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Quest Journals
Journal of Medical and Dental Science Research
Volume 4~ Issue 3 (2017) pp: 14-17
ISSN(Online) : 2394-076X ISSN (Print):2394-0751
www.questjournals.org
*Corresponding Author: Dr. Pradnya V. Bansode1
14 | Page
Professor And Head Of Department, Department Of Conservative Dentistry & Endodontics,
Govt. Dental College And Hospital, Aurangabad-431001, (M.S), College, MUHS, India.
Research Paper
Dense Evaginatus: Management Using Novel Materials
A Case Report
Dr. Pradnya V. Bansode1
, Dr. M.B. Wavdhane3
, Dr. Seema D. Pathak3
,
Dr.Shirish B. Khedgikar4
, *Dr. Hardik B. Rana5
1Professor And Head Of Department, Department Of Conservative Dentistry & Endodontics, Govt. Dental
College And Hospital, Aurangabad-431001, (M.S), College, MUHS, India. Mobile No.:- 09421679094
2Associate Professor, Department Of Conservative Dentistry & Endodontics, Govt. Dental College And
Hospital, Aurangabad-431001, (M.S), College, MUHS, India. Mobile No.:- 09890053082
3Associate Professor, Department Of Conservative Dentistry & Endodontics, Govt. Dental College And
Hospital, Aurangabad-431001, (M.S), College, MUHS, India. Mobile No.:- 09850694750
4Assistant Professor, Department Of Conservative Dentistry & Endodontics, Govt. Dental College And
Hospital, Aurangabad-431001, (M.S), College, MUHS, India. Mobile No.:- 09850055455
5PG Student, Department Of Conservative Dentistry & Endodontics, Govt. Dental College And Hospital,
Aurangabad (M.S), College, MUHS, India.
Received 15 Apr, 2017; Accepted 28 Apr, 2017 © The author(s) 2017. Published with open access at
www.questjournals.org
ABSTRACT: Dens evaginatus is an uncommon developmental anomaly of human dentition characterized by a
projection of enamel and dentin that usually encloses pulp tissue. Most commonly found as the tubercle on the
occlusal surface of mandibular premolars and lingual surface of anterior teeth.Due to occlusal trauma this
tubercle tends to fracture thus exposing the pathway to the pulp chamber of teeth. This case reports about the
presentation of dens evaginatus in mandibular premolar 35 which was associated with open apex and chronic
apical periodontitis. Root canal treatment was performed with tooth 35. DFDBA apical barrier and Biodentine
as an apical plug was placed showing successful management of the same.
Keywords: Biodentine,Dens evaginatus, DFDBA, Open apex, Talon cusp, Tubercle
I. INTRODUCTION
Dens evaginatus (DE) or evaginatedodontoma is a developmental anomaly characterized by the
presence of an accessory cusp, abnormal tubercle, or elevation that occurs in human dentition. It consists of
enamel covering a dentinal core that usually contains pulp tissue.The presence of pulp within the cusp-like
tubercle has clinical significance and distinguishes it fromsupplemental cusps, such as cusp of carabelli [1].
Early detection and management of this condition are important because trauma during mastication causes
fracture or wear of the tubercle that leads to necrosis of pulp and periapical infection. This condition is
predominantly found on the occlusal surface ofmandibular premolars [2] and lingual surface of anterior teeth
(mainly maxillary lateral incisors) [3]. Prevalence of DE studied in several population ranges from 1% to 4%
[4]; the prevalence was found in approximately 2% of Asian descent population [5]. Higher rates of occurrence
were reported among the Chinese population, 1.29%–3.6% [6]. It is usually observed as bilateral, symmetric
distribution, with a slight sexual predilection for females.
