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Martina Zivcakova - Opticke vlastnosti vcelyho pylu

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Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Optick´e vlastnosti vˇcel´ıho pylu Autor: Martina ˇZivˇc´akov´a ˇSkolitel’ka: Ing. Anna Korb´aˇrov´a, Ph.D. ´Ustav fyziky a meˇric´ı techniky Vysok´a ˇskola chemicko–technologick´a v Praze 26.6.2014 Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 1 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Osnova Obnˆoˇzkov´y pel’ Ciel’ pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 2 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Obnˆoˇzkov´y pel’ Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 3 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 4 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Ciel’ pr´ace Meranie farebn´eho rozptylu a homogenity/heterogenity jednotliv´ych obnˆoˇzok z dan´eho botanick´eho druhu • inform´acia o farebnom rozptyle jednotliv´ych botanick´ych druhov • homogenita/heterogenita povrchu, stredu a obnˆoˇzky rozpustenej v MeOH Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 5 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Ciel’ pr´ace Meranie farebn´eho rozptylu a homogenity/heterogenity jednotliv´ych obnˆoˇzok z dan´eho botanick´eho druhu • inform´acia o farebnom rozptyle jednotliv´ych botanick´ych druhov • homogenita/heterogenita povrchu, stredu a obnˆoˇzky rozpustenej v MeOH Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 5 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Ciel’ pr´ace Meranie farebn´eho rozptylu a homogenity/heterogenity jednotliv´ych obnˆoˇzok z dan´eho botanick´eho druhu • inform´acia o farebnom rozptyle jednotliv´ych botanick´ych druhov • homogenita/heterogenita povrchu, stredu a obnˆoˇzky rozpustenej v MeOH Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 5 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Ciel’ pr´ace Meranie farebn´eho rozptylu a homogenity/heterogenity jednotliv´ych obnˆoˇzok z dan´eho botanick´eho druhu • inform´acia o farebnom rozptyle jednotliv´ych botanick´ych druhov • homogenita/heterogenita povrchu, stredu a obnˆoˇzky rozpustenej v MeOH Meranie farebn´ych parametrov pomocou UV-VIS spektrometrie • porovnanie farebn´ych parametrov z´ıskan´ych UV-VIS spektrometriou a obrazovou anal´yzou Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 5 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Ciel’ pr´ace Meranie farebn´eho rozptylu a homogenity/heterogenity jednotliv´ych obnˆoˇzok z dan´eho botanick´eho druhu • inform´acia o farebnom rozptyle jednotliv´ych botanick´ych druhov • homogenita/heterogenita povrchu, stredu a obnˆoˇzky rozpustenej v MeOH Meranie farebn´ych parametrov pomocou UV-VIS spektrometrie • porovnanie farebn´ych parametrov z´ıskan´ych UV-VIS spektrometriou a obrazovou anal´yzou Meranie antioxidaˇcnej kapacity • korel´acia farebn´ych parametrov s v´ysledkami merania antioxidaˇcnej kapacity Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 5 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT CIE L∗ a∗ b∗ Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 6 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Meranie farebn´eho rozptylu Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 7 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Meranie farebn´eho rozptylu Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 8 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Meranie farebn´eho rozptylu Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 9 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Homogenita verzus heterogenita - Lipa (A) - obnˆoˇzka, (B) - povrch obnˆoˇzky, (C) - MeOH extrakt, (D) - stred obnˆoˇzky Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 