1. Curriculum Vitae (CV) of Dr. Md. Ashraful Haque
1. Name : MD. ASHRAFUL HAQUE
2. Name of father w ith address : Late Shaw kot Ali
Vill. Kanaidanga, PO. Chandrabash,
PS.Damurhuda, Dist. Chuadanga,
Bangladesh.
3. a) Date of birth
(according to Matric / SSC Certificate)
: 1st
November, 1973
b) Place of birth : Chuadanga, Bangladesh.
c) Nationality : Bangladeshi (by birth).
d) Permanent home address :Vill. Kanaidanga, PO. Chandrabash,
PS.Damurhuda, Dist. Chuadanga,
Bangladesh.
e) Present address : Professor, Department of Genetics and Plant Breeding,
Bangladesh Agricultural University, Mymensingh-
2202, Bangladesh.
f) Marital status : Married
4. Academic Career (fromMatriculation / SSC up to highest degree)
Name of
School / College /
University
Years attended Name of the Exams.
with subjects offered*
Division / Class &
Place obtained
Year of Obtaining
Certificate or DegreeFrom To
Karpashdanga High School,
Chuadanga.
1983 1988 SSC
(Science)
First Division 1988
Kushtia Government
College, Kushtia.
1988 1990 HSC
(Science)
First Division 1990
Bangladesh Agricultural
University, Mymensingh.
1991 1995 B.Sc.Ag (Hons) First Class & First
Position in order of
merit
1995
(Exam. held in 1999)
Bangladesh Agricultural
University, Mymensingh.
2000 2001 MS in Genetics and Plant
Breeding
First Class w ith
Distinction (Grade
A+
)
December, 2001
Tokyo University of
Agriculture and Technology,
Japan.
2006 2009 Doctor of Philosophy in
Biochemistry and
Biotechnology
Aw arded September , 2009
Ohio State University, USA. 2011 2011 Post doctoralresearch on
Molecular Genetics of
plant pathogens
Aw arded (Special
recognition aw ard by
the President of the
Ohio State University,
USA)
December, 2011
* Please see the Appendix-Ifor name of the examinations w ith subjects offered
5. Academic awards
* National Science and Technology aw ard offered by the Ministry of Science and Technology,
Bangladesh during 2013-2014
* Special recognition aw ard-2011 through post doctoralresearch by the Ohio State University, USA.
* Special recognition aw ard-2011 by IDB as an excellent researcher.
* IDB Merit Scholarship-2011 for a post doctoral researchin the Ohio State University, USA.
* Monbukagakushu Scholarship for Special3 years DoctoralProgram offered by the Ministry of
Education, Culture, Sports Science and Technology- Japan from October, 2006 to September, 2009.
* President Gold Medal aw ard offered by the Chancellor of Bangladesh AgriculturalUniversity during
February, 2003.
* Vice-Chancellor aw ard offered by the Vice-Chancellor of Bangladesh AgriculturalUniversity during
February, 2003.
* National Science and Technology aw ard offered by the Ministry of Science and Technology,
Bangladesh during 2000-2001.
* Shitabjan Memorial Trust aw ard offered by the Vice-Chancellor of Bangladesh Agricultural
University during 2000.
2. 6. Membership
* Life member of Plant Breeding and Genetics Society of Bangladesh.
* Life member of Bangladesh Association of Biotechnology.
* Life member of IDB Graduate Association in Bangladesh.
7. Training Program
* Successfully completed a course on General English from British Council,
Bangladesh.
*Successfully completed a Postgraduate Certificate course on Seed Technology.
*A training program offered by Graduate Training Institute on Scientific Report
Writing
* A training program offered by Graduate Training Institute on Teaching Methods
and Techniques
*A training program offered by Graduate Training Institute on Statistical Methods of
Analysis
* A training program offered by Graduate Training Institute on Project Cycle
Management
* A training program offered by Graduate Training Institute on Web page Design and
Hosting
* A training program offered by Graduate Training Institute on ISWS-510 Facilitating
Learning
* A field trip offered by Bangladesh AgriculturalUniversity on Agriculturalextension
w orkin field level
* A w orkshop on Women for Women offered by Bangladesh AgriculturalUniversity.
