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Reagents Manufacturer
TOTAL SOLUTION FOR
NUCLEIC ACID EXTRACTION
TANBead Blood DNA Kit is designed for rapid,
reliable, automated purifica�on of DNA from
blood samples. Our magne�c beads-based
technology with our corresponding extrac�on
system can provide you automated,
high-throughput and easy-to-use nucleic acids
extrac�on. The extracted nucleic acids can be
applied for various applica�ons, such as PCR,
qPCR, HLA-typing, and sequencing.
Introduc�on
Key features
TANBead® Blood DNA Kit
Blood DNA
Extrac�on
Automated magne�c beads-based
nucleic acids extrac�on technology
High yield and high-quality nucleic acids
Provide choices for different sample
inputs, such as 8, 48, 96 tests per run
Table 1.
Figure 1.
10 kb -
Whole blood
#1 #2 #3 #1 #2 #3 #1 #2 #3
Frozen blood Buffy coat
3 kb -
1 kb -
Integrity of DNA extracted from whole blood, frozen
blood and buffy coat using 61E kit, examined through
gel electrophoresis.
Yield and quality of extracted DNA from 300 μL whole
blood samples using 61E kit.
Yield (μg) 4.15
1.93 0.02
0.21
Quality
A260/A280
Mean SD
Specifica�on
Samples
Reagent kits
Extrac�on system
Applica�ons
Opera�on �me
PCR-based HLA-typing,
and NGS analysis
Whole blood, frozen blood,
buffy coat
50 - 60 min
61E series
Maelstrom 8 /
Maelstrom 48 series /
Maelstrom 96 series
Blood DNA Extrac�on
Table 1.
Figure 1.
Figure 2.
TANBead Blood RNA Kit is designed for rapid,
reliable, automated purifica�on of RNA from
blood samples. Our magne�c beads-based
technology with our corresponding extrac�on
system can provide you automated,
high-throughput and easy-to-use nucleic acids
extrac�on. The extracted nucleic acids can be
applied for various applica�ons, such as
real-�me PCR and RT-PCR.
Introduc�on
TANBead® Blood RNA Kit
Blood RNA
Extrac�on
Specifica�on
Samples
Reagent kits
Extrac�on system
Applica�ons
Opera�on �me
RT-PCR and RT-qPCR
Whole blood
45 - 55 min
621 series
Maelstrom 8 /
Maelstrom 48 series /
Maelstrom 96 series
Key features
Purify RNA from the whole blood sample
High yield and high-quality nucleic acids
Provide choices for different sample
inputs, such as 8, 48, 96 tests per run
Quan�fica�on Amplifica�on Plot
cycle
untreated
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39
treated
Rn
R2 = 0.9989
Log of
template
dilu�on
35
30
25
20
-4 -3 -2 -1 0
15
Cq
(B)
(A) The GAPDH products were successfully amplified
from the extracted RNA, along with its 10-fold serial
dilu�ons. (B) The linear rela�onship of Cq values of
each dilu�ons was demonstrated. The mean Cq values
of the samples were 20.22 ± 0.18, 23.52 ± 0.15, 26.00 ±
0.14, 29.11 ± 0.14, and 31.93 ± 0.43.
RNA in the extracted nucleic acid, observed by examin-
ing the GAPDH expression levels in the presence or
absence of DNase I treatment. The mean Cq value of
untreated group is 28.83 ± 0.67, and that of treated
group is 29.08 ± 0.45.
Yield and quality of extracted RNA from 100 μL whole
blood samples using 621 kit.
Yield (ng) 8.66
2.00 0.10
0.16
Quality
A260/A280
Mean SD
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39
Rn
Quan�fica�on Amplifica�on Plot
cycle
(A)
1x
d10x
d102x
d103x
d104x
Blood RNA Extrac�on
Table 1.
(B) Plasma
(A) Serum
TANBead cfDNA Kit is designed for rapid,
reliable, automated purifica�on of cfDNA from
plasma and serum samples. Our magne�c
beads-based technology with our correspond-
ing extrac�on system can provide you automat-
ed, high-throughput and easy-to-use nucleic
acids extrac�on. The extracted nucleic acids can
be applied to various applica�ons, such as PCR,
qPCR, and sequencing for oncology applica�on.
Introduc�on
cfDNA
Extrac�on
TANBead® cfDNA Kit
Specifica�on
Samples
Reagent kits
Extrac�on system
Applica�ons
Opera�on �me
PCR, qPCR and NGS anaylsis
Serum or plasma
55 - 70 min
61C series, L91C
Maelstrom 8 /
Maelstrom 24 series /
Maelstrom 48 series /
Maelstrom 96 series
Key features
Automated magne�c beads-based
nucleic acids extrac�on technology
High yield and high-quality nucleic acids
Provide choices for different sample
inputs, such as 8, 24, 48, 96 tests per run
Yield and integrity measurement of extracted cfDNA
from 4mL serum or plasma samples using L91C kit on
Maelstrom 24 series.
Figure 1.
The fragment size (red arrow) of extracted cfDNA from
the serum (A) or plasma (B) samples were examined
with capillary electrophoresis.
RFU
23.40
18.92
14.45
9.98
5.51
20 100 200
Length(bp)
300 400500600 1k 2k 3k
1.03
RFU
21.95
17.76
13.56
9.37
5.18
20 100 200
Length(bp)
300 400500600 1k 2k 3k
0.99
Sample
Serum
Plasma 20.3 1.43 0.49
0.2
2.56
88.9
Integrity
yield
(ng)
Alu115
(ng)
Alu247/
Alu115
cfDNA Extrac�on
Log IU/ml
TANBead
y=0.9862x-0.2143
R
2
=0.9637
8.00
7.00
6.00
5.00
4.00
3.00
2.00
1.00
A
1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00
Ct
ΔRn
10⁶ IU
10⁵ IU
10⁴ IU
10³ IU
10² IU
Threshold
(A) Swab Quan�fica�on Amplifica�on Plot
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 41 43
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39
Ct
ΔRn
10⁶ IU
10⁵ IU
10⁴ IU
10³ IU
10² IU
Threshold
(B) Sputum Quan�fica�on Amplifica�on Plot
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39
Ct
ΔRn
10⁶ IU
10⁵ IU
10⁴ IU
10³ IU
10² IU
Threshold
(C) BAL Quan�fica�on Amplifica�on Plot
The virus fragment was stably amplified in the extracted
RNA that isolated from samples containing various
concentra�on of HCV standard template. The sample
types, including swab (A), sputum (B) and BAL (C) were
examined.
Figure 1.
(A)
(B)
Figure 2.
1000
800
600
400
200
0
0 20
Amplfica�on
40
10 30
125 copies
E gene
Commercial-S
TANBead
RFU
500
400
300
200
100
0
0 20
Amplfica�on
40
10 30
125 copies
RFU
RdRP gene
Commercial-S
TANBead
1000
800
600
400
200
0
0 20
Amplfica�on
40
10 30
125 copies
RFU
N gene
Commercial-S
TANBead
1000
800
600
400
200
0
0 20
Amplfica�on
40
10 30
125 copies
RFU
IC
Commercial-S
TANBead
TANBead Virus DNA/RNA Kit is designed for
rapid, reliable, automated purifica�on of nucleic
acids from various sample types. Our magne�c
beads-based technology with our correspond-
ing extrac�on system can provide you automat-
ed, high-throughput and easy-to-use nucleic
acids extrac�on. The extracted nucleic acids can
be applied for various applica�ons, such as PCR,
qPCR, RT-PCR, RT-qPCR and sequencing.
Introduc�on
Key features
TANBead® Virus DNA/RNA Kit
Virus DNA/RNA
Extrac�on
Automated magne�c beads-based
nucleic acids extrac�on technology
High yield and high-quality nucleic acids
Provide choices for different sample
inputs, such as 8, 48, 96 tests per run
Specifica�on
Samples
Reagent kits
Extrac�on system
Applica�ons
Opera�on �me
PCR, qPCR and sequencing
Serum, plasma, swabs,
sputum, or bronchoalveolar
lavage (BAL)
35 - 45 min
665 series
Maelstrom 8 /
Maelstrom 48 series /
Maelstrom 96 series
(A) In sixty HCV posi�ve samples, high correla�on
between TANBead viral extrac�on kit and commercial-A
all-in-one sample prepara�on and detec�on system was
demonstrated (X-axis: Log IU/ml of HCV RNA extracted
by the 665 kit Y-axis: Log IU/ml of HCV RNA extracted by
commercial-A sample prepara�on system) (B) samples
spiked with 125 copies of
COVID-19 pseudovirus were
extracted using 665 or com-
mercial S kit and were subject-
ed to qPCR analysis for N, E,
RdRP, and internal control (IC).
Virus DNA/RNA Extrac�on
TANBead Virapid Viral Extrac�on Kit is designed
for those who were struggling to isolate nucleic
acids from viral samples. Our technology
provides the solu�on to complete the whole
extrac�on processes in about 15 minutes. The
extracted nucleic acids can be applied to
applica�ons, such as PCR, qPCR and RT-PCR.
Introduc�on
Key features
TANBead® Virapid Virus Kits
Virapid Virus
Extrac�on
Simply transfer the sample to the
pre-filled plate/tube for extrac�on
without addi�onal proteinase K
Ultra-fast process which takes only about
15 minutes
Provide choices for different sample
inputs, such as 8, 48, 96 tests per run
Specifica�on
Samples
Reagent kits
Extrac�on system
Applica�ons
Opera�on �me
PCR, qPCR, RT-PCR
Nasal, nasopharyngeal, or
oropharyngeal swab,
saliva, urine
14 - 17 min
685 series
Maelstrom 8 /
Maelstrom 48 series /
Maelstrom 96 series
The automa�c extrac�on opera�on �me of 685 series
kits were shown as below: 16.33 min (M9600), 14.23
min (M4800), 14.31 min (M8).
Figure 1.
Figure 2.
The extrac�on performance of the M685A46 kit was
examined by three qPCR kits, (A) Vircell, (B) Seegene,
(C) SolGent, and the results indicated that M685A46
kit's extrac�on performance is comparable to M665A46
kit. COVID-19 pseudovirus (500 copies) spiked in virus
transporta�on medium (VTM) were used as the
extrac�on samples.
(A) Vircell
Direct SARS-CoV-2 qPCR kit
N gene E gene RNaseP
Target gene
Ct
value
30
35
40
25
20
665 series
685 series
665 series
685 series
N gene E gene RdRP
(B) Seegene
AllPlex 2019-nCoV Assay
Target gene
Ct
value
30
35
40
25
20
Minute
Opera�on Time
M8
M4800
M9600
0 20 40
665 series
685 series
Virapid Virus Extrac�on
Figure 3.
Saliva and urine samples were spiked with 500 copies of COVID-19 pseudovirus. Samples were extracted with M685A46
kit and results showed that the kit can extract viral nucleic acid from saliva and urine.
(C) SolGent
DialPlexQ 2019-nCoV Detec�on kit
665 series
685 series
N gene ORF1a PCRC
Target gene
Ct
value
30
35
40
25
20
Saliva Urine
Vircell
Direct SARS-CoV-2 qPCR kit
30
35
40
25
20
N gene
E gene
RNaseP
Figure 4.
Eight clinical samples were subjected to nucleic acids extrac�on using 685 and 665 kits, and the extracts was analyzed
with Vircell - DIRECT SARS-COV-2 REALTIME PCR KIT. Results showed high correla�on between 685 and 665 kit's Ct values
of extracted N,E and RNaseP genes.
The detec�on performance correla�on of clinical
sample by 685 and 665 kits
20
20
25
25
30
30
35
35
685 Ct Value
665
Ct
Value
40
40
45
45
N gene
E gene
RNaseP
N gene E gene RNaseP
0.982 0.985 0.978
Ct value
correla�on
between 2 kits
Virapid Virus Extrac�on
The TANBead HPV DNA Nucleic Acid Extrac�on
Kit is designed to perform the HPV nucleic acids
extrac�on. By using TANBead Nucleic Acid
Extrac�on Systems, the one-step-to-extrac�on
can be performed automa�cally. Cervical swabs
or liquid based cytology samples are processed
through a series of automa�c extrac�on steps
and the high-quality nucleic acids can be
applied directly for further applica�ons. The
extracted nucleic acids can be applied for
qualita�ve and quan�ta�ve molecular analyses,
such as real-�me PCR.
Introduc�on
HPV DNA
Extrac�on
TANBead® HPV DNA Kit
Specifica�on
Samples
Reagent kits
Extrac�on system
Applica�ons
Opera�on �me
PCR and qPCR
cervical swab,
liquid based cytology samples
35 - 45 min
61H series
Maelstrom 8 /
Maelstrom 48 series
Maelstrom 96 series
Key features
Automated magne�c beads-based
nucleic acids extrac�on technology and
prefilled reagent system
Mucus specimens are applicable through
a simple pretreatment step
Provide choices for different sample
inputs, such as 8, 48, 96 tests per run
Virus fragments were stably extracted and amplified
from samples containing various concentra�ons of
cervix cells with human papillomavirus type 16
genome. The sample types including (a) cervical swab
(b) liquid based cytology, were examined.
Figure 1.
The 61H kit can stably perform nucleic acids extrac�on
from both mucus and non-mucus specimens. The SiHa
cells containing HPV 16 genome, or the RWPE-1 cells
containing HPV 18 genome were subjected to nucleic
acids extrac�on and qPCR analysis. The Ct value results
of mucus samples were similar to those of non-mucus
samples.
Figure 2.