10 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Homogenita verzus heterogenita - Poh´anka 1 (A) - obnˆoˇzka, (B) - povrch obnˆoˇzky, (C) - MeOH extrakt, (D) - stred obnˆoˇzky Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 11 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT UV-VIS spektrometria a obrazov´a anal´yza Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 12 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT UV-VIS spektrometria a obrazov´a anal´yza Porovnanie parametru L∗ s OA a UV-VIS Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 13 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT UV-VIS spektrometria a obrazov´a anal´yza Porovnanie parametru a∗ s OA a UV-VIS Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 14 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT UV-VIS spektrometria a obrazov´a anal´yza Porovnanie parametru b∗ s OA a UV-VIS Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 15 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Met´oda ABTS Princ´ıp met´ody ABTS HX−Fe3+ + H2O2 → X−[Fe4+ = O] + H2O ABTS R + X−[Fe4+ = O] → ABTS R ∗+ + HX−Fe3+ kde HX−Fe3+ je metmyoglob´ın, X−[Fe4+ = O] je ferrylmyoglob´ın, ABTS R je 2,2’-azinobis(3-ethyl-2,3-dihydrobenzothiazol-6-sulfon´at). Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 16 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Met´oda ABTS Princ´ıp met´ody ABTS HX−Fe3+ + H2O2 → X−[Fe4+ = O] + H2O ABTS R + X−[Fe4+ = O] → ABTS R ∗+ + HX−Fe3+ kde HX−Fe3+ je metmyoglob´ın, X−[Fe4+ = O] je ferrylmyoglob´ın, ABTS R je 2,2’-azinobis(3-ethyl-2,3-dihydrobenzothiazol-6-sulfon´at). Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 16 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Met´oda ABTS Princ´ıp met´ody ABTS HX−Fe3+ + H2O2 → X−[Fe4+ = O] + H2O ABTS R + X−[Fe4+ = O] → ABTS R ∗+ + HX−Fe3+ kde HX−Fe3+ je metmyoglob´ın, X−[Fe4+ = O] je ferrylmyoglob´ın, ABTS R je 2,2’-azinobis(3-ethyl-2,3-dihydrobenzothiazol-6-sulfon´at). Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 16 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Met´oda ABTS Princ´ıp met´ody ABTS HX−Fe3+ + H2O2 → X−[Fe4+ = O] + H2O ABTS R + X−[Fe4+ = O] → ABTS R ∗+ + HX−Fe3+ kde HX−Fe3+ je metmyoglob´ın, X−[Fe4+ = O] je ferrylmyoglob´ın, ABTS R je 2,2’-azinobis(3-ethyl-2,3-dihydrobenzothiazol-6-sulfon´at). Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 16 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Met´oda ABTS Princ´ıp met´ody ABTS HX−Fe3+ + H2O2 → X−[Fe4+ = O] + H2O ABTS R + X−[Fe4+ = O] → ABTS R ∗+ + HX−Fe3+ kde HX−Fe3+ je metmyoglob´ın, X−[Fe4+ = O] je ferrylmyoglob´ın, ABTS R je 2,2’-azinobis(3-ethyl-2,3-dihydrobenzothiazol-6-sulfon´at). Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 16 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Met´oda ABTS Princ´ıp met´ody ABTS HX−Fe3+ + H2O2 → X−[Fe4+ = O] + H2O ABTS R + X−[Fe4+ = O] → ABTS R ∗+ + HX−Fe3+ kde HX−Fe3+ je metmyoglob´ın, X−[Fe4+ = O] je ferrylmyoglob´ın, ABTS R je 2,2’-azinobis(3-ethyl-2,3-dihydrobenzothiazol-6-sulfon´at). Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 16 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Met´oda ABTS Princ´ıp met´ody ABTS HX−Fe3+ + H2O2 → X−[Fe4+ = O] + H2O ABTS R + X−[Fe4+ = O] → ABTS R ∗+ + HX−Fe3+ kde HX−Fe3+ je metmyoglob´ın, X−[Fe4+ = O] je ferrylmyoglob´ın, ABTS R je 2,2’-azinobis(3-ethyl-2,3-dihydrobenzothiazol-6-sulfon´at). Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 16 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Met´oda ABTS Princ´ıp met´ody ABTS HX−Fe3+ + H2O2 → X−[Fe4+ = O] + H2O ABTS R + X−[Fe4+ = O] → ABTS R ∗+ + HX−Fe3+ kde HX−Fe3+ je metmyoglob´ın, X−[Fe4+ = O] je ferrylmyoglob´ın, ABTS R je 2,2’-azinobis(3-ethyl-2,3-dihydrobenzothiazol-6-sulfon´at). Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 16 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Met´oda ABTS Princ´ıp met´ody ABTS HX−Fe3+ + H2O2 → X−[Fe4+ = O] + H2O ABTS R + X−[Fe4+ = O] → ABTS R ∗+ + HX−Fe3+ kde HX−Fe3+ je metmyoglob´ın, X−[Fe4+ = O] je ferrylmyoglob´ın, ABTS R je 2,2’-azinobis(3-ethyl-2,3-dihydrobenzothiazol-6-sulfon´at). Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 16 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Meranie antioxidaˇcnej kapacity Pr´ıprava materi´alu • uk´aˇzka roztriedenia podl’a farebnosti pre mak, slneˇcnicu a v´rbu Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 17 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Meranie antioxidaˇcnej kapacity Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 18 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT V´ysledky Meranie farebn´eho rozptylu 1 Z´ıskali sme predstavu o farebnej jednote: • jednotn´a - facelia, • jednotn´e s odch´ylen´ymi bodmi - lipa, ag´at, poh´anka 1,2 a mak, • v¨aˇcˇs´ı rozptyl - d’atelina plaziv´a, poh´anka 3, • v¨aˇcˇs´ı poˇcet farebn´ych skup´ın - v´rba, repka, slneˇcnica. Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 19 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT V´ysledky Meranie farebn´eho rozptylu 1 Z´ıskali sme predstavu o farebnej jednote: • jednotn´a - facelia, • jednotn´e s odch´ylen´ymi bodmi - lipa, ag´at, poh´anka 1,2 a mak, • v¨aˇcˇs´ı rozptyl - d’atelina plaziv´a, poh´anka 3, • v¨aˇcˇs´ı poˇcet farebn´ych skup´ın - v´rba, repka, slneˇcnica. 2 Na z´aklade farby sa n´am oddelili obnˆoˇzky do nasleduj´ucich farebn´ych skup´ın: • ˇzlt´a: lipa, mak, repka, ˇcast’ slneˇcnice a poh´anka 1, 2, • zelen´a: repka, ag´at, v´rba, ˇcast’ slneˇcnice a poh´anka 1, 2, • ˇcerven´a: poh´anka 3, ˇcast’ slneˇcnice a maku, • modrofialov´a: facelia. Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 19 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT V´ysledky Porovnanie v´ysledkov z obrazovej anal´yzy a UV-VIS pre parametere L∗ , a∗ a b∗ vych´adzaj´uce z modelu CIE L∗ a∗ b∗ Najlepˇsia zhoda pre parametre: • L∗ - lipa, poh´anka 1, 2 a d’atelina plaziv´a, • a∗ - poh´anka 3, • b∗ - lipa, d’atelina plaziv´a a ag´at, • korelaˇcn´y koeficient pre parameter L* = 0,38, a* = 0,81 a b* = 0,68. Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 20 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT V´ysledky V´ysledky porovnania parametru L∗ z modelu CIE L∗ a∗ b∗ z obrazovej anal´yzy z merania antioxidaˇcnej kapacity Najlepˇsia zhoda pre parameter L∗: • L∗ - lipa, poh´anka 1,3, mak 2, 3 , • korelaˇcn´y koeficient pre poh´anku 3, v´rbu 1,2 a 3, mak 2 a 3, ag´at a lipu je 0,65. Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 21 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Zhrnutie • Vyuˇzitie obrazovej anal´yzy - jednoduchost’, cena • 11 botanick´ych druhov obnˆoˇzok • Z´ıskanie farebn´eho rozptylu jednotliv´ych obnˆoˇzok • Urˇcenie homogenity/heterogenity na povrchu, v strede, ale i MeOH extrakte • Deklarovanie botanick´eho druhu Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 22 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT ˇDAKUJEM ZA POZORNOSˇT! Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 23 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Meranie farebn´eho rozptylu Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 23 / 23
Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Meranie farebn´eho rozptylu Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 23 / 23

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Martina Zivcakova - Opticke vlastnosti vcelyho pylu

  • 1. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Optick´e vlastnosti vˇcel´ıho pylu Autor: Martina ˇZivˇc´akov´a ˇSkolitel’ka: Ing. Anna Korb´aˇrov´a, Ph.D. ´Ustav fyziky a meˇric´ı techniky Vysok´a ˇskola chemicko–technologick´a v Praze 26.6.2014 Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 1 / 23
