If the binding activity was purified- suggest two experimental approac.docx
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If the binding activity was purified, suggest two experimental approaches that could be taken to verify that this factor is in fact a transcription factor? Explain. (4 marks) An electrophoretic mobility shift assay (EMSA) was performed using a radiolabeled DNA fragment from the sequence upstream of gene X . This DNA probe was incubated with (+) or without (-) nuclear extract isolated from tissues A (bone); B (lung); C (brain); and D (skin). The DNA-protein complexes were then fractionated on nondenaturing polyacrylamide gels. The gels were exposed to autoradiographic film; the results are presented in the figure. .
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ICD-10-CM/PCS coders
Nurses
Physicians Painted Valley, USA
Montero, Elena C Dr. Amber Shephard 126 #706203
PREPARTUM STANDING ORDERS
1.
2.
3.
Check fetal heart tones and vital signs.
Urinalysis
CBC
4
Call physician.
Perineal prep Fleets or soft suds enema as ordered. h.
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7.
NPO except for ice chips.
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bleeding, or fetal distress.
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Amber Shephard
Delivery Room
11/14/xx
I 2 Pitucin U10 in IV after delivery of placenta. If no TV, Pitocin U10 IM.
Amber Shephard
11/14/xx S:31pm Adruit and follow routine
orders. Breast feeding Amber Shephard
11/14/xx 11:30pm Delivered 23 year old
99. No lacerations.Normal vaginal delivery.
G2P1 NSVD 7 lb. 140z, female Apgars Female infant with Apgars of 9 and 9 in LOA.Estimate blood Inss: 300 ml. Afchrile Placenta delivered spontaneously.
Midline episiotomy with repaired with Ethilon.
Amber ShephardPainted Valley Antepartum testing
Sonography
Amniocentesis
Age under 16 or over 40 Gravida 1 with age 35-
Rh negative
Rubella exposure
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History of High Risk Complications
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Cesarean section
Stillborn or 3 or more abortions
Neonatal death
Live births under 5 pounds Toxemia or hypertensive disease
Immediate infant distress injury None
Diabetes mellitus
Genital infection
Urinary tract infection
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Over 2 weeks past FDC
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Date
11/14/xx 125/60
Time
BP
Temp
98.8
20
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Hour
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11/14
Date
Membranes
intact
ruptured 165
Height
Weight
Other
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consents
Signed
Condition:
Good
Fair
Critical
Gr
Para
L Ab 0 Stillborn
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LDC neg
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Current Medications None
56"
None
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OK
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ICD-10-CM/PCS coders
Nurses
Physicians Painted Valley, USA
Montero, Elena C Dr. Amber Shephard 126 #706203
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1.
2.
3.
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Urinalysis
CBC
4
Call physician.
Perineal prep Fleets or soft suds enema as ordered. h.
5.
7.
NPO except for ice chips.
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bleeding, or fetal distress.
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Delivery Room
11/14/xx
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11/14/xx S:31pm Adruit and follow routine
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99. No lacerations.Normal vaginal delivery.
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Amber ShephardPainted Valley Antepartum testing
Sonography
Amniocentesis
Age under 16 or over 40 Gravida 1 with age 35-
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Cesarean section
Stillborn or 3 or more abortions
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Obstetric Admission
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11/14/xx 125/60
Time
BP
Temp
98.8
20
Resp Induction
140)
yes yes
Lahor
No
Hour
Onsel 11/14 Date 0835 time 0800 time
Tast meal
11/14
Date
Membranes
intact
ruptured 165
Height
Weight
Other
Yes
consents
Signed
Condition:
Good
Fair
Critical
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Para
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LNMP
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LDC neg
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No
Current Medications None
56"
None
Allergies
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OK
Comments:
OK
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Prep
Enemar
5:15
LAB:
5:15
IV:
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time
FT.
Antepartum testing
Sonography
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Rubella exposure
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Cesarean section
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11/14/xx 125/60
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BP
Temp
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20
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