II. CASE REPORT
A 30-year-old male visited the department with concerned about the pain and difficulty on chewing on
his left side of jaw. On examination patient had fair oral hygiene and occlusal tubercle present in mandibular
second premolar was found (Figure 1). Tooth# 35 was tender on percussion. Pulp vitality testing with electric
pulp tester showed no response with the same. Intraoral Periapical Radiograph (IOPAR) revealed periapical
radiolucency with open apex in relation to 35 (Figure 2). No systematic and congenital disease was seen.As the
root was having an open apex, it was planned to start with conventional root canal therapy followed by the
placement of an apical barrier and apical plug and back filling with guttapercha.Access cavity preparation was
done on tooth 35. By keeping in mind that not to weaken the radicular dentin more, minimal instrumentation
Dense Evaginatus: Management Using Novel Materials- A Case Report
*Corresponding Author: Dr. Pradnya V. Bansode1
15 | Page
was done by circumferential filling using hand K-files (Mani, Inc., Japan).During instrumentation, the canals
were irrigated copiously with 2.5% sodium hypochlorite solution followed by final wash with normal saline to
remove extruded hypochlorite through open apex if any. Drying of the canal was done with absorbent paper
points (Dentsply, Maillefer). Triple antibiotic paste root canal dressing was performed and changed every 14
days followed by frequent root canal irrigation with hypochlorite and saline for the period of one month. The
access cavity was temporized with the intermediate restorative material (IRM, Dentsply Caulk, Milford, USA).
After one month patient was asymptomatic.
Figure 1: Occlusal tubercle in mandibular pre molars.Figure 2: Diagnostic IOPAR, periapical radiolucency
and open apex with respect to 35.
The tooth was again isolated under rubber dam and the canals thoroughly irrigated with hypochlorite
andsaline to wash out any remnants of triple antibiotic paste. DFDBA was mixed with normal saline to packable
consistency. It was then carried to periapical area under radiographic guidance with the help of
endodonticplugger having rubber stop at pre adjusted working length (fig 3). After having confirmed an
adequate matrix mass of DFDBA, Biodentine was mixed and placed over DFDBA with the help of messing gun
to getcompact plug of 3-4 mm (fig 4). Biodentine plug was allowed to set for 10-12 minutes after which
reminder of canalwas obturated with guttapercha. Access opening was sealed with high copper silver
amalgam.(fig 5).At 6 months follow up healing in periapical area can be appreciated (fig 6).
Figure 3: DFDBA placement using pluggerFigure 4: Biodentine as an apical plug
Figure 5: Post-operative radiographFigure 6: 6 months follow up
Dense Evaginatus: Management Using Novel Materials- A Case Report
*Corresponding Author: Dr. Pradnya V. Bansode1
16 | Page
III. DISCUSSION
Dens evaginatus is a tooth anomaly with considerable clinical significance causing occlusal
interference.Maintaining cleanarea between the nodule and the tooth becomes difficult, and cariesis often found.
There are high chances of pulp exposureduring early phases of root development, resulting in pulpnecrosis and
incomplete root formation.The unusual tubercleor elevation on the tooth surface is the usual presentation
butsometimes, due to fracture or attrition, no external evidenceof the malformation may be evident [7].Thus,
early detection ofthese conditions is so important that preventivemanagementcan be started as early as possible.
In the tooth/teeth with DEwith vital pulp, selective reduction of the opposing occludingteeth can be done or, in a
situation where the tubercle hasfractured, it can be sealed with resin. In the case of DE withpulp exposure during
the early phase of root development,pulpotomy is suggested. Ifthe pulp is necrotic, root end barrier in the case of
theimmature apex and conventional root canal treatment shouldbe performed on the mature tooth [1].
Interappointment antimicrobialmedication can be advantageous to curtail bacterial regrowthand possibly even
improve bacterial suppression [8]. Studiesreported the clinical effectiveness of triple antibiotic paste incases of
apical periodontitis [9,10].The antibiotic combinationwas reported to bemore effective againstmixed
bacterialflora as in infected root canal tested when compared withcalcium hydroxide, iodine potassium iodide,
or iodoform[11]. Gomes-Filho et al. [12] evaluated the response andconcluded that triple antibiotic paste is a
biocompatibleintracanal medicament.