Non-mucus
Pre-treatment
Non-mucus Mucus
Mucus
HPV 18
HPV 16
Ct
value
28
+ - + -
30
32
34
RFU
0
0 10 20 30 40 50
500
1000
1500
2000
2500
3000
(b) liquid based cytology Amplifica�on
105
104
103
102
Cycles Threshold
RFU
0
0 10 20 30 40 50
500
1000
1500
2000
2500
3000
(a) cervical swab Amplifica�on
105
104
103
102
Cycles Threshold
HPV DNA Extrac�on
Table 1.
Clinical inves�ga�on result of HPV-posi�ve or nega�ve clinical specimens (stored in ThinPrep) for HPV type 16,18 or 45
detec�ons, using 61H kits for nucleic acids extrac�on. These specimens were purchased and have been validated for HPV
types using Hologic HPV detec�on kit.
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
33.08
25.64
23.49
25.08
29.19
25.13
27.82
32.37
26.31
23.29
28.46
31.42
23.15
30.29
29.35
34.28
30.96
32.55
28.22
27.95
41.3
30.64
33.8
37.56
25.3
25.15
20.29
30.98
S00880598
S00880599
S00880600
S00880601
S00880602
S00880603
S00880604
S00880605
S00880606
S00880607
S00880608
S00880609
S00880610
S00880611
S00880612
S00880613
S00880614
S00880615
S00880616
S00880617
S00880648
S00880649
S00880650
S00880652
S00880653
S00880654
S00880655
S00880656
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
HPV 16/18/45 (-)
S000880625
S000880626
S000880627
S000880630
S000880631
S000880632
S000880635
S000880636
S000880637
S000880640
S000880641
S000880642
S000880645
S000880647
S000880618
S000880619
S000880620
S000880621
S000880622
S000880623
S000880624
S000880628
S000880629
S000880633
S000880634
S000880638
S000880639
S000880643
HPV 16 (+)
HPV 16 (+)
HPV 16 (+)
HPV 16 (+)
HPV 16 (+)
HPV 16 (+)
HPV 16 (+)
HPV 16 (+)
HPV 16 (+)
HPV 16 (+)
HPV 16 (+)
HPV 16 (+)
HPV 16 (+)
HPV 16 (+)
HPV 45 (+)
HPV 18 (+)
HPV 18 (+)
HPV 18 (+)
HPV 18 (+)
HPV 18 (+)
HPV 45 (+)
HPV 18 (+)
HPV 45 (+)
HPV 45 (+)
HPV 45 (+)
HPV 18 (+)
HPV 18 (+)
HPV 45 (+)
Nega�ve sample Remarks Remarks
Posi�ve sample
Ct value Ct value
N.O.
HPV DNA Extrac�on
Table 1.
Figure 1.
The yield and quality of extracted DNA from 106 Salmo-
nella or Staphylococcus using the 61G kit.
Genomic DNA from 14 gram-posi�ve and gram-nega-
�ve bacteria is well isolated using the 61G kit.
TANBead Bacteria DNA Kit is designed for rapid,
reliable, automated purifica�on of nucleic acids
from gram(-), gram(+) and other atypical
bacteria samples. Our magne�c beads-based
technology with our corresponding extrac�on
system can provide you automated,
high-throughput and easy-to-use nucleic acids
extrac�on. The extracted nucleic acids can be
applied for various applica�ons, such as PCR,
qPCR, and sequencing.
Introduc�on
Key features
TANBead® Bacteria DNA Kit
Bacteria DNA
Extrac�on
Automated magne�c beads-based
nucleic acids extrac�on technology
High yield and high-quality nucleic acids
Provide choices for different sample
inputs, such as 8, 48, 96 tests per run
Specifica�on
Samples
Reagent kits
Extrac�on system
Applica�ons
Opera�on �me
PCR, qPCR, and sequencing
Sputum, bronchoalveolar
lavage (BAL), or cultured
bacteria
61G (70 - 80 min)
61G-SE (45 - 55 min)
61G series, 61G-SE series
Maelstrom 8 /
Maelstrom 48 series /
Maelstrom 96 series
1: Bacillus
2: Microbacterium
3: Massilia
4: Paenibacillus
5: Corynebacterium
6: Escherichia
7: Sphingomonas
8: Cupriavidus
9: Duganella
10: Flavobacterium
11: Lactobacillus
12: Weissella
13: Leuconostoc
14: Burkholderia
1 2 3 4 5 6 7 8 9 10 11 12 13 14
Salmonella Staphylococcus
Mean Mean
SD
33.1 0.8 0.21
0.04
2.04
0.02
2.06
34.3
SD
Yield (μg)
Quality
A260/A280
- gDNA
Bacteria DNA Extrac�on
Figure 2.
Five cerebrospinal fluid (CSF) clinical samples with unknown pathogen infec�on were subjected to nucleic acids extrac�on
and the extracts were analyzed through qPCR analysis to detect N. meningitidis (bacteria) and C. neoformans (fungi). The
results demonstrated that 61G kit is applicable for nucleic acids extrac�on from CSF samples.
Figure 3.
Eight liquid culture samples of M. tuberculosis were subjected to nucleic acids extrac�on and the extracts were analyzed
with qPCR analysis. The results demonstrated that 61G kit is applicable for nucleic acids extrac�on of TB liquid culture
samples.
N. meningitidis
C. neoformans
Target
qPCR results (shown as Ct value)
wl-35663
wl-38969
CSF clinical
sample #01
CSF clinical
sample #02
CSF clinical
sample #03
CSF clinical
sample #04
CSF clinical
sample #05
CN4
CN5
36.43
ND
45.13
ND
ND
ND
38.27
ND
33.86
37.62
Home-made
Primer set
Liquid
culture
#01
19.7
Liquid
culture
#02
20.67
Liquid
culture
#03
21.28
Liquid
culture
#04
20.78
Liquid
culture
#05
23.06
Liquid
culture
#06
17.35
Liquid
culture
#07
21.14
Liquid
culture
#08
18.81
Posi�ve
control
(300
copies)
21.91
NTC
ND
qPCR results (shown as Ct value)
Target
M. tuberculosis
Bacteria DNA Extrac�on
Yield and quality of extracted DNA from meat, zebraf-
ish, or cells using 6T2 kit.
Confirma�on of DNA integrity extracted from meat,
zebrafish or leukocyte using 6T2 kit, examined through
gel electrophoresis.
Table 1.
Figure 1.
TANBead Tissue DNA Kit is designed for rapid,
reliable, automated purifica�on of DNA from
�ssues and cells. Our magne�c beads-based
technology with our corresponding extrac�on
system can provide you automated,
high-throughput and easy-to-use nucleic acids
extrac�on. The extracted nucleic acids can be
applied for various applica�ons, such as PCR,
qPCR, and sequencing.
Introduc�on
Tissue DNA
Extrac�on
TANBead® Tissue DNA Kit
Specifica�on
Samples
Reagent kits
Extrac�on system
Applica�ons
Opera�on �me
PCR, qPCR and Southern blot
Tissues or cells
70 - 80 min
6T2 series
Maelstrom 8 /
Maelstrom 48 series /
Maelstrom 96 series
Key features
Automated magne�c beads-based
nucleic acids extrac�on technology
High yield and high-quality nucleic acids
Provide choices for different sample
inputs, such as 8, 48, 96 tests per run
10 kb -
3 kb -
1 kb -
Meat
#1 #2 #1 #2 #1 #2
Zebrafish Leukocyte
31.09
Yield (μg)
Quality
A260/A280
0.61 29.29 0.54 22.52 0.01
Mean SD Mean SD Mean SD
1.81 0.02 1.8 0 1.98 0.51
50 mg
meat
50 mg
zebrafish
106
cells
Tissue DNA Extrac�on
Figure 1.
Table 1.
Integrity of RNA from 30 mg zebrafish, 30 mg meat and
106 THP-1 or SHSY5Y cells extracted with 6K2 kit, exam-
ined through gel electrophoresis.
Yield and quality of extracted RNA from zebrafish, meat
and cells (THP-1 or SHSY5Y) using 6K2 kit.
TANBead Tissue RNA Kit is designed for rapid,
reliable, automated purifica�on of RNA from
�ssues and cells. Our magne�c beads-based
technology with our corresponding extrac�on
system can provide you automated,
high-throughput and easy-to-use nucleic acids
extrac�on.The extracted nucleic acids can be
applied for various applica�on, such as RT-PCR.
Introduc�on
Tissue RNA
Extrac�on
TANBead® Tissue RNA Kit
Specifica�on
Samples
Reagent kits
Extrac�on system
Applica�ons
Opera�on �me
RT-PCR,qRT-PCR and
Northern blot
Tissues or cells
45 - 60 min
6K2 series
Maelstrom 8 /
Maelstrom 48 series /
Maelstrom 96 series
Key features
Automated magne�c beads-based
nucleic acids extrac�on technology
High yield and high-quality nucleic acids
Provide choices for different sample
inputs, such as 8, 48, 96 tests per run
Yield (μg)
Quality
A260/A280
Zebrafish Meat THP-1 SHSY5Y
15.21±1.12 12.12±0.65 18.34±0.81 26.29±1.05
1.94±0.01 1.95±0.03 1.98±0.01 1.99±0.02
Meat
#1 #2 #1 #2
Zebrafish
3 kb -
1 kb -
0.2 kb -
THP-1
#1 #2 #3 #3 #4
#1
#4 #2
SHSY5Y
2 kb -
1 kb -
0.2 kb -
Tissue RNA Extrac�on
TANBead FFPE DNA Kit is designed for rapid,
reliable, automated purifica�on of DNA from
FFPE samples. Our magne�c beads-based
technology with our corresponding extrac�on
system can provide you automated,
high-throughput and easy-to-use nucleic acids
extrac�on. The extracted nucleic acids can be
applied for various applica�on, such as PCR,
qPCR, and sequencing.
Introduc�on
FFPE DNA
Extrac�on
TANBead® FFPE DNA Kit
Specifica�on
Samples
Reagent kits
Extrac�on system
Applica�ons
Opera�on �me
PCR, qPCR, and sequencing
FFPE
20 - 30 min
61P series
Maelstrom 8 /
Maelstrom 48 series /
Maelstrom 96 series
Key features
Only 5 μm thick FFPE sample is enough
for use
Saving pretreatment �me
No toxic solvents throughout the whole
extrac�on process
Figure 1.
The qPCR (A) amplifica�on curves and (B) Ct values of
FFPE DNA extracted with TANBead and commercial T
kits.
Table 1.
The comparison of nucleic acids extrac�on perfor-
mance between TANBead FFPE kits and Commercial-T
FFPE kits in extrac�ng 20 mg pig liver FFPE samples.
11.01±0.52
7.70±0.06
137.65±6.55
91.67±0.76
TANBead
Commercial-T
1.91±0.01
2.00±0.01
Yield (μg) A260/280
Extrac�on
System
Conc. (ng/μL)
TANBead
Commercial-T
28.78±0.18
31.06±0.88
Mean Ct
Extrac�on
System
(B)
(A)
RFU
0
-100
0 10 20 30 40
100
200
300
400
500
600
Amplifica�on
Cycles
Blue: TANBead
Red: Commercial-T
FFPE DNA Extrac�on
Figure 1.
Yield comparison of extracted stool DNA using 6SC kit
and commercial-Q kit.
The extracted stool DNA was subjected to 16S rDNA
(indicates bacteria) or GAPDH (indicates human) qPCR
analysis, and the Ct values of samples extracted with
6SC kit were lower than that extracted with commer-
cial-Q kit.
TANBead Stool DNA Kit is designed for rapid,
reliable, automated purifica�on of DNA from
stool samples. Our magne�c beads-based
technology with our corresponding extrac�on
system can provide you automated,
high-throughput and easy-to-use nucleic acids
extrac�on. The extracted nucleic acids can be
applied for various applica�ons, such as PCR,
qPCR, sequencing (microbiome profiling).
Introduc�on
Stool DNA
Extrac�on
TANBead® Stool DNA Kit
Specifica�on
Samples
Reagent kits
Extrac�on system
Applica�ons
Opera�on �me
PCR, qPCR, and NGS analysis
Stool
40 - 55 min
6SC series
Maelstrom 8 /
Maelstrom 48 series /
Maelstrom 96 series
Key features
Can acquire both the microbial and the
host DNA from stool samples
Provides appropriate lysis buffers for
either omnivorous or herbivorous
species
Provide choices for different sample
inputs, such as 8, 48, 96 tests per run
Q
Supplier TANBead
SD Mean
Mean SD
130.1
Yield (ng/µL) 2.4 11.9 0.4
Cycle
threshold
TANBead Q
50
20
30
40
10
0
GAPDH
Cycle
threshold
16S
TANBead Q
25
10
15
20
5
0
Table 1.
Stool DNA Extrac�on
Table 3.
Yield measurement and Ct values of extracted DNA from stool samples spiked with Giardia lamblia cyst parasites.
Table 2.
Yield measurement and 16s qPCR analysis of extracted DNA from stool samples of omnivorous or herbivorous species.
TANBead
Species
Cat
Dog
Human
Yield (μg)
14.71
40.42
32.68
SD
0.06
0.04
0.1
Ct
31.7
32.35
29.53
SD
0.23
0.25
0.17
Cat
Dog
Rabbit
Chinchilla
Goat
Tortoise
Guinea Pig
Cow
14.53±1
26.58±0.67
6.4±0.22
18.35±3.8
3.5±1.25
10.15±1.59
37.5±7.60
4.53±0.3
27.37±0.62
17.14±0.36
NA
NA
20.22±0.66
28.14±0.71
27.63±0.74
29.99±0.43
5.22±0.51
2.38±0.39
3.25±0.6
4.08±0.46
5.03±1.42
5.2±2.02
33.8±15.64
7.3±1.1
29.32±0.32
Yield (μg) Ct Mean Yield (μg) Ct Mean
19.56±0.28
28.07±0.21
28.65±0.18
20.1±0.51
28.16±0.6
27.62±1.16
29.48±0.46
Species
Incuba�on buffer 1:
Omnivorous Buffer
Incuba�on buffer 2:
Herbivorous Buffer
Stool DNA Extrac�on
Table 1.
