1. MOEY
National Forum on
Regulation, Use and Trade of
Biocontrol Agents (BCA) in Cambodian
Agriculture
July 28-29, 2015
Presented by: Mr. Sip Pagnasoley

2. Content
-UBB Information and Faculty of Agriculture
-Capacity Building
-Research activity
-Future

3. General Information of UBB
UBB is the public university in Cambodia, established in 2007 to provide
opportunities to students living in rural areas, especially in the north-western
part of Cambodia, to have access to higher education services in order to
develop their individual careers as well as to that of local communities while
also reducing knowledge gaps between the rural and urban.
UBB’s goal is to educate the leaders of tomorrow in a wide range of fields through
a balance of theory and practice.
It has five faculties and one institute include:
Faculty of Business Administration and Tourism,
Faculty of Agriculture and Food Processing,
Faculty of Sociology and Community Development,
Faculty of Arts, Humanities, and Education,
Faculty of Science and Technology and
Institute of Foreign Languages.

4. Faculty of Agriculture and Food Processing
Faculty of Agriculture and Food Processing is a big one of five faculties of
University of Battambang. We have 04 Departments:
-Department of Agricultural Sciences
-Department of Animal Science
-Department of Food Processing
-Department of Aquaculture.
Faculty, in this academic year, educates bachelor of 04 majors—Horticulture,
Animal Science(Livestock Production), Food Processing, Aquaculture.
Total number of staff: 26 staffs
The number of student involving, totally : 567 Students.

6. Organization Chart Dean
International Advisor
(KOICA, NRF, JICA, Fulbright ...ect)
Vice Dean
Department
Secretary
Research & Development, Division
Laboratory & Practical WorkFarm Research & Training Center
Department of Horticulture
Department of Animal Science
Department of Food Processing
Department of Aquaculture
Food Processing Laboratory
Animal Science Laboratory
Microbiology Laboratory
Grain Research &
Certification Center
Water Quality Laboratory
Soil Laboratory
Entomology Laboratory
Aquaculture Laboratory
Mushroom Laboratory
Practical Laboratory
Fiel Demonstration
General Basic Laboratory
Tissue Culture Laboratory
Live Stock
Aquaculture
Crops & Fruit Trees
Cassava Center
Pilot Plan
Rice Seed Production
Agricultural Machinery
Training Center

7. -Seed and Seedling born diseases of Vegetable crop.
-Production and use of Bio control agent Trichoderma sp.
-Production and Use of Bio Pesticides.
(In India and Nepal)
-Diseases Diagnosis Training(GDA)
-IPM Training 05 Junior staffs
-Bio-control and Production of Bio agent
-Biological Control on Crop Management
Capacity Building Supported by USAID

8.  Training: Ubon Ratchathani University
Isolate Trichoderma

9.  Training on Production of Bio-agents, Nepal

10.  Regional meeting, Philippine

11. Workshop at University of Battambang

12. Practical work in the lab(Training)

13.  Biological control Training

14.  Isolate bio agent from soil

15.  Practical by mixing Trichoderma spp with Compost

16.  Diluted Trichoderma spp

17.  Collect Soil sampling for isolate Bio-agent
Activity: Bio-agent research and testing at UBB

18.  Isolate Bio-agent from soil

19.  Testing using Bio-agent on Insect pest
Fungus: Metarhizium

20.  Vegetative multiplication Trichoderma

21. 05 species had been isolated
-Fungus
.Mycorrizhae
.Metarhizium anisoplias
.Aspergillus niger and
.Beauvaria bassiana
-Bacterial
.Steptomyces
.Pseudomonas
And We are producing Trichoderma harzianum

22. Effect of using Trichoderma h. on Celery Cabbage in
Sangker district, Tmorkol district and Battambang city,
Battambang province

23. Treatment(T) Description Using Of Trichoderma h.
1 T1 Battambang (UBB)
1kgTrichoderma spp.
Mixed Compost 100Kg
2 T2 Sangker
3 T3 Thmorkol
 Method of experiment
 The experiment was done at University of Battambang
 The experiment was conducted 3 treatments and 4 replication
 Complete Block Design (CBD)
 Treatments

