1. Multicentre Sampling
Patient Data
•Clinical and demographic
•Collected using Case Report Forms (CRFs)
•Managed by Clinical Trials Research Unit
•Standardised across all centres
•Identical to a clinical trial
•Data:
•Quality Managed
•Transcribed into database
Prospective Biobanking Within a Biomarker Pipeline
-Part of an NIHR-funded Applied Research Programme
Michael Messenger1&2, Tobias Wind1&2, Douglas Thompson1&2, Catherine Sturgeon3, Marc Jones1, Andrew
Lewington1&2, Rosamonde Banks1 ,Peter Selby1&2, and collaborators
University of Leeds, UK1; Leeds Teaching Hospitals NHS Trust, UK2; Royal Infirmary of Edinburgh, UK3
Website: www.biomarkerpipeline.org
Introduction
Protein biomarkers quantifiable in human bodily fluids provide simple, accessible and cost-
effective tests for improved diagnosis, prognosis, treatment selection and disease monitoring.
However, the translation of biomarkers from basic research into the clinical laboratory is
inefficient and collaborative multidisciplinary approaches are required to expedite this process.
The National Institute for Health Research (NIHR) funded Biomarker Programme
(www.biomarkerpieline.org) assimilates health economists, statisticians, scientists and clinicians
into three interlinking workstreams addressing 1). methodology development, 2). sample banking
and intermediate marker assessment and 3). the ELUCIDATE biomarker randomised control
trial. Focussing on the second of these, the challenges and approaches involving multicentre
sample banking are highlighted here.
Programme BioBanking Summary
In total 4,680 patients, 12,340 samples and 304,400 aliquots
Programme Funded By:
Pre-Analytical Factors
Sample Collection & Processing
•Standard Operating Procedures (SOPs) used throughout
•Procedures developed in line with international standards and guidance (e.g. CLSI)
•Standardised sample collection packs used across all sites
•Samples labelled with unique double-decode identifiers, with 2D and 1D barcodes
Sample Storage & Cataloguing
Sample Pack Sample Processing Procedures