Yield and quality of extracted DNA from plant samples
using 613 kit.
Figure 1.
Integrity of DNA extracted from plant samples using 613
kit, examined through gel electrophoresis.
M
Leaves
#1 #2 #1 #2 #1 #2 #1 #2 #1 #2 #1 #2 #1 #2 #1 #2
Seeds
R
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e
S
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y
A
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a
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TANBead Plant DNA Kit is designed for rapid,
reliable, automated purifica�on of DNA from
plant materials. Our magne�c beads-based
technology with our corresponding extrac�on
system can provide you automated,
high-throughput and easy-to-use nucleic acids
extrac�on. The extracted nucleic acids can be
applied for various applica�ons, such as PCR,
qPCR, and sequencing.
Introduc�on
Plant DNA
Extrac�on
TANBead® Plant DNA Kit
Specifica�on
Samples
Reagent kits
Extrac�on system
Applica�ons
Opera�on �me
PCR-based genotyping assay
and qPCR
Leaf , seed or rice grain
613 (45 - 55 min)
613-SE (50 - 60 min)
619 (100 - 120 min)
613 series, 613-SE series,
619 series
Maelstrom 8 /
Maelstrom 48 series /
Maelstrom 96 series
Key features
Automated magne�c beads-based
nucleic acids extrac�on technology
High yield and high-quality nucleic acids
Provide choices for different sample
inputs, such as 8, 48, 96 tests per run 10 kb -
3 kb -
1 kb -
Figure 2.
Yield, quality and integrity of extracted DNA from rice
grain samples using 619 kit.
10 kb -
3 kb -
1 kb -
#1 #2
One grain
Mean SD
Yield (μg)
Quality
A260/A280
14.1
2.33
3.24
0.33
Sample type
Leaves
Rice 4.93±0.13
4.79±0.53
3.41±0.04
5.84±0.62
2.5±0.03
2.11±0.22
4.19±0.03
15.05±0.24
1.82±0.02
1.37±0.05
1.89±0.06
1.67±0.11
1.98±0.01
1.88±0.04
1.84±0.01
1.82±0.02
Strawberry
Arabidopsis
Eucalyptus
Laurel
Wheat
Tomato
Co�on
Seeds
Yield
(μg)
Quality
260/280
Plant DNA Extrac�on
Various leaves DNA are well extracted using the M613-SE
kit.
Various seeds DNA are well extracted using the M613-SE
kit.
Table 2. Table 3.
Fern
Cunninghamia lanceolata
Juniper us chinensis L. var. kaizuka
Pinaceae
Podocarpus macrophyllus
Commelina communis L.
Bambusoideae
Egeria densa
Orchidaceae, Orchid
Saccharum
Areca catechu
Oryza sativa, Rice
Trachycarpus fortunei, Palm
Scheffera arboricola
Melon
Cabbage
Trifolium hybridum
Phoebe zhennan
Prunus subgen. Cerasus
Psidium guajava
Aronia melanocarpa
Fructus Mori
Corymbia citriodora
Melaleuca alternifolia
Eucalyptus robusta
Camellia sinensis
Liquidambar formosana
Osmanthus fragrans
Codiaeum variegatum
Acacia confusa
Carica papaya
Rosa rugosa, Rose leaf
Rosa rugosa, Rose petal
Passiflora edulis
Celosia cristata
Corymbia citriodora
Laurus nobilis
Arabidopsis thaliana
Fragaria × ananassa, Strawberry
16.1
9.4
25.2
6.2
11.9
27.1
36.8
19.6
22.3
43.6
27.25
32.03
27.25
17
22.9
3.4
16.7
13.8
28.3
28.4
30
18.4
27.4
36.5
41.5
47.1
12.6
12.5
53.6
41.7
26.4
35.2
8.3
26.3
12.7
18.7
14.3
24.77
37.29
Plant leaves Conc. (ng/µL)
Zea mays, Corn
Hordeum vulgare, Barley
Triticum aestivum
Arabidopsis thaliana
Sesamum indicum
Cucumis sativus, Cucumber
Cucurbita pepo, Pumpkin
Abelmoschus esculentus
Fragaria × ananassa, Strawberry
Solanum lycopersicum, Tomato
Solanum melongena, Egg Plant
Co�on
Alstonia scholaris
10.0
10.2
17.4
51.1
8.6
16.0
10.8
14.1
13.3
32.4
17.0
117.1
9.4
Conc. (ng/µL)
Plant seeds
Plant DNA Extrac�on
TANBead Plant RNA Kit is designed for rapid,
reliable, automated purifica�on of RNA from
the leaves or seeds. Our magne�c beads-based
technology with our corresponding extrac�on
system can provide you automated,
high-throughput and easy-to-use nucleic acids
extrac�on. The extracted nucleic acids can be
applied for various applica�on, such as RT-PCR.
Introduc�on
Plant RNA
Extrac�on
TANBead® Plant RNA Kit
Specifica�on
Samples
Reagent kits
Extrac�on system
Applica�ons
Opera�on �me
RT-PCR, qRT-PCR and
Northern blot
Leaf or seed
45 - 60 min
6K3 series
Maelstrom 8 /
Maelstrom 48 series /
Maelstrom 96 series
Key features
Automated magne�c beads-based
nucleic acids extrac�on technology
High yield and high-quality nucleic acids
Provide choices for different sample
inputs, such as 8, 48, 96 tests per run
Table 1.
Yield and quality of extracted RNA from plant samples
using 6K3 kit.
Figure 1.
Integrity of RNA extracted from leaves or seeds using
6K3 kit, examined through gel electrophoresis.
M
Leaves Seeds
R
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e
S
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a
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A
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a
b
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#1 #2 #1 #2 #1 #2 #1 #2 #1 #2 #1 #2 #1 #2 #1 #2
1.5 kb -
0.5 kb -
0.1 kb -
6.46±0.16
6.46±0.18
6.12±0.24
6.17±0.10
6.18±0.22
6.68±0.20
4.45±0.15
4.9±0.04
Rice
Strawberry
Arabidopsis
Eucalyptus
Laurel
Wheat
Tomato
Co�on
1.97±0.01
1.97±0.01
1.95±0
1.94±0.04
1.96±0.01
1.95±0.04
1.72±0.06
2.06±0.18
Yield
(μg)
260/280
Sample type
Leaves
Seeds
Plant RNA Extrac�on
TANBead Fungi DNA Kit is designed for rapid,
reliable, automated purifica�on of DNA from
fungi culture. Our magne�c beads-based
technology with our corresponding extrac�on
system can provide you automated,
high-throughput and easy-to-use nucleic acids
extrac�on. The extracted nucleic acids can be
applied for various applica�ons, such as PCR,
qPCR, and sequencing.
Introduc�on
Fungi DNA
Extrac�on
TANBead® Fungi DNA Kit
Specifica�on
Samples
Reagent kits
Extrac�on system
Applica�ons
Opera�on �me
PCR and qPCR
Fungi
50 - 60 min
61F series
Maelstrom 8 /
Maelstrom 48 series
Key features
Automated magne�c beads-based
nucleic acids extrac�on technology
High yield and high-quality nucleic acids
Provide choices for different sample
inputs, such as 8, 48 tests per run
Table 1.
Yield and quality of extracted DNA from yeast using 61F
kit.
1 OD 2 OD
Mean
0.81
1.95
SD
0.02
0.39
0.021
Mean
Yield (μg)
1.96
Quality
A260/A280
SD
0.035
0.01
Figure 1.
Integrity of DNA extracted from yeast samples using
61F kit, examined through gel electrophoresis.
1 OD 2 OD
#1 #2 #3 #1 #2 #3
3 kb -
10 kb -
1 kb -
M
Figure 2.
PCR amplifica�on of the tandemly repe��ve subele-
ments (TRS)-1 and TRS-2 from five isolates of T.
rubrum.(Chien-yio Lin, 2018)
Reference.
Chien-yio Lin, Hsiu-Jung Lo, Ming-Gene Tu et al. The
survey of �nea capi�s and scalp dermatophyte carriage
in nursing home residents. Medical Mycology. 2018;
56:180-185.
M 1 2 3 4 5 M M 1 2 3 4 5 M
800 -
500 -
(bp)
- 800
- 500
(bp)
TRS-1 TRS-2
1: scalp 2: scalp 3: scalp
4: right sole 5: right big toe
Fungi DNA Extrac�on
Food and Feed DNA
Extrac�on
TANBead® Nucleic Acid Extrac�on Kit (6GM) is
designed for a simple and convenient method of
high-quality DNA isolated from raw material and
recover the highly fragmented DNA from
processed food, which can be down to 100 bp.
With TANBead® automated extractors, this kit
can simplify the nucleic acid extrac�on process,
abstaining from manual processing. The �me
needed is reduced, repe��ve centrifuga�on is
removed and risk of cross-contamina�on is
minimized. The extracted DNA is ready-to-use for
detec�on of gene�cally modified organisms
(GMO) and food adultera�on.
Introduc�on
TANBead® Food and Feed DNA Kit
Specifica�on
Samples
Reagent kits
Extrac�on system
Applica�ons
Opera�on �me
PCR-based assays
Food material or products,
Feed
45 - 55 min
6GM series
Maelstrom 8 /
Maelstrom 48 series
Maelstrom 96 series
Key features
Automated magne�c beads-based
nucleic acids extrac�on technology and
prefilled reagent system
Can recover short DNA fragments (down
to 100 bp) in processed food
Provide choices for different sample
inputs, such as 8, 48, 96 tests per run
Figure 1.
The performance of Food and Feed DNA Auto Kit was
examined through qPCR analysis. The results indicated
that the extracted DNA can be used for GMO detec�on
(using Thermo GMO Screening kit). GMO (50 mg) was
extracted, then the P35 and TNOS genes were detected
with qPCR along with PC (posi�ve control), IC (internal
control) and NC (nega�ve control).
Table 1.
The DNA extracted from 50 mg sample was examined
with Qubit™ dsDNA HS assay kit.
Corn
Soybean
Chip
Cereal
Tofu
Miso
Dog food
Pig food
20-40 ng / µL
25-50 ng / µL
2-10 ng / µL
0-10 ng / µL
10-30 ng / µL
5-20 ng / µL
10-35 ng / µL
10-50 ng / µL
Expected yield
Product type
Sample : GMO soy
Target : P35S, TNOS
P35S (Sample)
P35S (PC)
TNOS (PC)
TNOS (Sample)
NC
Amplifica�on
Cycles
RFU
10 30 50
0 20 40
0
2000
4000
1000
3000
Sample : GMO soy
Target : IC (plant)
Amplifica�on
Cycles
RFU
10 30 50
0
0
1000
2000
3000
500
1500
2500
20 40
NC
IC
Food and Feed DNA Extrac�on
Forensic DNA
Extrac�on
TANBead® Nucleic Acid Extrac�on Kit (6TF) is
dedicated to isolate DNA from small sample
quan�ty or challenging sizes. Samples need to be
treated by following the prepara�on steps,
incubated in incuba�on buffer, and treated with
Proteinase K and DTT (Dithiothreitol). The lysates
need to be added into Auto Plate or Auto Tube
and automa�cally processed using TANBead®
Nucleic Acid Extractor. The highly extracted and
purified DNA can be directly applied for various
applica�ons, including real-�me PCR, short
tandem repeat (STR) analysis, mitochondrial DNA
(mtDNA) analysis and other forensic tests.
Introduc�on
TANBead® Forensic DNA Kit
Specifica�on
Samples
Reagent kits
Extrac�on system
Applica�ons
Opera�on �me
PCR and qPCR
cigare�e, hair, blood stain,
dried blood spot, semen stain,
chewing gun, nail
55 - 75 min
6TF series
Maelstrom 8 /
Maelstrom 48 series
Maelstrom 96 series
Key features
Automated magne�c beads-based
nucleic acids extrac�on technology
Purify limited nucleic acids from various
forensic sample types
Provide choices for different sample
inputs, such as 8, 48, 96 tests per rum
Figure 1.
The qPCR results of GAPDH and ACTIN (ACTB). The
nucleic acid was extracted from various samples using
TANBead® Forensic DNA Extrac�on Kit. Each sample
type was replicated three �mes.
Figure 2.
Agarose gel electrophoresis of nucleic acids extracted
from seven different sample types with TANBead®
Forensic DNA Extrac�on Kit. Mitochondrial DNA was
amplified with PCR (Product size: 1200 bp) and 50 μL of
each sample was loaded on a 1% agarose gel. Marker:
Bio-1kbTM Mass DNA Ladder; NC: Nega�ve control;
Lane 1: Blood stain; Lane 2: Dried blood spot; Lane 3:
Semen stain; Lane 4: Nail; Lane 5: Hair follicles; Lane 6:
Chewing gum; Lane 7: Cigare�e bu�s.