A study conducted byWindley et al.[9] observed that the use of triple antibiotic paste
followingirrigation resulted in significant reduction in bacteria comparedto that of irrigation with sodium
hypochlorite alone.Studies have demonstrated that predictable disinfection ofthe root canal systemcanbe
achievedbyproper antimicrobialintracanal medication [13]. The major problem in cases of a wide open apex is
the need to limit the material to the perforation, thus avoiding theextrusion of a large amount of material into the
periodontal tissue [14,15]. A large volume of the extrudedmaterial may set before it disintegrates and get
resorbed. This might result in the persistence of the inflammatory process, which may complicate or even
prevent repair of the tissue [14,15] Using a matrix avoidsthe extrusion of the material into the periodontal
tissues, reduces leakage in the sealing material and allowsfavorable response of the periodontal tissues.DFDBA
is obtained from cortical bone of long bones due to its high content of bone inductive proteins and less antigenic
activity than cancellous bone. Thecollagen matrix present in DFDBA acts as a scaffold that provides
osteoconductive properties alone side theosteoinductive behavior [16]. BMPs are associated with the organic
matrix of bone and embedded withinmineral content, so demineralised process in formation of DFDBA
increases its bioavailability. BMPs attractmesenchymal stem cells and induce them to differentiate into
chondrocytes leading into endochondral boneformation [17]. This property is helpful in healing of large
periapical lesions. Taking into consideration all these advantages of DFDBA, we have used these materials as an
apical barrier matrix over which Biodentine was placed proving success of this case.
IV. CONCLUSION
The communication of root canal to oral cavity due to the tubercle openingmay contributes to
harboring virulent microorganismsthat succeeds in colonizing the canal and thus periapex. The clinician should
be aware of such anomaliesand their consequences so that proper treatment modalitiescan be instituted. Early
detection and careful treatment planning areneeded to prevent further complication of the condition.
REFERENCES
[1]. M. E. Levitan and V. T. Himel, “Dens evaginatus: literaturereview, pathophysiology, and comprehensive treatment
regimen,”Journal of Endodontics, vol. 32, no. 1, pp. 1–9, 2006.
[2]. S. Stecker and A. J. DiAngelis, “Dens evaginatus. A diagnosticand treatment challenge,”The Journal of the American
DentalAssociation, vol. 133, no. 2, pp. 190–193, 2002.
[3]. E. Dankner, D. Harari, and I. Rotstein, “Dens evaginatus ofanterior teeth. Literature review and radiographic survey of15,000
teeth,” Oral Surgery, Oral Medicine, Oral Pathology, OralRadiology, and Endodontics, vol. 81, no. 4, pp. 472–475, 1996.
[4]. F. J.HillandW. J. Bellis, “Dens evaginatus and itsmanagement,”British Dental Journal, vol. 156, no. 11, pp. 400–402, 1984.
[5]. C. E. Jerome and R. J. Hanlon Jr., “Dental anatomical anomaliesin Asians and Pacific Islanders.,” Journal of the California
DentalAssociation, vol. 35, no. 9, pp. 631–636, 2007.
[6]. G. S. Kocsis, A. Marcsik, E. L. K´okai, and K. S. Kocsis,“Supernumerary occlusal cusps on permanent human
teeth,”ActaBiologicaSzegediensis, vol. 46, no. 1-2, pp. 71–82, 2002.
[7]. A. Ayer, M. Vikram, and P. Suwal. “Dens Evaginatus: A Problem-Based ApproachCase Reports in Dentistry” Volume 2015,
Article ID 393209
[8]. Z. Metzger, B. Basrani, and H. E. Goodis, “Instruments, materials,and devices,” in Cohen’s Pathways of the Pulp, K.
M.Hargreaves and S. Cohen, Eds., pp. 223–282, Elsevier, Mosby,2011.
[9]. [9] W. Windley III, F. Teixeira, L. Levin, A. Sigurdsson, and M.Trope, “Disinfection of immature teeth with a triple
antibioticpaste,” Journal of Endodontics, vol. 31, no. 6, pp. 439–443, 2005.