  • 2. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Osnova Obnˆoˇzkov´y pel’ Ciel’ pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 2 / 23
  • 3. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Obnˆoˇzkov´y pel’ Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 3 / 23
  • 4. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 4 / 23
  • 5. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Ciel’ pr´ace Meranie farebn´eho rozptylu a homogenity/heterogenity jednotliv´ych obnˆoˇzok z dan´eho botanick´eho druhu • inform´acia o farebnom rozptyle jednotliv´ych botanick´ych druhov • homogenita/heterogenita povrchu, stredu a obnˆoˇzky rozpustenej v MeOH Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 5 / 23
  • 6. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Ciel’ pr´ace Meranie farebn´eho rozptylu a homogenity/heterogenity jednotliv´ych obnˆoˇzok z dan´eho botanick´eho druhu • inform´acia o farebnom rozptyle jednotliv´ych botanick´ych druhov • homogenita/heterogenita povrchu, stredu a obnˆoˇzky rozpustenej v MeOH Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 5 / 23
  • 7. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Ciel’ pr´ace Meranie farebn´eho rozptylu a homogenity/heterogenity jednotliv´ych obnˆoˇzok z dan´eho botanick´eho druhu • inform´acia o farebnom rozptyle jednotliv´ych botanick´ych druhov • homogenita/heterogenita povrchu, stredu a obnˆoˇzky rozpustenej v MeOH Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 5 / 23
  • 8. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Ciel’ pr´ace Meranie farebn´eho rozptylu a homogenity/heterogenity jednotliv´ych obnˆoˇzok z dan´eho botanick´eho druhu • inform´acia o farebnom rozptyle jednotliv´ych botanick´ych druhov • homogenita/heterogenita povrchu, stredu a obnˆoˇzky rozpustenej v MeOH Meranie farebn´ych parametrov pomocou UV-VIS spektrometrie • porovnanie farebn´ych parametrov z´ıskan´ych UV-VIS spektrometriou a obrazovou anal´yzou Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 5 / 23
  • 9. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Ciel’ pr´ace Meranie farebn´eho rozptylu a homogenity/heterogenity jednotliv´ych obnˆoˇzok z dan´eho botanick´eho druhu • inform´acia o farebnom rozptyle jednotliv´ych botanick´ych druhov • homogenita/heterogenita povrchu, stredu a obnˆoˇzky rozpustenej v MeOH Meranie farebn´ych parametrov pomocou UV-VIS spektrometrie • porovnanie farebn´ych parametrov z´ıskan´ych UV-VIS spektrometriou a obrazovou anal´yzou Meranie antioxidaˇcnej kapacity • korel´acia farebn´ych parametrov s v´ysledkami merania antioxidaˇcnej kapacity Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 5 / 23
  • 10. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT CIE L∗ a∗ b∗ Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 6 / 23
  • 11. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Meranie farebn´eho rozptylu Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 7 / 23
  • 12. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Meranie farebn´eho rozptylu Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 8 / 23
  • 13. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Meranie farebn´eho rozptylu Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 9 / 23
  • 14. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Homogenita verzus heterogenita - Lipa (A) - obnˆoˇzka, (B) - povrch obnˆoˇzky, (C) - MeOH extrakt, (D) - stred obnˆoˇzky Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 10 / 23
  • 15. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Homogenita verzus heterogenita - Poh´anka 1 (A) - obnˆoˇzka, (B) - povrch obnˆoˇzky, (C) - MeOH extrakt, (D) - stred obnˆoˇzky Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 11 / 23