8. Research Interest
1. Functional analysis and characterization of plant genes related to defense and regulation mechanism against bi
otic and environmental stresses.
2. Expression pattern of biotic stress related transcription factors/genesand signaltransduction.
3. Functional and promoter activities of pathogenesis related genes.
4. In vitro study of some major crops for their screening based on yield contributing characters
5. Development and establishment of transgenic plants w ith suitable genes.
9. Expertise in Advanced Laboratory Techniques
Collection of data, statisticalanalysis of data, graphical representation, design of experiment, RNA, DNA and Protein
extraction & purification, gelelectrophoresis, Polymerase Chain Reaction (PCR), Reverse Transcriptase Polymerase
Chain Reaction (RT-PCR), Cloning, GFP and RFP fusion, Yeast one and tw o hybrid system, Sequencing and gene
silencing, Agrobacterium-mediated transformation in plants, Multiple sequence alignment by using BLAST , Designing
Primer and Vector, SDS-Page, Western blotting. Microscopy, CytologicalOperations, Embryo and anther culture of
plants and hybridization of crop plants etc.
10. Names and addresses of persons to whom a reference maybe made:
i) Lucy R. Stewart
Research Molecular Biologist USDA-ARS and Adjunct Assistant Professor
Departmet of Plant Pathology/OARDC,Selby Hall,Room No. 020,Wooster,Ohio-44691
The Ohio State University
USA
E-mail: lucy.stewart@ars.usda.gov
Phone:01-330-260-3834
Field of Specialization:Molecular Breeding and Genetics
ii) Hiroshi NYUNOYA
Professor of Gene Research Center
Tokyo University of Agriculture and Technology,Japan
E-mail: nuynoya@cc.tuat.ac.jp
Phone: 81-42-367-5563
Fax: 81-42-367-5840
3. 11. List of Publications of Dr. Md. Ashraful Haque
1. M. Chakraborty, T. Quddus, S. Rahman, M. A. K. Azad, M. R. Hossain, A. Hoque, M. K. Saha & M. A. Haque.
(2015). Molecular characterization of selected mutant lines of Onion (Allium cepa L.) against Purple Leaf Blotch
disease using SSR markers. American Jour. Expt. Agric. 8(4):261-267.
2. Sayed Raihanul Haider†
, Mohammad Rashed Hossain†
, Shanjida Rahman, Shirin Sultana, Tamanna Quddus,
Moutoshi Chakraborti, Aunamika Hoque, Md Hasan Shahriar and Md AshrafulHaque*. (2015). In Vitro plantlet
regeneration of four localgarlic (Allium sativum) accessions of Bangladesh. British Biotech. Jour. 8(3): 1-12).
3. Shanjida Rahman, Md. Mukul Mia, Tamanna Quddus, LutfulHassan and Md. Ashraful Haque. (2015) Assessing
Genetic diversity of Maize (Zea mays L.) genotypes for agronomic traits. Res. Agric. Livest. Fish. 2(1): 1-8.
4. S. Rahman, U. Mondal, M. M. Mia, L. Hassan and M.A. Haque (2014). Wsm gene mediated resistance regulation
in corn (Zea mays L.) viruses. 9th
Bienn. Con. PBGSB, Dhaka. P. 75-76.
5. Md. AshrafulHaque and Mou, D. R. (2015). Screening of Tomato genotypes against Tm 1 gene for Tomato
mosaic virus resistance. 2nd
ICBHA-GNOBB, Univ. Dhaka. P.108-110.
6. S. Rahman, U. Mondal, M. M. Mia, L. Hassan and M.A. Haque (2014). Wsm gene mediated resistance regulation
in corn (Zea mays L.) viruses. 9th
Bienn. Con. PBGSB, Dhaka. P. 75-76.
7. M. Chakraborty, M. A. K. Azad, S. N. Begum, M. M. Islam, A. Hoque, M. K. Saha and M. A. Haque . (2014).
Molecular characterization of selected mutant lines of Onion against Purple Blotch using SSR markers. 9th
Bienn.
