1. Olga Golubkov
5282 Av. Connaught Montreal Quebec H4V 1X6
(438)321-2993) olga372003@yahoo.ca
OBJECTIVE Seeking a Technical Position in Biotech Industry or in Research
HIGHLIGHTS OF QUALIFICATIONS
o Over 17years of lab experience
o Extensive expertise in molecular biology and micro-biological methods
o Skilled in implementing science research and analysis
o Good communication and time-managing skills
o Fast learner, able to adapt quickly to a challenge and work hard under pressure to achieve set targets
o Team oriented with ability to work with individuals at all levels
o Confident, enthusiastic, articulate and responsible
COMPUTER SKILLS PowerPoint, Access, Adobe Photoshop,
Statistical Software
Main Gene identification programs, HIV drug resistance databases
LANGUAGES English, French, Hebrew, Russian (mother)
PROFESSIONAL EXPERIENCE
Methods of molecular biology
In Situ Hybridization Analysis (with radioactive and dig probes)
Synthesis of radiolabelled and dig- probe
PCR , real time PCR, nested PCR
Molecular cloning (plasmid DNA extraction and purification, ligation between foreign DNA and
plasmid vector, bacteria transformation)
RNA and DNA extraction and purification
Gel electrophoresis of DNA
DNA sequencing
Northern Blot Analysis
Isolation and maintenance of human mononuclear cells
Competent cell preparation
Blood’s plasma separation
Dark field microscopy
Sectioning of paraffin embedded objects on microtome
Microbiological methods
Isolation and maintenance of microbial cultures
Primary identification, maintenance of stock collections, pure culture techniques
Bacterial counting and dilution methods
Laboratory screening of microbial activity of different microorganisms’ groups
Screening of pesticides activity against pathogenic microorganisms of agricultural plants in laboratory and field
conditions
Botanic methods
Pollen grain extraction, coloring, growing under artificial condition, microscopic investigation
EMPLOYMENT HISTORY
Lady Davis Institute for Medical Research and McGill AIDS Centre Nov 2004 - July 2016
Jewish General Hospital, Montreal
Research Assistant
Detecting of HIV genomic mutations (HIV-1 drug resistance testing) using the TRUGENE HIV-1 genotyping
Kit, the OpenGene DNA Sequencing system and Virco ® TYPE HIV- genotype-analysis system
Isolation and maintenance of human mononuclear cells from peripheral blood
Laboratory documentation management
Final reports generating (Using Viroscore software)
Maintenance of the lab (registering and keeping the samples, ordering materials, stocking, maintaining a clean
work environment etc.)
2. Olga Golubkov
5282 Av. Connaught Montreal Quebec H4V 1X6
(438)321-2993) olga372003@yahoo.ca
Weizmann Institute of Science, Dept. of Molecular Genetics, Israel 2003 - 2004
Research Assistant
Analyses spatial and temporal patterns of expression of RUNX1&3 transcription factor in sectioned mouse
embryos using method of in situ RNA hybridization
Implemented PCR analysis of genome DNA
Managing routine operations in laboratory environment (ordered chemicals; prepared solutions, media and
buffers; maintained laboratory equipment)
Institute of Animal Science, Poultry Dept., Volcani Center, Israel 2002 - 2003
Research Associate
Studying molecular pathways of heat resistance in the chicken’s brain using method of in situ RNA
hybridization and Nothern analysis
Handling operations in laboratory environment (ordering chemicals; preparing solutions, media and buffers;
laboratory equipment maintaining)
Institute of Field and Garden Crops, Dept. of Vegetation, Volcani Center, Israel 2000 – 2001
Research Assistant
Studied physiological parameters of pollen on industrial and experimental varieties of vegetable crops in
laboratory and green house conditions
Implemented mathematical modeling of temperature conditions role in pollen development
Conducted microbiological analysis of different groups of microorganisms which inhabit agricultural plants in
greenhouse conditions and identified their relationships and interactions
Produced statistical analysis of obtained data
Institute of Hydraulic Engineering and Melioration, Kiev, Ukraine 1993 – 1999
Senior Technician
Studied relationships and interactions between microorganisms in various soil ecosystems under artificial
irrigation
Monitored artificial factors that affect the performance of microbial community in the field
Examined the influence of climatic conditions on productivity of agricultural plants
Initiated mathematical modeling of development of negative processes on soil under irrigation
Implemented development of computer database of different biotic soil parameters, prepared final reports
Institute of Plant Protection, Kiev, Ukraine 1987 – 1993
Technician
Studied relationships and interactions between different groups of microorganisms which inhabit agricultural
plants and examined their function in decreasing of damage caused by pathogenic fungi and bacteria
Researched pesticides role in agro-ecosystems and looked for ways of elimination of negative results of
pesticides use
Inspected microbial community with following antagonistic activity screening against main agricultural plants
pathogens that are being targeted for bio-pesticides development
Developed chemical and biological methods in plant protection
Developed mathematical models for forecasting of agricultural plants diseases
Created computer databases of different parameters of agricultural plants and their pests
EDUCATION
B.Sc. (Biology) 1982-1987
Ukrainian Agricultural Academy, Kiev, Ukraine, Dept. of Plant Protection
Courses in LDI
Workplace Hazardous Materials Information System (WHMIS) July , 2016
Biosafety and Safe Practices in Laboratories July 22, 2013
Workplace Hazardous Materials Information System (WHMIS) January 23, 2012
Courses in UQAM 2007 – 2008
Analytical chemistry
Methods of separations (chromatography)
Bioassays of contaminants
Spectroscopic analysis
References available upon request
3. Olga Golubkov
5282 Av. Connaught Montreal Quebec H4V 1X6
(438)321-2993) olga372003@yahoo.ca