  • 16. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT UV-VIS spektrometria a obrazov´a anal´yza Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 12 / 23
  • 17. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT UV-VIS spektrometria a obrazov´a anal´yza Porovnanie parametru L∗ s OA a UV-VIS Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 13 / 23
  • 18. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT UV-VIS spektrometria a obrazov´a anal´yza Porovnanie parametru a∗ s OA a UV-VIS Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 14 / 23
  • 19. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT UV-VIS spektrometria a obrazov´a anal´yza Porovnanie parametru b∗ s OA a UV-VIS Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 15 / 23
  • 20. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Met´oda ABTS Princ´ıp met´ody ABTS HX−Fe3+ + H2O2 → X−[Fe4+ = O] + H2O ABTS R + X−[Fe4+ = O] → ABTS R ∗+ + HX−Fe3+ kde HX−Fe3+ je metmyoglob´ın, X−[Fe4+ = O] je ferrylmyoglob´ın, ABTS R je 2,2’-azinobis(3-ethyl-2,3-dihydrobenzothiazol-6-sulfon´at). Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 16 / 23
  • 21. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Met´oda ABTS Princ´ıp met´ody ABTS HX−Fe3+ + H2O2 → X−[Fe4+ = O] + H2O ABTS R + X−[Fe4+ = O] → ABTS R ∗+ + HX−Fe3+ kde HX−Fe3+ je metmyoglob´ın, X−[Fe4+ = O] je ferrylmyoglob´ın, ABTS R je 2,2’-azinobis(3-ethyl-2,3-dihydrobenzothiazol-6-sulfon´at). Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 16 / 23
  • 22. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Met´oda ABTS Princ´ıp met´ody ABTS HX−Fe3+ + H2O2 → X−[Fe4+ = O] + H2O ABTS R + X−[Fe4+ = O] → ABTS R ∗+ + HX−Fe3+ kde HX−Fe3+ je metmyoglob´ın, X−[Fe4+ = O] je ferrylmyoglob´ın, ABTS R je 2,2’-azinobis(3-ethyl-2,3-dihydrobenzothiazol-6-sulfon´at). Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 16 / 23
  • 23. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Met´oda ABTS Princ´ıp met´ody ABTS HX−Fe3+ + H2O2 → X−[Fe4+ = O] + H2O ABTS R + X−[Fe4+ = O] → ABTS R ∗+ + HX−Fe3+ kde HX−Fe3+ je metmyoglob´ın, X−[Fe4+ = O] je ferrylmyoglob´ın, ABTS R je 2,2’-azinobis(3-ethyl-2,3-dihydrobenzothiazol-6-sulfon´at). Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 16 / 23
  • 24. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Met´oda ABTS Princ´ıp met´ody ABTS HX−Fe3+ + H2O2 → X−[Fe4+ = O] + H2O ABTS R + X−[Fe4+ = O] → ABTS R ∗+ + HX−Fe3+ kde HX−Fe3+ je metmyoglob´ın, X−[Fe4+ = O] je ferrylmyoglob´ın, ABTS R je 2,2’-azinobis(3-ethyl-2,3-dihydrobenzothiazol-6-sulfon´at). Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 16 / 23
  • 25. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Met´oda ABTS Princ´ıp met´ody ABTS HX−Fe3+ + H2O2 → X−[Fe4+ = O] + H2O ABTS R + X−[Fe4+ = O] → ABTS R ∗+ + HX−Fe3+ kde HX−Fe3+ je metmyoglob´ın, X−[Fe4+ = O] je ferrylmyoglob´ın, ABTS R je 2,2’-azinobis(3-ethyl-2,3-dihydrobenzothiazol-6-sulfon´at). Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 16 / 23
  • 26. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Met´oda ABTS Princ´ıp met´ody ABTS HX−Fe3+ + H2O2 → X−[Fe4+ = O] + H2O ABTS R + X−[Fe4+ = O] → ABTS R ∗+ + HX−Fe3+ kde HX−Fe3+ je metmyoglob´ın, X−[Fe4+ = O] je ferrylmyoglob´ın, ABTS R je 2,2’-azinobis(3-ethyl-2,3-dihydrobenzothiazol-6-sulfon´at). Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 16 / 23
  • 27. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Met´oda ABTS Princ´ıp met´ody ABTS HX−Fe3+ + H2O2 → X−[Fe4+ = O] + H2O ABTS R + X−[Fe4+ = O] → ABTS R ∗+ + HX−Fe3+ kde HX−Fe3+ je metmyoglob´ın, X−[Fe4+ = O] je ferrylmyoglob´ın, ABTS R je 2,2’-azinobis(3-ethyl-2,3-dihydrobenzothiazol-6-sulfon´at). Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 16 / 23