Con. PBGSB, Dhaka. P. 78.
8. M. A. Haque, Haider, S. R., A. Hoque & Chakraborty, M. (2013). Screening of Local Garlic Strains and production
of Chromosomal Variants under in vitro condition. BAU. Res. Prog. (2013) p.32.
9. Lucy R. Stew art • Md. AshrafulHaque , Mark W. Jones • Margaret G. Redinbaugh. (2013). Response of maize
(Zea mays L.) lines carrying Wsm1, Wsm2 and Wsm3 to the potyviruses Johnsongrassmosaic virus and Sorghum
mosaic virus. Mol. Breed. 31:289–297 DOI 10.1007/s11032-012-9789-5.
10. Haque, M. A., Shanjida, R., Mou, D. R., Moutoshi, C. & Aunamika, H. (2013). Low- cost Eco-friendly Virus free
Maize cultivation for poverty alleviation and food security in Bangladesh through Molecular marker aided Breeding
.BAU Res. Prog. (2013) p.39.(A very preliminary report in Bangladesh).
11. Lucy R. Stewart • Md. Ashraful Haque, Mark W. Jones • Margaret G. Redinbaugh. (2012). Response of maize
(Zea mays L.) lines carrying Wsm1, Wsm2 and Wsm3 to the potyviruses Johnsongrass mosaic virus and Sorghum
mosaic virus. American Phytopath. Soc. (2012).
12. Md. Ashraful Haque, Mark W. Jones, M. G., Redinbaugh and Stew art L.R. (2011). Screening of corn (Zea mays
L.) genotypes w ith introgressed Wsm loci for resistance to the potyviruses Johnsongrass mosaic virus (JGMV) and
Sorghum mosaic virus (SrMV). Sixth Annu. Res. Exp.-2011 by the Ohio State University, USA.
13. Mayumi Takano, Md. Ashraful Haque, Shota Odaira1, Keiko Nakata, Nobumitsu Sasaki and Hiroshi Nyunoya.
(2013). Overexpression of a tobacco Dof transcription factorBBF1 stimulates the transcription of the tobacco mosaic
virus resistance gene
N and defense-related responses including ROS production.. Plant. Biotech. 30, 37-46 DOI:
10.5511/plantbiotechnology.12.1207a
14. M. A. Haque, M. A. Mahmud, M. M. Islam and S. N. Begum. (2012). Identification of introgressed rice lines of
Binadhan-7×FL-378 under salt stress through SSR markers. J. Bangladesh Agril. Univ. 10(1): 49–54.
15.Mayumi, T., Odaira, S., Md. A. Haque, Sasaki, N. and Nyunoya, H. (2011). Overexpression of Dof transcription
factor accelerates hypersensitive response to the elicitor of Tobacco Mosaic Virus through the upregulation of the
tobacco resistance gene N. 8th
Solanaceae and 2nd
Cucurbitaceae joint conference, Kobe,Japan.
16. Sasaki, N., Haque, M. A., Mayumi, T., Odaira, S. and Nyunoya, H. (2011).Transcriptional transactivation of the
tobacco resistance gene N for Tobacco Mosaic Virus through its own product N protein. World Congress of Microbes-
2011, Beijing, China.
17. Md. Ashraful Haque, Nobumitsu Sasaki, Hiromi Kanegae, Seisuuke Mimori, Jun-Shan Gao and Hiroshi
Nyunoya. (2009). Identification of a Tobacco mosaic virus elicitor-responsive sequence in the resistance gene N.
Physiol Mol Plant Pathol 73, 101-108.
18. Haque, M. A.,Yasmin M. R., Hossain, M. S. Mia, M. S. and M. S. Kabir. (2010). Effect of growth hormones on
micropropagation of Garlic. Bangladesh J. crop. Sci. 21 (1): 19-23.