Extrac�on on Maelstrom 4800
1000 bp -
Extrac�on on Maelstrom 4800
N
a
i
l
0
10
20
30
40
Ct
Value
of
GAPDH
B
l
o
o
d
S
t
a
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n
S
e
m
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S
t
a
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H
a
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F
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l
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C
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a
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e
�
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B
u
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s
D
r
i
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d
B
l
o
o
d
S
p
o
t
Extrac�on on Maelstrom 4800
N
a
i
l
0
10
20
30
40
Ct
Value
of
ACTB
B
l
o
o
d
S
t
a
i
n
S
e
m
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n
S
t
a
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H
a
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F
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l
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B
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D
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B
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S
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Forensic DNA Extrac�on
TANBead® RT-LAMP SARS-CoV-2 detec�on kit is
designed for rapid detec�on of purified nucleic
acids containing SARS-CoV-2. The purified
nucleic acids can be obtained from swabs,
saliva, serum, and urine specimens using
TANBead® Nucleic acids extrac�on kits (665 and
685 series, page 16~18). Results are monitored
by fluorescent signal and indicates SARS-CoV-2
RNA presence. This assay is based on the
Loop-mediated Isothermal Amplifica�on
(LAMP) reac�on, which is one of the Nucleic
Acid Amplifica�on Tests (NAATs). The reac�on
u�lizes a set of primers to rapidly amplify the
specific DNA fragment at 65°C using reverse
transcriptase and recombinant Bst DNA
polymerase. This assay is very �me-efficient and
sensi�ve, which can give results within 30
minutes, and strong posi�ve samples can be
observed as soon as 10 minutes.
Introduc�on
RT-LAMP
Detec�on
TANBead® RT-LAMP Kit
Specifica�on
Reagent kits
Applica�ons
Time-to-result
SARS-CoV-2 detec�on
30 minutes
Samples Purified nucleic acid
System Real-�me PCR instruments
TANBead® RT-LAMP kit
Key features
Highly sensi�ve nucleic acid detec�on
Isothermal amplifica�on
Simple and low-cost equipment
Rapid process typically less than 30
minutes
Figure 1.
Comparison of RT-LAMP and RT-qPCR for the detec�on
of serial-diluted standard SARS-CoV-2 RNA samples. A
10-fold serial dilu�on of SARS-CoV-2 RNA star�ng from
107
to 10 copies per reac�on was added into TANBead®
RT-LAMP reagents or D-brand detec�on kit. SARS-CoV-2
detec�on with (A) TANBead® RT-LAMP at 65°C or (B)
D-brand detec�on kit was performed in CFX96™
Real-Time PCR Detec�on System. The rela�ve fluores-
cence units (RFU) indicated the presence of SARS-CoV-2
N gene nucleic acid in samples. The Ct values of each
dilutant detected with D-brand kit, star�ng from 107
to
10 copies and nega�ve control, were 15.06, 18.61,
21.85, 25.06, 28.46, 21.18, 36.97 and ND, respec�vely.
Meanwhile, Ct values of dilutants detected with
TANBead® RT-LAMP kit were much more compe��ve.
Figure 2.
Detec�on of SARS-CoV-2 RNA via real-�me RT-LAMP.
Time-to-results were demonstrated using low, medium,
and high RNA input (each n = 4), where the amount of
RNA from each clinical sample was pre-determined with
RT-qPCR. Regardless of the input amount, TANBead®
RT-LAMP amplified SARS-CoV-2 RNA in less than 20
minutes. No template control (NTC) signal was not
detected even a�er 40 minutes of assay.
TANBead
L brand
High RNA
input
Medium RNA
input
Low RNA
input
NTC
Time
to
result
(min)
20
30
40
10
0
RFU
0 5 10 25 30
20
15
0
1000
2000
3000
4000
5000
6000
7000
8000
9000
Amplifica�on curve of RT-LAMP
Time(min)
RFU
Amplifica�on curve of RT-qPCR
Cycles
0
500
1000
1500
2000
0 40
30
10 20
(A)
(B)
RT-LAMP Detec�on
mitochondrial DNA
gDNA
Sample Blood stain Chewing gum Cigare�e bu�s Sperm Stamps, Envelopes Fingernails Hair, Hair roots
B
lo
o
d
D
N
A
(
6
1
1
)
B
l
o
o
d
R
N
A
(
6
2
1
)
F
F
P
E
D
N
A
(
6
1
P
)
H
P
V
D
N
A
(
6
1
H
)
P
la
n
t
D
N
A
(
6
1
3
)
P
la
n
t
R
N
A
(
6
K
3
)
T
i
s
s
u
e
D
N
A
(
6
T
2
)
T
is
s
u
e
R
N
A
(
6
K
2
)
S
t
o
o
l
D
N
A
(
6
S
C
)
F
u
n
g
i
(
6
1
F
)
S
T
I
(
6
S
T
)
H
B
V
(
6
1
5
)
O
p
�
P
u
r
e
B
lo
o
d
D
N
A
(
6
1
E
)
O
p
�
P
u
r
e
c
f
D
N
A
(
6
1
C
)
D
r
ie
d
B
lo
o
d
S
p
o
t
(
6
1
E
-
B
S
)
O
p
�
P
u
r
e
V
ir
a
l
(
6
6
5
)
V
ir
a
p
id
V
i
r
a
l
(
6
8
5
)
P
la
n
t
D
N
A
(
6
1
3
-
S
E
)
B
a
c
t
e
r
ia
D
N
A
(
6
1
G
)
B
a
c
t
e
r
ia
D
N
A
(
6
1
3
-
S
E
)
Sample Target
gDNA
Total RNA
Bacteria
Parasite
Virus
gDNA
Total RNA
gDNA
Total RNA
gDNA
Bacteria
Virus
cfDNA
gDNA
Bacteria
Virus
gDNA
Bacteria
Virus
Bacteria
Virus
Bacteria
Bacteria
Virus
gDNA
Total RNA
Bacteria
Bacteria
Virus
gDNA
Bacteria
Virus
gDNA
Total RNA
Bacteria
Virus
gDNA
Total RNA
gDNA
gDNA
Bacteria
Virus
gDNA
Bacteria
Virus
gDNA
Bacteria
Virus
gDNA
Total RNA
gDNA
gDNA
Total RNA
Bacteria
Virus
Blood
Buffy coat
Plasma / Serum
Bone marrow
Body fluids
BAL, aspirates
Bacterial culture
Cultured cells
Sputum
Saliva
Solid �ssue
Rodent tails
FFPE
Swab
Urine
Stool
Fish
Yeast
Plant: seeds, leaves
Water
Cell culture
supernatant
Liquid sample
transport media
Leukocyte
Clo�ed, Dried blood
2
4
1
1
3
Note. 1. Wang L, Yang S, Yan X, et al. Comparing the yield of oropharyngeal swabs and sputum for detec�on of 11 common pathogens in hospitalized children with lower
respiratory tract infec�on. Virol J. 2019 Jun 24:16(1):84.
2. Yu RL, Tu SC, Wu RM, et al. Interac�ons of COMT and ALDH2 Gene�c Polymorphisms on Symptoms of Parkinson’s Disease. Brain Sci. 2021 Mar 12;11(3):361.
3. Hsu JC, Huang, H, Lin H, et al. Applying Modified VP53A Recombinant Protein as an An�-White Spot Syndrome Virus Biological Agent in Litopenaeus vannamei
Farming. Viruses 2022 Jun 21;14:1353.
4. Tsai K, Tu Y, Wu C, et al. First detec�on and phylogene�c analysis of lumpy skin disease virus from Kinmen Island, Taiwan in 2020. The Journal of Veterinary Medical
Science 2022 Jun 13;21-0649.
Kit Reference
TANBead Forensic DNA
Recommended: Internally Validated kit with proven extrac�on efficiency on the sample type and target.
Compa�ble: Can be used on the sample type and target. Not internally validated - Extrac�on efficiency and results
may differ.
User-valida�on sample type and target.
Kit Reference
Reagent Kits for : Maelstrom 8 Autostage, Maelstrom 4800 and Maelstrom 4810
301126
301127
301128
301307
301129
301400
301401
301134
301371
301135
301372
301383
301384
301385
301389
301152
301153
301148
301149
301346
301572
301573
301431
301432
301130
301131
301132
301306
301133
301260
301366
301367
301150
301151
301424
301425
301257
301138
301294
301139
301295
M611A46
M611S46
M61EA46
M61EA10
M61ES46
M621A46
M621S46
M613A46*
M613A46-SE*
M613S46*
M613S46-SE*
M6K3A46*
M6K3S46*
M61CA46
M61CS46
M61PA46
M61PS46
M665A46
M665S46
M665A10
M685A46
M685S46
M61HA46
M61HS46
M612A46
M612S46
M6T2A46
M6T2A10
M6T2S46
M6T2046
M6K2A46
M6K2S46
M61FA46
M61FS46
M6TFA46
M6TFS46
M61G046
M61GA46
M61GA46-SE
M61GS46
M61GS46-SE
TANBead Blood DNA Auto Plate
TANBead Blood DNA Auto Tube
TANBead Op�Pure Blood DNA Auto Plate
TANBead Op�Pure Blood DNA Bulk Plate
TANBead Op�Pure Blood DNA Auto Tube
TANBead Blood RNA Auto Plate
TANBead Blood RNA Auto Tube
TANBead Plant RNA Auto Plate
TANBead Plant RNA Auto Tube
TANBead Op�Pure cfDNA Auto Plate
TANBead Op�Pure cfDNA Auto Tube
TANBead Op�Pure FFPE DNA Auto Plate
TANBead Op�Pure FFPE DNA Auto Tube
TANBead Op�Pure Viral Auto Plate
TANBead Op�Pure Viral Auto Tube
TANBead Op�Pure Viral Bulk Plate
TANBead Virapid Virus Auto Plate
TANBead Virapid Virus Auto Tube
TANBead HPV Auto Plate
TANBead HPV Auto Tube
TANBead Tissue DNA Auto Plate
TANBead Tissue DNA Auto Tube
TANBead Tissue Total DNA Auto Plate
TANBead Tissue Total DNA Bulk Plate
TANBead Tissue Total DNA Auto Tube
TANBead Tissue Total DNA Auto Kit
TANBead Tissue RNA Auto Plate
TANBead Tissue RNA Auto Tube
TANBead Fungi DNA Auto Plate
TANBead Fungi DNA Auto Tube
TANBead Forensic DNA Auto Plate
TANBead Forensic DNA Auto Tube
TANBead Gram Bacteria DNA Auto Kit
TANBead Gram Bacteria DNA Auto Plate
TANBead Gram Bacteria DNA Auto Tube
96
96
96
960
96
96
96
96
96
96
96
96
96
96
96
96
96
96
96
960
96
96
96
96
96
96
96
960
96
96
96
96
96
96
96
96
96
96
96
96
96
Sample Descrip�on Test Reference No. Ordering No.
Blood
Plant
cfDNA
FFPE
HPV
Virus
Tissue
Bacteria
Fungi
Forensic
Plasmid
TANBead Plant DNA Auto Plate
TANBead Plant DNA Auto Tube
301578
301579
M6PEA46*
M6PES46*
TANBead Plasmid Extrac�on Auto Plate
TANBead Plasmid Extrac�on Auto Tube
96
96
Note: black font = IVD and RUO available
blue font = RUO available
Ordering Informa�on
Reagent Kits for : Maelstrom 8 Autostage, Maelstrom 4800 and Maelstrom 4810
Reagent Kits for : Maelstrom 9600 and Maelstrom 9610
301186
301187
301188
301189
301402
301403
301194
301379
301259
301378
301406
301407
301377
301386
301212
301213
301224
301345
301209
301574
301575
301591
301592
301190
301191
301192
301193
301404
301405
W611A46
W611S66
W61EA46
W61ES66
W621A46
W621S66
W613A46*
W613A46-SE*
W613S66*
W613S66-SE*
W6K3A46*
W6K3S66*
W61CA46
W61CS66
W61PA46
W61PS66
W665A46
W665A10
W665S66
W685A46
W685S66
W61HA46
W61HS66
W612A46
W612S66
W6T2A46
W6T2S66
W6K2A46
W6K2S66
TANBead Blood DNA Auto Plate
TANBead Blood DNA Auto Tube
TANBead Op�Pure Blood DNA Auto Plate
TANBead Op�Pure Blood DNA Auto Tube
TANBead Blood RNA Auto Plate
TANBead Blood RNA Auto Tube
TANBead Plant RNA Auto Plate
TANBead Plant RNA Auto Tube
TANBead Op�Pure cfDNA Auto Plate
TANBead Op�Pure cfDNA Auto Tube
TANBead Op�Pure FFPE DNA Auto Plate
TANBead Op�Pure FFPE DNA Auto Tube
TANBead Op�Pure Viral Auto Plate
TANBead Op�Pure Viral Bulk Plate
TANBead Op�Pure Viral Auto Tube
TANBead Virapid Virus Auto Plate
TANBead Virapid Virus Auto Tube
TANBead HPV DNA Auto Plate
TANBead HPV DNA Auto Tube
TANBead Tissue DNA Auto Plate
TANBead Tissue DNA Auto Tube
TANBead Tissue Total DNA Auto Plate
TANBead Tissue Total DNA Auto Tube
TANBead Tissue RNA Auto Plate
TANBead Tissue RNA Auto Tube
96
72
96
72
96
72
96
96
72
72
96
72
96
72
96
72
96
960
72
96
72
96
72
96
72
96
72
96
72
Sample Descrip�on Test Reference No. Ordering No.
Blood
Plant
cfDNA
FFPE
Virus
Tissue
301635
301636
301387
301388
301414
301415
M6GMA46*
M6GMS46*
M6SCA46
M6SCS46
M6STA46
M6STS46
TANBead Food and Feed DNA Auto Plate
TANBead Food and Feed DNA Auto Tube
TANBead Stool Cell DNA Auto Plate
TANBead Stool Cell DNA Auto Tube
TANBead STIs Extrac�on Auto Plate
TANBead STIs Extrac�on Auto Tube
96
96
96
96
96
96
Sample Descrip�on Test Reference No. Ordering No.
