Capitol Tech U Doctoral Presentation - April 2024.pptx
Late Stage adsorption of lipid vesicles
1. THE EARLY AND LATE PROCESSES IN THE
ADSORPTION OF PULMONARY
SURFACTANT
Samares C. Biswas1, Shankar B. Rananavare2, Stephen B. Hall1
1Oregon Health & Science University, Portland, OR, USA,
2Portland State University, Portland, OR, USA.
2. ADSORPTION OF PULMONARY SURFACTANT
• Pulmonary surfactant adsorbs to an air-water interface
in two distinct steps
– initial fall in surface tension at slowing rates to an
inflection point at ~35-50mN/m
– later acceleration until reaching the final surface
tension of ~25mN/m.
3. ADSORPTION OF LIPID VESICLES
Formation of lipid layer at the alveolar A/W interface
Multiple steps
•Diffusion
•Insertion
•Fusion
6. MODEL
• Step 1: Initial adsorption of lipid vesicle
– Does not require hydrophobic surfactant proteins (SP-B/-C)
– Related to a process other than fusion
• Step 2: Later acceleration of adsorption
– Requires the presence of proteins
– Fusion of the lipid vesicles with the preformed lipid layer
7. EFFECTS OF LPC & DSPE-PEG ON LIPID
STRUCTURES
• LPC
– forms micelles
– induces positive curvature
• which inhibit fusion
– produces lipid disorder
•DSPE-PEG
– forms micelles
– produces sterically stabilized liposomes
• which inhibit fusion
– produces lipid disorder
LPC-diagram
DOPE-PEG-
diagram
8. PREDICTED EFFECTS OF LPC AND DSPE-PEG
ON ADSORPTION
• LPC should
– inhibit the final step
– inhibition of fusion
– destabilization of stalk intermediate
• DSPE-PEG should
– inhibit the final step
– inhibition of fusion
– steric stabilization of liposome
15. LPC
Affected both the steps of adsorption.
Delayed the initiation of final acceleration.
Effect on the initial process is not clear.
16. LPC does have effect on the initial step, but it is
not clear. To determine the effect on the initial
step, we studied adsorption of LPC/DOPC and
LPC/N&PL systems.
22. LPC
Affected the initial fall in surface tension.
Effect was dose dependent.
Dialysis confirmed that the observed effect is
not due to the presence of free LPC.
27. Like LPC, DSPE-PEG also
Affected both the steps of adsorption
Delayed the initiation of final acceleration
Effects on initial process is not regular.
DSPE-PEG does accelerate the adsorption of
DOPC vesicles.
28. FINDINGS
Effects of LPC:
On CLSE and the model systems DOPE and GrA/DOPC-
Inhibit final steps of adsorption.
On N&PL and DOPC-
Accelerate the initial rate of adsorption.
Effects of DSPE-PEG:
CLSE and GrA/DOPC
Inhibit the initial and final steps of adsorption.
ON DOPC
Accelerate the initial rate of adsorption
29. CONCLUSIONS
Our results supports a model in which
Adsorption proceeds by two distinct steps:
Associated with two different structural effects.
Initial step
determined by different processes, such as
decreased bilayer order.
The final step
involves formation of a negatively curved
structure