[10]. B. Thibodeau, F. Teixeira, M. Yamauchi, D. J. Caplan, and M.Trope, “Pulp revascularization of immature dog teeth withapical
periodontitis,” Journal of Endodontics, vol. 33, no. 6, pp.680–689, 2007.
[11]. R. C. Pallotta, M. S. Ribeiro, andM. E. D. L.Machado, “Determinationof the minimum inhibitory concentration of
fourmedicaments used as intracanal medication,” Australian EndodonticJournal, vol. 33, no. 3, pp. 107–111, 2007.
Dense Evaginatus: Management Using Novel Materials- A Case Report
*Corresponding Author: Dr. Pradnya V. Bansode1
17 | Page
[12]. J. E. Gomes-Filho,P.C.T.Duarte, C. B. deOliveira et al., “Tissuereaction to a triantibiotic paste used for endodontic tissue
selfregenerationof nonvital immature permanent teeth,” Journal ofEndodontics, vol. 38, no. 1, pp. 91–94, 2012.
[13]. C. J. Baumgartner, J. F. Siqueira, C. M. Sedgley, and A. Kishen,“Microbiology of endodontic disease,” in Ingle’s Endodontics 6,
J.I. Ingle, L. K. Bakland, and J. C. Baumgartner, Eds., pp. 221–308,BC Decker Inc, Hamilton, Canada, 2008.
[14]. Broon NJ, Bramante CM, Assis GF, Bortoluzzi EA, Bernardineli N, Moraes IG, et Healing of root perforations treated
withmineral trioxide aggregate (MTA) and Portland cement. J Appl Oral Sci 2006;14:305-11.
[15]. Holland R, Mazuqueli L, Souza V, Murata SS, Dezan E Jr. Influence of the type of vehicle and limit of obturation on apical
andperiapical tissue response in dogs teeth after root canal filling with mineral trioxide aggregate. J Endod 2007;33:693-7.
[16]. Drosos GI, Kazakos KI, Kouzoumpasis P &Verettas DA.(2007). Injury;38Suppl 4:S13-21.
[17]. Esposito M, Piattelli M, Pistilli R, Pellegrino G. &Felice P. Eur J Oral Implantol 2010;3(4):297-305.

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Dense Evaginatus: Management Using Novel Materials A Case Report

  • 1. Quest Journals Journal of Medical and Dental Science Research Volume 4~ Issue 3 (2017) pp: 14-17 ISSN(Online) : 2394-076X ISSN (Print):2394-0751 www.questjournals.org *Corresponding Author: Dr. Pradnya V. Bansode1 14 | Page Professor And Head Of Department, Department Of Conservative Dentistry & Endodontics, Govt. Dental College And Hospital, Aurangabad-431001, (M.S), College, MUHS, India. Research Paper Dense Evaginatus: Management Using Novel Materials A Case Report Dr. Pradnya V. Bansode1 , Dr. M.B. Wavdhane3 , Dr. Seema D. Pathak3 , Dr.Shirish B. Khedgikar4 , *Dr. Hardik B. Rana5 1Professor And Head Of Department, Department Of Conservative Dentistry & Endodontics, Govt. Dental College And Hospital, Aurangabad-431001, (M.S), College, MUHS, India. Mobile No.:- 09421679094 2Associate Professor, Department Of Conservative Dentistry & Endodontics, Govt. Dental College And Hospital, Aurangabad-431001, (M.S), College, MUHS, India. Mobile No.:- 09890053082 3Associate Professor, Department Of Conservative Dentistry & Endodontics, Govt. Dental College And Hospital, Aurangabad-431001, (M.S), College, MUHS, India. Mobile No.:- 09850694750 4Assistant Professor, Department Of Conservative Dentistry & Endodontics, Govt. Dental College And Hospital, Aurangabad-431001, (M.S), College, MUHS, India. Mobile No.:- 09850055455 5PG Student, Department Of Conservative Dentistry & Endodontics, Govt. Dental College And Hospital, Aurangabad (M.S), College, MUHS, India. Received 15 Apr, 2017; Accepted 28 Apr, 2017 © The author(s) 2017. Published with open access at www.questjournals.org ABSTRACT: Dens evaginatus is an uncommon developmental anomaly of human dentition characterized by a projection of enamel and dentin that usually encloses pulp tissue. Most commonly found as the tubercle on the occlusal surface of mandibular premolars and lingual surface of anterior teeth.Due to occlusal trauma this tubercle tends to fracture thus exposing the pathway to the pulp chamber of teeth. This case reports about the presentation of dens evaginatus