24. 19.9
25.65
17.7
0
5
10
15
20
25
30
T1 T2 T3
(Tone)
Treatment
Celery Cabbage Yield (Chaysim)

25.  Data collection Trichoderma spp

26.  Method of experiment
 The experiment was done at University of Battambang
 The experiment was made 5 treatments and 4 replication
 Block design (RCBD)
 Treatments
បច្ច័យ(Tr) Description
1 T0 Compost 100 Kg
2 T1 Trichoderma h. 0.5 kg + compost 100 kg
3 T2 Trichoderma h. 1 kg + compost 100 kg
4 T3 Trichoderma h. 1.5 kg + compost 100 kg
5 T4 Trichoderma h. 2 kg+ compost 100 kg
6 T5 Trichoderma h. 2.5 kg+ compost 100 kg

27.  Cucumber yield
25
42.75 45.35 45
38.3
48.7
0
10
20
30
40
50
60
T0 T1 T2 T3 T4 T5
Tons
Treatment
Total yields/ha

30. Effective of Trichoderma h. to Decreasing
Chemical Fertilizer on Different Rice Varieties

31.  Methodology for experiment
 Location in Bakongre village, Prek Onlong commune, Ek phnom District.
 05 treatments and 04 Replications
 Block Design (RCBD)
 02 kg of Trichodema h mixing with compost 100 kg
DAP/ha+Trichoderma h.2 kg
Interaction
V1
Senkraw Ob
V2
Sen Pidor
V3
IR 66
V4
Chulsa
No DAP 100:50:00 (Recommedation)
No V1 No V2 No V3 No V4
N1 DAP75% of N០+trichoderma h.
N1 V1 N1 V2 N1 V3 N1 V4
N2 DAP50% of N០+trichoderma h.
N2 V1 N2 V2 N2 V3 N2 V4
N3 DAP25% of N០+trichoderma h.
N3 V1 N3 V2 N3 V3 N3 V4
N4 DAP 0%+Trichoderma h. N4 V1 N4 V2 N4 V3 N4 V4

32. បរិយាយ V1 V2 V3 V4 N0 N1 N2 N3 N4
High 86.8a 84.28b 67.64d 70.96c 79.51a 79.56a 77.78ab
76.08b
c
74.15c
Number 17.85b 16.73b 23.38a 22.34a 22.35a 21.44a 20ab
18.84b
c
17.75c
Flower 90.95a 90.4a 85.75c 86.6b 89ns 88.44ns 88.12ns
88.31n
s
88.25ns
Yield/ha 4.46 c 4.40 c 6.33a 5.14 b 5.71a 5.81a 5.06ab 4.57bc 4.26c

34.  Experiment on using different Substrate for Trichoderma spp
multiplication

35. Effective of Different Substrate for Trichoderma spp.
multiplication
T1: Broken rice
T2: Corn
T3: Rice
T4: Soybean
04 Replications

36. Effective of Different Substrate for Trichoderma spp.
Multiplication
Treatment Mycelium Day 1 Day 2 Day 3 Day 4 Day 5
T1
% 0 60 85 100 100
Color No White Light Dark Dark
T2
% 0 70 95 100 100
Color No White Light Dark Dark
T3
% 0 1 80 100 100
Color No White Light Dark Dark
T4
% 0 5 30 50 70
Color No White Light Dark Dark

37. COUNTING OF SPORE

39. 1.Trichoderma virid : 183 x106 spores/g
2.A : 100 x106 spores/g
3.B : 999 x106 spores/g
4.C : 368 x106 spores/g
5.D : 368 x106 spores/g
6.UBB : 340 x106 spores/g
7.E : 3 x108 spores/g
8.F : 12 x108 spores/g
 Results

41. Triangle
Ministry(MOEY, MAFF…ect) and Provincial: Strong
Support by providing budget, Land and
Coordination with Association, Company…ect.,

42. Future Need and Priority for AEC
-Strongly collaboration with partners as:
.Staff and student exchange program
.Sharing information and
.Joint research activity and research project.
-New Directorate General of Policy and Planning of MOEY

43. Thank You