Food and Feed
Stool
STIs
Note: black font = IVD and RUO available
blue font = RUO available
Tissue
TANBead Plant DNA Auto Plate
TANBead Plant DNA Auto Tube
Note: black font = IVD and RUO available
blue font = RUO available
HPV
Ordering Informa�on
Reagent Kits for : Maelstrom 9600 and Maelstrom 9610
Reagent Kits for : Maelstrom 2400 and Maelstrom 2410
301411
301631
L91C045
L91E045*
TANBead Op�Pure cfDNA Auto Kit
TANBead Blood DNA Auto Kit
48
48
cfDNA
Blood
Sample Descrip�on Test Reference No. Ordering No.
Note: black font = IVD and RUO available
blue font = RUO available
Sample Descrip�on Test Reference No.
Note: black font = IVD and RUO available
blue font = RUO available
Ordering No.
W6TFA46
W6TFS66
W61GA46
W61GS66
W6PEA46*
W6PES66*
W6GMA46*
W6GMS66*
W6SCA46
W6SCS66
W6STA46
W6STS66
TANBead Forensic DNA Auto Plate
TANBead Forensic DNA Auto Tube
TANBead Gram Bacteria DNA Auto Plate
TANBead Gram Bacteria DNA Auto Tube
TANBead Plasmid Extrac�on Auto Plate
TANBead Plasmid Extrac�on Auto Tube
TANBead Food and Feed DNA Auto Plate
TANBead Food and Feed DNA Auto Tube
TANBead Stool Cell DNA Auto Plate
TANBead Stool Cell DNA Auto Tube
TANBead STIs DNA Auto Plate
TANBead STIs DNA Auto Tube
96
72
96
72
96
72
96
72
96
72
96
72
Bacteria
Plasmid
Food and Feed
Stool
STIs
301291
301426
301198
301199
301580
301581
301637
301638
301392
301391
301595
301596
Forensic
Ordering Informa�on

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Brochure_ Reagent Kits Ver.6.pdf

  • 1. Reagents Manufacturer TOTAL SOLUTION FOR NUCLEIC ACID EXTRACTION
  • 2. TANBead Blood DNA Kit is designed for rapid, reliable, automated purifica�on of DNA from blood samples. Our magne�c beads-based technology with our corresponding extrac�on system can provide you automated, high-throughput and easy-to-use nucleic acids extrac�on. The extracted nucleic acids can be applied for various applica�ons, such as PCR, qPCR, HLA-typing, and sequencing. Introduc�on Key features TANBead® Blood DNA Kit Blood DNA Extrac�on Automated magne�c beads-based nucleic acids extrac�on technology High yield and high-quality nucleic acids Provide choices for different sample inputs, such as 8, 48, 96 tests per run Table 1. Figure 1. 10 kb - Whole blood #1 #2 #3 #1 #2 #3 #1 #2 #3 Frozen blood Buffy coat 3 kb - 1 kb - Integrity of DNA extracted from whole blood, frozen blood and buffy coat using 61E kit, examined through gel electrophoresis. Yield and quality of extracted DNA from 300 μL whole blood samples using 61E kit. Yield (μg) 4.15 1.93 0.02 0.21 Quality A260/A280 Mean SD Specifica�on Samples Reagent kits Extrac�on system Applica�ons Opera�on �me PCR-based HLA-typing, and NGS analysis Whole blood, frozen blood, buffy coat 50 - 60 min 61E series Maelstrom 8 / Maelstrom 48 series / Maelstrom 96 series Blood DNA Extrac�on
  • 3. Table 1. Figure 1. Figure 2. TANBead Blood RNA Kit is designed for rapid, reliable, automated purifica�on of RNA from blood samples. Our magne�c beads-based technology with our corresponding extrac�on system can provide you automated, high-throughput and easy-to-use nucleic acids extrac�on. The extracted nucleic acids can be applied for various applica�ons, such as real-�me PCR and RT-PCR. Introduc�on TANBead® Blood RNA Kit Blood RNA Extrac�on Specifica�on Samples Reagent kits Extrac�on system Applica�ons Opera�on �me RT-PCR and RT-qPCR Whole blood 45 - 55 min 621 series Maelstrom 8 / Maelstrom 48 series / Maelstrom 96 series Key features Purify RNA from the whole blood sample High yield and high-quality nucleic acids Provide choices for different sample inputs, such as 8, 48, 96 tests per run Quan�fica�on Amplifica�on Plot cycle untreated 1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 treated Rn R2 = 0.9989 Log of template dilu�on 35 30 25 20 -4 -3 -2 -1 0 15 Cq (B) (A) The GAPDH products were successfully amplified from the extracted RNA, along with its 10-fold serial dilu�ons. (B) The linear rela�onship of Cq values of each dilu�ons was demonstrated. The mean Cq values of the samples were 20.22 ± 0.18, 23.52 ± 0.15, 26.00 ± 0.14, 29.11 ± 0.14, and 31.93 ± 0.43. RNA in the extracted nucleic acid, observed by examin- ing the GAPDH expression levels in the presence or absence of DNase I treatment. The mean Cq value of untreated group is 28.83 ± 0.67, and that of treated group is 29.08 ± 0.45. Yield and quality of extracted RNA from 100 μL whole blood samples using 621 kit. Yield (ng) 8.66 2.00 0.10 0.16 Quality A260/A280 Mean SD 1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 Rn Quan�fica�on Amplifica�on Plot cycle (A) 1x d10x d102x d103x d104x Blood RNA Extrac�on
  • 4. Table 1. (B) Plasma (A) Serum TANBead cfDNA Kit is designed for rapid, reliable, automated purifica�on of cfDNA from plasma and serum samples. Our magne�c beads-based technology with our correspond- ing extrac�on system can provide you automat- ed, high-throughput and easy-to-use nucleic acids extrac�on. The extracted nucleic acids can be applied to various applica�ons, such as PCR, qPCR, and sequencing for oncology applica�on. Introduc�on cfDNA Extrac�on TANBead® cfDNA Kit Specifica�on Samples Reagent kits Extrac�on system Applica�ons Opera�on �me PCR, qPCR and NGS anaylsis Serum or plasma 55 - 70 min 61C series, L91C Maelstrom 8 / Maelstrom 24 series / Maelstrom 48 series / Maelstrom 96 series Key features Automated magne�c beads-based nucleic acids extrac�on technology High yield and high-quality nucleic acids Provide choices for different sample inputs, such as 8, 24, 48, 96 tests per run Yield and integrity measurement of extracted cfDNA from 4mL serum or plasma samples using L91C kit on Maelstrom 24 series. Figure 1. The fragment size (red arrow) of extracted cfDNA from the serum (A) or plasma (B) samples were examined with capillary electrophoresis. RFU 23.40 18.92 14.45 9.98 5.51 20 100 200 Length(bp) 300 400500600 1k 2k 3k 1.03 RFU 21.95 17.76 13.56 9.37 5.18 20 100 200 Length(bp) 300 400500600 1k 2k 3k 0.99 Sample Serum Plasma 20.3 1.43 0.49 0.2 2.56 88.9 Integrity yield (ng) Alu115 (ng) Alu247/ Alu115 cfDNA Extrac�on
  • 5. Log IU/ml TANBead y=0.9862x-0.2143 R 2 =0.9637 8.00 7.00 6.00 5.00 4.00 3.00 2.00 1.00 A 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 Ct ΔRn 10⁶ IU 10⁵ IU 10⁴ IU 10³ IU 10² IU Threshold (A) Swab Quan�fica�on Amplifica�on Plot 1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 41 43 1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 Ct ΔRn 10⁶ IU 10⁵ IU 10⁴ IU 10³ IU 10² IU Threshold (B) Sputum Quan�fica�on Amplifica�on Plot 1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 Ct ΔRn 10⁶ IU 10⁵ IU 10⁴ IU 10³ IU 10² IU Threshold (C) BAL Quan�fica�on Amplifica�on Plot The virus fragment was stably amplified in the extracted RNA that isolated from samples containing various concentra�on of HCV standard template. The sample types, including swab (A), sputum (B) and BAL (C) were examined. Figure 1. (A) (B) Figure 2. 1000 800 600 400 200 0 0 20 Amplfica�on 40 10 30 125 copies E gene Commercial-S TANBead RFU 500 400 300 200 100 0 0 20 Amplfica�on 40 10 30 125 copies RFU RdRP gene Commercial-S TANBead 1000 800 600 400 200 0 0 20 Amplfica�on 40 10 30 125 copies RFU N gene Commercial-S TANBead 1000 800 600 400 200 0 0 20 Amplfica�on 40 10 30 125 copies RFU IC Commercial-S TANBead TANBead Virus DNA/RNA Kit is designed for rapid, reliable, automated purifica�on of nucleic acids from various sample types. Our magne�c beads-based technology with our correspond- ing extrac�on system can provide you automat- ed, high-throughput and easy-to-use nucleic acids extrac�on. The extracted nucleic acids can be applied for various applica�ons, such as PCR, qPCR, RT-PCR, RT-qPCR and sequencing. Introduc�on Key features TANBead® Virus DNA/RNA Kit Virus DNA/RNA Extrac�on Automated magne�c beads-based nucleic acids extrac�on technology High yield and high-quality nucleic acids Provide choices for different sample inputs, such as 8, 48, 96 tests per run Specifica�on Samples Reagent kits Extrac�on system Applica�ons Opera�on �me PCR, qPCR and sequencing Serum, plasma, swabs, sputum, or bronchoalveolar lavage (BAL) 35 - 45 min 665 series Maelstrom 8 / Maelstrom 48 series / Maelstrom 96 series (A) In sixty HCV posi�ve samples, high correla�on between TANBead viral extrac�on kit and commercial-A all-in-one sample prepara�on and detec�on system was demonstrated (X-axis: Log IU/ml of HCV RNA extracted by the 665 kit Y-axis: Log IU/ml of HCV RNA extracted by commercial-A sample prepara�on system) (B) samples spiked with 125 copies of COVID-19 pseudovirus were extracted using 665 or com- mercial S kit and were subject- ed to qPCR analysis for N, E, RdRP, and internal control (IC). Virus DNA/RNA Extrac�on
  • 6. TANBead Virapid Viral Extrac�on Kit is designed for those who were struggling to isolate nucleic acids from viral samples. Our technology provides the solu�on to complete the whole extrac�on processes in about 15 minutes. The extracted nucleic acids can be applied to applica�ons, such as PCR, qPCR and RT-PCR. Introduc�on Key features TANBead® Virapid Virus Kits Virapid Virus Extrac�on Simply transfer the sample to the pre-filled plate/tube for extrac�on without addi�onal proteinase K Ultra-fast process which takes only about 15 minutes Provide choices for different sample inputs, such as 8, 48, 96 tests per run Specifica�on Samples Reagent kits Extrac�on system Applica�ons Opera�on �me PCR, qPCR, RT-PCR Nasal, nasopharyngeal, or oropharyngeal swab, saliva, urine 14 - 17 min 685 series Maelstrom 8 / Maelstrom 48 series / Maelstrom 96 series The automa�c extrac�on opera�on �me of 685 series kits were shown as below: 16.33 min (M9600), 14.23 min (M4800), 14.31 min (M8). Figure 1. Figure 2. The extrac�on performance of the M685A46 kit was examined by three qPCR kits, (A) Vircell, (B) Seegene, (C) SolGent, and the results indicated that M685A46 kit's extrac�on performance is comparable to M665A46 kit. COVID-19 pseudovirus (500 copies) spiked in virus transporta�on medium (VTM) were used as the extrac�on samples. (A) Vircell Direct SARS-CoV-2 qPCR kit N gene E gene RNaseP Target gene Ct value 30 35 40 25 20 665 series 685 series 665 series 685 series N gene E gene RdRP (B) Seegene AllPlex 2019-nCoV Assay Target gene Ct value 30 35 40 25 20 Minute Opera�on Time M8 M4800 M9600 0 20 40 665 series 685 series Virapid Virus Extrac�on
  • 7. Figure 3. Saliva and urine samples were spiked with 500 copies of COVID-19 pseudovirus. Samples were extracted with M685A46 kit and results showed that the kit can extract viral nucleic acid from saliva and urine. (C) SolGent DialPlexQ 2019-nCoV Detec�on kit 665 series 685 series N gene ORF1a PCRC Target gene Ct value 30 35 40 25 20 Saliva Urine Vircell Direct SARS-CoV-2 qPCR kit 30 35 40 25 20 N gene E gene RNaseP Figure 4. Eight clinical samples were subjected to nucleic acids extrac�on using 685 and 665 kits, and the extracts was analyzed with Vircell - DIRECT SARS-COV-2 REALTIME PCR KIT. Results showed high correla�on between 685 and 665 kit's Ct values of extracted N,E and RNaseP genes. The detec�on performance correla�on of clinical sample by 685 and 665 kits 20 20 25 25 30 30 35 35 685 Ct Value 665 Ct Value 40 40 45 45 N gene E gene RNaseP N gene E gene RNaseP 0.982 0.985 0.978 Ct value correla�on between 2 kits Virapid Virus Extrac�on