in mandibular premolar 35 which was associated with open apex and chronic apical periodontitis. Root canal treatment was performed with tooth 35. DFDBA apical barrier and Biodentine as an apical plug was placed showing successful management of the same. Keywords: Biodentine,Dens evaginatus, DFDBA, Open apex, Talon cusp, Tubercle I. INTRODUCTION Dens evaginatus (DE) or evaginatedodontoma is a developmental anomaly characterized by the presence of an accessory cusp, abnormal tubercle, or elevation that occurs in human dentition. It consists of enamel covering a dentinal core that usually contains pulp tissue.The presence of pulp within the cusp-like tubercle has clinical significance and distinguishes it fromsupplemental cusps, such as cusp of carabelli [1]. Early detection and management of this condition are important because trauma during mastication causes fracture or wear of the tubercle that leads to necrosis of pulp and periapical infection. This condition is predominantly found on the occlusal surface ofmandibular premolars [2] and lingual surface of anterior teeth (mainly maxillary lateral incisors) [3]. Prevalence of DE studied in several population ranges from 1% to 4% [4]; the prevalence was found in approximately 2% of Asian descent population [5]. Higher rates of occurrence were reported among the Chinese population, 1.29%–3.6% [6]. It is usually observed as bilateral, symmetric distribution, with a slight sexual predilection for females. II. CASE REPORT A 30-year-old male visited the department with concerned about the pain and difficulty on chewing on his left side of jaw. On examination patient had fair oral hygiene and occlusal tubercle present in mandibular second premolar was found (Figure 1). Tooth# 35 was tender on percussion. Pulp vitality testing with electric pulp tester showed no response with the same. Intraoral Periapical Radiograph (IOPAR) revealed periapical radiolucency with open apex in relation to 35 (Figure 2). No systematic and congenital disease was seen.As the root was having an open apex, it was planned to start with conventional root canal therapy followed by the placement of an apical barrier and apical plug and back filling with guttapercha.Access cavity preparation was done on tooth 35. By keeping in mind that not to weaken the radicular dentin more, minimal instrumentation
  • 2. Dense Evaginatus: Management Using Novel Materials- A Case Report *Corresponding Author: Dr. Pradnya V. Bansode1 15 | Page was done by circumferential filling using hand K-files (Mani, Inc., Japan).During instrumentation, the canals were irrigated copiously with 2.5% sodium hypochlorite solution followed by final wash with normal saline to remove extruded hypochlorite through open apex if any. Drying of the canal was done with absorbent paper points (Dentsply, Maillefer). Triple antibiotic paste root canal dressing was performed and changed every 14 days followed by frequent root canal irrigation with hypochlorite and saline for the period of one month. The access cavity was temporized with the intermediate restorative material (IRM, Dentsply Caulk, Milford, USA). After one month patient was asymptomatic. Figure 1: Occlusal tubercle in mandibular pre molars.Figure 2: Diagnostic IOPAR, periapical radiolucency and open apex with respect to 35. The tooth was again isolated under rubber dam and the canals thoroughly irrigated with hypochlorite andsaline to wash out any remnants of triple antibiotic paste. DFDBA was mixed with normal saline to packable consistency. It was then carried to periapical area under radiographic guidance with the help of endodonticplugger having rubber stop at pre adjusted working length (fig 3). After having confirmed an adequate matrix mass of DFDBA, Biodentine was mixed and placed over DFDBA with the help of messing gun to getcompact plug of 3-4 mm (fig 4). Biodentine plug was allowed to set for 10-12 minutes after which reminder of canalwas obturated with guttapercha. Access opening was sealed with high copper silver amalgam.(fig 5).At 6 months follow up healing in periapical area can be appreciated (fig 6). Figure 3: DFDBA placement using pluggerFigure 4: Biodentine as an apical plug Figure 5: Post-operative radiographFigure 6: 6 months follow up