  • 28. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Met´oda ABTS Princ´ıp met´ody ABTS HX−Fe3+ + H2O2 → X−[Fe4+ = O] + H2O ABTS R + X−[Fe4+ = O] → ABTS R ∗+ + HX−Fe3+ kde HX−Fe3+ je metmyoglob´ın, X−[Fe4+ = O] je ferrylmyoglob´ın, ABTS R je 2,2’-azinobis(3-ethyl-2,3-dihydrobenzothiazol-6-sulfon´at). Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 16 / 23
  • 29. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Meranie antioxidaˇcnej kapacity Pr´ıprava materi´alu • uk´aˇzka roztriedenia podl’a farebnosti pre mak, slneˇcnicu a v´rbu Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 17 / 23
  • 30. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Meranie antioxidaˇcnej kapacity Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 18 / 23
  • 31. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT V´ysledky Meranie farebn´eho rozptylu 1 Z´ıskali sme predstavu o farebnej jednote: • jednotn´a - facelia, • jednotn´e s odch´ylen´ymi bodmi - lipa, ag´at, poh´anka 1,2 a mak, • v¨aˇcˇs´ı rozptyl - d’atelina plaziv´a, poh´anka 3, • v¨aˇcˇs´ı poˇcet farebn´ych skup´ın - v´rba, repka, slneˇcnica. Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 19 / 23
  • 32. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT V´ysledky Meranie farebn´eho rozptylu 1 Z´ıskali sme predstavu o farebnej jednote: • jednotn´a - facelia, • jednotn´e s odch´ylen´ymi bodmi - lipa, ag´at, poh´anka 1,2 a mak, • v¨aˇcˇs´ı rozptyl - d’atelina plaziv´a, poh´anka 3, • v¨aˇcˇs´ı poˇcet farebn´ych skup´ın - v´rba, repka, slneˇcnica. 2 Na z´aklade farby sa n´am oddelili obnˆoˇzky do nasleduj´ucich farebn´ych skup´ın: • ˇzlt´a: lipa, mak, repka, ˇcast’ slneˇcnice a poh´anka 1, 2, • zelen´a: repka, ag´at, v´rba, ˇcast’ slneˇcnice a poh´anka 1, 2, • ˇcerven´a: poh´anka 3, ˇcast’ slneˇcnice a maku, • modrofialov´a: facelia. Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 19 / 23
  • 33. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT V´ysledky Porovnanie v´ysledkov z obrazovej anal´yzy a UV-VIS pre parametere L∗ , a∗ a b∗ vych´adzaj´uce z modelu CIE L∗ a∗ b∗ Najlepˇsia zhoda pre parametre: • L∗ - lipa, poh´anka 1, 2 a d’atelina plaziv´a, • a∗ - poh´anka 3, • b∗ - lipa, d’atelina plaziv´a a ag´at, • korelaˇcn´y koeficient pre parameter L* = 0,38, a* = 0,81 a b* = 0,68. Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 20 / 23
  • 34. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT V´ysledky V´ysledky porovnania parametru L∗ z modelu CIE L∗ a∗ b∗ z obrazovej anal´yzy z merania antioxidaˇcnej kapacity Najlepˇsia zhoda pre parameter L∗: • L∗ - lipa, poh´anka 1,3, mak 2, 3 , • korelaˇcn´y koeficient pre poh´anku 3, v´rbu 1,2 a 3, mak 2 a 3, ag´at a lipu je 0,65. Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 21 / 23
  • 35. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Zhrnutie • Vyuˇzitie obrazovej anal´yzy - jednoduchost’, cena • 11 botanick´ych druhov obnˆoˇzok • Z´ıskanie farebn´eho rozptylu jednotliv´ych obnˆoˇzok • Urˇcenie homogenity/heterogenity na povrchu, v strede, ale i MeOH extrakte • Deklarovanie botanick´eho druhu Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 22 / 23
  • 36. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT ˇDAKUJEM ZA POZORNOSˇT! Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 23 / 23
  • 37. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Meranie farebn´eho rozptylu Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 23 / 23
  • 38. Obnˆoˇzkov´y pel Ciel pr´ace Farebn´y rozptyl, homogenita vs. heterogenita UV-VIS spektrometria a obrazov´a anal´yza Meranie antioxidaˇcnej kapacity - met´oda ABTS V´ysledky a zhrnutie VˇSCHT Meranie farebn´eho rozptylu Martina ˇZivˇc´akov´a ´UFMT Optick´e vlastnosti vˇcel´ıho pylu 23 / 23