  • 8. The TANBead HPV DNA Nucleic Acid Extrac�on Kit is designed to perform the HPV nucleic acids extrac�on. By using TANBead Nucleic Acid Extrac�on Systems, the one-step-to-extrac�on can be performed automa�cally. Cervical swabs or liquid based cytology samples are processed through a series of automa�c extrac�on steps and the high-quality nucleic acids can be applied directly for further applica�ons. The extracted nucleic acids can be applied for qualita�ve and quan�ta�ve molecular analyses, such as real-�me PCR. Introduc�on HPV DNA Extrac�on TANBead® HPV DNA Kit Specifica�on Samples Reagent kits Extrac�on system Applica�ons Opera�on �me PCR and qPCR cervical swab, liquid based cytology samples 35 - 45 min 61H series Maelstrom 8 / Maelstrom 48 series Maelstrom 96 series Key features Automated magne�c beads-based nucleic acids extrac�on technology and prefilled reagent system Mucus specimens are applicable through a simple pretreatment step Provide choices for different sample inputs, such as 8, 48, 96 tests per run Virus fragments were stably extracted and amplified from samples containing various concentra�ons of cervix cells with human papillomavirus type 16 genome. The sample types including (a) cervical swab (b) liquid based cytology, were examined. Figure 1. The 61H kit can stably perform nucleic acids extrac�on from both mucus and non-mucus specimens. The SiHa cells containing HPV 16 genome, or the RWPE-1 cells containing HPV 18 genome were subjected to nucleic acids extrac�on and qPCR analysis. The Ct value results of mucus samples were similar to those of non-mucus samples. Figure 2. Non-mucus Pre-treatment Non-mucus Mucus Mucus HPV 18 HPV 16 Ct value 28 + - + - 30 32 34 RFU 0 0 10 20 30 40 50 500 1000 1500 2000 2500 3000 (b) liquid based cytology Amplifica�on 105 104 103 102 Cycles Threshold RFU 0 0 10 20 30 40 50 500 1000 1500 2000 2500 3000 (a) cervical swab Amplifica�on 105 104 103 102 Cycles Threshold HPV DNA Extrac�on
  • 9. Table 1. Clinical inves�ga�on result of HPV-posi�ve or nega�ve clinical specimens (stored in ThinPrep) for HPV type 16,18 or 45 detec�ons, using 61H kits for nucleic acids extrac�on. These specimens were purchased and have been validated for HPV types using Hologic HPV detec�on kit. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND 33.08 25.64 23.49 25.08 29.19 25.13 27.82 32.37 26.31 23.29 28.46 31.42 23.15 30.29 29.35 34.28 30.96 32.55 28.22 27.95 41.3 30.64 33.8 37.56 25.3 25.15 20.29 30.98 S00880598 S00880599 S00880600 S00880601 S00880602 S00880603 S00880604 S00880605 S00880606 S00880607 S00880608 S00880609 S00880610 S00880611 S00880612 S00880613 S00880614 S00880615 S00880616 S00880617 S00880648 S00880649 S00880650 S00880652 S00880653 S00880654 S00880655 S00880656 HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) HPV 16/18/45 (-) S000880625 S000880626 S000880627 S000880630 S000880631 S000880632 S000880635 S000880636 S000880637 S000880640 S000880641 S000880642 S000880645 S000880647 S000880618 S000880619 S000880620 S000880621 S000880622 S000880623 S000880624 S000880628 S000880629 S000880633 S000880634 S000880638 S000880639 S000880643 HPV 16 (+) HPV 16 (+) HPV 16 (+) HPV 16 (+) HPV 16 (+) HPV 16 (+) HPV 16 (+) HPV 16 (+) HPV 16 (+) HPV 16 (+) HPV 16 (+) HPV 16 (+) HPV 16 (+) HPV 16 (+) HPV 45 (+) HPV 18 (+) HPV 18 (+) HPV 18 (+) HPV 18 (+) HPV 18 (+) HPV 45 (+) HPV 18 (+) HPV 45 (+) HPV 45 (+) HPV 45 (+) HPV 18 (+) HPV 18 (+) HPV 45 (+) Nega�ve sample Remarks Remarks Posi�ve sample Ct value Ct value N.O. HPV DNA Extrac�on
  • 10. Table 1. Figure 1. The yield and quality of extracted DNA from 106 Salmo- nella or Staphylococcus using the 61G kit. Genomic DNA from 14 gram-posi�ve and gram-nega- �ve bacteria is well isolated using the 61G kit. TANBead Bacteria DNA Kit is designed for rapid, reliable, automated purifica�on of nucleic acids from gram(-), gram(+) and other atypical bacteria samples. Our magne�c beads-based technology with our corresponding extrac�on system can provide you automated, high-throughput and easy-to-use nucleic acids extrac�on. The extracted nucleic acids can be applied for various applica�ons, such as PCR, qPCR, and sequencing. Introduc�on Key features TANBead® Bacteria DNA Kit Bacteria DNA Extrac�on Automated magne�c beads-based nucleic acids extrac�on technology High yield and high-quality nucleic acids Provide choices for different sample inputs, such as 8, 48, 96 tests per run Specifica�on Samples Reagent kits Extrac�on system Applica�ons Opera�on �me PCR, qPCR, and sequencing Sputum, bronchoalveolar lavage (BAL), or cultured bacteria 61G (70 - 80 min) 61G-SE (45 - 55 min) 61G series, 61G-SE series Maelstrom 8 / Maelstrom 48 series / Maelstrom 96 series 1: Bacillus 2: Microbacterium 3: Massilia 4: Paenibacillus 5: Corynebacterium 6: Escherichia 7: Sphingomonas 8: Cupriavidus 9: Duganella 10: Flavobacterium 11: Lactobacillus 12: Weissella 13: Leuconostoc 14: Burkholderia 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Salmonella Staphylococcus Mean Mean SD 33.1 0.8 0.21 0.04 2.04 0.02 2.06 34.3 SD Yield (μg) Quality A260/A280 - gDNA Bacteria DNA Extrac�on
  • 11. Figure 2. Five cerebrospinal fluid (CSF) clinical samples with unknown pathogen infec�on were subjected to nucleic acids extrac�on and the extracts were analyzed through qPCR analysis to detect N. meningitidis (bacteria) and C. neoformans (fungi). The results demonstrated that 61G kit is applicable for nucleic acids extrac�on from CSF samples. Figure 3. Eight liquid culture samples of M. tuberculosis were subjected to nucleic acids extrac�on and the extracts were analyzed with qPCR analysis. The results demonstrated that 61G kit is applicable for nucleic acids extrac�on of TB liquid culture samples. N. meningitidis C. neoformans Target qPCR results (shown as Ct value) wl-35663 wl-38969 CSF clinical sample #01 CSF clinical sample #02 CSF clinical sample #03 CSF clinical sample #04 CSF clinical sample #05 CN4 CN5 36.43 ND 45.13 ND ND ND 38.27 ND 33.86 37.62 Home-made Primer set Liquid culture #01 19.7 Liquid culture #02 20.67 Liquid culture #03 21.28 Liquid culture #04 20.78 Liquid culture #05 23.06 Liquid culture #06 17.35 Liquid culture #07 21.14 Liquid culture #08 18.81 Posi�ve control (300 copies) 21.91 NTC ND qPCR results (shown as Ct value) Target M. tuberculosis Bacteria DNA Extrac�on
  • 12. Yield and quality of extracted DNA from meat, zebraf- ish, or cells using 6T2 kit. Confirma�on of DNA integrity extracted from meat, zebrafish or leukocyte using 6T2 kit, examined through gel electrophoresis. Table 1. Figure 1. TANBead Tissue DNA Kit is designed for rapid, reliable, automated purifica�on of DNA from �ssues and cells. Our magne�c beads-based technology with our corresponding extrac�on system can provide you automated, high-throughput and easy-to-use nucleic acids extrac�on. The extracted nucleic acids can be applied for various applica�ons, such as PCR, qPCR, and sequencing. Introduc�on Tissue DNA Extrac�on TANBead® Tissue DNA Kit Specifica�on Samples Reagent kits Extrac�on system Applica�ons Opera�on �me PCR, qPCR and Southern blot Tissues or cells 70 - 80 min 6T2 series Maelstrom 8 / Maelstrom 48 series / Maelstrom 96 series Key features Automated magne�c beads-based nucleic acids extrac�on technology High yield and high-quality nucleic acids Provide choices for different sample inputs, such as 8, 48, 96 tests per run 10 kb - 3 kb - 1 kb - Meat #1 #2 #1 #2 #1 #2 Zebrafish Leukocyte 31.09 Yield (μg) Quality A260/A280 0.61 29.29 0.54 22.52 0.01 Mean SD Mean SD Mean SD 1.81 0.02 1.8 0 1.98 0.51 50 mg meat 50 mg zebrafish 106 cells Tissue DNA Extrac�on
  • 13. Figure 1. Table 1. Integrity of RNA from 30 mg zebrafish, 30 mg meat and 106 THP-1 or SHSY5Y cells extracted with 6K2 kit, exam- ined through gel electrophoresis. Yield and quality of extracted RNA from zebrafish, meat and cells (THP-1 or SHSY5Y) using 6K2 kit. TANBead Tissue RNA Kit is designed for rapid, reliable, automated purifica�on of RNA from �ssues and cells. Our magne�c beads-based technology with our corresponding extrac�on system can provide you automated, high-throughput and easy-to-use nucleic acids extrac�on.The extracted nucleic acids can be applied for various applica�on, such as RT-PCR. Introduc�on Tissue RNA Extrac�on TANBead® Tissue RNA Kit Specifica�on Samples Reagent kits Extrac�on system Applica�ons Opera�on �me RT-PCR,qRT-PCR and Northern blot Tissues or cells 45 - 60 min 6K2 series Maelstrom 8 / Maelstrom 48 series / Maelstrom 96 series Key features Automated magne�c beads-based nucleic acids extrac�on technology High yield and high-quality nucleic acids Provide choices for different sample inputs, such as 8, 48, 96 tests per run Yield (μg) Quality A260/A280 Zebrafish Meat THP-1 SHSY5Y 15.21±1.12 12.12±0.65 18.34±0.81 26.29±1.05 1.94±0.01 1.95±0.03 1.98±0.01 1.99±0.02 Meat #1 #2 #1 #2 Zebrafish 3 kb - 1 kb - 0.2 kb - THP-1 #1 #2 #3 #3 #4 #1 #4 #2 SHSY5Y 2 kb - 1 kb - 0.2 kb - Tissue RNA Extrac�on
  • 14. TANBead FFPE DNA Kit is designed for rapid, reliable, automated purifica�on of DNA from FFPE samples. Our magne�c beads-based technology with our corresponding extrac�on system can provide you automated, high-throughput and easy-to-use nucleic acids extrac�on. The extracted nucleic acids can be applied for various applica�on, such as PCR, qPCR, and sequencing. Introduc�on FFPE DNA Extrac�on TANBead® FFPE DNA Kit Specifica�on Samples Reagent kits Extrac�on system Applica�ons Opera�on �me PCR, qPCR, and sequencing FFPE 20 - 30 min 61P series Maelstrom 8 / Maelstrom 48 series / Maelstrom 96 series Key features Only 5 μm thick FFPE sample is enough for use Saving pretreatment �me No toxic solvents throughout the whole extrac�on process Figure 1. The qPCR (A) amplifica�on curves and (B) Ct values of FFPE DNA extracted with TANBead and commercial T kits. Table 1. The comparison of nucleic acids extrac�on perfor- mance between TANBead FFPE kits and Commercial-T FFPE kits in extrac�ng 20 mg pig liver FFPE samples. 11.01±0.52 7.70±0.06 137.65±6.55 91.67±0.76 TANBead Commercial-T 1.91±0.01 2.00±0.01 Yield (μg) A260/280 Extrac�on System Conc. (ng/μL) TANBead Commercial-T 28.78±0.18 31.06±0.88 Mean Ct Extrac�on System (B) (A) RFU 0 -100 0 10 20 30 40 100 200 300 400 500 600 Amplifica�on Cycles Blue: TANBead Red: Commercial-T FFPE DNA Extrac�on
  • 15. Figure 1. Yield comparison of extracted stool DNA using 6SC kit and commercial-Q kit. The extracted stool DNA was subjected to 16S rDNA (indicates bacteria) or GAPDH (indicates human) qPCR analysis, and the Ct values of samples extracted with 6SC kit were lower than that extracted with commer- cial-Q kit. TANBead Stool DNA Kit is designed for rapid, reliable, automated purifica�on of DNA from stool samples. Our magne�c beads-based technology with our corresponding extrac�on system can provide you automated, high-throughput and easy-to-use nucleic acids extrac�on. The extracted nucleic acids can be applied for various applica�ons, such as PCR, qPCR, sequencing (microbiome profiling). Introduc�on Stool DNA Extrac�on TANBead® Stool DNA Kit Specifica�on Samples Reagent kits Extrac�on system Applica�ons Opera�on �me PCR, qPCR, and NGS analysis Stool 40 - 55 min 6SC series Maelstrom 8 / Maelstrom 48 series / Maelstrom 96 series Key features Can acquire both the microbial and the host DNA from stool samples Provides appropriate lysis buffers for either omnivorous or herbivorous species Provide choices for different sample inputs, such as 8, 48, 96 tests per run Q Supplier TANBead SD Mean Mean SD 130.1 Yield (ng/µL) 2.4 11.9 0.4 Cycle threshold TANBead Q 50 20 30 40 10 0 GAPDH Cycle threshold 16S TANBead Q 25 10 15 20 5 0 Table 1. Stool DNA Extrac�on