  • 3. Dense Evaginatus: Management Using Novel Materials- A Case Report *Corresponding Author: Dr. Pradnya V. Bansode1 16 | Page III. DISCUSSION Dens evaginatus is a tooth anomaly with considerable clinical significance causing occlusal interference.Maintaining cleanarea between the nodule and the tooth becomes difficult, and cariesis often found. There are high chances of pulp exposureduring early phases of root development, resulting in pulpnecrosis and incomplete root formation.The unusual tubercleor elevation on the tooth surface is the usual presentation butsometimes, due to fracture or attrition, no external evidenceof the malformation may be evident [7].Thus, early detection ofthese conditions is so important that preventivemanagementcan be started as early as possible. In the tooth/teeth with DEwith vital pulp, selective reduction of the opposing occludingteeth can be done or, in a situation where the tubercle hasfractured, it can be sealed with resin. In the case of DE withpulp exposure during the early phase of root development,pulpotomy is suggested. Ifthe pulp is necrotic, root end barrier in the case of theimmature apex and conventional root canal treatment shouldbe performed on the mature tooth [1]. Interappointment antimicrobialmedication can be advantageous to curtail bacterial regrowthand possibly even improve bacterial suppression [8]. Studiesreported the clinical effectiveness of triple antibiotic paste incases of apical periodontitis [9,10].The antibiotic combinationwas reported to bemore effective againstmixed bacterialflora as in infected root canal tested when compared withcalcium hydroxide, iodine potassium iodide, or iodoform[11]. Gomes-Filho et al. [12] evaluated the response andconcluded that triple antibiotic paste is a biocompatibleintracanal medicament. A study conducted byWindley et al.[9] observed that the use of triple antibiotic paste followingirrigation resulted in significant reduction in bacteria comparedto that of irrigation with sodium hypochlorite alone.Studies have demonstrated that predictable disinfection ofthe root canal systemcanbe achievedbyproper antimicrobialintracanal medication [13]. The major problem in cases of a wide open apex is the need to limit the material to the perforation, thus avoiding theextrusion of a large amount of material into the periodontal tissue [14,15]. A large volume of the extrudedmaterial may set before it disintegrates and get resorbed. This might result in the persistence of the inflammatory process, which may complicate or even prevent repair of the tissue [14,15] Using a matrix avoidsthe extrusion of the material into the periodontal tissues, reduces leakage in the sealing material and allowsfavorable response of the periodontal tissues.DFDBA is obtained from cortical bone of long bones due to its high content of bone inductive proteins and less antigenic activity than cancellous bone. Thecollagen matrix present in DFDBA acts as a scaffold that provides osteoconductive properties alone side theosteoinductive behavior [16]. BMPs are associated with the organic matrix of bone and embedded withinmineral content, so demineralised process in formation of DFDBA increases its bioavailability. BMPs attractmesenchymal stem cells and induce them to differentiate into chondrocytes leading into endochondral boneformation [17]. This property is helpful in healing of large periapical lesions. Taking into consideration all these advantages of DFDBA, we have used