1. Conjugation between an F+ cell and an F- cell
1
F plasmid integrates into
chromosome by recombination
2 Cells join via a conjugation pilus
3
Portion of F plasmid partially moves into
recipient cell trailing a strand of donor’s DNA
4
Conjugation ends with pieces of F plasmid and donor
DNA in recipient cell; cells synthesize
complementary DNA strands
5 Donor DNA and recipient DNA recombine making a
recombinant F- cell
Donor chromosome
Hfr cell
Pilus
Donor DNA
Part of F plasmid
F+ cell
F- recipientF+ cell (Hfr)F Plasmid
Incomplete F plasmid; cell remains F-
Recombinant cell (F-)

2. Conjugation between an F+ cell and an F- cell – With Labels Removed

3. Conjugation between an F+ cell and an F- cell – With Highlighted Part
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Donor Chromosomes

4. Conjugation between an F+ cell and an F- cell – With Highlighted Part
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Hfr Cell

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Recombinant Cell (F-)
Conjugation between an F+ cell and an F- cell – With Highlighted Part

6. " Add your Own Labels and Highlight Any section"
Donor chromosome

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