Experiment No.: 4 The Study of Effect of drugs on gastrointestinal motility. Pharmacology-3 (Practical) B.Pharm 3rd Year PCI Syllabus (New) Contents- •Aim •Reference •Requirements •Theory/Principle •Procedure •Observations •Result

1. The Study of Effect
of Drugs on
Gastrointestinal
Motility
BY
NAKUL DHORE
Pharmacology_III Practical

2. Experiment No.: 04
To study the effect of drugs on gastrointestinal motility.
Practical Book of Pharmacology_III by Dr. Rupesh
Gautam & Baljindar Singh NIRALI Publication [Page
No. 9-11]
Frog, physostigmine stock soln(1ug/ml), Atropine
stock soln(1ug/ml), Normal saline, frog board,
poppy seeds or tiny pieces of cork, stop watch,
surgical instruments
AIM_
REFERENCE
REQUIREMENTS

3. PRINCIPLE_ Intestinal motility is regulated by the enteric
nervous system of the gut & the activity of this
system can be modified by ANS. Hence effect of
sympathomimetic & parasymapthomimetic drugs on
intestinal motility can be studied by using isolated
pieces of intestine
Many Animal models can be employed to the
study. Guinea pig ileum is advantageous for the
purpose as it produces steady baseline for studying
effect of drugs. Rabbit intestine usually jejunum is
used for the effects of pendular movements.
In the present study Frog is used to achieve the
effect of drugs on gastric motility.

4. THEORY_ The movement of food particles in the oes
ophagus moves with the help of cilia. Likewise the
importance of mucociliary function has been
recognized in respiratory tract and of pulmonary
diseases such as asthma chronic bronchitis & in cystic
fibrosis. It has been verified that Acetylcholine
present in mucous membranes of trachea and Buccal
cavity aids in ciliary movements. Acetylcholine also
serves as local hormone with the help of choline
acetylase support the fact that Acetylcholine is in the
vicinity synthesized in the mucous membranes.

5. PROCEDURE_ • The frog is decapitated and pin to the frog board on
its back.
• The lower jaw is pinned upto the abdomen cutting
sufficiently the buccal cavity and exposing the
oesophagus. The buccal cavity is kept and the
opening of the oesophagus wet by irritating it with
normal saline.
• The distance travelled by the particle is assessed.
Two points are fixed that is one at start of the lower
jaw and other at the beginning of the oesophagus.
The distance is kept constant to measure the time
taken by the particle to move from a fixed point in
the lower jaw to the beginning of the oesophagus.

6. • A poppy seed (tiny pieces) of cork at the premarked spot in the
jaw. The stop watch is turned on and noted the time taken by the
object to reach the beginning of the oesophagus.This repeats
time to time for several minutes.
• Few drops of physiostigmine is put on the buccal cavity and after
10min. Step 4 is repeated and the time is noted down.
• The buccal cavity is washed with normal saline. A few drops of
atropine is put on the buccal cavity. After 10 min, step 4 is
repeated and time is noted down.
• The difference is found out in the time taken by the object to
move between the pre marked distance in the buccal cavity in the
presence saline physiostigmine and atropine.
CONTD...

7. CONTD…
POPPY SEEDS
DECAPITATED FROG
ANATOMY OF FROG

8. OESOPHAGUS OF FROG
SALINE SOLUTION PHYSOSTIGMINE ATROPINE
STOPWATCH

9. Sr.
No.
Treatment
Estimated
Distance(cm)
Time (sec)
R1 R2 R3
1. Saline 0.8 34 29 30
MEAN= 31
2. Physostigmine 0.8 14 14 14
MEAN= 14
3. Atropine 0.8 42 46 44
MEAN= 44
OBSERVATION_

10. Graphical Representation of the Effect of Drugs

11. INFERENCE_
Atropine increase the time taken by the
object to move from the previously marked point
in the lower jaw to reach the oesophagus and on
the other hand physiostigmine reduces the time
taken by the object to move from pre-marked
point in the lower jaw to reach the oseophagus.
SOURCES_ • www.labmonk.com
• Oxford University Press
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