4. 19. Sasaki, N., Haque, M. A., Matsumaru, M., Odara, S., Nyunoya, H. (2010).Involvement of tobacco transcription
factor Dof proteins in the promoter activation of the resistance gene N against Tobacco mosaic virus. 21st
International Conference on Arabidopsis research held in Yokohama, Japan,p-65.
20. Haque, M. A., Yasmin, M. R., Kabir, A. T. M. A. , Firoz, S. M. S.and Jamal, M.R. (2010). In vitro regeneration and
transformation of Chickpea (Cicer arietinum L.) via grafting. Bangladesh J. crop. Sci. 21 (1&2):151-158.
21. M. M. Khatun, Hossain, M. S., Haque, M. A. and M. Khalekuzzaman. (2010). In Vitro propagation of Citrullus
Lanatus Thumb. From nodal explants culture. J. Bangladesh Agril. Univ. 8(2):203-206.
22. Md. Ashraful Haque, Nobumitsu Sasaki, Hiromi Kanegae, Jun-Shan Gao and Hiroshi Nyunoya. (2009). Elicitor
responsive 20-bp element of the Tobacco mosaic virus resistance gene N. XIV international congress on Mol. Plant
Microbe Inter. (MPMI), p- 72.
23. Md. Ashraful Haque, Nobumitsu Sasaki, Hiromi Kanegae, Jun-Shan Gao and Hiroshi Nyunoya. (2009). Study
on the regulation of the elicitor-responsive expression of Tobacco mosaic virus resistance. The international
congress on Molecular Biology held in Meiji, Japan.
24. Md. Ashraful Haque, Nobumitsu Sasaki, Matsumaru Masamichi and Hiroshi Nyunoya. (2009). Functional
involvement of tobacco Dof protein in the resistance to Tobacco mosaic virus.The international congress on
Biochemistry and Molecular Biology held in Yokohama, Japan.
25. Haque, M. A., Yasmin M. R., Rajesh, S and Hassan, L. (2009). In vitro screeing of rapeseed and mustard
genotype for salt tolerance. Bangladesh J. Enviorn. Sci. 17:74-79.
26. Haque, M. A., Yasmin M. R., Dey, R and Hassan, L. (2009). In vitro regeneration of rice. Bangladesh J. Enviorn.
Sci. 17: 92-97.
27. Yasmin M. R. and Haque, M. A. (2009). Micropropagation through stem disc culture and its growth parameters
affecting bulblet formation of garlic. Bangladesh J. Enviorn. Sci. 16: 243-248.
28. Md. Ashraful Haque, Nobumitsu Sasaki and Hiroshi Nyunoya. (2008). Identification of a cis regulatory element
of the Tobacco mosaic virus resistance gene N. The international congress on Biochemistry and Molecular Biology
held in Kobe, Japan, p- 501.
29. Md. Ashraful Haque. (2007). Analyses of virus resistance N gene promoter of Nicotiana tabacum. A congress
arranged by the United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology held
in Chiba, Japan.
30. Haque, M. A. and M. R. Yasmin. (2006). Determination of total Sulphur in local cultivars of Garlic. Bangladesh J.
Crop. Sci.17 (1) 251-255.
31. Haque, M. A. and M. R. Yasmin. (2006).Sulfur utilization and Genotype- Environment interaction in Garlic.
Bangladesh J. Enviorn. Sci. 12 (1): 150-154.
32. Hossain, M. S., M. Khalekuzzaman, O. I. Joarder, Haque, M.A. and M. M. Rahman. (2006). Multivariate Analysis
in Rice Genotypes. Bangladesh J. Crop. Sci.17 (1) 7-12.
33. Yasmin, R., Haque, M. A. and Hassan, L. (2005).Comprehensive in vitro regeneration and screening of virus free
Garlic cultivars. J. Bangladesh. Soc. Agril. Sci.Technol. 2 (3 and 4):125-128.
34. Haque, M.A. and M.S. Alam. (2004). In vitro shoot regeneration and Plantlet formation in Garlic. J. Bangladesh.
Soc. Agril. Sci. Technol. 1 and 2(1): 54-60.