  • 16. Table 3. Yield measurement and Ct values of extracted DNA from stool samples spiked with Giardia lamblia cyst parasites. Table 2. Yield measurement and 16s qPCR analysis of extracted DNA from stool samples of omnivorous or herbivorous species. TANBead Species Cat Dog Human Yield (μg) 14.71 40.42 32.68 SD 0.06 0.04 0.1 Ct 31.7 32.35 29.53 SD 0.23 0.25 0.17 Cat Dog Rabbit Chinchilla Goat Tortoise Guinea Pig Cow 14.53±1 26.58±0.67 6.4±0.22 18.35±3.8 3.5±1.25 10.15±1.59 37.5±7.60 4.53±0.3 27.37±0.62 17.14±0.36 NA NA 20.22±0.66 28.14±0.71 27.63±0.74 29.99±0.43 5.22±0.51 2.38±0.39 3.25±0.6 4.08±0.46 5.03±1.42 5.2±2.02 33.8±15.64 7.3±1.1 29.32±0.32 Yield (μg) Ct Mean Yield (μg) Ct Mean 19.56±0.28 28.07±0.21 28.65±0.18 20.1±0.51 28.16±0.6 27.62±1.16 29.48±0.46 Species Incuba�on buffer 1: Omnivorous Buffer Incuba�on buffer 2: Herbivorous Buffer Stool DNA Extrac�on
  • 17. Table 1. Yield and quality of extracted DNA from plant samples using 613 kit. Figure 1. Integrity of DNA extracted from plant samples using 613 kit, examined through gel electrophoresis. M Leaves #1 #2 #1 #2 #1 #2 #1 #2 #1 #2 #1 #2 #1 #2 #1 #2 Seeds R i c e S t r a w b e r r y A r a b i d o p s i s E u c a l y p t u s L a u r e l W h e a t T o m a t o C o � o n TANBead Plant DNA Kit is designed for rapid, reliable, automated purifica�on of DNA from plant materials. Our magne�c beads-based technology with our corresponding extrac�on system can provide you automated, high-throughput and easy-to-use nucleic acids extrac�on. The extracted nucleic acids can be applied for various applica�ons, such as PCR, qPCR, and sequencing. Introduc�on Plant DNA Extrac�on TANBead® Plant DNA Kit Specifica�on Samples Reagent kits Extrac�on system Applica�ons Opera�on �me PCR-based genotyping assay and qPCR Leaf , seed or rice grain 613 (45 - 55 min) 613-SE (50 - 60 min) 619 (100 - 120 min) 613 series, 613-SE series, 619 series Maelstrom 8 / Maelstrom 48 series / Maelstrom 96 series Key features Automated magne�c beads-based nucleic acids extrac�on technology High yield and high-quality nucleic acids Provide choices for different sample inputs, such as 8, 48, 96 tests per run 10 kb - 3 kb - 1 kb - Figure 2. Yield, quality and integrity of extracted DNA from rice grain samples using 619 kit. 10 kb - 3 kb - 1 kb - #1 #2 One grain Mean SD Yield (μg) Quality A260/A280 14.1 2.33 3.24 0.33 Sample type Leaves Rice 4.93±0.13 4.79±0.53 3.41±0.04 5.84±0.62 2.5±0.03 2.11±0.22 4.19±0.03 15.05±0.24 1.82±0.02 1.37±0.05 1.89±0.06 1.67±0.11 1.98±0.01 1.88±0.04 1.84±0.01 1.82±0.02 Strawberry Arabidopsis Eucalyptus Laurel Wheat Tomato Co�on Seeds Yield (μg) Quality 260/280 Plant DNA Extrac�on
  • 18. Various leaves DNA are well extracted using the M613-SE kit. Various seeds DNA are well extracted using the M613-SE kit. Table 2. Table 3. Fern Cunninghamia lanceolata Juniper us chinensis L. var. kaizuka Pinaceae Podocarpus macrophyllus Commelina communis L. Bambusoideae Egeria densa Orchidaceae, Orchid Saccharum Areca catechu Oryza sativa, Rice Trachycarpus fortunei, Palm Scheffera arboricola Melon Cabbage Trifolium hybridum Phoebe zhennan Prunus subgen. Cerasus Psidium guajava Aronia melanocarpa Fructus Mori Corymbia citriodora Melaleuca alternifolia Eucalyptus robusta Camellia sinensis Liquidambar formosana Osmanthus fragrans Codiaeum variegatum Acacia confusa Carica papaya Rosa rugosa, Rose leaf Rosa rugosa, Rose petal Passiflora edulis Celosia cristata Corymbia citriodora Laurus nobilis Arabidopsis thaliana Fragaria × ananassa, Strawberry 16.1 9.4 25.2 6.2 11.9 27.1 36.8 19.6 22.3 43.6 27.25 32.03 27.25 17 22.9 3.4 16.7 13.8 28.3 28.4 30 18.4 27.4 36.5 41.5 47.1 12.6 12.5 53.6 41.7 26.4 35.2 8.3 26.3 12.7 18.7 14.3 24.77 37.29 Plant leaves Conc. (ng/µL) Zea mays, Corn Hordeum vulgare, Barley Triticum aestivum Arabidopsis thaliana Sesamum indicum Cucumis sativus, Cucumber Cucurbita pepo, Pumpkin Abelmoschus esculentus Fragaria × ananassa, Strawberry Solanum lycopersicum, Tomato Solanum melongena, Egg Plant Co�on Alstonia scholaris 10.0 10.2 17.4 51.1 8.6 16.0 10.8 14.1 13.3 32.4 17.0 117.1 9.4 Conc. (ng/µL) Plant seeds Plant DNA Extrac�on
  • 19. TANBead Plant RNA Kit is designed for rapid, reliable, automated purifica�on of RNA from the leaves or seeds. Our magne�c beads-based technology with our corresponding extrac�on system can provide you automated, high-throughput and easy-to-use nucleic acids extrac�on. The extracted nucleic acids can be applied for various applica�on, such as RT-PCR. Introduc�on Plant RNA Extrac�on TANBead® Plant RNA Kit Specifica�on Samples Reagent kits Extrac�on system Applica�ons Opera�on �me RT-PCR, qRT-PCR and Northern blot Leaf or seed 45 - 60 min 6K3 series Maelstrom 8 / Maelstrom 48 series / Maelstrom 96 series Key features Automated magne�c beads-based nucleic acids extrac�on technology High yield and high-quality nucleic acids Provide choices for different sample inputs, such as 8, 48, 96 tests per run Table 1. Yield and quality of extracted RNA from plant samples using 6K3 kit. Figure 1. Integrity of RNA extracted from leaves or seeds using 6K3 kit, examined through gel electrophoresis. M Leaves Seeds R i c e S t r a w b e r r y A r a b i d o p s i s E u c a l y p t u s L a u r e l W h e a t T o m a t o C o � o n #1 #2 #1 #2 #1 #2 #1 #2 #1 #2 #1 #2 #1 #2 #1 #2 1.5 kb - 0.5 kb - 0.1 kb - 6.46±0.16 6.46±0.18 6.12±0.24 6.17±0.10 6.18±0.22 6.68±0.20 4.45±0.15 4.9±0.04 Rice Strawberry Arabidopsis Eucalyptus Laurel Wheat Tomato Co�on 1.97±0.01 1.97±0.01 1.95±0 1.94±0.04 1.96±0.01 1.95±0.04 1.72±0.06 2.06±0.18 Yield (μg) 260/280 Sample type Leaves Seeds Plant RNA Extrac�on
  • 20. TANBead Fungi DNA Kit is designed for rapid, reliable, automated purifica�on of DNA from fungi culture. Our magne�c beads-based technology with our corresponding extrac�on system can provide you automated, high-throughput and easy-to-use nucleic acids extrac�on. The extracted nucleic acids can be applied for various applica�ons, such as PCR, qPCR, and sequencing. Introduc�on Fungi DNA Extrac�on TANBead® Fungi DNA Kit Specifica�on Samples Reagent kits Extrac�on system Applica�ons Opera�on �me PCR and qPCR Fungi 50 - 60 min 61F series Maelstrom 8 / Maelstrom 48 series Key features Automated magne�c beads-based nucleic acids extrac�on technology High yield and high-quality nucleic acids Provide choices for different sample inputs, such as 8, 48 tests per run Table 1. Yield and quality of extracted DNA from yeast using 61F kit. 1 OD 2 OD Mean 0.81 1.95 SD 0.02 0.39 0.021 Mean Yield (μg) 1.96 Quality A260/A280 SD 0.035 0.01 Figure 1. Integrity of DNA extracted from yeast samples using 61F kit, examined through gel electrophoresis. 1 OD 2 OD #1 #2 #3 #1 #2 #3 3 kb - 10 kb - 1 kb - M Figure 2. PCR amplifica�on of the tandemly repe��ve subele- ments (TRS)-1 and TRS-2 from five isolates of T. rubrum.(Chien-yio Lin, 2018) Reference. Chien-yio Lin, Hsiu-Jung Lo, Ming-Gene Tu et al. The survey of �nea capi�s and scalp dermatophyte carriage in nursing home residents. Medical Mycology. 2018; 56:180-185. M 1 2 3 4 5 M M 1 2 3 4 5 M 800 - 500 - (bp) - 800 - 500 (bp) TRS-1 TRS-2 1: scalp 2: scalp 3: scalp 4: right sole 5: right big toe Fungi DNA Extrac�on
  • 21. Food and Feed DNA Extrac�on TANBead® Nucleic Acid Extrac�on Kit (6GM) is designed for a simple and convenient method of high-quality DNA isolated from raw material and recover the highly fragmented DNA from processed food, which can be down to 100 bp. With TANBead® automated extractors, this kit can simplify the nucleic acid extrac�on process, abstaining from manual processing. The �me needed is reduced, repe��ve centrifuga�on is removed and risk of cross-contamina�on is minimized. The extracted DNA is ready-to-use for detec�on of gene�cally modified organisms (GMO) and food adultera�on. Introduc�on TANBead® Food and Feed DNA Kit Specifica�on Samples Reagent kits Extrac�on system Applica�ons Opera�on �me PCR-based assays Food material or products, Feed 45 - 55 min 6GM series Maelstrom 8 / Maelstrom 48 series Maelstrom 96 series Key features Automated magne�c beads-based nucleic acids extrac�on technology and prefilled reagent system Can recover short DNA fragments (down to 100 bp) in processed food Provide choices for different sample inputs, such as 8, 48, 96 tests per run Figure 1. The performance of Food and Feed DNA Auto Kit was examined through qPCR analysis. The results indicated that the extracted DNA can be used for GMO detec�on (using Thermo GMO Screening kit). GMO (50 mg) was extracted, then the P35 and TNOS genes were detected with qPCR along with PC (posi�ve control), IC (internal control) and NC (nega�ve control). Table 1. The DNA extracted from 50 mg sample was examined with Qubit™ dsDNA HS assay kit. Corn Soybean Chip Cereal Tofu Miso Dog food Pig food 20-40 ng / µL 25-50 ng / µL 2-10 ng / µL 0-10 ng / µL 10-30 ng / µL 5-20 ng / µL 10-35 ng / µL 10-50 ng / µL Expected yield Product type Sample : GMO soy Target : P35S, TNOS P35S (Sample) P35S (PC) TNOS (PC) TNOS (Sample) NC Amplifica�on Cycles RFU 10 30 50 0 20 40 0 2000 4000 1000 3000 Sample : GMO soy Target : IC (plant) Amplifica�on Cycles RFU 10 30 50 0 0 1000 2000 3000 500 1500 2500 20 40 NC IC Food and Feed DNA Extrac�on
  • 22. Forensic DNA Extrac�on TANBead® Nucleic Acid Extrac�on Kit (6TF) is dedicated to isolate DNA from small sample quan�ty or challenging sizes. Samples need to be treated by following the prepara�on steps, incubated in incuba�on buffer, and treated with Proteinase K and DTT (Dithiothreitol). The lysates need to be added into Auto Plate or Auto Tube and automa�cally processed using TANBead® Nucleic Acid Extractor. The highly extracted and purified DNA can be directly applied for various applica�ons, including real-�me PCR, short tandem repeat (STR) analysis, mitochondrial DNA (mtDNA) analysis and other forensic tests. Introduc�on TANBead® Forensic DNA Kit Specifica�on Samples Reagent kits Extrac�on system Applica�ons Opera�on �me PCR and qPCR cigare�e, hair, blood stain, dried blood spot, semen stain, chewing gun, nail 55 - 75 min 6TF series Maelstrom 8 / Maelstrom 48 series Maelstrom 96 series Key features Automated magne�c beads-based nucleic acids extrac�on technology Purify limited nucleic acids from various forensic sample types Provide choices for different sample inputs, such as 8, 48, 96 tests per rum Figure 1. The qPCR results of GAPDH and ACTIN (ACTB). The nucleic acid was extracted from various samples using TANBead® Forensic DNA Extrac�on Kit. Each sample type was replicated three �mes. Figure 2. Agarose gel electrophoresis of nucleic acids extracted from seven different sample types with TANBead® Forensic DNA Extrac�on Kit. Mitochondrial DNA was amplified with PCR (Product size: 1200 bp) and 50 μL of each sample was loaded on a 1% agarose gel. Marker: Bio-1kbTM Mass DNA Ladder; NC: Nega�ve control; Lane 1: Blood stain; Lane 2: Dried blood spot; Lane 3: Semen stain; Lane 4: Nail; Lane 5: Hair follicles; Lane 6: Chewing gum; Lane 7: Cigare�e bu�s. Extrac�on on Maelstrom 4800 1000 bp - Extrac�on on Maelstrom 4800 N a i l 0 10 20 30 40 Ct Value of GAPDH B l o o d S t a i n S e m e n S t a i n H a i r F o l l i c l e s C h e w i n g C u m C i g a r e � e B u � s D r i e d B l o o d S p o t Extrac�on on Maelstrom 4800 N a i l 0 10 20 30 40 Ct Value of ACTB B l o o d S t a i n S e m e n S t a i n H a i r F o l l i c l e s C h e w i n g C u m C i g a r e � e B u � s D r i e d B l o o d S p o t Forensic DNA Extrac�on
