis in Real Time) /MS/MS without Sample Preparation”, oral presentation
at 58th ASMS 2010 , Salt lake City, UT, USA.
17. Elizabeth Crawford, Shaoxia Yu, Lawrence Cohen, Justin Gordon, Brian Musselman, Jing-Tao Wu
“Eliminating Method Development, Sample Preparation and Chromatographic Separations in High-
Throughput Bioanalysis Using DART on an Enhanced Resolution Triple-Quadrupole Mass
Spectrometer”, oral presentation at 57th ASMS 2009, Philadelphia, PA.
18. Yu, S., Crawford, E., Tice, J., Musselman, B., and Wu, J-T., “Improving the Reproducibility and
Practicability of DART for the Direct Quantification of Drugs in Biological Fluids” oral
presentation at 56th ASMS 2008, Denvor, CO, USA.
19. Shaoxia Yu “Enabling Bioanalysis Without Sample Cleanup” oral presentation 2008 Vendor user
meeting, Denvor, CO.
20. Yu, S. X., Musselman, B., Tice, J., Crawford, E., and Wu, J-T.,” Bioanalysis without Sample
Cleanup or Chromatography: The Evaluation and Initial Implementation of DART Ionization Mass
Spectrometry for the Quantification of Drugs in Biological Matrixes” poster presentation at 55th
ASMS 2007 Indianapolis, IN, USA.
21. Shaoxia Yu, Shelly Li, Hua Yang, Jing-tao Wu, Frank Lee, and Mark Qian “A Fast LC/MS/MS
Screening Method for Evaluating Red Blood Cell Partitioning of Small Molecule Drugs” , poster
presentation at the 52th ASMS, 2004, Nashville, TN.
22. Shaoxia Yu, Jing-tao Wu, Mark Qian, “New Method Development for Rat Fat Tissue Sample
Preparation in Pharmacokinetics Study by LC-MS-MS”, poster presentation at The 20th
Montreux
LC/MS Symposium 2003, Savannah, GA, USA.
23. Lily Li, Ru Wei, Shaoxia Yu, Christa Beauregard, Arthur Rugg, Roger Wiegand, (Cereon); Sharon
Lu, Elizebth Baronas and Frank Hsieh (Millennium), “High Throughput Metabolic Profiling
Development Using Mass Spectrometric Techniques”; poster presentation at the 50th
ASMS , 2002.
Orlando, FL.
24. Shaoxia Yu, Arthur Rugg, and Lily Li (Cereon); Sharon Lu and Frank Hsieh (Millennium), “High
Throughput Analysis of Amino Acids in Biological Matrixes”, poster presentation at the 49th
ASMS
2001 Chicago IL.