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Author information
MARY A. MULLEN,1HONGBING WANG,2KENT WILCOX,1TIM HERMAN2
ABSTRACT
The structure of the Max:DNA complex was investigated by cross-linking Max to a series of photoactive oligonucleotides. A single photoactive aryl azide was
introduced into oligonucleotides at defined positions downstream from the specific CACGTG binding site. Purified Max homodimers bound to and were cross-
linked to oligonucleotides containing a photoactive group either 2, 5, 8, or 11 bp downstream from the binding site. Further analysis revealed that an
amino-terminal chymotryptic peptide of Max was cross-linked to the oligonucleotide containing a photoactive probe 11 bp downstream from the specific
binding site. This result is consistent with the recent crystal structure of the Max:DNA complex (Ferré-D'Amaré et al., 1993) and further suggests that
amino acid residues near the amino-terminus of Max are in close proximity to a region of DNA that is separated from the core binding site by one turn of
the double helix.
The grapevine basic helix-loop-helix (bHLH) transcription factor positively modulates CBF-pathway and confers tolerance to cold-stress in
Arabidopsis
Weirong Xu, Ningbo Zhang, Yuntong Jiao, Ruimin Li, Dongming Xiao, Zhenping Wang
Molecular Biology Reports. Aug 2014, Vol. 41, No. 8: 5329-5342
CrossRef
Modification of nucleic acids using [3 + 2]-dipolar cycloaddition of azides and alkynes
A. V. Ustinov, I. A. Stepanova, V. V. Dubnyakova, T. S. Zatsepin, E. V. Nozhevnikova, V. A. Korshun
Russian Journal of Bioorganic Chemistry. Jul 2010, Vol. 36, No. 4: 401-445
CrossRef
Synthesis and evaluation of oligodeoxyribonucleotides containing an aryl(trifluoromethyl)diazirine moiety as the cross-linking probe:
photoaffinity labeling of mammalian DNA polymerase
T. Yamaguchi, K. Suyama, K. Narita, S. Kohgo, A. Tomikawa, M. Saneyoshi
Nucleic Acids Research. Jun 1997, Vol. 25, No. 12: 2352-2358
CrossRef
[37] Photochemical cross-linking assay for DNA tracking by replication proteins
Blaine Bartholomew, Rachel L. Tinker, George A. Kassavetis, E. Peter Geiduschek
. Jan 1995: 476-494
CrossRef
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Characterization of a Max:DNA
Complex by Cross-Linking to
Photoactive Oligonucleotides
MARY A. MULLEN, HONGBING WANG, KENT
WILCOX, and TIM HERMAN
DNA and Cell Biology. May 1994, Vol. 13,
No. 5: 521-530
PAY PER VIEW DNA and Cell Biology -
13(5):521-530; Characterization of a Max:DNA
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