Lipids are classified into different classes. simple lipids, compound lipids and derived lipids. In these slides one of the conpound lipid class glyco lipids are described. Ceramide: structure and functions are explained. Cerebrosides and gangliosides are also discussed. Diseases related to glyco lipids ae also enlisted.
2. Learning Objectives
By the end of this lecture students must be able
to:
❑ Sub-claasify Sphingo-phospholipids
❑Classify Glycolipids into subclasses
❑ Discuss the difference of Gangliosides and
cerebrosides in glycolipids
❑ Differentiate between different lipo-proteins
and their role in atherosclerosis
5. Sphingo-phospholipids
• A phospholipid with sphingosine instead of the
glycerol
• Important in the myelin sheath that surrounds most
nerve fibers, Grey matter –Sphingomyelin (found in
brain & nervous tissue).
• Found in special areas of membranes… known as
Lipid rafts.
6. Sphingo-phosopho lipids
• Sphingosine + fatty acid + PO4+ choline or ethanol
amine
• Ceramides.. Highly hydrophobic molecules
• Produced in response to stress stimuli (TNFs, radiation,
chemotherapeutic drugs)…Functions in different ways:
• Targets & activates enzymes (kinases, phosphatases)
• Regulates membrane structure
• Can act as a signaling molecule. The most well-known
functions of ceramides as cellular signals include
regulators of multiple cellular processes (apoptosis,
cell senescence, cell cycle, differentiation)
Ceramide
7. Sphingo-phosopho lipids
• Sphingomyelins
• 10-20% present in membranes.
• Brain, nerve tissues are rich in spingomyelins ..
Small quantities in blood & other tissues.
• Sphingomyelinase enzyme act on spingomyelins…
deficiency… accumulation of sphingomyelins in
spleen & brain. cause Niemann Pick’s disease
(mental retardation & enlargement of storage
tissues)
8. Phospholipids related Diseases
– Respiratory Distress syndrome.. Defic.
of lecithin
– Excess accumulation of sphingolipid
– Caused by an absent, mis made, or
deficient enzyme that breaks down the
lipid
– Multiple Sclerosis: Loss of myelin ----
sphingomyelin
– Slowing & eventual cessation of nerve
impulse
– Weakness, lack of co-ordination &
speech and vision problems
10. Glycolipids
• glycerol Sphingosine + 1 fatty acid + sugar
1- Cerebrosides 2- Gangliosides
– Present in outer leaflet of plasma membrane… cell
surface CHO
• The major glycolipids found in animal tissues are
glycosphingolipids.
– Function in cell adhesion & self-identity markers
– Important in the determination of an individual's
blood group
• They contain ceramide &one or more sugars.
e.g.Galactosylceramide
12. •A cerebroside is a sphingolipid (ceramide) with a
monosaccharide such as glucose or galactose as
polar head group. It contains a number of
characteristic C24 fatty acids e.g., cerebronic acid.
A ganglioside is a ceramide with a polar head
group that is a complex oligosaccharide, including
the acidic sugar derivative sialic acid.
Cerebrosides & gangliosides, collectively called
glycosphingolipids, are commonly found in the
outer leaflet of the plasma membrane bilayer, with
their sugar chains extending out from the cell
surface.
Glycolipids
13. Ceramide
• Highly hydrophobic molecules
• Produced in response to stress stimuli (TNFs,
radiation, chemotherapeutic drugs)
Functions in different ways:
• Targets and activates enzymes (kinases,
phosphatases)
• Regulates membrane structure
• Ceramide can actually act as a signaling molecule. The
most well-known functions of ceramides as cellular
signals include regulators of multiple cellular
processes (apoptosis, cell senescence, cell cycle,
differentiation)
15. Glycolipids
polar group
(containing sugars, more bulky)
Backbone (derived from serine in animals)
nonpolar fatty acid tail
(mainly saturated and longer tails
Gaucher’s disease-- enzyme gucocerbrosidase defi.
The fat builds up, especially in liver, spleen, and
bone marrow, causing problems.. mental
retardation & bone pain
G represents.. Ganglioside
M -------------… monosialo specie
1,2,3--------…Chromatographic migration
17. SULFOLIPIDS ( sulfatides)
• Lipids characterised by possessing sulphate groups
along with oligosaccharides
• Components of nervous tissue membranes… inborn
error is known as lipid storage diseases
18. Key Points
• Lipids when have phosphate group with sphingosine
alcohol…. Sphingo-Phospholipids… Sphingomyelins
• Glycolipids--- have one or more sugar units with alcohol
glycerol or sphingosine
Sulpho lipids…. Components of nervous tissue membrane
• Gaucher’s disease– Caused by deficiency of enzyme
gluco-cerebrosidase
Cerebrosides Gangliosides
19. Next topic of Discussion
• Lipoproteins, Cholesterol and Miscellaneous
lipids