This presentation is a brief introduction to the histological techniques for Medical Laboratory (Biomedical Sciences) student!

1. Histological Techniques
Nuha Al Jabri
Oman College of Health Sciences

2. Objectives
What is Histology?
What are Histopathological Techniques?
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3. Histology
The microscopic study
of tissue of organs in
the body
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4. Bascially
When a doctor want to know what changes happens indise
his body, the doctor will ask for histological studies.

5. Histolopathological
techniques
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6. Biobsy
Operative procedure performed by the surgeon
or physician to obtain either a part (Incision
Biopsy) or the whole of tissue (Excision Biopsy).
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7. Initiation of Fixation
Process of preserving the tissue in “as life-like”
state as possible.
The most frequently used chemical is “Formalin”
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8. Registration of Specimens
An important clerical step of issuing “a unique”
number to the specimen.
This step prevent “mix-up” of specimens in later
stages8
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9. Grossing
Done by pathologist
Process of selecting the most likely are of
pathology for further processing and microscopic
studies.9
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10. Completion of Fixation (Re-Fixation)
To ensure that the tissue is properly and
completely fixed before further steps
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11. Processing
Dehydration
Removal of
water from
tissue.
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Clearing
Intermediate step.
Impregnation or
infiltration
Filling the tissue
with paraffin wax

12. Block making (Embedding)
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Process of surrounding the
impregnated tissue with paraffin wax

13. Section cutting (Microtomy)
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The steps used to slice the “processed
tissue” into thin slices.
The slices tissue called a section.

14. Staining and Mounting
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The process of allowing different colored
solutions (stains) to color different
components of the cell.

15. Reporting
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The pathologist studies the processed and
stained slide to identifies any abnormalities
that may be present in the tissue

16. THANKS!
Any questions?
All histological techniques will be
explained in depth through other
lectures.
Work Hard and enjoy the journey!
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