these materials as an apical barrier matrix over which Biodentine was placed proving success of this case. IV. CONCLUSION The communication of root canal to oral cavity due to the tubercle openingmay contributes to harboring virulent microorganismsthat succeeds in colonizing the canal and thus periapex. The clinician should be aware of such anomaliesand their consequences so that proper treatment modalitiescan be instituted. Early detection and careful treatment planning areneeded to prevent further complication of the condition. REFERENCES [1]. M. E. Levitan and V. T. Himel, “Dens evaginatus: literaturereview, pathophysiology, and comprehensive treatment regimen,”Journal of Endodontics, vol. 32, no. 1, pp. 1–9, 2006. [2]. S. Stecker and A. J. DiAngelis, “Dens evaginatus. A diagnosticand treatment challenge,”The Journal of the American DentalAssociation, vol. 133, no. 2, pp. 190–193, 2002. [3]. E. Dankner, D. Harari, and I. Rotstein, “Dens evaginatus ofanterior teeth. Literature review and radiographic survey of15,000 teeth,” Oral Surgery, Oral Medicine, Oral Pathology, OralRadiology, and Endodontics, vol. 81, no. 4, pp. 472–475, 1996. [4]. F. J.HillandW. J. Bellis, “Dens evaginatus and itsmanagement,”British Dental Journal, vol. 156, no. 11, pp. 400–402, 1984. [5]. C. E. Jerome and R. J. Hanlon Jr., “Dental anatomical anomaliesin Asians and Pacific Islanders.,” Journal of the California DentalAssociation, vol. 35, no. 9, pp. 631–636, 2007. [6]. G. S. Kocsis, A. Marcsik, E. L. K´okai, and K. S. Kocsis,“Supernumerary occlusal cusps on permanent human teeth,”ActaBiologicaSzegediensis, vol. 46, no. 1-2, pp. 71–82, 2002. [7]. A. Ayer, M. Vikram, and P. Suwal. “Dens Evaginatus: A Problem-Based ApproachCase Reports in Dentistry” Volume 2015, Article ID 393209 [8]. Z. Metzger, B. Basrani, and H. E. Goodis, “Instruments, materials,and devices,” in Cohen’s Pathways of the Pulp, K. M.Hargreaves and S. Cohen, Eds., pp. 223–282, Elsevier, Mosby,2011. [9]. [9] W. Windley III, F. Teixeira, L. Levin, A. Sigurdsson, and M.Trope, “Disinfection of immature teeth with a triple antibioticpaste,” Journal of Endodontics, vol. 31, no. 6, pp. 439–443, 2005. [10]. B. Thibodeau, F. Teixeira, M. Yamauchi, D. J. Caplan, and M.Trope, “Pulp revascularization of immature dog teeth withapical periodontitis,” Journal of Endodontics, vol. 33, no. 6, pp.680–689, 2007. [11]. R. C. Pallotta, M. S. Ribeiro, andM. E. D. L.Machado, “Determinationof the minimum inhibitory concentration of fourmedicaments used as intracanal medication,” Australian EndodonticJournal, vol. 33, no. 3, pp. 107–111, 2007.
  • 4. Dense Evaginatus: Management Using Novel Materials- A Case Report *Corresponding Author: Dr. Pradnya V. Bansode1 17 | Page [12]. J. E. Gomes-Filho,P.C.T.Duarte, C. B. deOliveira et al., “Tissuereaction to a triantibiotic paste used for endodontic tissue selfregenerationof nonvital immature permanent teeth,” Journal ofEndodontics, vol. 38, no. 1, pp. 91–94, 2012. [13]. C. J. Baumgartner, J. F. Siqueira, C. M. Sedgley, and A. Kishen,“Microbiology of endodontic disease,” in Ingle’s Endodontics 6, J.I. Ingle, L. K. Bakland, and J. C. Baumgartner, Eds., pp. 221–308,BC Decker Inc, Hamilton, Canada, 2008. [14]. Broon NJ, Bramante CM, Assis GF, Bortoluzzi EA, Bernardineli N, Moraes IG, et Healing of root perforations treated withmineral trioxide aggregate (MTA) and Portland cement. J Appl Oral Sci 2006;14:305-11. [15]. Holland R, Mazuqueli L, Souza V, Murata SS, Dezan E Jr. Influence of the type of vehicle and limit of obturation on apical andperiapical tissue response in dogs teeth after root canal filling with mineral trioxide aggregate. J Endod 2007;33:693-7. [16]. Drosos GI, Kazakos KI, Kouzoumpasis P &Verettas DA.(2007). Injury;38Suppl 4:S13-21. [17]. Esposito M, Piattelli M, Pistilli R, Pellegrino G. &Felice P. Eur J Oral Implantol 2010;3(4):297-305.