35. Haque, M.A. (2004). Chromosome Identification in Papaya (Carica papaya). J. Bangladesh Agril. Univ. 2(1):15-
20.
36. Haque, M.A. (2003). Production of chromosomal variant in Garlic using colchicine under in vitro condition.
Progres. Agric. 14(1 and 2): 57-60.
37. Haque, M. A. (2003). Efficacy of sucrose on direct regeneration and rapid bulblet formation in Garlic (Allium
sativum). Bangladesh J. Enviorn. Sci. 9(2): 205-211.
38. Haque, M. A., Ahmad, Q.N. and Alam, M. S. (2002). Somatic embryogenesis and formation of microbulbil under
in vitro regeneration of Garlic (Allium sativum). Bangladesh J. Enviorn. Sci. 8: 87-90.
39. Haque, M. A., Nath, U.K, Ahmad, Q.N and Alam, M.S. (2003). Effect of 2, 4-D and BAP on in vitro regeneration
of Garlic (Allium sativum). Online J. Biol. Sci. 2(12): 771-774.
5. 40. Hossain, M.A., Karim, M.R., Begum, S., Hossain, M.A and Haque, M. A. (2002). Effect of Cephalexin on sex
expression, fruit development and yield of Cucumber (Cucumis sativusL.). Online J. Biol. Sci. 2 (10): 656-658.
41. Datta, S., Rahman. M.M., Haque, M. A., Islam, M.M. and Mollah, M.F.A.(2002). Chromosomal studies on Ompok
pabda (Hamilton). Bangladesh J. Enviorn. Sci. 8:179-182.
42. Takano,M., Odaira,S., Haque, M. A., Nakata,K., Sasaki, N., and Nyunoya, H. (2013). Overexpression of the Dof
protein in tobacco leads to transcriptional activation of the resistance gene N, suggesting potential roles in the
regulation of hypersensitive response to TMV. ICAR-2013, Univ. West. Aus. held in 24-28 June, 2013.
Appendix-I:Name of the Examinations withSubjects Offered
Name of the Examinations Subjects offered
Secondary School Certificate
(SSC)
Bengali, English, General Mathematics, Geography, General Science (Ist & 2nd paper),
Elective Mathematics and Religious Education.
Higher Secondary Certificate
(HSC)
Bengali, English, Physics, Chemistry, Mathematics and Biology.
Bachelor of Science in
Agriculture (BSc Ag)
Agronomy, Soil Science, Horticulture, Crop Botany, Entomology, Plant Pathology,
Genetics & Plant Breeding, Agricultural Extension Education, Agricultural Chemistry,
Biochemistry, Chemistry, Agricultural Economics, Animal Husbandry, Farm Mechanics,
Agricultural Statistics, and Rural Sociology.
Master of Science (MS) in
Genetics and Plant Breeding
Cytology and Embryology, Biometrical and Population Genetics, Crop Evolution and
Plant Genetic Resources, Cytogenetics, Distant Hybridization and Chromosome
Manipulation, Biotechnology and Genetic Engineering, Principles of Plant Breeding,
Molecular and Developmental Genetics, Molecular Breeding, Molecular Genetics,
Experimentation and Data Analysis.
Thesis Title: Individual chromosome identification through karyotype analysis and
production of tissue culture regenerants in garlic.
Doctor of Philosophy in the
division of Applied Biological
Science.
Post doctoral research on
Molecular Genetics of plant virus
resistance in Maize
Chromosomal evolution and gene expression in higher plant, Molecular aspects in plant-
pathogen interaction, Structure and function of extracellular matrix, Bioactive material
chemistry, Intermolecular interaction and super molecular chemistry, Genome mapping
and breeding in crop plants, Versatile function of RNA, Carbon reduction w ith forest
resources and other courses offered by the United Graduate School of Agricultural
Science at Tokyo University of Agriculture and Technology in collaboration with other
member Universities.
Thesis Title: Study on the regulation of the elicitor-responsive expression of Tobacco
mosaic virus resistance gene N
Post doctoral research mainlyfocused on Corn virus resistance mechanism