  • 23. TANBead® RT-LAMP SARS-CoV-2 detec�on kit is designed for rapid detec�on of purified nucleic acids containing SARS-CoV-2. The purified nucleic acids can be obtained from swabs, saliva, serum, and urine specimens using TANBead® Nucleic acids extrac�on kits (665 and 685 series, page 16~18). Results are monitored by fluorescent signal and indicates SARS-CoV-2 RNA presence. This assay is based on the Loop-mediated Isothermal Amplifica�on (LAMP) reac�on, which is one of the Nucleic Acid Amplifica�on Tests (NAATs). The reac�on u�lizes a set of primers to rapidly amplify the specific DNA fragment at 65°C using reverse transcriptase and recombinant Bst DNA polymerase. This assay is very �me-efficient and sensi�ve, which can give results within 30 minutes, and strong posi�ve samples can be observed as soon as 10 minutes. Introduc�on RT-LAMP Detec�on TANBead® RT-LAMP Kit Specifica�on Reagent kits Applica�ons Time-to-result SARS-CoV-2 detec�on 30 minutes Samples Purified nucleic acid System Real-�me PCR instruments TANBead® RT-LAMP kit Key features Highly sensi�ve nucleic acid detec�on Isothermal amplifica�on Simple and low-cost equipment Rapid process typically less than 30 minutes Figure 1. Comparison of RT-LAMP and RT-qPCR for the detec�on of serial-diluted standard SARS-CoV-2 RNA samples. A 10-fold serial dilu�on of SARS-CoV-2 RNA star�ng from 107 to 10 copies per reac�on was added into TANBead® RT-LAMP reagents or D-brand detec�on kit. SARS-CoV-2 detec�on with (A) TANBead® RT-LAMP at 65°C or (B) D-brand detec�on kit was performed in CFX96™ Real-Time PCR Detec�on System. The rela�ve fluores- cence units (RFU) indicated the presence of SARS-CoV-2 N gene nucleic acid in samples. The Ct values of each dilutant detected with D-brand kit, star�ng from 107 to 10 copies and nega�ve control, were 15.06, 18.61, 21.85, 25.06, 28.46, 21.18, 36.97 and ND, respec�vely. Meanwhile, Ct values of dilutants detected with TANBead® RT-LAMP kit were much more compe��ve. Figure 2. Detec�on of SARS-CoV-2 RNA via real-�me RT-LAMP. Time-to-results were demonstrated using low, medium, and high RNA input (each n = 4), where the amount of RNA from each clinical sample was pre-determined with RT-qPCR. Regardless of the input amount, TANBead® RT-LAMP amplified SARS-CoV-2 RNA in less than 20 minutes. No template control (NTC) signal was not detected even a�er 40 minutes of assay. TANBead L brand High RNA input Medium RNA input Low RNA input NTC Time to result (min) 20 30 40 10 0 RFU 0 5 10 25 30 20 15 0 1000 2000 3000 4000 5000 6000 7000 8000 9000 Amplifica�on curve of RT-LAMP Time(min) RFU Amplifica�on curve of RT-qPCR Cycles 0 500 1000 1500 2000 0 40 30 10 20 (A) (B) RT-LAMP Detec�on
  • 24. mitochondrial DNA gDNA Sample Blood stain Chewing gum Cigare�e bu�s Sperm Stamps, Envelopes Fingernails Hair, Hair roots B lo o d D N A ( 6 1 1 ) B l o o d R N A ( 6 2 1 ) F F P E D N A ( 6 1 P ) H P V D N A ( 6 1 H ) P la n t D N A ( 6 1 3 ) P la n t R N A ( 6 K 3 ) T i s s u e D N A ( 6 T 2 ) T is s u e R N A ( 6 K 2 ) S t o o l D N A ( 6 S C ) F u n g i ( 6 1 F ) S T I ( 6 S T ) H B V ( 6 1 5 ) O p � P u r e B lo o d D N A ( 6 1 E ) O p � P u r e c f D N A ( 6 1 C ) D r ie d B lo o d S p o t ( 6 1 E - B S ) O p � P u r e V ir a l ( 6 6 5 ) V ir a p id V i r a l ( 6 8 5 ) P la n t D N A ( 6 1 3 - S E ) B a c t e r ia D N A ( 6 1 G ) B a c t e r ia D N A ( 6 1 3 - S E ) Sample Target gDNA Total RNA Bacteria Parasite Virus gDNA Total RNA gDNA Total RNA gDNA Bacteria Virus cfDNA gDNA Bacteria Virus gDNA Bacteria Virus Bacteria Virus Bacteria Bacteria Virus gDNA Total RNA Bacteria Bacteria Virus gDNA Bacteria Virus gDNA Total RNA Bacteria Virus gDNA Total RNA gDNA gDNA Bacteria Virus gDNA Bacteria Virus gDNA Bacteria Virus gDNA Total RNA gDNA gDNA Total RNA Bacteria Virus Blood Buffy coat Plasma / Serum Bone marrow Body fluids BAL, aspirates Bacterial culture Cultured cells Sputum Saliva Solid �ssue Rodent tails FFPE Swab Urine Stool Fish Yeast Plant: seeds, leaves Water Cell culture supernatant Liquid sample transport media Leukocyte Clo�ed, Dried blood 2 4 1 1 3 Note. 1. Wang L, Yang S, Yan X, et al. Comparing the yield of oropharyngeal swabs and sputum for detec�on of 11 common pathogens in hospitalized children with lower respiratory tract infec�on. Virol J. 2019 Jun 24:16(1):84. 2. Yu RL, Tu SC, Wu RM, et al. Interac�ons of COMT and ALDH2 Gene�c Polymorphisms on Symptoms of Parkinson’s Disease. Brain Sci. 2021 Mar 12;11(3):361. 3. Hsu JC, Huang, H, Lin H, et al. Applying Modified VP53A Recombinant Protein as an An�-White Spot Syndrome Virus Biological Agent in Litopenaeus vannamei Farming. Viruses 2022 Jun 21;14:1353. 4. Tsai K, Tu Y, Wu C, et al. First detec�on and phylogene�c analysis of lumpy skin disease virus from Kinmen Island, Taiwan in 2020. The Journal of Veterinary Medical Science 2022 Jun 13;21-0649. Kit Reference TANBead Forensic DNA Recommended: Internally Validated kit with proven extrac�on efficiency on the sample type and target. Compa�ble: Can be used on the sample type and target. Not internally validated - Extrac�on efficiency and results may differ. User-valida�on sample type and target. Kit Reference
  • 25. Reagent Kits for : Maelstrom 8 Autostage, Maelstrom 4800 and Maelstrom 4810 301126 301127 301128 301307 301129 301400 301401 301134 301371 301135 301372 301383 301384 301385 301389 301152 301153 301148 301149 301346 301572 301573 301431 301432 301130 301131 301132 301306 301133 301260 301366 301367 301150 301151 301424 301425 301257 301138 301294 301139 301295 M611A46 M611S46 M61EA46 M61EA10 M61ES46 M621A46 M621S46 M613A46* M613A46-SE* M613S46* M613S46-SE* M6K3A46* M6K3S46* M61CA46 M61CS46 M61PA46 M61PS46 M665A46 M665S46 M665A10 M685A46 M685S46 M61HA46 M61HS46 M612A46 M612S46 M6T2A46 M6T2A10 M6T2S46 M6T2046 M6K2A46 M6K2S46 M61FA46 M61FS46 M6TFA46 M6TFS46 M61G046 M61GA46 M61GA46-SE M61GS46 M61GS46-SE TANBead Blood DNA Auto Plate TANBead Blood DNA Auto Tube TANBead Op�Pure Blood DNA Auto Plate TANBead Op�Pure Blood DNA Bulk Plate TANBead Op�Pure Blood DNA Auto Tube TANBead Blood RNA Auto Plate TANBead Blood RNA Auto Tube TANBead Plant RNA Auto Plate TANBead Plant RNA Auto Tube TANBead Op�Pure cfDNA Auto Plate TANBead Op�Pure cfDNA Auto Tube TANBead Op�Pure FFPE DNA Auto Plate TANBead Op�Pure FFPE DNA Auto Tube TANBead Op�Pure Viral Auto Plate TANBead Op�Pure Viral Auto Tube TANBead Op�Pure Viral Bulk Plate TANBead Virapid Virus Auto Plate TANBead Virapid Virus Auto Tube TANBead HPV Auto Plate TANBead HPV Auto Tube TANBead Tissue DNA Auto Plate TANBead Tissue DNA Auto Tube TANBead Tissue Total DNA Auto Plate TANBead Tissue Total DNA Bulk Plate TANBead Tissue Total DNA Auto Tube TANBead Tissue Total DNA Auto Kit TANBead Tissue RNA Auto Plate TANBead Tissue RNA Auto Tube TANBead Fungi DNA Auto Plate TANBead Fungi DNA Auto Tube TANBead Forensic DNA Auto Plate TANBead Forensic DNA Auto Tube TANBead Gram Bacteria DNA Auto Kit TANBead Gram Bacteria DNA Auto Plate TANBead Gram Bacteria DNA Auto Tube 96 96 96 960 96 96 96 96 96 96 96 96 96 96 96 96 96 96 96 960 96 96 96 96 96 96 96 960 96 96 96 96 96 96 96 96 96 96 96 96 96 Sample Descrip�on Test Reference No. Ordering No. Blood Plant cfDNA FFPE HPV Virus Tissue Bacteria Fungi Forensic Plasmid TANBead Plant DNA Auto Plate TANBead Plant DNA Auto Tube 301578 301579 M6PEA46* M6PES46* TANBead Plasmid Extrac�on Auto Plate TANBead Plasmid Extrac�on Auto Tube 96 96 Note: black font = IVD and RUO available blue font = RUO available Ordering Informa�on
  • 26. Reagent Kits for : Maelstrom 8 Autostage, Maelstrom 4800 and Maelstrom 4810 Reagent Kits for : Maelstrom 9600 and Maelstrom 9610 301186 301187 301188 301189 301402 301403 301194 301379 301259 301378 301406 301407 301377 301386 301212 301213 301224 301345 301209 301574 301575 301591 301592 301190 301191 301192 301193 301404 301405 W611A46 W611S66 W61EA46 W61ES66 W621A46 W621S66 W613A46* W613A46-SE* W613S66* W613S66-SE* W6K3A46* W6K3S66* W61CA46 W61CS66 W61PA46 W61PS66 W665A46 W665A10 W665S66 W685A46 W685S66 W61HA46 W61HS66 W612A46 W612S66 W6T2A46 W6T2S66 W6K2A46 W6K2S66 TANBead Blood DNA Auto Plate TANBead Blood DNA Auto Tube TANBead Op�Pure Blood DNA Auto Plate TANBead Op�Pure Blood DNA Auto Tube TANBead Blood RNA Auto Plate TANBead Blood RNA Auto Tube TANBead Plant RNA Auto Plate TANBead Plant RNA Auto Tube TANBead Op�Pure cfDNA Auto Plate TANBead Op�Pure cfDNA Auto Tube TANBead Op�Pure FFPE DNA Auto Plate TANBead Op�Pure FFPE DNA Auto Tube TANBead Op�Pure Viral Auto Plate TANBead Op�Pure Viral Bulk Plate TANBead Op�Pure Viral Auto Tube TANBead Virapid Virus Auto Plate TANBead Virapid Virus Auto Tube TANBead HPV DNA Auto Plate TANBead HPV DNA Auto Tube TANBead Tissue DNA Auto Plate TANBead Tissue DNA Auto Tube TANBead Tissue Total DNA Auto Plate TANBead Tissue Total DNA Auto Tube TANBead Tissue RNA Auto Plate TANBead Tissue RNA Auto Tube 96 72 96 72 96 72 96 96 72 72 96 72 96 72 96 72 96 960 72 96 72 96 72 96 72 96 72 96 72 Sample Descrip�on Test Reference No. Ordering No. Blood Plant cfDNA FFPE Virus Tissue 301635 301636 301387 301388 301414 301415 M6GMA46* M6GMS46* M6SCA46 M6SCS46 M6STA46 M6STS46 TANBead Food and Feed DNA Auto Plate TANBead Food and Feed DNA Auto Tube TANBead Stool Cell DNA Auto Plate TANBead Stool Cell DNA Auto Tube TANBead STIs Extrac�on Auto Plate TANBead STIs Extrac�on Auto Tube 96 96 96 96 96 96 Sample Descrip�on Test Reference No. Ordering No. Food and Feed Stool STIs Note: black font = IVD and RUO available blue font = RUO available Tissue TANBead Plant DNA Auto Plate TANBead Plant DNA Auto Tube Note: black font = IVD and RUO available blue font = RUO available HPV Ordering Informa�on
  • 27. Reagent Kits for : Maelstrom 9600 and Maelstrom 9610 Reagent Kits for : Maelstrom 2400 and Maelstrom 2410 301411 301631 L91C045 L91E045* TANBead Op�Pure cfDNA Auto Kit TANBead Blood DNA Auto Kit 48 48 cfDNA Blood Sample Descrip�on Test Reference No. Ordering No. Note: black font = IVD and RUO available blue font = RUO available Sample Descrip�on Test Reference No. Note: black font = IVD and RUO available blue font = RUO available Ordering No. W6TFA46 W6TFS66 W61GA46 W61GS66 W6PEA46* W6PES66* W6GMA46* W6GMS66* W6SCA46 W6SCS66 W6STA46 W6STS66 TANBead Forensic DNA Auto Plate TANBead Forensic DNA Auto Tube TANBead Gram Bacteria DNA Auto Plate TANBead Gram Bacteria DNA Auto Tube TANBead Plasmid Extrac�on Auto Plate TANBead Plasmid Extrac�on Auto Tube TANBead Food and Feed DNA Auto Plate TANBead Food and Feed DNA Auto Tube TANBead Stool Cell DNA Auto Plate TANBead Stool Cell DNA Auto Tube TANBead STIs DNA Auto Plate TANBead STIs DNA Auto Tube 96 72 96 72 96 72 96 72 96 72 96 72 Bacteria Plasmid Food and Feed Stool STIs 301291 301426 301198 301199 301580 301581 301637 301638 301392 301391 301595 301596 Forensic Ordering Informa�on